• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.588-592
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• 2007

The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.

• Journal of Veterinary Clinics
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• v.16 no.1
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• pp.218-221
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• 1999

A case of enetritis caused by Clostridium perfringens was observed in 4years old, male California sea lion(Zalophus californianus). Clinical signs were included in anorexia, depression, diarrhea and vomitting, and the animal died on 4 days after showing clinical signs. Grossly, bloody contents were presented in the intestine and the intestinal mucosa were congested and dark redcolored, and also there were serous fluid in the peritoneal cavity. Histopathologically coagulative necrosis destroyed two third of the villus. Clostridium perfringens were isolated from the intestinal contents. Based on the gross and histopathological findings, this case was diagnosed as necrotic enteritis caused by Clostridium perfringens infection.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.475-479
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• 2000

The purpose of this study was to investigate the cause of chronic diarrhea from anaerobic bacteria isolated from Siberian tiger with chronic diarrhea. Major anaerobic bacteria isolated from faces were Clostridium perfringens and their population was $6 {\times} 10^3$ cfu/g feces. Antibiotic sensi- tivity test against Clostridium perfringens was performed using 6 antibiotic drugs including colistin gentamicin, trimethoprim/sulfamethoxazole, amikacin, enofloxacin and penicillin. Clostridium per- fringens were sensitive to amikacin, enrofloxacin and penicillin while they were resistant to colistin. gentamicin and trmetboprim/sulfamethoxazole. And we found that chronic diarrhea of Siberian tiger was treated successfully with penicillin. These results suggested that Clostridium perfringens may bee a cause of chronic diarrhea in Siberian tiger.

• Korean Journal of Veterinary Research
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• v.33 no.3
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• pp.419-427
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• 1993

Thirteen strains of Clostridium perfringens were isolated from the pigs with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxin and susceptibility to antimicrobial agents of the isolates were investigated. The results could be summarized as follows ; 1. Almost of the pigs affected with hemorrhagic enteritis, 17 cases examined from 1989 to 1992, were piglets less than 7 day old. 2. The average mortality rate of piglet less than 7 day old affected with hemorragic and necrotic enteritis was 48.5%. 3. The clinical signs of pigs with hemorrhagic enteritis were depression, hemorrhagic diarrhea, anemia and dehydration. Necropsy of the infected pigs showed typical hemorrhage of upper intestine and necrosis of mucosal membrane. 4. The characteristic biochemical properties of the isolates were 2-band hemolysis, positive reaction of reverse CAMP test and formation of LV precipitate in egg yolk medium. 5. The toxin type of the 13 isolates, investigated by mouse inoculation test, was all type C strains of Clostridium perfringens. 6. In susceptibility test to antimicrobial agents, 13 isolates of Clostridium perfringens were highly sensitive to ampicillin, enrofloxacin(Baytril), cephalothin, penicillin and trimethoprim-sulfamethoxazole.

• Korean Journal of Veterinary Service
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• v.22 no.2
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• pp.151-158
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• 1999

This study was conducted to investigate the biochemical characteristics, the population and antibiotic susceptibility test of Clostridium perfringens isolated from intestinal contents of slaughter cattle in Kyung-ju and Po-hang. 1. In slaughter cattle Cl perfringens were isolated from intestinal contents of 51 of 101 cases(50.4%) and the population were $\leq$$10^5$cfu/ml of 44 cases(86.3%). 2. In antibiotic susceptibility test, ampicillin, bacitracin, cephalothin, penicillin polymyxin B were highly susceptible, ohloramphenicol, erythromycin, tetracycline were lowly susceptible, gentamicin, kanamycin, amikacin, streptomycin, sulfamethoxine, sulfamethazine were resistant. 3. In leaving test intestinal contents leaved for 0, 4, 8, 16, 32 hours in room temperature and population of Cl perfringens were gradually increased.

• Journal of Sericultural and Entomological Science
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• v.42 no.1
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• pp.6-9
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• 2000

This study was performed to investigate the catechins which separated from mulberry leaf. The Epicatechin contents in mulberry leves was 1.21${\pm}$0.05 $\mu\textrm{g}$/mg ethylacetate fraction. epigalocatechingallte contents WAS 3.56 $\mu\textrm{g}$/mg ethylacetate fraction. The cathechins (epicatechin and epigalocate-chingallate) separated from mulberry leaf was shown a strong activity to growth inhibition by the concentration of 5 mg/disc on the Clostridium perfringens.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.6
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• pp.826-832
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• 2023

Clostridium perfringens causes diarrhea and other diseases in humans and animals. We investigated the prevalence, toxin gene profiles, and antimicrobial resistance of C. perfringens isolated from commercial jeotgal sample. C. perfringens was isolated from 11 of 22 commercial jeotgals. All C. perfringens strains were positive for the alpha toxin gene, but not for the beta, epsilon, iota, CPE or NetB toxin genes; therefore, all strains were identified as type A C. perfringens. However, the beta2 toxin gene was identified in 54.5% of isolates. Disk diffusion susceptibility tests showed that most isolates were resistant to kanamycin (90.9%), nalidixic acid (72.7%), oxacillin (54.5%), erythromycin (27.3%), ciprofloxacin (9.1%) and clindamycin (9.1%). However, all strains were susceptible to 14 other antimicrobial including amoxicillin, ampicillin, and chloramphenicol. The average minimum inhibitory concentrations against C. perfringens of clindamycin, kanamycin, and nalidixic acid were 128.0, 128.0, and 54.0 ㎍/mL, respectively. These results provide new insight into the necessity for sanitation of commercial jeotgal, and provide evidence to help reduce the risk of contamination with antimicrobial-resistant bacteria.

• The Korean Journal of Food And Nutrition
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• v.11 no.3
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• pp.289-292
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• 1998

Identification and antibiotic susceptibility of Clostridium perfringens isolates from fecal specimens of healthy sheep and dogs were performed from December, 1995 to November, 1996 in Kwang-ju and Chonnam area. C. perfringens was isolated in 3 strains(15.0%) out of 20 healthy sheep and 2 strains(6.7%) out of 30 healthy dogs. In antibiotic susceptibility test of C. perfringens, 80% of the isolates was susceptible to ampicillin, baytril, and penicillin, 60% to cephalothin, and 40% to erythromycin.

• Applied Biological Chemistry
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• v.40 no.4
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• pp.267-270
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• 1997

To industrialize the foods using wormwood extract, the optimal extraction yield of growth-inhibitory component of wormwood (Artemisia princeps) against Clostridium perfringens in vitro was investigated at various conditions. The optimal extraction of wormwood was produced from the condition of $40^{\circ}C$, 4 hours and 100% ethanol (30 volume).

• Korean Journal of Veterinary Service
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• v.34 no.1
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• pp.31-35
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• 2011

This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by ${\alpha}$-toxin of C. perfringens A type.