• BMB Reports
• /
• v.48 no.12
• /
• pp.655-667
• /
• 2015

Lineage tracing is a widely used method for understanding cellular dynamics in multicellular organisms during processes such as development, adult tissue maintenance, injury repair and tumorigenesis. Advances in tracing or tracking methods, from light microscopy-based live cell tracking to fluorescent label-tracing with two-photon microscopy, together with emerging tissue clearing strategies and intravital imaging approaches have enabled scientists to decipher adult stem and progenitor cell properties in various tissues and in a wide variety of biological processes. Although technical advances have enabled time-controlled genetic labeling and simultaneous live imaging, a number of obstacles still need to be overcome. In this review, we aim to provide an in-depth description of the traditional use of lineage tracing as well as current strategies and upcoming new methods of labeling and imaging.

• The Journal of Korea Robotics Society
• /
• v.16 no.3
• /
• pp.283-290
• /
• 2021

We propose a task and motion planning algorithm for clearing obstacles and wiping surfaces, which is essential for surface disinfection during the pathogen disinfection process. The proposed task and motion planning algorithm determines task parameters such as grasping pose and placement location during the planning process without using pre-specified or discretized values. Furthermore, to quickly inspect many unit motions, we propose a motion feasibility prediction algorithm consisting of collision checking and an SVM model for inverse mechanics and self-collision prediction. Planning time analysis shows that the feasibility prediction algorithm can significantly increase the planning speed and success rates in situations with multiple obstacles. Finally, we implemented a hierarchical control scheme to enable wiping motion while following a planner-generated joint trajectory. We verified our planning and control framework by conducted an obstacle-clearing and surface wiping experiment in a simulated disinfection environment.

• KIEE International Transactions on Power Engineering
• /
• v.5A no.3
• /
• pp.300-302
• /
• 2005

We employ a new technique to account for extreme values when using the generalized autoregressive conditionally heteroskedastic (GARCH) methodology to forecast day-ahead electricity prices in New York City.

• Proceedings of the KIEE Conference
• /
• 2007.07a
• /
• pp.451-452
• /
• 2007

In this paper, we simulate power system transient stability using object-oriented programming(OOP). OOP is a more flexible method than procedual programming. There are several advantages in dynamic system simulation using OOP. We also calculate critical fault clearing time using energy functions for detailed models.

• Proceedings of the KIEE Conference
• /
• 1999.07c
• /
• pp.1239-1241
• /
• 1999

In this paper, we propose real time transient stability assessment and energy margin estimation using fuzzy approximate reasoning. The proposed method used rotor angle, kinetic energy and acceleration power of generators at clearing time as fuzzy input. In order to calculate energy margin in transient energy function (TEF), we obtained controlling unstable equilibrium point (UEP) using mode of disturbance procedure (MOD). The proposed algorithm is tested on 4-machine, 6-bus, 7-line power system to prove of effectiveness.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.21 no.3
• /
• pp.200-206
• /
• 2016

In the microgrid, inverters for energy storage system are generally constructed in a parallel structure because of capacity expandability, convenience of system maintenance, and reliability improvement. Parallel inverters are required to provide stable voltage to the critical load in PCC and to accurately share the current between each inverter. Furthermore, when islanding occurs, the inverters should change its operating mode from grid-connected mode to stand-alone mode. However, during clearing time and control mode change, the conventional control method has a negative impact on the critical load, that is, severe fluctuating voltage. In this study, a parallel operation control method is proposed. This method provides seamless mode transfer for the entire transition period, including clearing time and control mode change, and has accurate current sharing between each inverter. The proposed control method is validated through simulation and experiment.

• Journal of the Optical Society of Korea
• /
• v.17 no.1
• /
• pp.86-90
• /
• 2013

Various methods have been proposed for enhancing the contrast of laser speckle contrast image (LSCI) in subcutaneous blood flow measurements. However, the LSCI still suffers from low image contrast due to tissue turbidity. Herein, a physicochemical tissue optical clearing (PCTOC) method was employed to enhance the contrast of LSCI. Ex vivo and in vivo experiments were performed with porcine skin samples and male ICR mice, respectively. The ex vivo LSCIs were obtained before and 90 min after the application of the PCTOC and in vivo LSCIs were obtained for 60 min after the application of the PCTOC. In order to obtain the skin recovery images, saline was applied for 30 min after the application of the PCTOC was completed. The visible appearance of the tubing under ex vivo samples and the in vivo vasculature gradually enhanced over time. The LSCI increased as a function of time after the application of the PCTOC in both ex vivo and in vivo experiments, and properly recovered to initial conditions after the application of saline in the in vivo experiment. The LSCI combined with the PCTOC was greatly enhanced even in deep vasculature. It is expected that similar results will be obtained in in vivo human studies.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.17 no.1
• /
• pp.3627-3641
• /
• 1975

The Government is trying to increase total food grain yield to meet national self sufficiency by means of increasing unit yield as well as extending crop land, and this year he set the target of 321,000 hectare of forest to clear for cropping. This study was carried to investigate the most efficient method of clearing hillock by bulldozer, and successful method to develope yielding potential of newly cleared land in short term. Since the conventional land clearing method is just earth leveling and root removing neglecting top soil treatment, the growth of crop was poor and farmer tends not to care the land. The top-soil-furrowing method is applied through out this study, that is advantageous especially for the land having shallow top soil and low fertility like Korean forest. In this study, various operating method were tried to find out most efficient method separately in connection with the land slope less than 25 percent and over, and several fertilizing methods to develop yielding potential. The results are as follows; 1) For the natural land slope utilization method, applicable to the land having less than 25 percent slope, reverse operating was more efficient than using forward gear of bulldozer. The operating time was 3 hour 32 minutes and 36 seconds using forward gear was 2 hour 32 minutes and 30 seconds for reverse gear operation per 1,000 square meter. 2) Bulldozer having angle blade adjustment needed 7hr 15min. for constructing of terrace per 10a compaire with the one having angle & tilt adjustment needed 6hr 4min for same operations. Specially there is significant difference for operation time of first period (earth cutting) such as bulldozer having angle blade adjustment needed 3hr 56min compaired with the one having angle & tilt adjustment 3hr 59min. In construction of terrace, the bull-dozer having tilting and angle blade adjustment was most suitable and performed efficiently. 3) For the fertilizer application treatment, the grass (Ladino clover) yield in first year was almost same as ordinary field's in the plot applied(N.P.K+lime+manure) while none fertilizer plot showed one tenth of it, and (N.P.K.+lime) applied plot yielded on third. 4) The effect of different land clearing method to yield showed significant difference between each treatment especially in the first year, and the conventional method was the lowest. In the second year, still conventional terracing plot yielded only half of ordinary field while the other plots showed as same as ordinary field's. 5) The downward top soil treatment plot showed most rapid improvement in soil structure during one year physio chemically, it showed increase in pH rate and organic composition, and the soil changed gradually from loam to sand-loam and the moisture content increased against the pF rate, and it gives good condition to grow hay due to the increase of field water capacity with higher available water content. 6) Since the soil of tested area was granite, the rate of soil errosion was increased about 2 to 5 percent influencing in soil structure more sand reducing clay content, and an optimum contour farming method should be prepared as a counter measure of errosion.

• Proceedings of the KIEE Conference
• /
• 2015.07a
• /
• pp.545-546
• /
• 2015

Arc flash hazard study is required for the electric power system of industrial plant nowadays. Arc flash incident energy and level are calculated by arc flash hazard study. The arcing fault clearing time is determined by the response time of protection devices. This paper is protection devices coordination study to reduce potential incident energy for industrial electric power system.

• Journal of Plant Biotechnology
• /
• v.5 no.1
• /
• pp.13-17
• /
• 2003

Biotechnology is certainly one of the major landmarks in the 20th century history of science. It may produce enormous utility to human beings, but at the same time, it carries huge potential risks to the environment and public health. Thus, with a view to securing safety for the environment and public health in relation to the development and use of living modified organisms (LMOs), the Cartagena Protocol on Biosafety was adopted, in which a regulation Procedure as to the transboundary movements, transportation, handling, and use of LMOs were drawn up. In order to prepare for the entry into force of the Protocol, the Republic of Korea legislated the "Act on the Transboundary Movements of living modified organisms(hereinafter referred as the 'Act')" in Mar 2001, and has pre-announced the enactments of the enforcement ordinance and the enforcement regulation to the Act. Pursuant to the Act, the Ministry of Commerce, Industry and Energy, as a Competent National Authority, is making efforts to implement domestic biosafety schemes in cooperation with other bio-related government ministries. In order for these efforts to reap fruits, industry, academia, and research institutions should cooperate with one another, and civic groups and NGOs should narrow the differences in opinions and timely respond to the fast-changing situations. Focusing on the precautionary principle, the Protocol puts a great emphasis on the importance of information sharing amongst countries, and the Act also follows this principle. In order to gurantee biosafety, countries around the world, including the ROK, agreed to establish National Biosafety Clearing, designed to provide the information on the export/import of LMOs, R'||'&'||'Ds, risk assessment, safety control, etc. and register it on the CBD Central Biosafety Clearing House.e.