• 한국구조물진단유지관리공학회 논문집
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• 제13권5호통권57호
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• pp.169-179
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• 2009

본 논문의 목적은 열화상 비파괴 검사기법을 적용시 손상된 콘크리트 구조물의 표면온도를 증폭시키기 위해 사용되는 외부 열원의 효율성을 알아보기 위함이다. 원적외선램프와 할로겐램프의 적용성과 효율성을 서로 비교하였다. 이를 위해 전술한 두 개의 열원을 콘크리트의 내부공극과 FRP쉬트의 비부착 결함 시험체에 적용하였다. 본 연구결과, 원적외선램프가 할로겐램프보다 더 효율적인 것으로 파악되었다. 또한, 손상영역을 효과적으로 검출하기위해서 가우스 필터와 프리윗 마스크 화상처리기법을 혼합한 알고리즘을 제안하였다.

• 한국식품영양과학회지
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• 제30권5호
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• pp.964-968
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• 2001

향균력이 있는 한약제들 중에서 추출수율과 항균력이 우수한 항금을 대상으로 식중독균에 대한 천연항균제의 적용 가능성을 검토하였다. 황금추출물의 항균작용을 알아보기 위하여 공시균주 Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Vivro parahaemolyticus에 대하여 disk method에 의한 항균력 검사에서 생육어제로 인한 clear zone 이 선명하게 관찰되어 황금추출물의 항균력을 알 수 있었고, 황금추출물의 열 및 PH 안정성을 검토한 결과 다양한 범위의 온도 및 pH에 대해서 매우 안정하다는것을 확인하였으며, 공시균주들에 대한 유효저해농도를 측정한 결과 황금추출물의 농도 500 ppm 이상에서 생육이 억제되었다. 주사형전자현미경(SEM) 상에서는 처리구에서 공시균주들의 세포형태 변화를 볼수 있었고, 투과형전자현미경(TEM)상에서는 처리구에 있어서 공시균주들의 세포막 파괴로 인하여 세포내용물이 용출된 것을 볼 수 있었다. 황금추출물 처리로 인한 세포막 손상정도를 확인하기 위하여 균체내 효소인 $\beta$-gal-actosidase 활성을 정량한 결과 chlorogorm 보다 세포막을 더 손상시키는 것을 확인되었다.

• 디지털융복합연구
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• 제17권2호
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• pp.447-451
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• 2019

본 연구는 건강식품으로 많이 사용되는 32종의 약용식물에 대한 헬리코박터 파이로리 항균효과를 살펴보기 위해서 디자인되었다. 연구에 사용된 약용식물은 모든 사람들이 매일 먹을 수 있는 것으로 H.pylori 활성에 대한 디스크 확산법을 이용하여 32종의 약용식물 추출물을 사용하였고 Amoxicillin sodium (150mg/ml,일동제약)과 메트로니다졸 (Metronidazol, 50mg/ml)을 대조군으로 사용하였다. 투명한 영역의 면적을 측정하고 그 면적이 더 큰 크기가 H. pylori에 많은 효과가 있다고 평가했다. 이 연구의 결과, 고련피 억제 값은 $372.90mm^2$로 가장 높았고, 둘째, 오배자 $358.30mm^2$, 그리고 청호 $348.32mm^2$이었다. 양성 대조군인 Metronidazole (50mg / ml CJ) 값은 $503,29mm^2$이었다. 향후 H. pylori 억제를 위해 다양한 약용식물의 항균소재개발이 가치가 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.999-1010
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• 2021

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment. They are highly toxigenic and carcinogenic. Probiotic bacteria isolated from fermented foods were tested to check their ability to degrade and/or detoxify PAHs. Five probiotic bacteria with distinct morphologies were isolated from a mixture of 26 fermented foods co-cultured with benzo(a)pyrene (BaP) containing Bushnell Haas minimal broth. Among them, B. velezensis (PMC10) significantly reduced the abundance of BaP in the broth. PMC10 completely degraded BaP presented at a lower concentration in broth culture. B. velezensis also showed a clear zone of degradation on a BaP-coated Bushnell Haas agar plate. Gene expression profiling showed significant increases of PAH ring-hydroxylating dioxygenases and 4-hydroxybenzoate 3-monooxygenase genes in B. velezensis in response to BaP treatment. In addtion, both live and heat-killed B. velezensis removed BaP and naphthalene (Nap) from phosphate buffer solution. Live B. velezensis did not show any cytotoxicity to macrophage or human dermal fibroblast cells. Live-cell and cell-free supernatant of B. velezensis showed potential anti-inflammatory effects. Cell-free supernatant and extract of B. velezensis also showed free radical scavenging effects. These results highlight the prospective ability of B. velezensis to biodegrade and remove toxic PAHs from the human body and suggest that the biodegradation of BaP might be regulated by ring-hydroxylating dioxygenase-initiated metabolic pathway.

• 한국환경복원기술학회지
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• 제23권6호
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• pp.43-55
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• 2020

The study was performed in order to derive the management methods of revegetation space on embankment upper in the shoulder of expressway. The pilot study was conducted in 2013 on the test road section of the Jungbu Inland Expressway (Smart Highway) and continues to be monitored until 2020. In the test, three commonly used methods for weed control were applied. In the early two to three years, most of the methods were effective in controlling weeds. However, at the end of six years, weed suppression effects were different for each treatment. Vegetation coverage was 90% in the untreated control, 70-80% wood chip mulching method, 50-60% solidification method, and 20% sheet mulching method. The sheet method was found to be the most effective given the low vegetation coverage was effective in controlling weeds. The wood chip mulching method is promoting weed growth over time, and weeds are invading as the effect of soil hardening is reduced in the place where the soil hardener is treated. Among the methods applied in the test, mulching the sheet is the most effective, but it is important to use a durable sheet. In the future, it is necessary to find ways to control weeds on road shoulders, considering both economic and environmental aspects. For the proper management in the shoulder of expressway set target zone is needed. Clear standards for weed control on expressway should be established. And the technology to be applied must be durable for 3 years or more and must be able to suppress the amount of weeds to a level of 20% or less.

• 한국미생물·생명공학회지
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• 제50권2호
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• pp.245-254
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• 2022

Cellulases are a group of biocatalyst enzymes that are capable of degrading cellulosic biomass present in the natural environment and produced by a large number of microorganisms, including bacteria and fungi, etc. In the current study, we isolated, screened and characterized cellulase-producing bacteria from soil. Three cellulose-degrading species were isolated based on clear zone using Congo red stain on carboxymethyl cellulose (CMC) agar plates. These bacterial isolates, named as HB2, HS5 and HS9, were subsequently characterized by morphological and biochemical tests as well as 16S rRNA gene sequencing. Based on 16S rRNA analysis, the bacterial isolates were identified as Bacillus cerus, Bacillus subtilis and Bacillus stratosphericus. Moreover, for maximum cellulase production, different growth parameters were optimized. Maximum optical density for growth was also noted at pH 7.0 for 48 h for all three isolates. Optical density was high for all three isolates using meat extract as a nitrogen source for 48 h. The pH profile of all three strains was quite similar but the maximum enzyme activity was observed at pH 7.0. Maximum cellulase production by all three bacterial isolates was noted when using lactose as a carbon rather than nitrogen and peptone. Further studies are needed for identification of new isolates in this region having maximum cellulolytic activity. Our findings indicate that this enzyme has various potential industrial applications.

• 한국컴퓨터정보학회논문지
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• 제27권10호
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• pp.123-130
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• 2022

본 논문은 건설현장의 콘크리트 붕괴사고를 사전에 예방하기 위한 조치 중 하나로 감수율에 따른 콘크리트강도 저하에 영향을 미치는 일일 시간대별 변화와 온도의 변화를 시계열데이터로 축적된 기상청 자료를 기반으로 분석했다. 감수율 발생 구간의 예측을 확인할 신뢰성 있는 모델로 규칙적이고 명확한 시계열데이터 모델에 적합한 SARIMA모델을 통하여 p_value는 0.5 이하, coef는 일방향으로 나타나는 등 검증 항목들이 신뢰성 확보에 유의미한 결과를 얻었다. 이러한 신뢰를 바탕으로 확보한 데이터를 이용하여 시간대별 온도변화와 구간별 감수율을 분석한 결과 7~8월, 12~13시, 29~31℃ 구간이 가장 큰 감수율을 나타냄을 알 수 있다. 연구 결과를 이용하여 연구 결과 구간의 요인이 발생하면 배치플랜트에서 물-시멘트 배합설계 시 감수율을 반영한 레미콘을 생산하여 감수율에 따른 콘크리트 압축강도 저하를 예방할 수 있을 것으로 기대된다.

• 환경생물
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• 제41권2호
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• pp.167-178
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• 2023

Large amounts of waste and wastewater from aquaculture have negatively impacted ecosystems. Among them, shrimp aquaculture wastewater contains large amounts of nitrogen contaminants derived from feed residues in an aerobic environment. This study isolated candidate strains from adult rockworms to treat shrimp aquaculture wastewater (SAW) in an aerobic environment. Among 87 strains isolated, 25 grew well at the same temperature as the shrimp aquaculture with excellent polymer degradation ability (>0.5 cm clear zone). Six isolates (strains AL1, AL4, AL5, AL6, LA10, and PR15) were finally selected after combining strains with excellent polymer degradation ability without antagonism. 16S rRNA sequencing analysis revealed that strains AL1, AL4, AL5, AL6, LA10, and PR15 were closely related to Bacillus paramycoides, Bacillus pumilus, Stenotrophomonas rhizophila, Bacillus paranthracis, Bacillus paranthracis, and Micrococcus luteus, respectively. When these six isolates were applied to SAW, they reached a maximum cell viability of 2.06×10⁵ CFU mL^-1. Their chemical oxygen demand (COD_Cr) and total nitrogen(TN) removal rates for 12h were 51.0% and 44.6%, respectively, when the COD_Cr/TN ratio was approximately 10.0. Considering these removal rates achieved in this study under batch conditions, these six isolates could be used for aerobic denitrification. Consequently, these six isolates from rockworms are good candidates that can be applied to the field of aquaculture wastewater treatment.

• Educational Technology International
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• 제6권2호
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• pp.87-100
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• 2005

The purpose of this study lies in touching the study trend of Chinese education technology through the analysis on present situation of the study in Chinese education technology, and is to suggest the direction for the its further study and development. In order to achieve these kinds of study purpose, the frames have been set to grasp the features and trend of Chinese educational technology in such as 4 aspects of educational technology definition, curriculum and the cultivation of genuine individuals, study zone aspect and the method, and in order to grasp, in the comparison with the countries of advanced educational technology such as Korea, America etc., the progressive features and the trend of development of Chinese educational technology have been analyzed. As a result of the analysis, it has been found that Chinese educational technology has the different features in the study and development of educational technology of Korea and America in the abovementioned 4aspects. First, in the education technology comprehension, it differs from Korea, Japan and America, and understands the technology as a skill. Consequently, in either study or actuality, it seems puts more emphasis on skills (moreover, the aspect of hardware). Second, in the aspect of curriculum and the cultivation of the genuine individuals, gives preponderance to science aspect, and the unclear distinction between the education technology and the computer science makes that the true character of educational technology is not clear. Third, when speaking of study fields and study methods, those of china is a little behind of the advanced nations of the educational engineering, Korea, America and so on. And in the range of study, China is following the international tendency but when it comes to the depth of study and quality is very far from perfect. In the aspect of study method, the theoretical study, design study and development study are occupying mostly, and it is hardly to see the existence about actual proof study, and in this aspect, it is quite different than that of Korea and America. And it is judged that it is related with the traditional educational ideology and the academic study tradition, the cultural air and the features of economical development of China.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1781-1786
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• 2011

물리 화학적 처리에 의한 요구르트 오염균의 생육억제 효과를 알아보기 위해, 요구르트의 주요 오염균을 분리 동정하고, 열, pH, 전해수, 오존가스, microwave 처리 및 감마선을 조사하여 오염균주에 대한 사멸효과를 알아보았다. 오염된 요구르트로부터 분리한 효모의 지방산 조성 분석과 API(Analytic Profile Index) kit 분석을 실시한 결과, Hanseniaspora uvarum으로 동정되었으며 잠정적으로 Hanseniaspora uvarum Y1으로 명명하였다. H. uvarum Y1의 열 및 pH 처리에 의한 생육억제 효과를 측정한 결과, $70^{\circ}C$ 및 $80^{\circ}C$에서 15분 가열처리로 균이 사멸되었으며, pH 처리시 pH 2, 3 및 9에서 생육이 다소 억제되었으며, pH 1 및 10에서 완전히 억제되었다. 전해수 처리의 경우, clear zone이 5 mm 이상으로 H. uvarum Y1이 전해수에 높은 감수성을 가지는 것으로 나타났다. 오존가스 처리에 의한 H. uvarum Y1의 사멸효과를 측정한 결과, $10^2$ CFU의 균은 10분, $10^3$ CFU의 균은 20분 처리 시 모두 사멸한 것으로 나타났으며, microwave 처리의 경우, $10^6$ CFU 가량의 균이 1분 처리시 모두 사멸되었다. 방사선 조사의 경우, 균수를 90% 이상 감소시키는데 필요한 조사선량이 20 kGy 이상으로 H. uvarum Y1은 감마선에 저항성이 있는 균임을 알 수 있었다. 이상의 결과를 종합해볼 때 열, pH, 전해수, 오존가스, microwave 처리를 통해 요구르트 오염균주인 H. uvarum Y1의 생육을 효과적으로 억제할 수 있을 것으로 생각된다.