Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation, Characterization and Molecular Analysis of Cellulolytic Bacteria from Soil in Peshawar, Pakistan

  • Ikram, Hira (Institute of Biological Sciences, Sarhad University of Science and Information Technology) ;
  • Khan, Hamid Ali (Director Office of Reseaerch, Innovation and Commercialization, Sarhad University of Sciecnce and IT) ;
  • Ali, Hina (Institute of Biological Sciences, Sarhad University of Science and Information Technology) ;
  • Liu, Yanhui (Center for Genomics and Biotechnology, College of Life Sciences, Fujian Agriculture and Forestry University) ;
  • Kiran, Jawairia (Institute of Biological Sciences, Sarhad University of Science and Information Technology) ;
  • Ullah, Amin (Department of Health and Biological Sciences, Abasyn University Peshawar) ;
  • Ahmad, Yaseen (Institute of Biological Sciences, Sarhad University of Science and Information Technology) ;
  • Sardar, Sadia (Institute of Biological Sciences, Sarhad University of Science and Information Technology) ;
  • Gul, Alia (Department of Botany Hazara University)
  • Received : 2022.01.17
  • Accepted : 2022.05.19
  • Published : 2022.06.28
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Abstract

Cellulases are a group of biocatalyst enzymes that are capable of degrading cellulosic biomass present in the natural environment and produced by a large number of microorganisms, including bacteria and fungi, etc. In the current study, we isolated, screened and characterized cellulase-producing bacteria from soil. Three cellulose-degrading species were isolated based on clear zone using Congo red stain on carboxymethyl cellulose (CMC) agar plates. These bacterial isolates, named as HB2, HS5 and HS9, were subsequently characterized by morphological and biochemical tests as well as 16S rRNA gene sequencing. Based on 16S rRNA analysis, the bacterial isolates were identified as Bacillus cerus, Bacillus subtilis and Bacillus stratosphericus. Moreover, for maximum cellulase production, different growth parameters were optimized. Maximum optical density for growth was also noted at pH 7.0 for 48 h for all three isolates. Optical density was high for all three isolates using meat extract as a nitrogen source for 48 h. The pH profile of all three strains was quite similar but the maximum enzyme activity was observed at pH 7.0. Maximum cellulase production by all three bacterial isolates was noted when using lactose as a carbon rather than nitrogen and peptone. Further studies are needed for identification of new isolates in this region having maximum cellulolytic activity. Our findings indicate that this enzyme has various potential industrial applications.

Keywords

Acknowledgement

We are thankful to Dr. Hina Ali for her assistance in Bioinformatics and the organization of manuscript and Mr. Shafiq for his helping in experimental lab work and all the authors for contribution in this manuscript. This study was partially supported by Sarhad University of Science and IT, Peshawar, Pakistan.

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