• Microbiology and Biotechnology Letters
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• v.21 no.6
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• pp.542-548
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• 1993

Bacillus stearothermophilus was shown to express multiple xylanolytic enzymes including acetyl xylan esterase. Genomic DNA of the strain partially digested with HindIII was ligated into the HindIII site of pBR322, and expressed in E. coli HB101 cells in order to clone the gene for acetyl xylan esterase. One transformant among 4000 screened formed a clear zone around its colony on the LB agar supplemented with 1.0% tributyrin. The functional clone harbored the recombinant plasmid pKMG5 with an insert of 5.1kb.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2002.10a
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• pp.497-504
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• 2002

The characteristics of harmonic phase angles and phase angle differences contained in earthquake ground motions such as El Centre 1940 NS, Taft1 1952 NS, Hachinohe 1968 NS and Mexico 1985 are figured, which have been mostly overlooked in contrast with the importance placed on harmonic amplitudes. Recently, performance based design method is used for seismic design and seismic retrofitting, which needs nonlinear response analysis, there must be earthquake ground accelerations which contain the phase angle and the phase angle difference characteristics of the zone considered to be constructed building structures. To make clear the importance of phase angle differences, 4-earthquake ground motions are normalized by 200 gal and nonlinear response characteristics of normalized 4-earthquake ground motions are compared.

• Korean Journal of Materials Research
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• v.14 no.4
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• pp.239-245
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• 2004

Resistance spot weldability of coated steels for automobile applications was investigated. Test samples were prepared from commercial products of 0.8 mm in thickness. Based on the tensile-shear test, surface quality examination and cracking behavior, it was clear that aluminized steels showed good weldability. Microstructural inspection revealed that the coated materials was piled up at the split zone in the welding of aluminized steels. It was also demonstrated that no weld crack was found in the aluminized steel weld metal even the welding was carried out with higher current. However, through thickness cracks were detected at the weld metal of zinc coated steel. Small particles were found on the crack surface of zinc coated steel weld metal. It was thought that zinc vapor played key role to form the weld crack.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.244-251
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• 1998

To understand the effect of supplement of water extract of pine needle(WEPN) on shelf-life enhancement of the kimchi, activities of four enzymes and number of lactic acid bacteria, during fermentation of the kimchi, were assayed. Enzyme activities of kimchi fermented for 7 days with supplement by 2% water extract of pine needle showed amylase of 86.4%, protease of 85.8%, polygalacturonase of 61.5% and $\beta$-galactosidase of 58.8% against the control kimchi. WEPN showed weak inhibitory effect when it was applied to the isolated enzymes in vitro then those menifested by the kimchi in vivo. Number of total bacterial cell of WEPN supplemented kimchi increased by 10 folds than control between 7 to 14 days of fermentation. On contrast, number of lactic acid bacteria decreased maximaly to 21% of control by fermentation. The clear zone formed on paper disk by WEPN against L. plantarum was larger than that of Leu. mesenteroides.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2003.10a
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• pp.479-486
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• 2003

The characteristics of harmonic phase angles and phase angle differences contained in earthquake ground motions such as El Centre 1940 NS, Taft1 1952 NS, Hachinohe 1968 NS and Mexico 1985 are figured, which have been mostly overlooked in contrast with the importance placed on harmonic amplitudes. Recently, performance based design method is used for seismic design and seismic retrofitting, which needs nonlinear response analysis, there must be earthquake ground accelerations which contain the phase angle, the phase angle difference and energy input spectrum characteristics of the zone considered to be constructed building structures. To make clear the importance of phase angle differences, responses of 4 recorded earthquake ground motions, 4-earthquake ground motions normalized by 110 gal and 4 artificial earthquake motions compatible to the seismic building code of Korea are compared.

• Korean Journal of Plant Resources
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• v.24 no.6
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• pp.719-723
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• 2011

Antimicrobial activities of methanol, ethanol, water, and $CH_2Cl_2$ extracts from Fritillaria unibracteata Hsiao et K.C. Hsia and F. ussuriensis Maxim. were investigated by disk-agar diffusion method. The result showed comparatively strong antimicrobial activity against several microorganisms. The extracts from F. unibracteata and F. ussuriensis dosedependently increased the activity. However, water and $CH_2Cl_2$ extracts showed no antimicrobial activity against 7 microorganisms. Especially, against the most sensitive microorganism Staphylococcus epidermidis, methanol extracts at highest concentration of 20 mg/mL exhibited the largest clear zone on plate by 6-12 mm and ethanol extracts on plate by 6-10 mm.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.1
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• pp.129-132
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• 2012

Silk fibroin films incorporated with tetracycline was prepared and characterized by Fourier-transform infrared spectrometer and differential scanning calorimeter and examined antibacterial effect. The conformation of silk fibroin was changed from random coil to $\hat{a}$ sheet structure with incorporation of tetracycline. Antibacterial activity of the materials was evaluated against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus epidermis, and Staphylococcus aures) using agar plate method showing clear inhibition zone around tetracycline silk film. It is concluded that tetracycline-incorporated SF films are highly effective against bacteria.

• Proceedings of the KIEE Conference
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• 1995.07a
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• pp.150-152
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• 1995

In order to study the basic I-V behavior and quench characteristics, we fabricated two kinds of small superconducting coils which were wound with different superconductors. In this study, it was made clear that the propagation. velocity of normal zone preferentially depends on the operation current rather than heat pulse energy and also increases exponentially with respect to operation current.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.11
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• pp.1629-1636
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• 2004

The continuous casting is primarily a heat-extraction process in which the heat transfer at various cooling zones profoundly influences quality of products. So development of numerical model is necessarily needed for more specific and clear investigations upon heat transfer mechanism at mold and secondary cooling zones. In this study, heat transfer coefficients which show the characteristic of heat transfer mechanism in mold are calculated for more exact analysis with temperature measured in bloom mold using optimal algorithm, and finally the validity of cooling conditions at secondary cooling zone actually used at field fur 30 Ton bloom type continuous casting of 0.187%C is investigated. From the results of solidification analysis, the characteristic of bloom mold shows a similar tendency with that of previous studies, and optimized cooling conditions for 0.187%C are presented.

• Journal of Integrative Natural Science
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• v.4 no.1
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• pp.15-22
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• 2011

In this study, liquid with carbonized artemisia was developed for the first time that can be used as food additives and outside medicinal agents by burning artemisia species such as Artemisia dubia wall, Artemisia capillaris Thunb, Artemisia montana (Nakai) Pamp), Artemisia keiskeana Miq, Leonurus Japonicus Houtt. etc. In addition, edibility of recovered liquid with carbonized artemisia was verified by comparing the contents according to each ingredient with common refined pyroligneous liquid through the analysis of 9 kinds of organic acid by the species of artemisia, methanol and ethanol. Besides, the result of antimicrobial activities on six kinds of food spoilage and food poisoning bacteria observed by paper disc method for liquid with carbonized artemisia indicated that those two liquids showed strong antimicrobial. At the concentration of 1.5 mg/disc, clear zone for S. sonnei and S. aureus, L. monocytogenes was 13~16 mm, and they showed stronger antimicrobial activities than other strain.