• Korean journal of applied entomology
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• v.18 no.2 s.39
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• pp.107-110
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• 1979

An parasite, Anicetus beneficus was introduced from Japan to Jeju-do, Korea in 1975, and evaluated as a biological agent for control of the ruby scale, Ceroplastes rubens MASKELL. The results were as follows: 1. A major pest of citrus, ruby scale had one generation a year and hatched from late June to early August with the peak hatching in the middle of July. 2. A. beneficus was widely distributed in 16 citrus growing areas since released in 1975. 3. The pnupulation of ruby scales was considerably decreased by increasing of the parasites. Mean percent parasitism of A. beneficus was 1.7 in 1975, 14.1 in 1976, 31.0 in 1977 and 37.0 in 1978.

• Journal of Environmental Science International
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• v.29 no.10
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• pp.997-1002
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• 2020

In this study, we investigated the effects of the eco-friendly chemical bio-sulfur, on the citrus melanose-causing pathogen, Diaporthe citri, and on the pest, Panonychus citri. In an open field experiment with a plot-scale application of the chemicals: Mancozeb, lime sulfur, lime sulfur + machine oil, and bio-sulfur, the control group showed 70.6% disease severity compared with 10.3% for the Mancozeb-treated group. Among the eco-friendly treatments, disease severity was the lowest for the group treated with lime sulfur + machine oil (32.2%) and was 53.9%, 58.8%, and 58.1% following treatment with lime sulfur, and bio-sulfur diluted 500 and 1000 times, respectively. The proportion of diseased fruit showed similar results, suggesting that bio-sulfur is an effective alternative to lime sulfur. Three days after treatment acaricidal effects on P. citri showed a 197.6% control survival rate whereas the machine oil, and bio-sulfur diluted 500 and 1000 times treatments showed rates of 2.9%, 5.8%, and 9.0%, respectively. After three days, the control value for bio-sulfur diluted 1000 times was 73.2% compared with the values for the machine oil (96.4%) and bio-sulfur diluted 500 times (94.6%) treatments. Therefore, we suggest that additional research is needed on the combined application of bio-sulfur and oils to enhance the additive control effect on citrus melanose and Panonychus citri.

• Korean Journal of Environmental Agriculture
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• v.20 no.3
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• pp.186-191
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• 2001

Recently, petroleum spray oil(PSO) has been used to control key pests in integrated pest management (IPM) of citrus and other orchards in Australia and USA. In order to clarify the influences of a newly developed PSO (D-C Tron $Plus^{(R)}$) on citrus leaves, 0.33% or 1.0% of PSO were sprayed to potted 4-year-old citrus trees under some kinds of condition, and then the changes of photosynthesis, transpiration, stomatal conductance and chlorophyll fluorescence(Fv/Fm) were determined. When sprayed with 1.0% PSO, the photosynthetic rate, transpiration and stomatal conductance of citrus leaves were decreased by 20%, and then recovered in 20 days after treatment (DAT), while there were little influences by the spray of 0.33% PSO. The value of Fv/Fm decreased more under the $34/24^{\circ}C$ temperature condition than that of under the $30/20^{\circ}C$ and $28/16^{\circ}C$ condition. The high temperature ($50^{\circ}C$ for 10 hours)-treated trees sprayed with PSO 1.0% or PSO 1.0% plus dithianon 1/2000 dilution showed not only the increase of rate in dropped leaf but also the reduced photosynthesis and Fv/Fm compared with $30/20^{\circ}C$ temperature-treated ones. From the results of this study, the spray of 1.0% PSO can inhibit the physiological activities in citrus leaf, particularly under high temperature condition after spray or the mixing-spray with a fungicide (dithianon WP, 75%).

• Journal of Microbiology and Biotechnology
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• v.19 no.8
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• pp.749-759
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• 2009

A survey of Bacillus thuringiensis (Berliner) strains isolated from Spanish citrus orchards has been performed, and the strains were tested for insecticidal activity against the Mediterranean fruit fly Ceratitis capitata (Wiedemann), a key citrus pest in Spain. From a total of 150 environmental samples, 376 isolates were selected, recording a total B. thuringiensis index of 0.52. The collection was characterized by means of phase-contrast microscopy, SDS-PAGE, and PCR analysis with primer pairs detecting toxin genes cry1, cry2, cry3, cry4, cry5, cry7, cry8, cry9, cry10, cry11, cry12, cry14, cry17, cry19, cry21, cry27, cry39, cry44, cyt1, and cyt2. Diverse crystal inclusion morphologies were identified: bipyramidal (45%), round (40%), adhered to the spore (7%), small (5%), and irregular (3%). SDS-PAGE of spore-crystal preparations revealed 39 different electrophoresis patterns. All primer pairs used in PCR tests gave positive amplifications in strains of our collection, except for primers for detection of cry3, cry19, cry39, or cry44 genes. Strains containing cry1, cry2, cry4, and cry27 genes were the most abundant (48.7%, 46%, 11.2%, and 8.2% of the strains, respectively). Ten different genetic profiles were found, although a total of 109 strains did not amplify with the set of primers used. Screening for toxicity against C. capitata adults was performed using both spore-crystal and soluble fractions. Mortality levels were less than 30%. We have developed a large and diverse B. thuringiensis strain collection with huge potential to control several agricultural pests; however, further research is needed to find out Bt strains active against C. capitata.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.415-425
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• 2013

The yellow tea thrips, Scirtothrips dorsalis (Thysanoptera: Thripidae), has been regarded as a minor pest on citrus on Jeju Island. However, the damage of yellow tea thrips has gradually increased since 2007. This study was conducted to develop a forecasting model for generation peak time of S. dorsalis by using degree-days. Simple linear regression analysis was applied to determine the relationship between the generation number (x, dependent variable) and degree-days (y, independent variable). As a result, two regression models were established: citrus-based model (y = 310.9x + 69.0, $r^2$=0.99) and green tea-based model (y = 285.7x + 84.1, $r^2$=0.99). The models was fitted by independent data sets obtained from 2013 and evaluated using the technique of RSS (residual sum of square) and ${\chi}^2$-test. The green tea based-model showed a good fitting ability. The discrepancy between model outputs and actual data, and the practical application of models were discussed.

• Korean journal of applied entomology
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• v.60 no.1
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• pp.1-13
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• 2021

The concept of integrated pest management (IPM) first developed in the 1950s, and the concept of economic control via pest management was established in the 1960s. Research on IPM began in the United States and Europe, and IPM studies in Korea started with citrus insects and paddy field pests following the distribution of high-yield varieties of rice. Apple IPM in Korea began with research on pest control using chemical pesticides and pesticides resistant to insect pests, studies on the ecology of insect pests and their natural enemies, and the exploitation of sex pheromones on insect pests. Since the 1990s, IPM research and field projects have been carried out simultaneously for farming households. In the 2000s, the development of pest monitoring and forecasting models centered on mating disturbances, database programs for pests, and networks for sharing information. IPM technology has expanded via the development of unmanned forecasting systems and automation technologies in the 2010s.

• Korean journal of applied entomology
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• v.50 no.1
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• pp.55-63
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• 2011

This study was conducted to develop an artificial diet for the mugwort looper, Ascotis selenaria (Lepidoptera: Geometridae), which is an insect pest to leaves of citrus (Citrus unshiu). Corn and soybean powder were selected as main nutrient sources for larvae of A. selenaria after several diets consisted of wheat germ, corn, kidney bean and/or soybean were tested for larval development and survival. A higher amount of the main nutrients in the diet increased the larval survivorship. Addition of yeast and cholesterol in diet increased the larval survivorship. Finally the composition of diet was decided as followings; corn 100 g, soybean 100 g agar 25 g, Brewers' yeast 30 g, cholesterol 0.5 g, Vanderzant vitamin mixture 2 g, Wesson's salt mixture 2 g, sorbic acid 2 g, ascorbic acid 2 g, and methyl-4-hydroxybenzoate 2.5 g, and distilled water 1 liter. Development periods of larvae and pupae, survival rate and fecundity of A. selenaria reared on the diet were not significantly different with those on the host plant, citrus leaves. Larvae of early instars were reared in a group, while larvae of later instars (5-6th) were reared individually. Adult mating was conducted in a plastic cage and an oilpaper covered with a gauze was provided as an oviposition site.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.243-245
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• 2016

The insecticidal toxicities of seven essential oils against Ricania sp. nymph and adults, newly recorded pest, were evaluated using leaf-dipping and spray methods. At 72 h exposure time, the $LC_{50}$ values of the essential oils of Artemisia vulgaris, Anethum graveolens, Citrus paradisi, Melaleuca alternifolia, Melaleuca leucadendron, Neroli bigarade and Tagetes erecta were 251.02, 387.31, 286.21, 353.54, 275.64, 424.55, $165.68mg/cm^2$ when using the leaf dipping method against Ricania sp. nymphs, and 278.31, 402.11, 306.11, 379.64, 298.22, 459.56, $183.21mg/cm^2$ when using the spray method against Ricania sp. adults, respectively. These results indicate that the essential oil of the Tagetes erecta can be potentially used as new natural insecticidal agents against Ricania sp. nymph and adults.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.1
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• pp.12-24
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• 2023

Sitophilus oryzae L. (Rice Weevil) is a stored pest of rice that causes extensive loss throughout the world. We tested the leaf powders of 12 plant species viz., Chrysanthemum sp., Cinnamomum zeylanicum, Citrus grandis, Citrus limon, Gliricidia sepium, Gymnema sylvestre, Hemigraphis colorata, Michelia champaca, Moringa oleifera, Murraya koenigii, Polyalthia longifolia, and Sauropus androgynus at dosages of 1.00, 1.50, 2.00 and 3.00g against the adult rice weevil and mortality was recorded at 1, 2, 3, 5, 7, 12 and 2l days after treatment (DAT) by direct contact toxicity for their adulticidal effect. We observed 100 percent adult mortality in C. zevlanicum and M. koenigii among the tested leaf powders. In addition, the first-time tested H. colorata and S. androgynus also caused high mortality compared to other plants. All the plant powders caused moderate to high adult mortality. Hence, these plants could be effective botanical insecticides against S. oryzae as they comprise a potential source of bioactive chemicals and are generally free from toxicants. Applications of these natural derivatives in S. oryzae control could reduce the cost of control methods and storage of rice contamination. Therefore, the present study indicates that some plant extracts can be used as an alternative to toxic synthetic chemicals in the management of rice weevils.

• Natural Product Sciences
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• v.26 no.3
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• pp.207-213
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• 2020

Characterization and in vitro inhibition studies of protease inhibitor from the mushroom Pleurotus floridanus (PfTI) towards the pest Papilio demoleus is studied. The addition of 1 mM Mn²⁺, Na²⁺, Ba²⁺ and Ni ²⁺ enhanced the PfTI activity. The ICP-atomic emission spectrum showed the presence of Ca²⁺, Mg²⁺ and Zn²⁺ in the PfTI. Surfactants SDS and CTAB at a concentration of 1% reduced the PfTI activity whereas, the nonionic detergents Triton X and Tween 80 increased the activity. The inhibitory activity gradually decreased with increase in concentration of DMSO and H₂O₂. The activity was increased by dithiothreitol up to a concentration of 80 μM and inactivated at 140 μM. The activity of PMSF modified PfTI was drastically reduced to 0.234 U/mL at 4 mM concentration and similar results were obtained for modification of cysteine by N-Ethylmaleimide at slightly higher concentrations. The complex of trypsin and PfTI showed complete loss in fluorescence intensity at 343 nm compared with control. In vitro inhibition studies of PfTI with midgut proteases isolated from citrus pest P. demoleus with protease activity of 1.236 U was decreased to 0.613 U by 50 μL (0.1 mg/mL) of the inhibitor. Inhibitor was stable up to 0.04 M concentration of HCl.