• Title/Summary/Keyword: Circulation Point

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Purification and Characterization of a Methanol Dehydrogenase Derived from Methylomicrobium sp. HG-1 Cultivated Using a Compulsory Circulation Diffusion System

  • Kim, Hee-Gon;Kim, Si-Wouk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.134-139
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    • 2006
  • Methanotrophs are microorganisms that possess the unique ability to utilize methane as their sole source of carbon and energy. A novel culture system, known as the compulsory circulation diffusion system, was developed for rapid growth of methanotrophic bacteria. Methanol dehydrogenase (MDH, EC 1.1.99.8) from Methylomicrobium sp. HG-1, which belongs to the type I group of methanotrophic bacteria, can catalyze the oxidation of methanol directly into formaldehyde. This enzyme was purified 8-fold to electrophoretic homogeneity by means of a 4 step procedure and was found in the soluble fraction. The relative molecular weight of the native enzyme was estimated by gel filtration to be 120 kDa. The enzyme consisted of two identical dimers which, in turn, consisted of large and small subunits in an ${\alpha}_2{\beta}_2$ conformation. The isoelectric point was 5.4. The enzymatic activity of purified MDH was optimum at pH 9.0 and $60^{\circ}C$, and remained stable at that temperature for 20 min. MDH was able to oxidize primary alcohols from methanol to octanol and formaldehyde.

Toinsul and Oriental Physical Therapy (도인술(導引術)과 한방 물리치료)

  • Bae Ju-Han;Kim Sang-Su
    • The Journal of Korean Physical Therapy
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    • v.10 no.2
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    • pp.195-200
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    • 1998
  • Toinsul consists of breathing method and exercising method by stimulation of acupuncture point and meridian. It activates the circulation of energy and blood and helps excretion of noxious vapor and it improves elasticity and vitality of muscles, tendon md ligament. It sharpens both secretion of synovia .and circulation around joints and also controls the whole body through meridian. This report introduces some about the exercise methods for treatment of lumbago and shoulder joint diseases among medical, surgical and gynecological diseases. It can be used for health care, treatment, relaxation muscles and recovery from fatigue in an aspect of prevention. It has been widely used for treatment of diseases and promotion of health so far even though it is not yet demonstrated scientifically. Therefore, it should be used mere efficiently as an important part of the oriental physical therapy.

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Effect of Air Circulation Velocity on the Rate of Lumber Drying in a Small Compartment Wood Drying Kiln (소형 목재인공건조실에 있어서 공기순환속도가 목재건조율에 미치는 영향)

  • Chung, Byung-Jae
    • Journal of the Korean Wood Science and Technology
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    • v.2 no.2
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    • pp.5-7
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    • 1974
  • 1. This study indicates that above the fiber saturation point the drying rate can be increased with increasing the velocity of the air circutation, i.e., the drying rate of sample boards is proportional to the air velocity, but below the fiber saturation point, the effect of the velocity of air circulation is very low as shown in Figs. 1 and 2. 2. Under the controlled temperature and humidity in the kiln, the more the sample boards have moisture, the higher drying rate of it can be obtained. In other words, this means that even though in the case of drying various moisture content of wood, at the final drying stage, approximately the same percentage of moisture content of wood can be secured by employing the higher velocity of air circulation. 3. This study shows that the rate of drying in kiln changes distinctly at the fiber saturation point, i, e., above the fiber saturation point, the drying curve shows concave aginst the X axsis, but below the fiber saturation point, in the range from 30 percent of moisture content to 20 percent of moisture content, the curve shows convex as shown in Fig. 3. As the drying progresses, however, the drying curve shows concave again below 20 percent of moisture content. This means that inflection point of drying curve may be located clearly at the fiber saturation point, i.e., 30 percent of moisture content. As mentioned above, the 30 percent of moisture content of wood at which the inflectional point appears can be recognized as a critical point, i. e., the fiber saturation point at which all free water was removed from wood. The existence of inflectional point indicates that the evaporation of hygroscopic water in a cell wall is more difficult than the evaporation of free water in a cell cavity and the minor space of cell wall. The convex curve in the range of moisture content from 30 percent to 20 percent means that the evaporation of capillary condensed water has a tendency of the same rates of drying approximately, but as approaching to the 20 percent of moisture, the transfusion of moisture from wood becomes difficult because of having less moisture in cell wall. Below 20 percent of moisture content, the drying curve shows concave again, which means that it is difficult to remove the moisture located nearer to the surface of cellulose molecules and the surface bound water. These relations were revealed in Fig. 4. In comparison AC curve which does not have the two inflection points with BD curve which has two inflection points, i.e., Band D, they are mentioned already, by existence of the inflection points, the curve BD shows that the change of drying rate in the interval from 20 percent of moisture content to 30 percent of moisture content is not greater than in the case of the curve AC in the same interval. At the inflection point of 30 percent of moisture content, it can be noticed that the changing of the drying rate is very conspicuous. This phenomenon also can be recognized, as it is noticed by the Fig. 3, the drying rate from green to 30 percent of moisture content is very great. But the inclination of the curve is very slow from 30 percent of moisture content to 20 percent of moisture content, i.e., the inclination of the curve becomes almost horizontal lines. Acknowledgments Gratitude is expressed to Fred E. Dickinson, Professor of 'Wood Technology, School of Natural Resources, University of Michigan, USA for his suggestion to carry out this study.

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A Study on Information Breakdown through the Analysis of Industrial Engineering EPC Business Process (산업설비 EPC 업무 분석을 통한 정보 분류 내역에 관한 연구)

  • Cho, Hang-Min;Song, Young-Woong;Choi, Yoon-Ki
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2006.11a
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    • pp.167-172
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    • 2006
  • It follows recently in an order augmentation of overseas construction market plant field and the project of oil gas field is increasing. Consequently, the hazard which secures the competitive power of the overseas plant enterprise of the domestic building industry enterprises the technical competitiveness security which stands, productivity improvement and strategy management propulsion back positive disposal plan establishment are demanded. Even from in that oil gas field of overseas plant enterprise for an industrial competitive power improvement the application of EPC circulation information which relates with a project accomplishment is more demanded, the EPC phased star efficiently it manages the circulation information contents which occurs from the business which is various it is not presented it cannot there is a contents classification system for. If with him about lower the draft the problem point of information omission back of occurrence and business subject for of the fringe land and duplication business occurs, about lower the productivity decrease actual condition is appearing with such problem point. The research which it sees consequently in order to improve the use characteristic of the augmentation of practical application of the contents management system of oil gas field and building industry information civil official establishhes the contents management system the BPM for (Business Process Management) to present the contents classification system of base, it does to sleep. The part of Business Process materiality the knowledge which is demanded, contents information and system anger it analyzes system anger of industrial equipment circulation information it takes a triangular position and to sleep it does.

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Control of heparinization by activated clotting time during extracorporeal circulation (개심술시 Activated Clotting Time 을 이용한 Heparin 투여 조절에 관한 임상적 고찰)

  • 서충헌
    • Journal of Chest Surgery
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    • v.16 no.3
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    • pp.281-288
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    • 1983
  • Heparinization is an essential step in extracorporeal circulation for open heart surgery. But wide individual variation to heparin effect sometimes makes it difficult to anticoagulate safely or neutralize appropriately. Because the conventional set protocol of heparinization did not consider this individual variation, a new method of control of heparinization was proposed by Dr. Brian Bull in 1974. We compared the group in which a conventional set protocol was used [Control group] with the other in which a new protocol modified from that of Bull was used [ACT group], on the aspects of the dosages of heparin and protamine administered and postoperative bleeding. Our conventional protocol [Control group] consisted of: 1. Initial heparin was given at dose of 350U/Kg into the right atrium prior to bypass. 2. Additional heparin was given every hour during E.C.C., as much as a half of the Initial dose. 3. 600U of heparin was mixed into every 100ml. of priming solution. 4. The protamine dose was calculated by totalling the units of heparin given to the patient and giving 1 .8mg. of protamine per 100 units of heparin. ACT protocol [ACT group] consisted of: 1. Initial heparinization was same as that of conventional protocol. 2. ACT`s were checked before [A point] and 10 minutes after initial heparinization [B point]. With these 2 points, a dose response curve was drawn. 3. Heparin for the priming solution was same as in control group. 4. Every 30 minutes during E.C.C., ACT`s were checked with Hemochron [International Technidyne Corp.]. ACT between 450 and 600 seconds was regarded as safety zone. If ACT checked at a time was below 450 seconds, heparin dose was calculated on the dose-response curve to lengthen ACT to 480 seconds and was given into the oxygenator. 5. About 10 minutes before the term of E.C.C., ACT was checked to estimate the blood heparin level at the time. Then, protamine dose was calculated at dose of 1.Stag per 100 units of heparin. The calculated dose of protamine was mixed into 50 to lO0ml of 5% Dextrose Water and dripped intravenously during the period of 15 minutes. Compared these two groups mentioned above, results were obtained as follows: 1. Mean value of normal ACT checked with Hemochron on 30 preoperative patients was 124 seconds [range 95-145 sec.]. 2. Doses of heparin and protamine given to the patient were decreased in ACT group as much as 32.2% and 62.2% respectively. 3. Postoperative bleeding and transfusion were also decreased in ACT group in 60.5% and 67.1% respectively. 4. Our modified dose-response curve did not cause any problems in the control of heparinization. 5. Initial heparinization [Heparin 350U/Kg] was sufficient for the most patients until 60 minutes under extracorporeal circulation. 6. We used 1.5mg of protamine to neutralize 100 units of heparin. But smaller dose of protamine may be sufficient for appropriate neutralization.

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A study of symptoms and pathogenesis of hakgil(瘧疾) in the chinese traditional medical literature until chung(淸) dinasty (학질(瘧疾)의 증상(症狀)과 기전(機轉)에 대한 문헌적(文獻的) 고찰(考察) -청대(淸代)까지 중국의서(中國醫書)를 중심(中心)으로-)

  • Ryu, Jeong-A;Park, Chan-Guk
    • Journal of Korean Medical classics
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    • v.12 no.1
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    • pp.168-195
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    • 1999
  • The hakgil is the important disease in the oriental medicine historically. In the preseant time also this disease continually appear all over the world. So purpose of this study is that consider the symptoms and pathogenesis of hakgil(瘧疾) with the point of view of oriental medicine. And in this study, the results are summarized as the followings. 1. The symptos of hakgil(瘧疾). 1) Rigor and heat spasm : The main symptoms of hakgil is the severe and periodical rigor and heat spasm. Generally the rigor first appear and later the heat spasm appear. According to the first and last, severe and weak, the hakgil is classified to hanhak(寒瘧), onhak(溫瘧), danhak(癉瘧), binhak(牝瘧). 2) The regulation of the time of spasm : The spasm occour in the same time daily or one time in two days, three days or several days. And the spasm time is regulary in day or night. 3) The term between the spasm and next one become later or faster. It can be decided that the becoming worse and better in the disease with the signs. 4) The seasonal property Generally the hakgil appear in summer and early autumn. 5) The other kind of hakgil there are five-organ hakgil(五臟瘧), six-kyung hakgil(六經瘧), janghak(瘴瘧), kuihak(鬼瘧), six-gi hakgil(六氣瘧), damhak(痰瘧), sikhak(食瘧), and so on. 6) The pulse condition of the hakgil is chiefly hyun(弦). 2. The pathogenesis of the hakgil 1) The cause of the hakgil The causes of the hakgil first are the seo(暑) or heat(熱) that make the problem in the cycle of five phases(五行). In the consequence, il open the hole of skin so that the pathogenic factors easily invade the humanbody and at the same time the pathogenic factor in the inside easily come out, that make the spasm. In the second time the pathogenic factor of yin(陰) - wind(風), cold(寒), water(水) invade through the opened skin to combine with the factor in the inside. Such condition make the hakgil and the accessory spasm. 2)The pathogenesis of hakgil(瘧疾) (1) The rigor and heat spasm of hakgil(瘧疾) appear because in summer the human body don't accomplish a task of summer because of hot weather or heat, so in autumn the ki(氣) of human body separate into yin(陰) and yang(陽), and the skin of human body is weaken so the saki(邪氣: pathogenic factors) is easily come into the human body. At this time the circulation of ki(氣) is obstructed, so the jungki(精氣: vital substance) apply to straighten the circulation of ki(氣), if the jungki(精氣: vital substance) help the yin(陰) the rigor spasm appear in the opposit direction the jungki(精氣: vital substance) help the yang(陽) the heat spasm appear. (2) The period of circulation of ki(氣) and jungki(精氣: vital substance) is one day, so the general period of spasm of hakgil(瘧疾) is one day, But if the saki(邪氣: pathogenic factors) come into the human body deeply, the jungki(精氣: vital substance) cannot apply 10 straighten the circulation of ki(氣) every day so the period of spasm become longer.

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Resource Circulation Plan using Material Flow Analysis of Waste Metals of Cobalt and Palladium (코발트와 팔라듐 폐금속자원의 흐름분석을 통한 자원순환 활성화 방안)

  • Lee, Hi Sun;Lee, Jeongmin;Yi, Sora
    • Resources Recycling
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    • v.27 no.1
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    • pp.14-21
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    • 2018
  • The rapid increase in the consumption of products that contain rare metals has highlighted the importance of recycling and recovering resources from these products when they enter the waste stream. Among various metal resources that can be recovered, this study analyzes the waste streams of cobalt and palladium to determine how their waste resource circulation can be improved at each stage of the waste stream. The findings of this study point to improvements and strategies that can be made at individual stages. First, at the discharge/import stage, the implementation of tariff quotas for specific recycled metal resources is suggested to allow the systemic categorization of waste metals as resources. At the collection/discarding stage, a major problem is the instability in the supply of scrap metals, which may be better managed by changing the bidding process for the scrap metals. At the pretreatment stage, possible areas for improvement are uncovered concerning technical areas, such as technological development and improving the efficiency of material recycling, as well as policy-wise, for instance, expanding the regulation for manufacturers to produce products that are designed to facilitate resource recovery, increasing incentive for closed recycling, and refining the guidelines and standards for recycling. At the resource recovery stage, as the waste metal recycling industry consists of businesses that vary in size, policies to promote cooperation and coexistence between large and smaller enterprises will benefit the industry in the long-run. Lastly, at the product production/export stage, a tariff on exporting waste resources that contain cobalt and palladium will help control the amount of waste metals that are shipped abroad.

On the Change of Flood and Drought Occurrence Frequency due to Global Warming : 1. Change of Daily Rainfall Depth Distribution due to Different Monthly/Yearly Rainfall Depth (지구온난화에 따른 홍수 및 가뭄 발생빈도의 변화와 관련하여 : 1. 연/월강수량의 변화에 따른 일강수량 분포의 변화분석)

  • Yun, Yong-Nam;Yu, Cheon-Sang;Lee, Jae-Su;An, Jae-Hyeon
    • Journal of Korea Water Resources Association
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    • v.32 no.6
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    • pp.617-625
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    • 1999
  • Global warming has begun since the industrial revolution and it is getting worse recently. Even though the increase of greenhouse gases such as $CO_2$ is thought to be the main cause for global warming, its impact on global climate has not been revealed clearly in rather quantitative manners. However, researches using General Circulation Models(GCMs) has shown the accumulation of greenhouse gases increases the global mean temperature, which in turn impacts on the global water circulation pattern. This changes in global water circulation pattern result in abnormal and more frequent meteorological events such as severe floods and droughts, generally more severe than the normal ones, which are now common around the world and is referred as a indirect proof of global warming. Korean peninsula also cannot be an exception and have had several extremes recently. The main objective of this research is to analyze the impact of global warming on the change of flood and drought frequency. Based on the assumption that now is a point in a continuously changing climate due to global warming, we analyzed the observed daily rainfall data to find out how the increase of annual rainfall amount affects the distribution of daily rainfall. Obviously, the more the annual rainfall depth, the more frequency of much daily rainfall, and vice versa. However, the analysis of the 17 points data of Keum river basin in Korea shows that especially the number of days of under 10mm or over 50mm daily rainfall depth is highly correlated with the amount of annual rainfall depth, not the number of dry days with their correlation coefficients quite high around 0.8 to 0.9.

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Warm Water Circulation and its Origin by Sea Level Fluctuation and Bottom Topography (해수면변화와 해저지형에 의한 난류수의 순환과 그 기원)

  • PARK Ig-Chan;OH Im Sang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.5
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    • pp.677-697
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    • 1995
  • The analysis of long- period sea level variations with tidal record data around Korea, Japan, and Russia shows that about half of the variations are due to atmospheric influences. The sea level variation by water movements is the largest in the coasts along the Tsushima Current, and becomes smaller in the distant areas. It suggests that the sea level varications are related with the Tsushima Current. The effect of sea level variations to ocean circulation has been studied with a numerical model allowing barotropic sea level fluctuations, like the result with GCM (Semtner) model by Pang et al.(1993), the present model also shows that waters basically flow along isobaths over the last China Sea after geostyophic adjustment around Taiwan. However, barotropic sea level fluctuation makes the basic circulation in the Yellow Sea, which waters flow into the central Yellow Sea and out along the west coast of the Korean Peninsula. Besides this, barotropic sea level fluctuation makes long period waves over the shelf area as the Kuroshio varies. By the waves, the basic circulation in the Yellow Sea is disturbed, so that the flow pattern of oppositely flowing into the Yellow Sea along the west roast of the Korean Peninsula appears. In the Yellow Sea circulation, it seems that northwest winds strengthen the basic circulat ion In winter, and southeast winds strengthen the disturbed circulation in summer. Another point appeared by the long period wave is that the Tsushima Current possibly originates in different areas. There have been two opposing argues on the area in which the Tsushima Current originates the southwest sea of Kyushu Island and the adjacent sea of Taiwan. Through this study, we found that both of them seem to be important areas for the origin of the Tsushima Current, and one of them is possibly strengthened by long period waves. The long period waves given by the variation of the Kuroshio Current in the adjacent sea of Taiwan propagate to the Korea Strait as forced waves. The wave continuously propagates to the last Sea through the eastern channel, but reflects in the western channel due to bottom topography. The reflected waves propagate southwestward along the last China Sea as free waves and determine the sea level variations with forced waves.

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Efficiency and Behavior of P32-labeled Phosphorus in Camellia japonica L in Blooming Stage (개화기(開花期)에 들어선 동백나무(Camellia japonica L)의 P32 표식(標識) 인산(燐酸)의 행동(行動)과 효율(効率)에 대(對)하여)

  • Kim, Jong Man;Kim, Yong Kwan
    • Journal of Korean Society of Forest Science
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    • v.9 no.1
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    • pp.75-80
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    • 1969
  • Traced observations were carried out for efficiency and behavior of Camellia japonica L in bloom stage by employing $p^{32}$-labeled phosphorus, growing in Southern Korea, and the results were as follows. 1) The percentage of phosphorus contained in flowerbud was double the value of the other parts, and the root and the stem were the same patterns in the course of the times. 2) The circulation of the phosphorus in the plant seems to occur within 24 hours, the proportions of the phosphorus at the growing-point and flowerbud had a half of the total phosphorus contained in other parts. 3) Specific activities of each part were notable on the growing-point where, after 24 hours it took more than 80 percent. On the other hand, the efficiency rate which derived from the phosphorus was shown as about 19 percents.

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