• 한국융합학회논문지
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• 제11권2호
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• pp.309-314
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• 2020

본 연구는 전통 식재료로 많이 사용되는 10종의 약용식물 및 채소류 추출물과 10종 혼합추출물의 항산화 효과를 살펴보기 위해서 디자인되었다. 세포 생존률은 MTS assay로, 항산화효과는 DPPH free radical 소거능, Riboflavin 유래 Superoxide 억제활성(SQA)에 의한 항산화활성을 측정하였다. 그 결과 세포생존률은 10종 약용식물 및 채소류 추출물과 10종 혼합추출물 모두 0.5mg/mL에서 대조군과 유사한 생존율을 보였다. DPPH free radical 소거능은 Cinnamomum cassia Blume, Eugenia caryophyllata Thunb. Arctium lappa, Total extract가 우수하였고, Riboflavin 유래 Superoxide 억제활성(SQA)은 Cinnamomum cassia Blume, Arctium lappa, Prunus mume Sieb. et Zucc., 우수하였으나, Total extract가 가장 우수한 항산화 효과를 보였다. 전통식재료로 사용되는 약용식물 및 채소 추출물과 혼합추출물의 항산화 효과를 비교한 결과, 단독으로 사용하는 것보다 혼합물로 사용시 항산화능이 증가되었다. 이는 항산화 기능성 소재로 활용이 가능한 것으로 사료되며, 향후 항산화 소재 개발 시 가치가 있을 것으로 사료된다.

• 한방비만학회지
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• 제15권2호
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• pp.131-136
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• 2015

Objectives: The prevalence of obesity and metabolic syndrome is increasing worldwide. Regulation of cellular energy metabolis has the potential to be manipulated therapeutically to serve as a target for obesity and insulin resistance. Skeletal muscle is regarded as a target for regulation of energy metabolism and insulin resistance. In this study, the authors investigated the regulatory effect of (Cinnamomum cassia Blume, CCB), Aconitum carmichaeli Debx (ACD), and Benth (Pueraria lobata Benth, PLB) on energy and glucose metabolism in C2C12 myotubes. Methods: The water extracts of CCB, ACD, and PLB (0.5 mg/ml) were treated in differentiated C2C12 myotubes. The expressions of adenosine monophosphate-activated protein kinase (AMPK) and phosphorylation AMPK were detected with western blotting. Glucose metabolism was investigated with glucose uptake assay and glucose consumption assay, total adenosine triphosphate (ATP) content was also analyzed. Results: CCB, ACD, and PLB activated the phophorylation of AMPK, they also increased the glucose metabolism and total ATP contents in C2C12 myotubes. Conclusions: This study suggests that CCB, ACD, and PLB have the potential to increase energy and glucose metabolism in skeletal muscle.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1176-1181
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• 2004

The cytotoxic activities of Cinnamomum cassia (Blume) bark-derived materials toward six human HeLa epithelioid cervix, A549 lung, SK-OV-3 ovarian, SK-MEL-2 melanoma, XF-498 central nerve system, and HCT-15 colon tumor cell lines were evaluated by using sulforhodamine B assay and compared to those of the anticancer agents, cisplatin and mitomycin C. The biologically active constituent of the Cinnamomum bark was characterized as trans­cinnamaldehyde by spectroscopic analysis. The cytotoxic activity of cinnamaldehyde against HeLa, SK-MEL-2, and HCT -15 cell lines was comparable to that of cisplatin and mitomycin C. The compound showed lower activity against A549, SK-OV-3, and XF-498 cell lines than the anticancer agents. Eugenol exhibited moderate activity against SK-OV­3, XF-498, and HCT-15 tumor cells, and trans-cinnamic acid, cinnamyl alcohol, $\alpha-pinene,\;and\;\beta-pinene$ showed little or no activity against model tumor cells. Cinnamaldehyde was not mutagenic against four strains (TA 98, TA 100, TA 1535, and TA 1537) of Salmonella typhimurium (Castel and Chalm). These results indicate at least one pharmacological action of C. cassia.

• 한국임상수의학회지
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• 제30권3호
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• pp.159-165
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• 2013

본 연구는 내측반월판 절제와 전십자인대 단열로 유발된 랫드 골관절염 모델을 이용하여 계피 (Cinnamomum cassia Blume, 육계(肉桂)) 추출물의 연골손상 방지에 대한 효과를 평가하였다. 골관절염유발 랫드 48마리를 군당 8마리씩 6군으로 음성 대조군과 골관절염 대조군, 체중당 diclofenac 2 mg 투여군, 계피 추출물 25 mg과 50 mg, 100 mg 투여군으로 각각 분류하여 수술 1주 후부터 12주동안 투여하였다. 연골 소실과 관절의 불안정성은 계피 추출물과 diclofenac 투여군이 골관절염 대조군과 비교할 때 유의하게 감소하였다(p < 0.05). 병리조직학적 평가에서 연골의 퇴행은 계피 추출물 투여량에 따라 용량 의존적으로 개선됨이 확인되었다(p < 0.01). 계피 추출물 투여군에서 관절구조 퇴행성 변화의 감소와 Safranin-O 염색 정도의 용량 의존적인 증가를 보여 연골 퇴행이 억제됨을 확인하였다. Diclofenac이 골관절염 진행에 있어 활성화된 caspase-3와 절단된 poly(ADP-ribose) 중합효소에 유도된 세포자멸사 표지율 증가에 별 다른 영향을 주지 않았지만 계피 추출물 투여군에서는 이들 세포자멸 표지자에 대한 반응세포는 유의하게 감소되었다(p < 0.05). 그러나, diclofenac과 계피 추출물 투여군은 5-bromo-2-deoxyuridine의 섭취에는 영향을 주지 않았다. 이러한 결과에서 계피추출물이 항염활성과 항세포 자멸 활성을 통해 관절연골에 보호 효과가 있음을 보였다.

• 한국식품조리과학회:학술대회논문집
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• 한국식품조리과학회 2005년도 춘계학술대회
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• pp.151-151
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• 2005

• 사상체질의학회지
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• 제23권2호
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• pp.164-173
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• 2011

1. Objectives: The purpose of this study is to understand the origin, the progressive changes and the constructive principles of Packhaoleejung-tang (白何烏理中湯) 2. Methods: Packhaoleejung-tang and other related prescriptions were analyzed in terms of their pathological indications, based on "Donguisusebowon Chobongwon (東醫壽世保元 草本卷)", "Donguisusebowon Gabobon (東醫壽世保元 甲午本)", "Donguisusebowon Sinchukbon (東醫壽世保元 辛丑本)", "Dongmuyugo (東武遺稿)", "Shanghanlun (傷寒論)". 3. Results and Conclusions: (1) The origin of Packhaoleejung-tang is leejung-tang from "Shanghanlun (傷寒論)". Packhaoleejung-tang inherited some of the principles from "Shanghanlun (傷寒論)" Insamkeiji-tang (2) "Gabobon(甲午本)"'s one prescription Hyangsaleejung-tang emphasizes the effect of lossen up by using Pogostemonis Herb (藿香),Amomi Fructus(砂仁). "Gabobon(甲午本)"'s another prescription Packhaoleejungtang emphasizes the effect of warm up stomach by using Cinnamomum cassia Blume (桂枝), Allium sativum Linne (獨頭蒜), Cynanchum wilfordii(白何首烏). "Sinchukbon(辛丑本)"'s prescription Packhaobujaleejungtang emphasizes the effect of warm up stomach by using Aconitum carmichaeli(附子) (3) The Packhaoleejung-tang is composed of 7 herbs. Atractlodis Rhizoma White(白朮), Glycyrrhizae Radix(甘草), Paeoniae Radix Alba(白芍藥) support Soeumin's spleen root (脾元), and Zingiberis Rhizoma(乾薑),Cinnamomum cassia Blume (桂枝, Cynanchum wilfordii(白何首烏) warm up stomach. Citri Pericarpium(陳皮) circulate Qi and encourage the descension of Yin.

• 한방비만학회지
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• 제17권2호
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• pp.119-126
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• 2017

Objectives: This study investigated the effects of the mixed herbal extract from Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata Benth, and Aconitum carmichaeli Debx (CAPA) on obesity and glucose tolerance in obese mice. Methods: Animals were divided in 6 groups, normal diet, high fat diet (HFD), HFD with CAPA 100 mg/kg (CAPA 100), CAPA 300 mg/kg (CAPA 300), and metformin 200 mg/kg or lorcaserin 10 mg/kg as positive controls, and treated for 16 weeks. Body weight, food intake, fasting blood glucose, and body temperature were checked every week and then organs, blood serums were collected after treatment. The oral glucose tolerance test was also carried out after treatment. Results: Compared to HFD, CAPA extract treated mice showed significant decreases in body weight, adipose tissue weight, lipid accumulations in liver and serum lipid levels without a reduction of food intake. And fasting glucose and glucose tolerance were all improved in the CAPA treated mice. Conclusions: Our results suggest that CAPA extract can prevent diet induced obesity and glucose intolerance without a reduction of energy intake in obese mice.

• 한국식품영양과학회지
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• 제26권2호
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• pp.248-253
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• 1997

계지에서 분리한 보체계 활성화 다당의 보체 활성화양식과 다당의 당쇄결합에 관하여 검토하였다. 계지 보체계 활성화 다당획분들(CC-IIIa, CC-IIIb, CC-IIIc)들은 정상인의 혈청중에 존재하는 주요 보체성분인 C3를 활성화하여 C3a와 C3b 보체 산물을 생성 시켰는데 이 과정은 anti-human C3를 이용한 면역전기영동을 실시하여 확인하였다. $Ca^{++}$ 유무시의 보체계 활성을 측정한 결과 계지에서 분리된 CC-IIIa-3는 고전적 경로(classical pathway)와 부경로(alternative pathway)를 동시에 활성화시킴을 확인하였다. CC-IIIa-3을 메틸화하여 GC/MS로 분석한 결과 비환원 말단의 galactopyranose와 glucopyranose가 확인되었고 1,2-linked rhamnopyranose, 1,6-linked mannopyranose, 1,3-linked glucopyranose, 1,6-linked galactopyranose 등이 확인되었다.

• Archives of Pharmacal Research
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• 제21권2호
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• pp.147-152
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• 1998

Cinnamaldehydes and related compounds were synthesized from various cinnamic acids based on the $2^{I}$-hydroxycinnamaidehyde isolated from the bark of Cinnamomum cassia Blume. The cytotoxicity to human solid tumor cells such as A549, SK-OV-3, SK-MEL-2, XF498 and HCT15 were measured. Cinnamic acid, cinnamates and cinnamyl alcohols did not show any cytotoxicity against the human tumor cells. Cinnamaldehydes and realted compounds were resistant to A549 cell line up to 15 .mu.g/ml. In contrast, HCT15 and SK-MEL-2 cells were much sensitive to these cinnamaidehyde analogues which showed $ED{50} values 0.63-8.1{\mu}g/ml.$Cytotoxicity of the saturated aldehydes was much weak compared to their unsaturated aldehydes. From these studies, it was found that the key functional group of the cinnamaldehyde-related compounds in the antitumor activity is the propenal group.p.

• Applied Biological Chemistry
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• 제41권4호
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• pp.218-221
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• 1998

Various cinnamaldehyde derivatives were synthesized and their inhibition activity $(pI_{50})$ of farnesyl protein transferase (FPTase) was measured to examine the structure-activity relationships (SAR) on the basis that FPTase was inhibited by ortho-hydroxycinnamaldehyde derived from extracts of the bark of Cinnamomum cassia Blume. The ortho-substituents on the phenyl backbone of cinnamaldehyde showed higher activity than those with meta- and para-substituents, and the side chain required unsaturated aldehyde. In particular, 2-chlorocinnamaldehyde, 5 showed the highest inhibition activity on the FPTase among them and its inhibition activity $(pI_{50})$ value was 4.45.