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Antioxidant Effect of Traditional food Ingredient

전통 식재료의 항산화 효과

  • Received : 2019.12.16
  • Accepted : 2020.02.20
  • Published : 2020.02.28

Abstract

This study was designed to investigate the antioxidant effects of 10 kinds of medicinal plants and vegetable extracts and total extracts. The cytotoxicity was measured by MTS assay, and the antioxidant activity was measured by DPPH free radical scavenging activity and Riboflavin-derived superoxide inhibitory activity (SQA). As a result, cytotoxicity was safe for all 10 medicinal plants, vegetable extracts and total extracts. DPPH free radical scavenging ability was observed in Cinnamomum cassia Blume, Eugenia caryophyllata Thunb. Arctium lappa, Total extract was excellent, and Riboflavin-derived superoxide inhibitory activity (SQA) was found in Cinnamomum cassia Blume, Arctium lappa, Prunus mume Sieb. et Zucc., Excellent, but total extract showed the best antioxidant effect. As a result of comparing the antioxidant effects of medicinal plants and vegetables using traditional ingredients, the antioxidant activity was increased when used as a mixture than when used alone. It is considered that it can be used as an antioxidant functional material, and it is expected to be of value when developing antioxidant material in the future.

본 연구는 전통 식재료로 많이 사용되는 10종의 약용식물 및 채소류 추출물과 10종 혼합추출물의 항산화 효과를 살펴보기 위해서 디자인되었다. 세포 생존률은 MTS assay로, 항산화효과는 DPPH free radical 소거능, Riboflavin 유래 Superoxide 억제활성(SQA)에 의한 항산화활성을 측정하였다. 그 결과 세포생존률은 10종 약용식물 및 채소류 추출물과 10종 혼합추출물 모두 0.5mg/mL에서 대조군과 유사한 생존율을 보였다. DPPH free radical 소거능은 Cinnamomum cassia Blume, Eugenia caryophyllata Thunb. Arctium lappa, Total extract가 우수하였고, Riboflavin 유래 Superoxide 억제활성(SQA)은 Cinnamomum cassia Blume, Arctium lappa, Prunus mume Sieb. et Zucc., 우수하였으나, Total extract가 가장 우수한 항산화 효과를 보였다. 전통식재료로 사용되는 약용식물 및 채소 추출물과 혼합추출물의 항산화 효과를 비교한 결과, 단독으로 사용하는 것보다 혼합물로 사용시 항산화능이 증가되었다. 이는 항산화 기능성 소재로 활용이 가능한 것으로 사료되며, 향후 항산화 소재 개발 시 가치가 있을 것으로 사료된다.

Keywords

References

  1. Y. J. Cho, I. S. Ju, O. J. Kwon, S. S. Chun, B. J. An & J. H. Kim. (2008). Biological and antimicrobial activity of Portulaca oleracea. J Korean Soc Appl Biol Chem, 51, 49-54.
  2. P. Albertazzi, S. A. Steel, E. Clifford & M.Bottazzi. (2002). Attitudes towards and use of dietary supplementation in a sample of postmenopausal women. Climacteric, 5(4), 374.
  3. A. L. Branen. (1975). Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc, 52, 59-63. https://doi.org/10.1007/BF02901825
  4. S. Y. Choe & K. H. Yang. (1982). Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) Korean. J Food Sci Technol, 12, 283-288.
  5. J. S. Shim, S. D. Kim, T. S. Kim & K. Kim. (2005). Biological activities of flavonoid glycosides isolated from Angelica keiskei. Korean J Food Sci Techol, 37, 78-83.
  6. Y. J. Cho, I. S. Ju, S. S. Chun, B. J. An, J. H. Kim, M. W. Kim & O. J. Kwon. (2008). Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr, 37, 276-281. https://doi.org/10.3746/JKFN.2008.37.3.276
  7. J. Kedziora & G. Bartosz. (1988). Down's syndrome: a pathway involving the lack of blance of reactiveoxygen species. Free Radic. Biol. Med., 4(5), 317-319. https://doi.org/10.1016/0891-5849(88)90052-4
  8. M. T. Huang, C. T. Ho & C. Lee. (1992). Phenolic compounds in food and their effects on health (II), antioxidants and cancer prevention. ACS symp series, 507, 54-71.
  9. M. A. Kang, M. B. Kim, J. H. Kim, Y. H. Ko & S. B. Lim. (2010). Integral antioxidative capacity and antimicrobial activity of pressurized liquid extracts from 40 selected plant species. J Korean Soc Food Sci Nutr, 39, 1249-1256. https://doi.org/10.3746/JKFN.2010.39.9.1249
  10. J. H. Lee & J. S. Park. (2019). Antibacterial effect of traditional food ingredients for healthcare on Helicobacter pylori. Technology and Health Care, 27, 509-518.
  11. A. Desai, T. Vyas & M. Amiji. (2008). Cyroroxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J. Pharm Sci., 97, 2745-2751. https://doi.org/10.1002/jps.21182
  12. D. G. Kim & D. Y. Yoo. (2012). The Effects of Cinnamom umloureirii on Osteoblast in Murine Rat Calvarial Cells. J. Oriental Obstetrics & Gynecology, 25(3), 61-70.
  13. J. Lin, L. Xu & J. Liu. (2006). HPLC fingerprint of Ramulus Cinnamomi. Chinese Traditional Patent Medicine, 28(2), 169-171. https://doi.org/10.3969/j.issn.1001-1528.2006.02.004
  14. A. K. Singh, S. S. Dhamanigi & M. A. sad. (2009). Anti-stress activity of hydroalcoholic extract of Eugenia caryophyllus buds(clove). Indian J. Pharmacol., 41(1), 28-31. https://doi.org/10.4103/0253-7613.48889
  15. M. Ito, K. Murakami & M. Yoshino. (2005). Antioxidant action of eugenol compounds: role of metal ion in the inhibition of lipid peroxidation. Food Chem. Toxicol., 43(3), 461-466. https://doi.org/10.1016/j.fct.2004.11.019
  16. G. Kaur, M. Athar & M. Alam. (2010). Eugenol precludes cutaneous chemical carcinogenesis in mouse by preventing oxidative stress and inflammation and by inducing apoptosis. Mol. Carcinog. 49(3), 290-301. https://doi.org/10.1002/mc.20601
  17. M. S. Lee. (2011). Antioxidative and antimutagenic effects of Arctium lappa ethanol extract. Korean J. Food & Nutr. 24, 713-719. https://doi.org/10.9799/ksfan.2011.24.4.713
  18. F. A. Chen, A. B. Wu & C. Y. Chen. (2004). The influence of different treatment on the free radical scavenging activity of burdock and variations of its active components. Food. Chem, 86, 479-484. https://doi.org/10.1016/j.foodchem.2003.09.020
  19. R. Ferracane, G. Graziani, M. Gallo, V. Fogliano & A. Ritieni. (2010). Metabolic profile of the bioactive compounds of burdock (Arctium lappa ) seeds, roots and leaves. J. Pharm. Biomed. Ana, 51, 399-404. https://doi.org/10.1016/j.jpba.2009.03.018
  20. Y. D. Kim & K. S. Seo. (2007). Prunus mume's bioactivity and effect of oriental medicine. Food Preservation and Processing Industry, 6, 31-38.
  21. C. E. Park. (2006). Inhibition of helicobacter pylori urease activity by mume fructus, Ph.D thesis. Kyung Hee University. Seoul, Korea.