• Title/Summary/Keyword: Ciliates

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Protozoa population and carbohydrate fermentation in sheep fed diet with different plant additives

  • Majewska, Malgorzata P.;Miltko, Renata;Belzecki, Grzegorz;Kedzierska, Aneta;Kowalik, Barbara
    • Animal Bioscience
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    • v.34 no.7
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    • pp.1146-1156
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    • 2021
  • Objective: The aim of the study was to compare the effect of two plant additives, rich in polyphenolic compounds, supplemented to sheep diets on microorganisms and carbohydrate fermentation in rumen. Methods: In the experiment, 6 ewes of the Polish Mountain breed were fitted with ruminal cannulas. Sheep were divided into three feeding groups. The study was performed in a cross-over design of two animals in each group, with three experimental periods (n = 6 per each group). The animals were fed a control diet (CON) or additionally received 3 g of dry and milled lingonberry leaves (VVI) or oak bark (QUE). Additionally, plant material was analyzed for tannins concentration. Results: Regardless of sampling time, QUE diet increased the number of total protozoa, as well as Entodinium spp., Diplodinium spp. and Isotrichidae family, while decreased bacterial mass. In turn, a reduced number of Diplodinium spp. and increased Ophryoscolex spp. population were noted in VVI fed sheep. During whole sampling time (0, 2, 4, and 8 h), the number of protozoa in ruminal fluid of QUE sheep was gradually reduced as opposed to animals receiving CON and VVI diet, where rapid shifts in the protozoa number were observed. Moreover, supplementing sheep with QUE diet increased molar proportions of butyrate and isoacids in ruminal fluid. Unfortunately, none of the tested additives affected gas production. Conclusion: The addition of VVI or QUE in a small dose to sheep diets differently affected rumen microorganisms and fermentation parameters, probably because of various contribution of catechins in tested plant materials. However, it is stated that QUE diet seems to create more favorable conditions for growth and development of ciliates. Nonetheless, the results of the present study showed that VVI and QUE additives could serve as potential natural modulators of microorganism populations and, consequently, carbohydrate digestion in ruminants.

New Records of Two Arcuospathidium Subspecies (Ciliophora: Haptoria: Arcuospathidiidae) from Korea

  • Jang, Seok Won;Nam, Seung Won;Shazib, Shahed Uddin Ahmed;Shin, Mann Kyoon
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.4
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    • pp.226-237
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    • 2022
  • Arcuospathidium is a haptorian ciliate genus composed of 18 species, and only one species has been reported in Korea. Here, we identify two unrecorded Arcuospathidium subspecies by morphological observation of both living and protargol-impregnated specimens with the small subunit ribosomal RNA (18S rRNA) gene sequence. These subspecies, Arcuospathidium cultriforme cultriforme (Penard, 1922) Foissner, 1984 and A. cultriforme scalpriforme (Kahl, 1930) Foissner, 2003, were isolated from various terrestrial habitats in July and August 2013, respectivley. Arcuospathidium cultriforme cultriforme is similar to A. cultriforme scalpriforme by a knife-shaped body, a twisted-shaped macronucleus, number of dorsal brushes, position of dorsal brushes, and shape of macronucleus but former mainly differs from the body length to oral bulge length ratio (27-38% vs. 41-53%), extrusome (one types vs. three types), cyst shape (roughly faceted wall vs. smooth surface and thin wall) and number of somatic kinety rows(18-30 vs. 30-44). Additionally, we analyzed the 18S rRNA gene sequences of two A. cultriforme subspecies and compared them with the sequences from GenBank to confirm their identification at the molecular level. As the results of genetic analysis, the 18S rRNA gene sequence of the Korean A. cultriforme cultriforme population is most similar to that of Austrian population. Also, the sequence of the Korean A. cultriforme scalpriforme population is most similar to that of another population with some nucleotide differences.

The Outbreak of Red Tides in the Coastal Waters off Kohung, Chonnam, Korea 3. The Temporal and Spatial Variations in the Heterotrophic Dinoflagellates and Ciliates in 1997 (전남 고흥 해역의 유해성 적조의 발생연구 3. 1997년도 종속영향성 와편모류와 섬모류의 시공간적 변화)

  • Jeong, Hae-Jin;Park, Jong-Kyu;Kim, Jae-Seong;Kim, Seong-Taek;Yoon, Joo-Eh;Kim, Su-Kyeong;Park, Yong-Min
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.1
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    • pp.37-46
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    • 2000
  • We investigated the temporal and spatial variations in heterotrophic dinoflagellates (hereafter HTD) and ciliates from June to September 1997 in the waters off Kohung, Korea where red tides dominated by harmful dinoflagellates had occurred from August to October since 1995. We took water samples five times from 5-7 depths at 3 stations in this study period. A total of 17 HTD species were present and of these species in the genus Protoperidinium were 11. The species number of tintinnids (hereafter TIN) present totalled 15 and several naked ciliate (hereafter NC) species were observed. The species numbers of HTD and TIN rapidly increased between August 1st and 21st and then reached to the maximum numbers of 13 and 10, respectively, on August 27 when red tides dominated by Gyrodinium impudicum were first observed in the study area. However the species numbers drastically decreased on September 22. The maximum densities of HTD, TIN, and NC were 45, 39, 57 cells $ml^{-1}$, respectively. ADAS, calculated by averaging the densities of a certain species in the all samples collected from all depths and stations at a sampling period, most increased between August 1st and 21st and then reached to the maximum density of f cells $ml^{-1}$ on August 27 for HTD, while did between August 21st and 27th and up to 7 cells $ml^{-1}$ for TIN. Unlike ADAS of HTD and TIN, that of NC did not change much with the maximum of 8 cells $ml^{-1}$ on August 27th. The pattern of the temperal variation in the species number and ADAS of HTD was similar to that of diatoms and the distributions of Protoperidinium spp. and diatoms had a strong positive correlation. This evidence suggests that HTD, in particular Protoperidinium spp. be a grazer on diatom. In general, the densities of HTD, TIN, and NC decreased with going to stations located in the outer bay. Therefore, the availability of suitable prey and distance from the coastal line might be responsible for the distribution of HTD, TIN, and NC. The results of the present study provide a basis for further experiments for the feeding by dominant HTD, TIN, and NC on dominant phytoplankton including red tide species and for understanding food webs in the planktonic community before, during, and after the red tide outbreak.

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A Field Application Feasibility of Biologically Derived Substances (Naphthoquinone Derivate: NQ 2-0) for the Mitigation of Harmful Cyanobacterial Blooms (유해 남조류 제어를 위한 생물유래 살조물질 Naphthoquinone 유도체 (NQ 2-0)의 현장 적용 가능성)

  • Joo, Jae-Hyoung;Park, Chong-Sung;Choi, Hye Jeong;Lee, Heon Woo;Han, Myung-Soo
    • Ecology and Resilient Infrastructure
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    • v.4 no.3
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    • pp.130-141
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    • 2017
  • We evaluated the field application feasibility that biologically derived substances (Naphthoquinone derivate: NQ 2-0) can be used for the eco-friendly mitigation of natural harmful cyanobacterial blooms in freshwater. We conducted a 30 ton scale mesocosm experiment to investigate the effects of NQ 2-0 on biotic and abiotic factors in water collected from Gi-heung reservoir. In the mesocosm experiments, the abundance of Microcystis sp. was continuously increased in the control. However, the Microcystis sp. cell density was sharply decreased on the $10^{th}$ day. In the treatment, NQ 2-0 showed the strong and selective algicidal activity toward the target cyanobacteria (Microcystis sp.). Accordingly, the algicidal activity of NQ 2-0 compound increased gradually until $10^{th}$, $15^{th}$ days and algal biomass was decreased to 99.4 and 100 %, respectively. NQ 2-0 compound was not only selective algicidal activity but also the growth of other phytoplankton and increased the Shannon-Wiener diversity index of phytoplankton. In the mesocosm experiments, the dynamics of biotic (bacteria, heterotrophic nanoflagellate, ciliates, zooplankton) and abiotic (water temperature, dissolved oxygen, pH, conductivity, nutrients) factors remained unaffected. These results suggest that NQ 2-0 could be a selective and ecologically safe algicide to mitigate harmful cyanobacterial blooms. In addition, it is believed that NQ 2-0 will play a major role in forming a healthy aquatic ecosystem by facilitating habitat and food supply of aquatic organisms.

Effects of Stocking and Removal of Silver Carp on Plankton Communities in a Shallowhypertrophic Lake (부영양호의 enclosure내에서 어류의 밀도조절이 수질 및 플랑크톤 군집에 미치는 영향)

  • Choi, Min-Kyu;Hwang, Su-Ok;Noriko, Takamura;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.33 no.4 s.92
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    • pp.358-365
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    • 2000
  • To evaluate the influences of stocking and removal of silver carp on plankton communities and physicochemical parameters, fish biomanipulation was conducted in six enclosures constructed on the coast of a hypertrophic lake during the summer of 1997. Of these, three enclosures were established for the fish-stocking experiment; one fishless enclosure used as a control and two enclosures with high and low densities of fish. The other three enclosures for the fish-removal experiment were prepared in an entirely converse process compared to that for fish stocking. The results of randomized intervention analysis (${\alpha}=0.05$) showed significant changes in several physicochemical parameters and plankton communities after fish stocking and removal. Fish stocking decreased water transparency, DO, pH and chi-a (> $40\;{\mu}m$), while increasing the concentration of nitrogen and chi-a (<$2\;{\mu}m$). However, water transparency did not increase by fish removal. Small plankton communities, such as picocyanobacteria, eukaryotic picoplankton and ciliates (<$2\;{\mu}m$) showed significant reciprocal changes after both fish treatments: increase by the presence of fish and decrease by the absence of fish. No count of large sized phytoplankton and zooplankton showed significant change in the presence of fish, while large zooplankton, Diaphanosoma, Cyclopida and nauplii were significantly increased by fish removal. Although the frequent heavy-winds and endless supply of phosphorus from the lake sediment strongly disturbed the fish behavior and enclosure management, the stocking and removal of silver carp showed an obvious reciprocal influences on plankton communities and its physico-chemical parameters in a shallow-hypertrophic lake.

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Immune Response of Olive flounder, Paralichthys olivaceus against Miamiensis avidus (Ciliophora: Scuticociliatida) (스쿠티카충 Miamiensis avidus에 대한 넙치의 면역반응)

  • Jung, Sung-Ju;Kitamura, Shin-Ichi;Aoyana, Masato;Song, Jun-Young;Kim, Byeong-Kwan;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.19 no.2
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    • pp.173-181
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    • 2006
  • Miamiensis avidus is a scuticociliate causing mortality in olive flounder Paralichthys olivaceus. To evaluate immune response of olive flounder against M. avidus, 2.6×106cells/fish of Formalin killed cell (FKC) was intraperitoneally (i.p.) injected, and 2.4 × 106cells/㎖ of sonicated FKC was immersion immunized to 14.9 cm (26.8g) fish. Fish were immunized 2 times with 2 weeks intervals. Antiserum from immunized fish caused agglutination and immobilization of the ciliate. In ELISA test, immunized group exhibited higher titers than control group. In addition, i.p. immunized fish showed higher phagocytic ratio, phahgocytic index and chemotaxic activity. To evaluate in vivo efficacy of FKC on fish, 1.43 × 105cells/fish was i.p.. injected, and 2.2 × 105 cells/㎖ of sonicated cell was immersed into 8.6cm (6.3g) fish. After 2 times immunization with 2 weeks intervals, fish were infected with 2.0 × 104 and 2.0 × 103 cells/ml of live ciliates by immersion. After 3 weeks, cumulative mortality was lower in the i.p. immunized group and mortality was delayed in immersion immunized group. In conclusion, specific immune response of oliver flounder against M. avidus was elevated after immunization and these immune response may prevent and/or delay for the M. avidus infection to olive flounder.

The Community Dynamics of Microbial Food Web during Algal Bloom by Stephanodiscus spp. in Downstream of Nakdong River (낙동강 하류부에서 Stephanodiscus속에 의한 수화 발생시 미생물먹이망 군집 동태)

  • Seo, Jung-Kwan;Lee, Hae-Jin;Chung, Ik-Kyo
    • Korean Journal of Environmental Biology
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    • v.28 no.3
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    • pp.172-178
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    • 2010
  • The bloom of the genus Stephanodiscus was gradually extinguished after 18 April. Counts of bacterial population were increased as the diatom bloom was disappeared. Numbers of the heterotrophic nanoflagellates and ciliates were also increased during the disappearance of the bloom. The densities of the mesozooplankton, the major predator of the diatoms, started to increase in April. However, their growth was suppressed during the bloom period of the diatoms (from January to March). During the bloom period of the diatoms, the monthly average value of the basic productivity amounted up to 11,765.7 mgC $m^{-2}day^{-1}$, which is relatively high value considering the low temperature and light during that period. The growth rate of phytoplankton in March, when the bloom was beginning to be supressed was 0.007. The growth rate of phytoplankton was negative value in April when the decreasing of the algal density was started.

The effect of temperature to scuticociliatida Miamiensis avidus proliferation, and to mortality of infected olive flounder Paralichthys olivaceus (온도가 스쿠티카충 Miamiensis avidus의 증식과 넙치에 감염시 폐사에 미치는 영향)

  • Bae, Min-Ji;Im, Eun-Young;Kim, Heung-Yun;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.22 no.2
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    • pp.97-105
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    • 2009
  • Scuticociliates Miamiensis avidus (syn. Philasterides dicentrarchi) causes high mortality and bad growth in olive flounder Paralichthys olivaceus. Temperature is an important factor not only for growth of pathogens but also for host immune system in poikilothermal animal. In this study, temperature affecting ciliate growth and pathogenicity against olive flounder were examined. Doubling time for the ciliate growth was 61.82 hours at $5{^{\circ}C}$, 26.32 hours at $10{^{\circ}C}$, 21.14 hours at $15{^{\circ}C}$, 16.86 hours $20{^{\circ}C}$ and 16.21 hours at $25{^{\circ}C}$. Maximum ciliate numbers were similar at $10-20{^{\circ}C}$ at the range of $1.54-1.75{\times}10^{5}$/ ml. Duplicated intraperitoneal injections were conducted with the ciliates by the concentrations of $1{\times}10^{2}$, $1{\times}10^{6}$, $1{\times}10^{4}$and $1{\times}10^{5}$/ fish (average 8.34 cm, 4.33 g) then kept at $10{^{\circ}C}$, $15{^{\circ}C}$ and $20{^{\circ}C}$. Cumulative mortality was low at $10{^{\circ}C}$ and the mortality was increasing at higher water temperatures. In addition, cumulative mortality was higher at higher dose of infections. In conclusion, Scuticocilite M. avidus grew well at higher temperature (at $5{^{\circ}C}$, $10{^{\circ}C}$, $15{^{\circ}C}$ and $25{^{\circ}C}$) in vitro, and olive flounder mortality due to M. avidus was highly water temperature and dose dependent. The results of this study suggest that water temperature control may one of the essential factor to reduce mortality due to M. avidus infection.

Effect of the hatchery larval sieving on the larval growth, scuticociliate occurrence, and ensuing spat growth of Patinopecten yessoensis (참가리비, Patinopecten yessoensis 인공종묘 생산시 환수가 유생에 미치는 영향-유생성장, 스쿠티카충 발생, 치패성장의 관점)

  • Jo, Q-Tae;Kim, Su-Kyoung;Lee, Chae-Sung;Lee, Jin-Ho;Park, Mi-Seon;Moon, Tae-Suk
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.303-311
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    • 2010
  • Our previous finding summarizing that larval sieving process is inevitable but triggers the outbreak of scutica-like ciliates (SLCs) in the seed production of Patinopecten yessoensis urged further study to determine best suggestable sieving interval in an agreeable range of water quality. In the mass seed production of the scallop, SLC outbreak was closely related to the larval sieving in which larvae were drained on the basis of every 3-day (5T), 5-day (3T), 7-day (2T), or 9-day (1T) from culture tanks onto a mesh screen and placed back into new water in cleaned tanks. The larval performance of growth and survival was clearly dependent on the sieving intervals. It was in order of 3T, 5T, 2T, and 1T for both of growth and survival and in reverse order for SLC infection frequency, confirming that larval sieving is necessary but damageable if it overwhelms the larval resistance. Interestingly, the larval damages by the sieving persisted to their ensuing spat life in terms of nursery growth, survival, and abnormality.

Temporal Variations and Species Composition of Planktonic Tintinnids (Protist, Ciliophora) in the Korea Strait (대한해협에서 유종섬모충류의 종조성과 시간적 변동)

  • Han, Gook-Bo;Lee, Won-Je
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.15 no.4
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    • pp.176-183
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    • 2010
  • In order to understand the temporal variations and species composition of tintinnids, at two stations of the Korea Strait, this study was carried out from January to December 2007 (n=17). Fifty-one species from 22 genera were encountered: 27 neritic species (9 genera) and 24 oceanic species (14 genera). The species composition was different between two stations, and neritic species frequently appeared at St. 1, while oceanic species more frequently appeared at St. 2. According to the frequency in appearance of species, the most dominant species were neritic species such as Stenosemella nivalis and S. pacifica, that appeared in all seasons. Also, Acanthostomella norvegica, Amphorellopsis acuta, Epilpocyloides ralumensis, Protorhabdonella curta and other many oceanic species appeared intensively during the low salinity (${\leqq}33\;psu$) and high water temperature (${\geqq}24^{\circ}C$) period (July~October). The abundance of tintinnids ranged from 0 to 1,845 cells $L^{-1}$. It was higher in the seasons (summer and autumn) of low salinity and high water temperature than in other seasons. The abundance was relatively higher at St. 1 than at St. 2. On the basis of correlation and multiple regression analyses, the tintinnid abundance was not correlated with chi-a, but significantly correlated with water temperature and salinity, suggesting to be the primary abiotic factors influencing the abundance. This study suggests that the complex hydrographic conditions of the study area might influence the temporal distribution and species composition of tintinnids.