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Growth and Bioactive Compound Contents of Various Sprouts Cultivated under Dark and Light Conditions (광 유무에 따른 다양한 새싹 채소의 생육 및 생리활성 화합물의 함량)

  • Lee, Jin-Hui;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.30 no.3
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    • pp.218-229
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    • 2021
  • Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m-2·s-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.

Protease Activity from Fruit Body of Sarcodon aspratus (능이자실체의 Protease 활성)

  • Cho, Nam-Seok;Cho, Hee-Yeon
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.4
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    • pp.58-65
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    • 2004
  • This study was performed to investigate the protease activity from fruit body of Sarcodon aspratus and its features. The specific protease activity was increased with the increasing purification steps, 2.62 times by desalting, 17 times by CMC column chromatography, 113.8 times by DEAE-Sephadex A-50 column chromatography, and 728.3 times by Sephadex G-75 column chromatography. Proteases were identified as two different enzymes having different isoelectric points at pH 4.35 (its recovery rate 8%) and pH 4.7 (its recovery rate 3.5%). Those proteases were purified by 3,025 folds and 3,257 folds in terms of specific activity. Two proteases having different isoelectric points had similar enzymatic properties. This protease was estimated to be 43,000 daltons of molecular weights by SDS-PAGE. This protease with optimum pH 4 was almost stable in the pH range of 4~7. Optimal temperature of protease activity was 40 to 50℃, and the protease activity was completely inhibited at 70℃ for 30 min.

Optimization of Submerged Culture Conditions for Protease Production and Its Enzymatic Properties (Protease 생산을 위한 최적 배양조건 및 생산된 Protease의 특성)

  • Cho, Hee-Yeon;Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.5
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    • pp.12-19
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    • 2004
  • This study was performed to investigate the optimum condition of protease production from submerged culture of oak mushroom (Lentinula edodes, Sanlim No. 5) and its enzymatic features. Among several combinations of media, the combination of wheat bran, corn flour, water and corn oil (WB+CF+W+ CO) yielded 84.8 U/g of maximum protease activity. This combination of ingredients, in spite of not being particularly protein-rich in comparison to the other media, allowed for good growth of the fungus and maximal protease production. Comparison of different growth medium liquids indicated that demineralized water afforded the best growth of the fungus and the highest protease activity. Acetate buffer and acidified water negatively affected The protease production peaked around 72 hr of incubation, and decreased thereafter. The molecular weights of produced protease were about 45,000 by Sephadex G-75 chromatography. The pH optimum for protease activity was 4, while maximal activity incubated at 37℃ for 1 hr was observed between pH 4~6. The optimum temperature of this protease was 55℃, and the enzyme was active over a broad temperature range (30~60℃), indicating that this protease would be suitable for a wide range of applications where. different pH and temperature are necessary, such as digestive aids, food industry, beer and tannery industries.

Exploring the Possibility of Introducing Modern Physics into Elementary School Science Curriculum (초등학교 과학 교육과정에 현대 물리 도입 가능성 탐색)

  • Park, Jongwon;Yoon, Hye-Gyoung;Lee, Insun
    • Journal of Korean Elementary Science Education
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    • v.41 no.2
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    • pp.199-216
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    • 2022
  • This study explored the possibility of introducing modern physics into the elementary school science curriculum. The study discussed the need of introducing modern physics to elementary school students and examined the results of certain projects and studies on teaching modern physics to elementary school students. Furthermore, this study proposes several teaching and learning techniques to introduce modern physics into the elementary school science curriculum. Modern physics can be linked to various everyday situations experienced by students and can increase their interest and curiosity in science. Additionally, introducing modern physics to elementary school students who are yet to establish a background on the classical view of nature can help them build a new perspective. Recently, several global projects to introduce modern physics at the elementary level have also reported positive results regarding the increase in student understanding and interest in modern physics. The study briefly proposed specific topics and teaching and learning techniques that could be suitable for the elementary school level. These proposals are expected to advance discussions on the possibility of introducing modern physics. However, appropriate follow-up studies are warranted to confirm the possibility and effectiveness of this initiative.

Current Status of the Implementation of Convergence Education in Primary and Secondary Schools (초·중등학교의 융합교육 운영 현황 실태조사)

  • Kwon, Hyuksoo;Kim, Eojin;Park, Hyun Ju;Bae, Youngkwon;Lee, Dongkuk;Lee, Hyungdong;Lee, Hyonyoung;Choi, Sung-Youn;Ham, Hyung-In
    • Journal of Science Education
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    • v.45 no.3
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    • pp.336-348
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    • 2021
  • The goal of this study is to investigate the current status of implementation of convergence education in elementary, middle, and high schools. A survey was conducted on 871 in-service teachers nationwide, and frequency analysis was conducted by school level. Key findings are as follows: first, 449 (51.5%) are found to practice convergence education. Second, the reason for implementing convergence education is the voluntary effort of teachers and the educational necessity for the future society. Third, it was found that convergence education is being implemented centered on science, arts, and social studies as a link between subjects in regular curriculum hours. Fourth, 270 (64%) of teachers who implemented convergence education in response to COVID-19 performed online convergence education, and experienced difficulties in creating class materials and communicating with students. Fifth, the excessive work of teachers, insufficient support for teacher training and research group activities, and lack of various convergence education programs are suggested as reasons for not implementing convergence education. This study hopes to provide implications for policy and implementation for revitalizing convergence education.

Analysis of the Difficulties in the Development of Programs and Class Management in the Hands-on Science Class and Demands for Training of Instructors (생활과학교실 강사의 프로그램 개발과 수업 운영에서 겪는 어려움 및 연수에 대한 요구 분석)

  • Sung-Man, Lim;Han-Sol, Kim;Ha-Na, Choi;Na-Eun, Lee;Seong-Un, Kim
    • Journal of the Korean Society of Earth Science Education
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    • v.15 no.3
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    • pp.322-334
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    • 2022
  • This study attempted to understand the difficulties experienced in program development and class operation of instructors in the hands-on science class and the needs for training based on them. For this study, an online survey was conducted on 193 instructors in the hands-on science class in 2022, and interviews were conducted on 13 instructors. As a result, the difficulties of developing programs for hands-on science class instructors were due to lack of class content, lack of program development budget, lack of equipment necessary for class operation, and difficulty in applying various educational methods such as discussion and practice. The preferred training contents were in the order of the latest science and technology, reconstruction methods of existing programs, and regional specialization technology. In addition, it was found that the difficulties experienced by instructors in class management stemmed from the method of operating hands-on science classes using experience kits. Accordingly, instructor education should be provided in the direction of helping instructors to provide the best education in the situation of the hands-on science classroom.

Hail Risk Map based on Multidisciplinary Data Fusion (다학제적 데이터 융합에 기초한 우박위험지도)

  • Suhyun, Kim;Seung-Jae, Lee;Kyo-Moon, Shim
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.234-243
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    • 2022
  • In Korea, hail damage occurs every year, and in the case of agriculture, it causes severe field crop and cultivation facility losses. Therefore, it is necessary to develop a hail information service system customized for Korea's primary production and crop-growing areas to minimize hail damage. However, the observation of hail is relatively more difficult than that of other meteorological variables, and the available data are also spatially and temporally variable. A hail information service system was developed to understand the temporal and spatial distribution of hail occurrence. As part of this, a hail observation database was established that integrated the observation data from Korea Meteorological Administration with the information from newspaper reports. Furthermore, a hail risk map was produced based on this database. The risk map presented the nationwide distribution and characteristics of hail showers from 1970 to 2018, and the northeastern region of South Korea was found to be relatively dangerous. Overall, hail occurred nationwide, especially in the northeast and some inland areas (Gangwon, Gyeongbuk, and Chungbuk province) and in winter, mainly on the north coast and some inland areas as graupel (small and soft hail). Analyzing the time of day, frequency, and hailstone size of hail shower occurrences by region revealed that the incidence of large hail stones (e.g., 10 cm at Damyang-gun) has increased in recent years and that showers occurred mainly in the afternoon when the updraft was well formed. By integrating multidisciplinary data, the temporal and spatial gap in hail data could be supplemented. The hail risk map produced in this study will be helpful for the selection of suitable crops and growth management strategies under the changing climate conditions.

A Study on Inflammation and Itching of Cyperus rotundus Ethyl Acetate Fractions (향부자 에틸아세테이트 분획물의 염증 및 가려움증에 관한 연구)

  • Do Gyu, Kim;Ji Yeon, Lee;Sohyun, Mun;Yukyung, Kim;Nari, Kim;Ah Reum, Jung;Jun-Hwan, Jang;Jae-Soeb, Lee;Sang Bae, Han;Jun-Tae, Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.48 no.4
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    • pp.303-312
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    • 2022
  • In this study, Cyperus rotundus (C. rotundus) was fractionated into ethyl acetate to identify α-cyperone, a representative indicator, and its ethyl acetate fraction were evaluated to confirm the possibilityas a functional cosmetic ingredient. As a result of HPLC analysis, it was confirmed that the content of α-cyperone was 5.243%. In order to verify the inflammatory relief effect of the ethyl acetate fraction from C. rotundus (EAFC) and α-cyperone, it was confirmed that nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS). Real-time qPCR analysis confirmed inhibition of mRNA expression level of inflammatory factors, IL-1β, IL-6, TNF-α, COX-2 and iNOS. As a results of conducting a clinical study using a simple cosmetic formulation containing EAFC, it was confirmed that the skin irritation stimulated by sodium lauryl sulfate (SLS) was calming and relieving itching. Through these results, it is believed that the C. rotundus can be used as a natural cosmetic ingredient that has the effect of inhibiting inflammation and relieving itching.

A Study of AI Education Program Based on Big Data: Case Study of the General Education High School (빅데이터 기반 인공지능 교육프로그램 연구: 일반계 고등학교 사례를 중심으로)

  • Ye-Hee, Jeong;Hyoungbum, Kim;Ki Rak, Park;Sang-Mi, Yoo
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.23 no.1
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    • pp.83-92
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    • 2023
  • The purpose of this research is to develop a creative education program that utilizes AI education program based on big data for general education high schools, and to investigate its effectiveness. In order to achieve the purpose of the research, we developed a creative education program using artificial intelligence based on big data for first-year general high school students, and carried out on-site classes at schools and a validation process by experts. In order to measure the creative problem-solving ability and class satisfaction of high school students, a creative problem-solving ability test was conducted before and after the program application, and a class satisfaction test was conducted after the program. The results of this study are as follows. First, AI education program based on big data were statistically effective to improve the creative problem solving ability according to independent sample t test about 'problem discovery and analysis', 'idea generation', 'execution plan', 'conviction and communication', and 'innovation tendency' except 'execution', 'the difference between pre- and post-scores of male student and female student' on first year high school students. Secondly, in satisfaction conducted after classes of AI education program based on big data, the average of 'Satisfaction', 'Interest', 'Participation', 'Persistence' were 3.56 to 3.92, and the overall average was 3.78. Therefore, it was investigated that there was a lesson effect of the AI education program based on big data developed in this research.

Quantitative Comparison of Motion Artifacts in PET Images using Data-Based Gating (데이터 기반 게이팅을 이용한 PET 영상의 움직임 인공물의 정량적 비교)

  • Jin Young, Kim;Gye Hwan, Jin
    • Journal of the Korean Society of Radiology
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    • v.17 no.1
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    • pp.91-98
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    • 2023
  • PET is used effectively for biochemical or pathological phenomena, disease diagnosis, prognosis determination after treatment, and treatment planning because it can quantify physiological indicators in the human body by imaging the distribution of various biochemical substances. However, since respiratory motion artifacts may occur due to the movement of the diaphragm due to breathing, we would like to evaluate the practical effect by using the a device-less data-driven gated (DDG) technique called MotionFree with the phase-based gating correction method called Q.static scan mode. In this study, images of changes in moving distance (0 cm, 1 cm, 2 cm, 3 cm) are acquired using a breathing-simulated moving phantom. The diameters of the six spheres in the phantom are 10 mm, 13 mm, 17 mm, 22 mm, 28 mm, and 37 mm, respectively. According to maximum standardized uptake value (SUVmax) measurements, when DDG was applied based on the moving distance, the average SUVmax of the correction effect by the moving distance was improved by 1.92, 2.48, 3.23 and 3.00, respectively. When DDG was applied based on the diameter of the phantom spheres, the average SUVmax of the correction effect by the moving distance was improved by 2.37, 2.02, 1.44, 1.20, 0.42 and 0.52 respectively.