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http://dx.doi.org/10.15230/SCSK.2022.48.4.303

A Study on Inflammation and Itching of Cyperus rotundus Ethyl Acetate Fractions  

Do Gyu, Kim (R&D Center, J2KBIO)
Ji Yeon, Lee (R&D Center, J2KBIO)
Sohyun, Mun (R&D Center, J2KBIO)
Yukyung, Kim (R&D Center, J2KBIO)
Nari, Kim (R&D Center, J2KBIO)
Ah Reum, Jung (R&D Center, J2KBIO)
Jun-Hwan, Jang (R&D Center, J2KBIO)
Jae-Soeb, Lee (R&D Center, J2KBIO)
Sang Bae, Han (College of Pharmacy and Medical Research Center, Chungbuk National University)
Jun-Tae, Bae (R&D Center, J2KBIO)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.48, no.4, 2022 , pp. 303-312 More about this Journal
Abstract
In this study, Cyperus rotundus (C. rotundus) was fractionated into ethyl acetate to identify α-cyperone, a representative indicator, and its ethyl acetate fraction were evaluated to confirm the possibilityas a functional cosmetic ingredient. As a result of HPLC analysis, it was confirmed that the content of α-cyperone was 5.243%. In order to verify the inflammatory relief effect of the ethyl acetate fraction from C. rotundus (EAFC) and α-cyperone, it was confirmed that nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS). Real-time qPCR analysis confirmed inhibition of mRNA expression level of inflammatory factors, IL-1β, IL-6, TNF-α, COX-2 and iNOS. As a results of conducting a clinical study using a simple cosmetic formulation containing EAFC, it was confirmed that the skin irritation stimulated by sodium lauryl sulfate (SLS) was calming and relieving itching. Through these results, it is believed that the C. rotundus can be used as a natural cosmetic ingredient that has the effect of inhibiting inflammation and relieving itching.
Keywords
Cyperus rotundus; alpha-cyperone; anti-inflammatory; nitric oxide; RAW 264.7;
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Times Cited By KSCI : 2  (Citation Analysis)
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