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Effects of dietary protease on immune responses of weaned pigs

  • Lee, Jeong Jae;Kang, Joowon;Park, Sangwoo;Cho, Jin Ho;Oh, Sejong;Park, Dong-Jun;Perez-Maldonado, Rider;Cho, Jee-Yeon;Park, Il-Hun;Kim, Hyeun Bum;Song, Minho
    • Journal of Animal Science and Technology
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    • v.62 no.2
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    • pp.174-179
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    • 2020
  • This experiment was conducted to investigate effects of dietary protease on immune responses of weaned pigs. Weaned pigs (n = 75; 7.06 ± 0.18 kg BW; 28 d old) were randomly assigned to 3 treatments (5 pigs/pen; 5 pens/treatment). Dietary treatments were positive control, a diet with required protein level (PC), negative control, a diet with lower protein level than PC (NC), and NC + 0.02% dietary protease (PRO). The dietary protease used in this experiment was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. The dietary treatments did not contain any ingredients or additives that may provide antibacterial or physiological effects. Pigs were fed respective dietary treatments for 6 weeks. Blood was collected from randomly selected 2 pigs in each pen on d 1, 3, 7, and 14 after weaning. Measurements were number of white blood cells (WBC), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), and C-reactive protein (CRP). Pigs fed PRO had lower WBC on d 7 (14.84 vs 20.42 × 103/μL; p < 0.05) and TNF-α on d 7 (618 vs 889 pg/mL; p = 0.085) and 14 (437 vs 576 pg/mL; p = 0.069) than those fed NC, but there were no differences on WBC and TNF-α between PC and PRO. Pigs fed PRO had lower TGF-β1 on d 3 (630 vs. 1,588 and 1,396 pg/mL; p < 0.05) than those fed PC and NC. However, no differences were found on CRP among dietary treatments. In conclusion, addition of dietary protease reduced inflammatory immune responses of weaned pigs.

Chinese Geography Curriculum Changes and the Organization of Geography Curriculum Standards (중국의 지리교육과정 변천과 지리과정표준의 구성체계)

  • Kang, Chang-Sook
    • Journal of the Korean association of regional geographers
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    • v.18 no.2
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    • pp.217-231
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    • 2012
  • Geography is a core subject for mutual understanding a relationship between Korea, China, and Japan. But the research and report on the Chinese geography education are poor progressed in Korea. This study aims to review the Chinese geography curriculum changes and the organization of Geography Curriculum Standards(GCS). The results of study are as follows. First, from the perspective of the Chinese geography curriculum development, political ideology and changes of society could lead to serious influences on the geography education. It is change through the process stage of prosperity${\rightarrow}$withdrawal${\rightarrow}$promotion${\rightarrow}$development. Second, most recently, Chinese GCS carried out a drastic reform of their geography curriculum emphasized the importance of a student' needs and activity, the organic correlation between the geographical knowledge and skills, and synthesis, practical value of the geography. Third, the organization of GCS consist of an introduction, curriculum objective, contents standard, performance rules. Fourth, the middle school GCS emphasized the importance of a basis of the geographical knowledge and skills in the 4 subjects(the earth and the maps, world geography, Chinese geography, regional geography). Finally, The high school GCS emphasized the importance of a student' occupational course and consideration of regional circumstances.

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The Contents Organization and Description Style of World Geography Textbook "小學萬國地誌" in the Period of Modern Enlightenment (근대계몽기 세계지리 교과서 "소학만국지지(小學萬國地誌)"의 내용체계와 서술방식)

  • Kang, Chang-Sook
    • Journal of the Korean association of regional geographers
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    • v.19 no.4
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    • pp.747-763
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    • 2013
  • Books on world geography, so called "萬國地誌" had an important influence on changing Koreans' views of the world and the nation through the introduction of new world geographical knowledge during the period of modern enlightenment(1894~1910). "小學萬國地誌" was published in 1895 by Hakbu as an early world geography textbook. This study sought to discuss the significance and influence of "小學萬國地誌" by focusing on its contents organization and description style. The findings are as follows. The six regional contents organization adopted the modern regional geographical method by regional scale classification, describing the human geography based on the physical geography, comparing regional characteristics or studying the relationship between human and the nature. In addition, this textbook was written using the kukhanmun style that displays several characteristics of modern printing culture. Many geographical names were written in Korean characters and geographical knowledge was accepted actively from the modern world, subsequently contributing to the generalization of geographical knowledge. This textbook led to the transition of the Koreans' worldview from a China-centric focus by providing practical and specific knowledge about the world, toward the Korean modernization based on the multiple ideologies of social Darwinism and the advent of enlightenment, imperialism and nationalism.

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Analysis on the Content Validity of the Korean Geography Subject College Scholastic Ability Test on the National Curriculum Achievement Standards (한국지리 대학수학능력시험의 교육과정 성취기준에 대한 내용타당도 분석)

  • Kim, Sihwa;Kang, Chang-Sook
    • Journal of the Korean association of regional geographers
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    • v.23 no.1
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    • pp.195-212
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    • 2017
  • This study analyzed on the content validity of the Korean geography subject evaluation questions on the College Scholastic Ability Test(CSAT) from 2014 to 2016 regarding the national curriculum achievement standards. The main results are as follows. First, from 'the knowledge dimension' aspect, both the achievement standard and the CSAT questions showed the highest ratio of 'factual knowledge'. Second, from 'the cognitive process dimension' aspect, the percentage of 'understand' was the highest in the achievement standard, whereas in the CSAT questions, the ratio of 'analyze' and complex type of 'analyze' and 'understand' was the highest. Third, in the result of the analysis of the content validity of the Korean geography CSAT questions through proportion test, all of the analysis targets showed 'a little low'. Finally, the content validity analysis showed that there was a significant difference between the main types of the achievement standards and the CSAT questions in the 'Geomorphological Environment and Ecosystem part' and 'Space of Production and Consumption part' from the cognitive process aspect, which showed relatively low content validity compared to other areas. The results of this study suggest that the achievement standards should be reflected on the Korean geography CSAT questions and it should not be focused on evaluating the learner's analysing ability.

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Applicability Evaluation of Nile Blue A Dye to Korean Termite (Reticulitermes speratus kyushuensis) (국내 서식 흰개미의 개체 및 군체 식별을 위한 Nile Blue A 염색법 적용성 평가)

  • Kim, Si Hyun;Im, Ik Gyun;Chung, Yong Jae
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.333-338
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    • 2020
  • Dye markers are used in the study of subterranean termites to determine colony territories and population sizes. Dyes not only allow the study of termite biology, but can also be used for control purposes. Although Reticulitermes speratus kyushuensis causes damage to wooden historical properties in Korea, the dye markers have not been applied. Therefore, we evaluated the applicability of Nile Blue A dye for R. s. kyushuensis. Filter papers dyed with concentrations of 0.05%, 0.10%, and 0.25% (w/w) were each fed to termite populations through a no-choice feeding test for 1, 3, or 7 days. After 4 weeks, the survival rate, dyeing rate, and maintenance of dyeing were recorded. The results showed high survival rates with lower dye concentrations and shorter feeding periods. Among the combinations of concentrations and days, high survival rates, dyeing rates, and dyeing maintenance periods were found at concentrations of 0.05% and 0.10% for 3 days of feeding. These maximized the survivorship and visibility. The results from the current study can be applied to investigate the ecology and control of R. s. kyushuensis in both the laboratory and field.

Materials Analysis of Furnace Wall Excavated from Songdu-ri Site in Jincheon, Chungbuk (충북 진천 송두리 유적 출토 노벽의 재료과학적 분석)

  • Jang, Won Jin;Han, Min Su
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.421-429
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    • 2020
  • This study examined the manufacturing process of a furnace wall excavated from the Songdu-ri Site in Jincheon, and the difference in material composition between the 11 layers of the wall using physicochemical analyses. Based on microstructure observations, these layers could be largely divided into three groups: an undercalcined first layer, calcined second to ninth layer with evidences of partial heat, and non-fired soil layers from the tenth to the eleventh layer. Particle size analyses revealed that the fired layer constituted a relatively higher content of coarse sand than the non-fired layer. This difference was further confirmed by the results of the curvature coefficient analysis. An analysis of the constituent minerals showed similar overall XRD diffraction patterns between the different layers, but variations in the intensity of the low-temperature and high-temperature minerals. This indicates that the degree of heat was different. The thermal analysis results demonstrated that the heating peak of mullite was only reached in the first and second layers of the wall, thus implying these as the layers to be finally used. Consequently, no significant difference could be observed between the materials of the various layers of the wall. Thus, it can be suggested that the furnace wall was constructed using clay, which had a composition similar to that of the soil present in the area. However, the shape and characteristics of the constituent particles between the layers displayed partial variations, and it is possible that some external materials might have been added.

A Study on Dietary Habits, Body Satisfaction and Nutritional Knowledge by Body Image of Middle School Girl Students in Chungbuk Area (충북 일부지역 여자 중학생의 체형 인식도에 따른 식습관, 체형 만족도 및 영양지식에 관한 연구)

  • Yeon, Jee-Young;Shin, Ki-Yong;Lee, Soon-Kyu;Lee, Hye-Young;Kang, Baeg-Won;Park, Hye-Kyung
    • Korean Journal of Community Nutrition
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    • v.18 no.5
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    • pp.442-456
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    • 2013
  • This study was performed to investigate the dietary habits, body satisfaction and nutritional knowledge according to body image of middle school girl students (n = 284). The subjects were classified as lean, normal and fat groups according to body image. The weight, body mass index (BMI) and the score of body image were significantly higher in the students who recognized their body image as 'fat'. The satisfaction of present body image was significantly lower in students who recognized their body image as 'lean'. The interest in weight control and experience of weight control were significantly higher in students who recognized their body image as 'fat'. The necessity of nutrition education was higher in all groups (lean 67.2%, normal 59.0% and fat 52.8%). The products used to feel sweetened were sweetened ice (68.7%), processed milk (68.3%), confectionery (62.3%), carbonated beverage (55.3%), fermented milk (38.0%) and none (6.0%). The frequency of consumption of fruit juices and fried food was significantly higher in 'normal' compared to 'fat'. There was no significant difference in the frequency of consumption of snack and nutritional knowledge among the three groups. In the multiple regression analysis, the score of body image was negatively associated with the frequency of snack consumption in the 'lean'. The score of body image was positively associated with identification of nutrition labels and an interest in weight control but was negatively associated with satisfaction of present body image in the 'fat'. Based on these results, we conclude that, the middle school girl students need correct recognition of body image and continuous and practical nutrition education in order to maintain healthy dietary habits.

Environmental Contamination and Best Management of Stone-dust from Quarry Mine (석산개발에 따른 주변 환경오염 및 석분토 처리를 위한 연구)

  • Lee, Pyeong-Koo;Youm, Seung-Jun;Kang, Min-Ju
    • Economic and Environmental Geology
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    • v.43 no.4
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    • pp.315-332
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    • 2010
  • All of the water and stone-dust samples with or without flocculant, in and around quarry mines, were analyzed for total concentrations of heavy metals, cyanide(CN), toxic organic compounds and organic phosphorus. Extraction experiments on stone-dust by EDTA and various pH solutions were also carried out, in order to evaluate the contaminant leaching from the long-term heaped stone-dust within quarry mines. The concentrations of $Cr^{6+}$, Hg, CN, TCE/PCE and total phosphorus in all samples (water and stone-dust) were under detection limits, confirming no environmental contamination from stone-dust in quarry mine areas. Lead and cadmium were not detected in all water samples. Copper and zinc were found in some water samples, and arsenic was detected in a few water samples. But they also showed levels much lower than the drinking water standard. Results of the extraction experiments by EDTA and pH solutions showed that Pb, Cr, Cd, Cu and Zn were leached out in less amounts or under detection limits. Arsenic was detected only at pH 3. From above results, we suggested that environmental contamination by quarry mine development is likely to be minor or negligible.

Comparison analysis of before and after scaling training on grip and pinch strength (스케일링 실습수업 전후 수부의 악력 및 파지력 비교분석)

  • Yoo, Ja-Hea;Lee, Min-Young;Lee, Sang Young
    • Journal of Korean society of Dental Hygiene
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    • v.13 no.1
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    • pp.114-121
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    • 2013
  • Objectives : The purpose of the research is to measure Dental Hygiene students' level of musculoskeletal pain before and after receiving scaling training which involves repetitive operations and examine effects the operations have on the students' grip and pinch strength. Methods : 15 subjects in Dental Hygiene at Y university located in Chungbuk were tested on November 2, 2011. Results : 12 students had a musculoskeletal pain before receiving scaling training. However, all the 15 students had a musculoskeletal pain after the training. Body part the largest number of the students complained of the pain on was shoulders(37.9%) followed by neck(20.8%), waist(13.9%), wrists(10.3%), then back(6.9%) before receiving the training. Then the result showed that shoulders are where the largest number of students(24.1%) felt the pain, followed by neck(17.2%), wrists and fingers(13.8%), waist(12.1%), back and lower arms(6.9%), then palms(5.2%) after the training. Visual Analogue Scale(VAS) increased to $6.35{\pm}2.09$ after receiving the training from $3.31{\pm}3.38$ and this shows statistically significant difference(p<.001). Upon inspection, average grip strength decreased to $23.23{\pm}5.79kg$ from $25.00{\pm}4.51kg$ after receiving the training and this shows statistically significant difference(p<.05). Key pinch was the strongest among the three pinch strengths followed by tripod pinch then tip pinch. Average of all the three pinch strengths showed to have statistically significant difference before and after the training(p<.05); Tip pinch from $6.53{\pm}1.36$ to $5.87{\pm}1.3$, key pinch from $13.47{\pm}2.5$ to $12.53{\pm}2.30$, and tripod pinch from $9.27{\pm}3.04$ to $8.03{\pm}2.72$. Conclusions : The result showed that scaling training has effects on Dental Hygiene students' musculoskeletal subjective symptoms and their grip and pinch strength changes after receiving the training.

Solubilization Characteristics of Piggery Slurry by Different Storage type and Temperature Conditions (돈분 슬러리의 저장형태 및 온도에 따른 가용화 특성)

  • Park, Woo-Kyun;Jun, Hang-Bae;Park, Noh-Back;Hong, Seung-Gil
    • Korean Journal of Environmental Agriculture
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    • v.29 no.4
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    • pp.348-353
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    • 2010
  • The current study investigated the fate of organic matter in piggery slurry under two different store systems(closed store system and open store system) in association with different temperature. Thirty days after storing in both systems at $20^{\circ}C$, it was observed that the content of organic matter remained in piggery slurry with closed store system was twice more than that with open store system implying more efficient degradation of organic matter with open store system. Temperature also influenced on the organic matter degradation in piggery slurry as shown decline in TS and VS contents as the temperature increased. With store at $35^{\circ}C$, 29% of initial organic matter was reduced while there was only 23% reduction of organic matter at $20^{\circ}C$. There was no difference in the type of organic fatty acids(VFAs) produced under the range of temperature(20, $35^{\circ}C$) simulating summer condition. Increases in organic fatty acids contents with hydrolysis and acid producing microbial was observed from 15 days after initiating store of the piggery slurry and the total organic acid amount produced 30 days after store was $2,829\;mg{\cdot}COD/L$ and $9,123\;mg{\cdot}COD/L$ at $20^{\circ}C$ and $35^{\circ}C$, respectively. These corresponded to 5.4% and 17.4% of the initial organic matter contents in piggery slurry, respectively.