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The Race Differentiation of Phytophthora capsici in Korea (국내 고추 역병균의 병원성 분화)

  • Lee, Sang-Jun;Park, Yong-Ju;Kim, Heung-Tae;Kim, Byung-Sup
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.153-157
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    • 2010
  • This study was examined to identify the race differentiation and distribution of mating type on Phytophthora capsici population in Korea. One hundred forty three isolates of P. capsici were collected from several locations of Korea in 2005-2007. In 2005, 20 isolates of P. capsici were collected and surveyed as A1 mating type of 75% and A2 mating type of 25%. In 2006, a total of 91 isolates were collected and separated as A1 mating type of 49.0%, A2 mating type of 42.9% and S type (sterile) of 3.3%. Isolates obtained in 2007 were similar to 2006 results. Totally, ratio of mating type of 153 isolates was confirmed that A1 type was 56.6%, A2 type was 39.2%, and S type was 4.2%. Thirteen pepper cultivars with different pathogenic response to 3 typical isolates having different mating were screened among 50 pepper cultivars and determined as race differential cultivars for investigation. The 11 races of P. capsici were found by using 13-race differential cultivars. These results indicated that at least 11 races of P. capsici are existed and confirmed race differentiation of P. capsici in pepper.

Bacterial Spot Disease of Green Pumpkin by Pseudomonas syringae pv. syringae (Pseudomonas syringae pv. syringae에 의한 애호박 세균점무늬병)

  • Park, Kyoung-Soo;Kim, Young-Tak;Kim, Hye-Seong;Lee, Ji-Hye;Lee, Hyok-In;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.22 no.3
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    • pp.158-167
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    • 2016
  • A pathogen that causes a new disease on green pumpkin in the nursery and the field was characterized and identified. Symptoms of the disease on green pumpkin were water soaking lesions and spots with strong yellow halo on leaf, brown lesion on flower, and yellow spot on fruit. The bacterial isolates from the leaf spot were pathogenic on the 8 curcubitaceae crop plants, green pumpkin, figleaf gourd, wax gourd, young pumpkin, zucchini, cucumber, melon, and oriental melon, whereas they did not cause the disease on sweet pumpkin and watermelon. They were Gram-negative, rod shape with polar flagella, fluorescent on King's B agar and LOPAT group 1a by LOPAT test. Their Biolog substrate utilization patterns were similar to Pseudomonas syringae pv. syringae's in Biolog database. Phylogenetic trees with 16S rRNA gene sequences and multilocus sequence typing (MLST) with nucleotide sequences of 4 housekeeping genes, gapA, gltA, gyrB, rpoD and those of P. syringae complex strains in the Plant Associated and Environmental Microbes Database (PAMDB) showed that the green pumpkin isolates formed in the same clade with P. syringae pv. syringae strains. The clade in MLST tree was in the genomospecies 1 group. The phenotypic and genotypic characteristics suggested that the isolates from green pumpkin lesion were P. syringae pv. syringae.

Current Status and Characteristics of Highly Pathogenic Avian Influenza (고병원성 가금인플루엔자의 최근 발생동향과 질병 특성)

  • Kim, J.H.;Sung, H.W.;Kwon, Y.K.;Lee, Y.J.;Choi, J.G.;Cho, S.J.;Kim, M.C.;Lee, E.K.;Jang, H.;Wee, S.H.;Mo, I.P.;Song, C.S.;Park, J.M.
    • Korean Journal of Poultry Science
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    • v.31 no.2
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    • pp.119-128
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    • 2004
  • Highly Pathogenic Avian Influenza (HPAI) is a very acute systemic disease in poultry, particularly in chickens and turkeys caused by HPAI viruses. An outbreak of HPAI caused by subtype H5N1, was first reported in a broiler breeder farm on December 10, 2003 in Korea, although there had been twenty one outbreaks of the disease reported in the world before. Since mid-December 2003, eight Asian countries have confirmed outbreaks of HPAI due to the same subtype. The outbreak has also resulted in at least twenty three fatal human cases in Vietnam and Thailand as of May 17, 2004 according to the WHO. Regarding the first outbreak of recent Asian HPAI, it has been suspected that some Asian countries with the exception of Korea and Japan veiled the fact of HPAI outbreaks since the last half of 2003, even though it was first reported in Korea. There have been total nineteen outbreaks of HPAI among chicken and duck farms in 10 provinces in Korea since Dec. 2003 and approximately 5,280,000 birds were slaughtered from 392 farms for eradication of the disease and preemptive culling. The origin of the H5Nl HPAI virus introduced into the country are unknown and still under epidemiological investigation. Current status of outbreaks and characteristics of HPAI will be reviewed and discussed on the basis of genetic, virological, clinicopathological, and ecological aspect, as well as future measures for surveillance and prevention of the disease in Korea.

A Quality Assurance on Digital Chest Radiography in Medical Institution for Pneumoconiosis : Compared with Analog Radiography (진폐요양기관의 흉부 디지털촬영과 아날로그촬영의 정도관리 비교)

  • Lee, Won-Jeong;Ko, Kyung-Sun;Park, Jai-Soung;Kim, Sung-Jin;Chu, Sang-Deok;Park, So-Young;Choi, Byung-Soon
    • Journal of radiological science and technology
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    • v.33 no.2
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    • pp.85-91
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    • 2010
  • Digital radiography has been replacing rapidly the analog radiography for diagnosis of pneumoconiosis. The purpose of this study is to compare quality control of digital radiography (DR) and analog radiography (AR) for chest radiography in medical institution for pneumoconiosis (MIP) For the first time, we visited MIP to evaluate the chest radiography which is used for patients with pneumoconiosis, including equipment, technical parameters and reading environment. There were 33 institutions. DR and AR were installed in 24 and 9 institutions, respectively. Between DR and AR, we compared the radiological technique (RT), image quality (IQ) and reading environment (RE) to use the guideline published by Occupational Safety and Health Research Institute (OSHRI). The image quality was rated by two experienced chest radiologists for pneumoconiosis with certified from OSHRI. The chest radiography equipment was not significantly difference between AR and DR, but there were significantly difference in tube voltage and grid ratio used for chest radiography except to tube current, exposure time. Statistically, DR is significantly higher in RT(70.3 vs. 43.8, p = 0.009), RE(77.7 vs. 33.3, p = 0.004) than AR, but it's not significantly difference in IQ (65.6 vs. 52.8, p = 0.050). AR and DR in RT were passed 33.3%, 75.0% respectively (p = 0.044) and 44.4%, 79.2% (p = 0.090) in IQ and 44.4%, 91.7% (p = 0.009) in RE. In MIP, DR needs to replace AR in diagnosis of pneumoconiosis.

Change Detection Using Spectral Unmixing and IEA(Iterative Error Analysis) for Hyperspectral Images (IEA(Iterative Error Analysis)와 분광혼합분석기법을 이용한 초분광영상의 변화탐지)

  • Song, Ahram;Choi, Jaewan;Chang, Anjin;Kim, Yongil
    • Korean Journal of Remote Sensing
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    • v.31 no.5
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    • pp.361-370
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    • 2015
  • Various algorithms such as Chronochrome(CC), Principle Component Analysis(PCA), and spectral unmixing have been studied for hyperspectral change detection. Change detection by spectral unmixing offers useful information on the nature of the change compared to the other change detection methods which provide only the locations of changes in the scene. However, hyperspectral change detection by spectral unmixing is still in an early stage. This research proposed a new approach to extract endmembers, which have identical properties in temporally different images, by Iterative Error Analysis (IEA) and Spectral Angle Mapper(SAM). The change map obtained from the difference of abundance efficiently showed the changed pixels. Simulated images generated from Compact Airborne Spectrographic Imager (CASI) and Hyperion were used for change detection, and the experimental results showed that the proposed method performed better than CC, PCA, and spectral unmixing using N-FINDR. The proposed method has the advantage of automatically extracting endmembers without prior information, and it could be applicable for the real images composed of many materials.

Establishment of Critical Ranges of Inorganic Nutrition Contents in Leaves of Watermelon(Cucurbita citrullus L.) in Protected Cultivation (시설재배 수박 엽 적정양분함량 설정)

  • Lee, Ju-Young;Park, Jae-Hong;Jang, Byoung-Choon;Lee, Ki-Sang;Hyun, Byung-Keun;Hwang, Seon-Woong;Yoon, Young-Sang;Song, Beom-Heon
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.3
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    • pp.158-163
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    • 2008
  • This study was carried out to elucidate the relationship between yields and inorganic nutrient contents, and then establish of critical range of inorganic nutrient contents in leaves of watermelon in protected cultivation in Gochang from 2004 to 2006. In considering the yields and nutrient contents of watermelon, the critical ranges of macro-nutrient contents in leaves of watermelon at 20 days after transplanting were in N 5.0~6.6%, P 0.30~0.57%, K 3.5~4.2%, Ca 1.7~3.8% and Mg 0.20~0.42%, respectively. The critical ranges of micronutrient content, such as Fe, Mn, Zn, Cu and B, were not found the regular trend with different growth stages. However, the critical range of micronutrient contents in leaves of watermelon at 20 day after transplanting were in Fe 96~128, Mn 67~201, Zn 40~60, Cu 6~9 및 B $41{\sim}82mg\;kg^{-1}$, respectively. Finally, these results might be used at the indicator for critical nutrient contents for diagnosis of nutritional disorder in watermelon in protected cultivation.

Evaluation of Biomass and Nitrogen Nutrition of Tobacco under Sand Culture by Reflectance Indices of Ground-based Remote Sensors (지상원격측정 센서의 반사율 지표를 활용한 사경재배 연초의 생체량 및 질소영양 평가)

  • Kang, Seong-Soo;Jeong, Hyun-Cheol;Jeon, Sang-Ho;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.2
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    • pp.70-78
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    • 2009
  • Remote sensing technique in agriculture can be used to identify chlorophyll content, biomass, and yield caused from N stress level. This study was conducted to evaluate biomass, N stress levels, and yield of tobacco (Nicotiana tabacum L.) under sand culture in a plastic film house using ground-based remote sensors. Nitrogen rates applied were 40, 60, 80, 100, 120, and 140 percent of N concentration in the Hoagland's nutrient solution. Sensor readings for reflectance indices were taken at 30, 35, 40, 45, 50 and 60 days after transplanting(DAT). Reflectance indices measured at 40th DAT were highly correlated with dry weight(DW) of tobacco leaves and N uptake by leaves. Especially, green normalized difference vegetation index(gNDVI) from spectroradiometer and aNDVI from Crop Circle passive sensor were able to explain 85% and 84% of DW variability and 85% and 92% of N uptake variability, respectively. All the reflectance indices measured at each sampling date during the growing season were significantly correlated with tobacco yield. Especially the gNDVI derived from spectroradiometer readings at the 40th DAT explained 72% of yield variability. N rates of tobacco were distinguished by sufficiency index calculated using the ratio of reflectance indices of stress to optimum plot of N treatment. Consequently results indicate that the reflectance indices by ground-based remote sensor can be used to predict tobacco yield and recommend the optimum application rate of N fertilizer for top dressing of tobacco.

Impacts of Cover Crops on Early Growth, Nitrogen Uptake and Carbohydrate Composition of Pepper Plants (고추의 초기생장, 질소흡수 및 탄수화물 합성에 대한 녹비작물 시용효과)

  • Sung, Jwa-Kyung;Lee, Sang-Min;Lee, Yong-Hwan;Choi, Du-Hoi;Kim, Tae-Wan;Song, Beom-Heon
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.1
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    • pp.44-49
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    • 2008
  • Sufficient inorganic nitrogen supply for crop growth is crucial for economically sustainable organic farming. The effects of an application of cover crop biomass on crop growth, nitrogen utilization and carbohydrate composition were investigated during early stage. Short-term changes in soil nitrogen after incorporating fresh hairy vetch and rye shoots were measured. The inorganic nitrogen from cover crops reached the peak at 15 ($NH_4-N$) and 24 ($NO_3-N$) days after incorporation, and then decreased rapidly. The highest concentration of soil nitrate showed at 27 days of incorporation in hairy vetch and at 18 days in rye, and three fold differences exhibited between two treatments. Crop growth under hairy vetch or rye incorporation significantly differed. At 20 DAT, dry matter production in NPK and hairy vetch was about two fold greater than that in rye. Difference in decomposing rates of hairy vetch and rye had also influence on nitrogen status in leaves and roots of pepper plants. Total nitrogen was greater in NPK and hairy vetch than in rye until 20 DAT, whereas inorganic nitrogen (nitrate and nitrite) concentration was higher in rye. Temporal changes in soluble sugars and starch in pepper plants among treatments were similar, although difference in the amount existed. It was suggested that hairy vetch as an alternative nitrogen source promoted crop growth and mineral utilization during early growth stage, whereas an obvious effect in rye was not found.

Analysis of Contributory Factors in Causing Crashes at Rural Unsignalized intersections Based on Statistical Modeling (지방부 무신호교차로 교통사고의 영향요인 분석 및 통계적 모형 개발)

  • PARK, Jeong Soon;OH, Ju Taek;OH, Sang Jin;KIM, Young Jun
    • Journal of Korean Society of Transportation
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    • v.34 no.2
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    • pp.123-134
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    • 2016
  • Traffic accident at intersections takes 44.3% of total number of accidents on entire road network of Korea in 2014. Although several studies addressed contributory factors of accidents at signalized intersection, very few is known about the factors at rural unsignalized intersections. The objective of this study is therefore to investigate specific characteristics of crashes at rural unsignalized intersection and to identify contributory factors in causing crashes by statistical approach using the Ordered Logistic Regression Model. The results show that main type of car crashes at unsignalized intersection during the daytime is T-bone crashes and the number of crashes at 4-legged intersections are 1.53 times more than that at 3-legged intersections. Most collisions are caused by negligence of drivers and violation of Right of Way. Based upon the analysis, accident severity is modeled as classified by two types such as 3-legged intersection and 4-legged intersection. It shows that contributory factors in causing crashes at rural unsignalized intersections are poor sight distance problem, average daily traffic, time of day(night, or day), angle of intersection, ratio of heavy vehicles, number of traffic violations at intersection, and number of lanes on minor street.

Programmed Reproductive Management Including Presynch-Ovsynch-Resynch Protocol in Dairy Cows (젖소에서 일련의 Presynch-Ovsynch-Resynch Protocol을 이용한 번식관리 예)

  • Kim, Ill-Hwa;Francisco, Nilo
    • Journal of Veterinary Clinics
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    • v.27 no.4
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    • pp.397-401
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    • 2010
  • This study was to evaluate the usefulness of a programmed reproductive management including Presynch-Ovsynch-Resynch protocol in lactating dairy cows. Nine hundred and thirty four cows calved during February to May 2008 were used for this study. Cows received im injections of 25 mg $PGF_{2{\alpha}}$ at $45{\pm}3$ and $59{\pm}3$ days in milk (DIM). Fourteen days later, Ovsynch was initiated with an im injection of $100\;{\mu}g$ GnRH ($73{\pm}3$ DIM), 25 mg $PGF_{2{\alpha}}$ 7 days later ($80{\pm}3$ DIM), $100\;{\mu}g$ GnRH 56 h later and timed artificial insemination (TAI) 16 h after the GnRH injection. Cows showed estrus during the Presynch-Ovsynch, were inseminated artificially according to am-pm rule. Pregnancy was determined on 32 days after AI using ultrasonography. Cows diagnosed pregnant were re-examined for pregnancy by rectal palpation at 60 days after AI. Non-pregnant cows were resynchronized by receiving $100\;{\mu}g$ GnRH, 25 mg $PGF_{2{\alpha}}$ 7 days later, $100\;{\mu}g$ GnRH 56 h later and TAI 16 h after. Cows with estrus since the first AI before the completion of Resynch protocol were also inseminated artificially according to am-pm rule. Pregnancy was determined by the same manner as following the first AI. Fifty five percents of the cows treated showed estrus and received AI before completion of the Presynch-Ovsynch protocol, while 45% received TAI, regardless of estrus exhibition following the completion of the protocol. The pregnancy rate following the first AI was higher in cows that showed estrus and received AI (43.3 and 38.5%) during the Presynch-Ovsynch than cows with TAI (34.6 and 29.6%) on 32 and 60 days after AI (P < 0.01). Sixty six percents of cows diagnosed non-pregnant following the first AI showed estrus before the completion of Resynch protocol and received AI, while 34% received TAI, regardless of estrus exhibition following the completion of the protocol. The pregnancy rate following the second AI was higher in cows that showed estrus before the completion of Resynch protocol and received AI (40.2 and 36.8%) than cows with TAI (21.2 and 18.2%) on 32 and 60 days after AI (P < 0.01). The cumulative pregnancy rates following the first and second AIs were 60.8 and 53.9% on 32 and 60 days after AI, respectively. These data suggest that inclusion of Presynch-Ovsynch-Resynch protocol and estrus detection into the programmed reproductive management might be an alternative option to control dairy cattle breeding.