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http://dx.doi.org/10.5423/RPD.2016.22.3.158

Bacterial Spot Disease of Green Pumpkin by Pseudomonas syringae pv. syringae  

Park, Kyoung-Soo (Nongwoo Bio Co., Ltd.)
Kim, Young-Tak (Nongwoo Bio Co., Ltd.)
Kim, Hye-Seong (Nongwoo Bio Co., Ltd.)
Lee, Ji-Hye (Nongwoo Bio Co., Ltd.)
Lee, Hyok-In (Animal and Plant Quarantine Agency)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Publication Information
Research in Plant Disease / v.22, no.3, 2016 , pp. 158-167 More about this Journal
Abstract
A pathogen that causes a new disease on green pumpkin in the nursery and the field was characterized and identified. Symptoms of the disease on green pumpkin were water soaking lesions and spots with strong yellow halo on leaf, brown lesion on flower, and yellow spot on fruit. The bacterial isolates from the leaf spot were pathogenic on the 8 curcubitaceae crop plants, green pumpkin, figleaf gourd, wax gourd, young pumpkin, zucchini, cucumber, melon, and oriental melon, whereas they did not cause the disease on sweet pumpkin and watermelon. They were Gram-negative, rod shape with polar flagella, fluorescent on King's B agar and LOPAT group 1a by LOPAT test. Their Biolog substrate utilization patterns were similar to Pseudomonas syringae pv. syringae's in Biolog database. Phylogenetic trees with 16S rRNA gene sequences and multilocus sequence typing (MLST) with nucleotide sequences of 4 housekeeping genes, gapA, gltA, gyrB, rpoD and those of P. syringae complex strains in the Plant Associated and Environmental Microbes Database (PAMDB) showed that the green pumpkin isolates formed in the same clade with P. syringae pv. syringae strains. The clade in MLST tree was in the genomospecies 1 group. The phenotypic and genotypic characteristics suggested that the isolates from green pumpkin lesion were P. syringae pv. syringae.
Keywords
Bacterial spot; Green pumpkin; New disease; Pseudomonas syringae pv. syringae;
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