• Biomolecules & Therapeutics
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• v.27 no.2
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• pp.193-200
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• 2019

Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) is a key enzyme for the synthesis of glucosylceramide (GlcCer), which is a main ceramide metabolism pathway in mammalian cells. In this article, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine GCS activity using synthetic non-natural sphingolipid C8-ceramide as a substrate. The reaction products, C8-GlcCer for GCS, could be separated on a C18 column by reverse-phase high-performance liquid chromatography (HPLC). Quantification was conducted using the multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z $588.6{\rightarrow}264.4$ for C8-GlcCer at positive ionization mode. The calibration curve was established over the range of 0.625-160 ng/mL, and the correlation coefficient was larger than 0.999. This method was successfully applied to detect GCS in the human hepatocellular carcinoma cell line (HepG2 cells) and mouse peripheral blood mononuclear cells. We also evaluated the inhibition degree of a known GCS inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) on GCS enzymatic activity and proved that this method could be successfully applied to GCS inhibitor screening of preventive and therapeutic drugs for ceramide metabolism diseases, such as atopic dermatitis and Gaucher disease.

• Journal of Acupuncture Research
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• v.23 no.1
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• pp.71-93
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• 2006

Objectives : This study is performed for proposing reasonable clinical study protocol at International standard clinical study of osteoarthritis of Knee Joint. Methods : We gave the eighteen oriental medicine doctors the questionnaire and the video about female patient who diagnosed Knee joint of osteoarthritis above 60 years old and they tested same patients using differentiation of symptoms and signs. Then they retested it after We educated them with diagnosis education text. Conclusion : The improvement of diagnosis agreement can be possible by developing standardized the same patients using differentiation of symptoms and by reeducation about it.

• Proceedings of the KIPE Conference
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• 2006.06a
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• pp.277-280
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• 2006

Recently, the high frequency isolated boost DC/DC converter has been widely used for the PCS (Power Conditioning System) system because of its small size and low cost. However, the high frequency isolated boost DC/DC converters applied the conventional voltage-fed converter and current-fed converter have the problems such as the high conduction losses and the surge voltage due to the high circulating current and the leakage inductance, respectively. To overcome this problems, in this paper the secondary LLC resonant converter is proposed, and the experimental results of the secondary LLC series resonant converter for boost DC/DC converter are verified on the simulation based on the theoretical analysis and the 1kW experimental prototype.

• Herbal Formula Science
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• v.17 no.2
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• pp.233-238
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• 2009

Objective : To carry out the oriental medicine treatment on a patient with oculomotor nerve palsy accompanied by Diabetes mellitus complication history Methods : We conducted one patient with oculomotor nerve palsy at Won-Kwang University Kwang-Ju Oriental Medical Hospital. We treated patient by herbal medicine, acupuncture, etc. Results : We had an effect that one patient's symptom had improved. Conclusion : We suggest that the oriental medical treatment and management have an effect with oculomotor nerve palsy accompanied by DM complication history.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.10a
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• pp.478-481
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• 2021

With the transition to non-face-to-face classes due to the outbreak of COVID-19, education at school sites is moving in a different direction from traditional lecture-style classes. Accordingly, several online platforms have emerged, and our education is in a transitional period of class change. As a result, after a survey of the currently used online platform, where various contrasts clearly exist, compared with the report before the outbreak of COVID-19, students' perception of ICT usage ability in terms of affective, psychic, and value perception changes want to check

• Journal of Korean Academy of Nursing Administration
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• v.9 no.3
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• pp.367-378
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• 2003

Purpose: The research studied to survey the degree of utilization, satisfactions and problems of the computerization in nursing practice. Method: The subjects were 176 nurses who are working at university hospital in Seoul. The research was operated by 75 questionaries which were developed and modified from precedence study of Kim(1998), No(1996) and Pang(1996). The data was processed by SPSS for window 11.0 and the results analyzed with number, percentile, average, t-test and ANOVA. Results: As the way of using computer system in nursing, the most nurses used that to Dr's order check, treatment activity, laboratory, barcode sticker, medication in order that were connected with order system mutually to other parts. The average degree of contentment from the computerization in nursing practice was presented as 3.855 in the full mark 5. The facility of stock management got the highest points(4.98). The problem was marked 3.12 and the biggest problem is interruption of work from the computer system error(4.03). The contentment of computerization in nursing practice by duty year was reflected a significant difference(F=5,457 P=0.005) and was not related with computerization and computer education(F=0.028 P=0.867, F=2.178 P=0.142).

• Proceedings of the KIEE Conference
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• 2004.07c
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• pp.1909-1911
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• 2004

It is caused that insulation paper, which had got a lot of thermal stress by over-load after installation, should have been deteriorated in electrical and mechanical characteristics. Beside, insulation material is decreased the insulating property and accelerated aging of them in case of dielectric loss when transformers are manufactured with some moisture or transformers would have been them because of moisture-permeation, Therefore, in this study we experienced the influence of moisture content in case of the thermal aged insulation paper. we have measured tan ${\delta}$ and breakdown voltage in the ratio of paper' moisture content before the aging and then taken the same tests again after insulation paper thermally accelerating-aged. There is a purpose to gain data for a life-design and to establish aging mechanism in order to continuously study life expectancy of the insulation paper.

• Natural Product Sciences
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• v.21 no.1
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• pp.66-70
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• 2015

Content analysis of flavonoids (epicatechin, epicatechin gallate, and rutin) present in the leaves of Boehmeria nivea (originating from Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup) and their commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea) was conducted by HPLC. The content of epicatechin, epicatechin gallate, and rutin was highest in the leaves of B. nivea from Geumsan-myeon (0.138 mg/g), Baeksu-eup (1.654 mg/g) and Geumsan-myeon (12.205 mg/g), respectively. With respect to commercial products, the content of epicatechin and epicatechin gallate was highest in ramie tea, with concentrations of 1.879 and 1.090 mg/g, respectively. Given these flavonoid concentrations, B. nivea leaf extracts have the potential to be used as additives in natural medicinal products, health supplements, and beverages.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.1
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• pp.81-87
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• 2013

Quercetin-3-O-${\beta}$-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. In the present study, anti-oxidative and anti-inflammatory effects of QGC were tested in vitro. Epithelial cells obtained from cat esophagus were cultured. When the cells were exposed to acid for 2 h, cell viability was decreased to 36%. Pretreatment with 50 ${\mu}M$ QGC for 2 h prevented the reduction in cell viability. QGC also inhibited the productions of intracellular ROS by inflammatory inducers such as acid, lipopolysaccharide, indomethacin and ethanol. QGC significantly increased the activities of superoxide dismutase (SOD) and catalase, and also induced the expression of SOD2, while it restored the decrease of catalase expression in cells exposed to acid. QGC inhibited NF-${\kappa}B$ translocation, cyclooxygenase-2 expression and $PGE_2$ secretion in cells exposed to acid, which plays an important role in the pathogenesis of esophagitis. The data suggest that QGC may well be one of the promising substances to attenuate oxidative epithelial cell injury and inflammatory signaling in esophagus inflammation.

• Bulletin of the Korean Chemical Society
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• v.34 no.3
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• pp.731-734
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• 2013

Reduced tungsten bronze nanoparticles of ternary and quaternary compounds were prepared by adding sodium and cesium to crystal structures of tungsten trioxides ($Na_xCs_{0.33-x}WO_3$, x = 0, 0.11) while maintaining the overall alkali metal fraction at 0.33, in an attempt to control near infrared (NIR) shielding property in the particular wavelength range of 780 to 1200 nm. The structure and composition analysis of the quaternary compound, $Na_{0.11}Cs_{0.22}WO_3$, revealed that 93.1% of the hexagonal phase was formed, suggesting that both alkali metals were mainly inserted in hexagonal channel. The NIR shielding property for $Na_{0.11}Cs_{0.22}WO_3$ was remarkable, as this material demonstrated efficient transmittance of visible light up to 780 nm and enhancement in NIR shielding because of the blue-shifted absorption maximum in comparison to $Cs_{0.33}WO_3$.