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http://dx.doi.org/10.4196/kjpp.2013.17.1.81

Anti-Oxidative and Anti-Inflammatory Effects of QGC in Cultured Feline Esophageal Epithelial Cells  

Lee, Myeong Jae (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Song, Hyun Ju (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Jeong, Jun Yeong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Park, Sun Young (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Sohn, Uy Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.17, no.1, 2013 , pp. 81-87 More about this Journal
Abstract
Quercetin-3-O-${\beta}$-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. In the present study, anti-oxidative and anti-inflammatory effects of QGC were tested in vitro. Epithelial cells obtained from cat esophagus were cultured. When the cells were exposed to acid for 2 h, cell viability was decreased to 36%. Pretreatment with 50 ${\mu}M$ QGC for 2 h prevented the reduction in cell viability. QGC also inhibited the productions of intracellular ROS by inflammatory inducers such as acid, lipopolysaccharide, indomethacin and ethanol. QGC significantly increased the activities of superoxide dismutase (SOD) and catalase, and also induced the expression of SOD2, while it restored the decrease of catalase expression in cells exposed to acid. QGC inhibited NF-${\kappa}B$ translocation, cyclooxygenase-2 expression and $PGE_2$ secretion in cells exposed to acid, which plays an important role in the pathogenesis of esophagitis. The data suggest that QGC may well be one of the promising substances to attenuate oxidative epithelial cell injury and inflammatory signaling in esophagus inflammation.
Keywords
NF-${\kappa}B$; $PGE_2$; QGC; ROS;
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1 Lee JS, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Kim HJ, Hahm KB. Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett's esophagus: clue for the chemoprevention of esophageal carcinoma by antioxidants. Mutat Res. 2001;480-481:189-200.   DOI   ScienceOn
2 Stickle RL, Epperly MW, Klein E, Bray JA, Greenberger JS. Prevention of irradiation-induced esophagitis by plasmid/liposome delivery of the human manganese superoxide dismutase transgene. Radiat Oncol Investig. 1999;7:204-217.   DOI
3 Li YM, Chan HY, Huang Y, Chen ZY. Green tea catechins upregulate superoxide dismutase and catalase in fruit flies. Mol Nutr Food Res. 2007;51:546-554.   DOI   ScienceOn
4 Doronicheva N, Yasui H, Sakurai H. Chemical structureependent differential effects of flavonoids on the catalase activity as evaluated by a chemiluminescent method. Biol Pharm Bull. 2007;30:213-217.   DOI   ScienceOn
5 Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004;96:229-245.   DOI   ScienceOn
6 Reddy DB, Reddanna P. Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-kappaB and MAPK activation in RAW 264.7 macrophages. Biochem Biophys Res Commun. 2009;381:112-117.   DOI   ScienceOn
7 Chow JM, Shen SC, Huan SK, Lin HY, Chen YC. Quercetin, but not rutin and quercitrin, prevention of $H_{2}O_{2}$-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages. Biochem Pharmacol. 2005;69:1839-1851.   DOI   ScienceOn
8 Kahraman A, Erkasap N, Kaken T, Serteser M, Aktepe F, Erkasap S. The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology. 2003; 183:133-142.   DOI   ScienceOn
9 Milkes D, Gerson LB, Triadafilopoulos G. Complete elimination of reflux symptoms does not guarantee normalization of intraesophageal and intragastric pH in patients with gastroesophageal reflux disease (GERD). Am J Gastroenterol. 2004; 99:991-996.   DOI   ScienceOn
10 Song HJ, Shin CY, Oh TY, Min YS, Park ES, Sohn UD. Eupatilin with heme oxygenase-1-inducing ability protects cultured feline esophageal epithelial cells from cell damage caused by indomethacin. Biol Pharm Bull. 2009;32:589-596.   DOI   ScienceOn
11 Myhre O, Andersen JM, Aarnes H, Fonnum F. Evaluation of the probes 2',7'-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. Biochem Pharmacol. 2003;65:1575-1582.   DOI   ScienceOn
12 Zarling EJ. A review of reflux esophagitis around the world. World J Gastroenterol. 1998;4:280-284.   DOI
13 Cappell MS. Clinical presentation, diagnosis, and management of gastroesophageal reflux disease. Med Clin North Am. 2005; 89:243-291.   DOI   ScienceOn
14 Farhadi A, Fields J, Banan A, Keshavarzian A. Reactive oxygen species: are they involved in the pathogenesis of GERD, Barrett's esophagus, and the latter's progression toward esophageal cancer? Am J Gastroenterol. 2002;97:22-26.   DOI   ScienceOn
15 Graziani G, D'Argenio G, Tuccillo C, Loguercio C, Ritieni A, Morisco F, Del Vecchio Blanco C, Fogliano V, Romano M. Apple polyphenol extracts prevent damage to human gastric epithelial cells in vitro and to rat gastric mucosa in vivo. Gut. 2005;54:193-200.   DOI   ScienceOn
16 Bell RC, Freeman KD. Clinical and pH-metric outcomes of transoral esophagogastric fundoplication for the treatment of gastroesophageal reflux disease. Surg Endosc. 2011;25:1975- 1984.   DOI
17 Jimenez P, Piazuelo E, Sanchez MT, Ortego J, Soteras F, Lanas A. Free radicals and antioxidant systems in reflux esophagitis and Barrett's esophagus. World J Gastroenterol. 2005;11:2697- 2703.   DOI
18 De Souza LF, Barreto F, da Silva EG, Andrades ME, Guimaraes EL, Behr GA, Moreira JC, Bernard EA. Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: involvement of high glucose and PPARgamma. Life Sci. 2007;81:153-159.   DOI   ScienceOn
19 McCarroll JA, Phillips PA, Park S, Doherty E, Pirola RC, Wilson JS, Apte MV. Pancreatic stellate cell activation by ethanol and acetaldehyde: is it mediated by the mitogenctivated protein kinase signaling pathway? Pancreas. 2003;27: 150-160.   DOI   ScienceOn
20 Min YS, Bai KL, Yim SH, Lee YJ, Song HJ, Kim JH, Ham I, Whang WK, Sohn UD. The effect of luteolin-7-O-beta-Dlucuronopyranoside on gastritis and esophagitis in rats. Arch Pharm Res. 2006;29:484-489.   DOI
21 Hernandez-Ledesma B, Hsieh CC, de Lumen BO. Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages. Biochem Biophys Res Commun. 2009;390:803-808.   DOI   ScienceOn
22 Yamaguchi T, Yoshida N, Tomatsuri N, Takayama R, Katada K, Takagi T, Ichikawa H, Naito Y, Okanoue T, Yoshikawa T. Cytokine-induced neutrophil accumulation in the pathogenesis of acute reflux esophagitis in rats. Int J Mol Med. 2005; 16:71-77.
23 Al-Ashy R, Chakroun I, El-Sabban ME, Homaidan FR. The role of NF-kappaB in mediating the anti-inflammatory effects of IL-10 in intestinal epithelial cells. Cytokine. 2006;36:1-8.   DOI   ScienceOn
24 Masaki H. Role of antioxidants in the skin: anti-aging effects. J Dermatol Sci. 2010;58:85-90.   DOI   ScienceOn
25 Itoh T, Hamada N, Terazawa R, Ito M, Ohno K, Ichihara M, Nozawa Y, Ito M. Molecular hydrogen inhibits lipopolysaccharide/ interferon $\gamma$ -induced nitric oxide production through modulation of signal transduction in macrophages. Biochem Biophys Res Commun. 2011;411:143-149.   DOI   ScienceOn
26 Hang CH, Shi JX, Li JS, Li WQ, Yin HX. Up-regulation of intestinal nuclear factor kappa B and intercellular adhesion molecule-1 following traumatic brain injury in rats. World J Gastroenterol. 2005;11:1149-1154.   DOI
27 Odeleye OE, Eskelson CD, Mufti SI, Watson RR. Vitamin E inhibition of lipid peroxidation and ethanol-mediated promotion of esophageal tumorigenesis. Nutr Cancer. 1992;17:223-234.   DOI   ScienceOn
28 Dimayuga FO, Wang C, Clark JM, Dimayuga ER, Dimayuga VM, Bruce-Keller AJ. SOD1 overexpression alters ROS production and reduces neurotoxic inflammatory signaling in microglial cells. J Neuroimmunol. 2007;182:89-99.   DOI   ScienceOn
29 Sugino N. The role of oxygen radical-mediated signaling pathways in endometrial function. Placenta. 2007;28 Suppl A:S133- 136.   DOI   ScienceOn
30 Yamamoto T, Lewis J, Wataha J, Dickinson D, Singh B, Bollag WB, Ueta E, Osaki T, Athar M, Schuster G, Hsu S. Roles of catalase and hydrogen peroxide in green tea polyphenol-induced chemopreventive effects. J Pharmacol Exp Ther. 2004;308: 317-323.
31 Wetscher GJ, Hinder RA, Klingler P, Gadenstätter M, Perdikis G, Hinder PR. Reflux esophagitis in humans is a free radical event. Dis Esophagus. 1997;10:29-32.   DOI
32 Allen RG, Tresini M. Oxidative stress and gene regulation. Free Radic Biol Med. 2000;28:463-499.   DOI   ScienceOn
33 Li Y, Wo JM, Su RR, Ray MB, Martin RC. Loss of manganese superoxide dismutase expression and activity in rat esophagus with external esophageal perfusion. Surgery. 2007;141:359-367.   DOI   ScienceOn
34 Moreira AJ, Fraga C, Alonso M, Collado PS, Zetller C, Marroni C, Marroni N, Gonzalez-Gallego J. Quercetin prevents oxidative stress and NF-kappaB activation in gastric mucosa of portal hypertensive rats. Biochem Pharmacol. 2004;68:1939-1946.   DOI   ScienceOn
35 Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74:418-425.   DOI
36 Aviram M. Review of human studies on oxidative damage and antioxidant protection related to cardiovascular diseases. Free Radic Res. 2000;33 Suppl:S85-97.
37 Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet. 1994;344:721-724.   DOI   ScienceOn
38 De Magalhaes JP, Church GM. Cells discover fire: employing reactive oxygen species in development and consequences for aging. Exp Gerontol. 2006;41:1-10.   DOI   ScienceOn
39 Kim KC, Lee C. Curcumin induces downregulation of E2f4 expression and apoptotic cell death in HCT116 human colon cancer cells; Involvement of reactive oxygen species. Korean J Physiol Pharmacol. 2010;14:391-397.   DOI
40 Menon SG, Goswami PC. A redox cycle within the cell cycle: ring in the old with the new. Oncogene. 2007;26:1101-1109.   DOI   ScienceOn
41 Chen KC, Zhou Y, Zhang W, Lou MF. Control of PDGF-induced reactive oxygen species (ROS) generation and signal transduction in human lens epithelial cells. Mol Vis. 2007;13: 374-387.
42 Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ. Distinctive antioxidant and antiinflammatory effects of flavonols. J Agric Food Chem. 2006;54:9798-9804.   DOI   ScienceOn