• Journal of the East Asian Society of Dietary Life
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• v.27 no.2
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• pp.235-242
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• 2017

In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of ${\alpha}$-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction ($20{\mu}g/mL$) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.

• Journal of Species Research
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• v.12 no.spc2
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• pp.7-14
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• 2023

Several genera belonging to the family Acetobacteraceae were generally considered to be acetic acid bacteria (AAB) which produce acetic acid from alcohols using an oxidation pathway. Some species of the family Acetobacteraceae have been of interest to the industry due to their capability to produce vinegar. In 2018-2020, several bacterial strains were isolated from plants, fruits and vinegar in various regions of the Republic of Korea. Based on the 16S rRNA gene sequences, seven species classified into the family Acetobacteraceae were found to be ones unrecorded in the Republic of Korea, including two Asaia species, one Gluconacetobacter species, three Gluconobacter species and one Komagataeibacter species. As a result, we report Asaia lannensis AF11C3 (=KACC 22050) isolated from plant Chrysanthemum zawadskii, Asaia platycodi AF15C2 (=KACC 22051) isolated from plant Isodon inflexus, Gluconacetobacter liquefaciens C23-3 (=KACC 22064) isolated from fruit of Pyrus pyrifolia, Gluconobacter cerinus BGF2-R2 (=KACC 22053) isolated from fruit of Prunus armeniaca, Gluconobacter kondonii FR39A4 (=KACC 22388) isolated from persimmon fruit, Gluconobacter thailandicus FR36C4 (=KACC 22057) isolated from fruit of Pseudocydonia sinensis and Komagataeibacter melaceti SPV-2 (=KACC 22058) isolated from potato vinegar.

• The Korean Journal of Food And Nutrition
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• v.12 no.6
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• pp.559-563
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• 1999

Essential oil of Artemisia lavandulaefolia the chrysanthemum family plant used in the chinese medicine was extracted and antibacterial and antifungal activity with many kinds of the pathogenic bacterium and fungi was experimented by it. Ataphylococcus epidermidis and Streptococcus aureus gram positive bacterium at the concentration of 200ppm and Streptococcus mutans at the concentration of 1,000ppm showed the growth injibition effect of the cell. These showed statistically significant difference(p<0.05) Zymomonas mobilis Entrecoccus faecalis gram negative bacterium at the concentration of 200ppm and Pseudomonas putida at the concentration of 400ppm showedd the growth inhibition effect of the cell)p<0.05) V. Parahaemolyticus at the concentration of 800ppm and Pseudomonas aeruginosa at the concentration of 1,000ppm showed the growth inhibition effect of the cell(p<0.05) Candida albicans and Cryptococcus neoformans yeast-type fungi showed the gorwth inhibition effect of the cell at the concentration of 200ppm(p<0.05) Altenaria mali Aspergillus nidulans and Fusarium oxysporum filamentous fungi took the growth inhibition effect of the cell at the concentration of 600ppm, 400ppm, and 100ppm. respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.6
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• pp.87-100
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• 2016

This research was performed for the purpose of providing useful and base data and preservation strategy of growth characteristics of Rhododendron micranthum. From the result of research, wild growth area of Rhododendron micranthum was discovered near steep forest road on 695~901m in altitude above sea level of Baekdu-daegan National Arboretum. Vegetation colony structure of Site I was resulted in Pinus densiflora(tree stratum M.I.P.=45.3%) is dominants, Betula schmidtii(arborescent M.I.P.=9.8%) is second dominant, and Site III resulted in Pinus densiflora(tree stratum M.I.P.=30.5%) is dominants, Betula schmidtii(arborescent M.I.P.=10.3%) is second dominant. Site II resulted in Betula schmidtii(arborescent M.I.P.=30.4%) is dominants and Pinus densiflora tree(tree stratum M.I.P.=8.3%) is second dominants. For herbs, it was researched that the rate of one year herb of chrysanthemum family(10), rice family(3), sedge(2) was higher than near forest area. Among Species diversity index, that of Shannon was in range of 0.7348~1.1090. After comparing this with similar area, this value was relatively low. Dominance analysis result was analyzed that various groups are evenly distributed. Monthly highest average temperature is $23.9^{\circ}$ in Site A and Site B, and monthly lowest average temperature is $-4.5^{\circ}C$ in Site I and $-4.7^{\circ}C$ in Site II. Monthly maximum average humidity is 85.6% in Site I and 83.2% in Site II. Monthly minimum average humidity is 60.9% in Site I and 60.8% in Site II and it showed that these 2 area are located far away but have similar temperature and humidity characteristic and climate environment is similar. It was analyzed that humidity is high with the result of annual average humidity is over 60%. In growth characteristic, it showed that doubt damage of Stephanitis pyrioides, exposure of roots, peeled bark was discovered and supplement on this is urgent.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.17-17
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• 2023

Chrysanthemum zawadskii (C. zawadskii) is used in traditional East Asian medicine for the treatment of various diseases, including inflammatory disease. However, it has remained unclear whether extracts of C. zawadskii inhibit inflammasome activation in macrophages. The present study assessed the inhibitory effect of an ethanol extract of C. zawadskii (CZE) on the activation of the inflammasome in macrophages and the underlying mechanism. Bone marrow[-derived macrophages (BMDMs) were obtained from wild-type C57BL/6 mice. The release of IL-1β and lactate dehydrogenase in response to nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome activators, such as ATP, nigericin and monosodium urate (MSU) crystals, was significantly decreased by CZE in lipopolysaccharide(LPS)-primed BMDMs. Western blotting revealed that CZE inhibited ATP-induced caspase-1 cleavage and IL-1β maturation. To investigate whether CZE inhibits the priming step of the NLRP3 inflammasome, we confirmed the role of CZE at the gene level using RT-qPCR. CZE also downregulated the gene expression of NLRP3 and pro-IL-1β as well as NF-κB activation in BMDMs in response to LPS. Apoptosis associated speck-like protein containing a caspase-recruitment domain (CARD) oligomerization and speck formation by NLRP3 inflammasome activators were suppressed by CZE. By contrast, CZE did not affect NLR family CARD domain containing protein 4 (NLRC4) or absent in melanoma 2 (AIM2) inflammasome activation in response to Salmonella typhimurium and poly(dA:dT) in LPS-primed BMDMs, respectively. The results revealed that three key components of CZE, namely linarin, 3,5-dicaffeoylquinic acid and chlorogenic acid, decreased IL-1β secretion in response to ATP, nigericin and MSU. These findings suggest that CZE effectively inhibited activation of the NLRP3 inflammasome.

• Korean Journal of Heritage: History & Science
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• v.46 no.2
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• pp.46-69
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• 2013

The results of study on the meaning landscape and environmental design techniques of the Byulup, Yoohoedang garden(Hageowon) based on the story in the collection of Kwon Yi-jin (Yoohoedangjip, 有懷堂集), are as below. First, Yoohoedang Kwon Yi-jin (有懷堂 權以鎭 : 1668~1734) constructed a Byulup garden consisting of ancestor grave, Byulup, garden, and a school, through 3 steps for 20 years in the back hill area of Moosoo-dong village, south of Mountain Bomun in Daejeon. In other words, he built the Byulup(別業, Yoohoedang) by placing his father's grave in the back hill of the village, and then constructed Yoegeongam(餘慶菴) and Geoupjae(居業齋) for protection of the pond(Napoji, 納汚池), garden(Banhwanwon, 盤桓園), and ancestor graves, and descendants' studying in the middle stage. He built an extension in Yoohoedang and finally completed the large-size garden (Hageowon) by extending the east area. Second, in terms of geomancy sense, Yoohoedang Byulup located in Moosoo-dong village area is the representative example including all space elements such as main living house (the head family house of Andong Kwon family), Byulup (Yoohoedang), ancestor graves, Hagoewon (garden) and Yoegeongam (cemetery management and school) which byulup type Byulseo should be equipped with. Thirdly, there are various meaning landscape elements combining the value system of Confucianism, Buddhism and Taoism value, including; (1) remembering parents, (2) harmonious family, (3) integrity, (4) virtue, (5) noble personality, (6) good luck, (7) hermit life, (8) family prosperity and learning development, (9) grace from ancestors, (10) fairyland, (11) guarding ancestor graves, and (12) living ever-young. Fourth, after he arranged ancestor graveyard in the back of the village, he used surrounding natural landscapes to construct Hagoewon garden with water garden consisting of 4 mountain streams and 3 ponds for 13 years, and finally completed a beautiful fairyland with 5 platforms, 3 bamboo forests, as well as the Seokgasan(石假山, artificial hill). Fifth, he adopted landscape plantation (28 kinds; pine, maple, royal azalea, azalea, persimmon tree, bamboo, willow, pomegranate tree, rose, chinensis, chaenomeles speciosa, Japanese azalea, peach tree, lotus, chrysanthemum, peony, and Paeonia suffruticosa, etc.) to apply romance from poetic affection, symbol and ideal from personification, as well as plantation plan considering seasonal landscapes. Landscape rocks were used by intact use of natural rocks, connecting with water elements, garden ornament method using Seokyeonji and flower steps, and mountain Seokga method showing the essence of landscape meanings. In addition, waterscape are characterized by active use of water considering natural streams and physio-graphic condition (eastern valley), ecological corridor role that rhythmically connects each space of the garden and waterways following routes, landscape meaning introduction connecting 'gaining knowledge by the study of things' values including Hwalsoodam(活水潭, pond), Mongjeong(蒙井, spring), Hosoo(濠水, stream), and Boksoo(?水, stream), and sensuous experience space construction with auditory and visualization using properties of landscape matters.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.5-11
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• 2017

18 flowers of Compositae family were collected and extracted in aqueous methanol (MeOH). The concentrated extract was partitioned into n-hexane, ethyl acetate (EtOAc), n-BuOH, and water fractions. The extract and fractions were evaluated for total phenolics, total flavonoids, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and tyrosinase inhibition activity. n-Hexane and EtOAc fractions of Aster yomena, n-hexane fraction of Cosmos bipinnatus White, n-hexane and EtOAc fractions of C. bipinnatus Pink showed high total phenolics. And EtOAc fractions of A. yomena, C. bipinnatus White, C. bipinnatus Red, C. morifolium Froggy, and C. morifolium Himaya exhibited high total flavonoids. EtOAc fractions of A. yomena, C. bipinnatus White, C. bipinnatus Pink, C. morifolium Yellowmable, and MeOH extract of C. morifolium Rosa significantly scavenged DPPH radical. EtOAc fractions of C. chinensis, C. bipinnatus White, C. bipinnatus Red, C. morifolium Himaya, and C. morifolium Hongsim highly inhibited the tyrosinase activity. A. yomena, C. bipinnatus White, C. bipinnatus Pink, C. bipinnatus Red and C. morifolium Himaya are evaluated as good source for whitening cosmetics materials.

• Korean Journal of Heritage: History & Science
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• v.53 no.1
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• pp.150-167
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• 2020

The purpose of this paper is to share the status and introduce a scientific analysis of the GeumguJagi (gilt-silver overlay porcelain, 金釦瓷器) excavated from Seongneung (石陵). This analysis aimed to highlight the GeumguJagi excavated from the Royal Tombs of Goryeo with a clear lower year (1237) and to aid research into the GeumguJagi. In 2001, the National Institute of Cultural Heritage excavated and investigated Seongneung in Heejong (熙宗). Various artifacts such as celadon, gold, bronze, and iron products were collected from the chambers inside of the tomb. There were a total of 160 celadon items including bowls, dishes, glasses, and saucers. Of those, there were 58 celadon items (including fragments) with metal frames on the openings. These consisted of bowls, plates, lids, and saucers. Until recently, in various exhibitions and papers, only one GeumguJagi was known to have been excavated from Seongneung, which was a . However, the survey identified a number of further GeumguJagis. It had been understood from inherited and excavated products that the materials used for ornaments were restricted to high-quality celadon. However, this study confirmed that the excavation of Seongneung demonstrated the use of various other materials for different models and qualities of GeumguJagis. It can be said that it is characteristic that various models and quality are confirmed together through the excavation of Seongneung. A scientific analysis was carried out that selected 12 of 58 products excavated from Seongneung. Results showed that the main component used for Geumgu ornaments was tin (Sn), and trace amounts of copper (Cu) and lead (Pb) were also commonly identified. When analyzing the material used to affix the metal fittings, this was found to be glue (膠) made from animal skins, muscle, and bones. This pattern matches that of the GeumguJagi excavated from Paju Hyeeumwonji, and the reason for this could be assumed on the basis of the contents of the 『Cheongonggaemul (天工開物)』 written by Song Ongsung (宋應星) during the Ming Dynasty. At that time, metals such as tin and copper would have been difficult to obtain. 『Xuānhwafengshi Gaolitujing (宣和奉使高麗圖經)』 shows that the use of metal was limited to certain classes; thus, the use of the GeumguJagi seems to have been centered around the royal family.