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http://dx.doi.org/10.17495/easdl.2017.4.27.2.235

Antioxidant Activity and Inhibition Activity against α-Glucosidase and PTP1B of Chrysanthemum indicum L. Extract and Fractions  

Hwang, Sung-Woo (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Cha, Ji-Yun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Lee, Hoon-Yeon (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Kim, Bo-Ram (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Park, Min (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Lee, Bo-Ri (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Moon, Kwang- Hyun (Sunchang Research Institute of Health and Longevity)
Lee, Jeong-Ho (Sunchang Research Institute of Health and Longevity)
Kim, Dae-Geun (Sunchang Research Institute of Health and Longevity)
Kim, Wook (Sunchang Research Institute of Health and Longevity)
Jeong, Kyoung-Ok (Sunchang Research Institute of Health and Longevity)
Lee, Young-Mi (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Publication Information
Journal of the East Asian Society of Dietary Life / v.27, no.2, 2017 , pp. 235-242 More about this Journal
Abstract
In this study, we evaluated the polyphenol content, flavonoid content, antioxidant and biological of 70% EtOH extract and its fractions (dichloromethane, ethyl acetate and water) of the plant Chrysanthemum indicum L. (CI) belonging to family chrysanthemum. antioxidant activity was determined 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and superoxide scavenging activity. The biological activity tests included measurement of ${\alpha}$-glucosidase and PTP1B. The results show that content of polyphenol and flavonoid in 70% EtOH of CI 30.20 mg and 31.58 mg, respectively. Among the different samples, ethyl acetate (EA) fraction showed the highest level of polyphenol (87.87 mg) and flavonoid (65.12 mg). The DPPH, ABTS and superoxide dismutase(SOD) antioxidant assay of EA fraction showed 93.84%, 93.38% and 84.68% inhibition respectively. The EA fraction also showed the highest antioxidant activity compared to 70% EtOH extract and other fractions. EA fraction ($20{\mu}g/mL$) showed the highest 30.29% and 99.24%, respectively compared to other samples. The results of this study show that the EA fraction contained more antioxidant and antidiabetic components than 70% EtOH extract and other fractions. In conclusion, we believe that CI may be useful natural antioxidant and functional material.
Keywords
Chrysanthemum indicum L; antioxidant; DPPH; ${\alpha}$-glucosidase; PTP1B;
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Times Cited By KSCI : 4  (Citation Analysis)
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