• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1184-1191
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• 2011

We attempted to generate a linkage map using Illumina Porcine 60K SNP Beadchip genotypes of the $F_2$ offspring from Korean native pig (KNP) crossed with Yorkshire (YS) pig, and to identify quantitative trait loci (QTL) using the line-cross model. Among the genotype information of the 62,136 SNPs obtained from the high-density SNP analysis, 45,308 SNPs were used to select informative markers with allelic frequencies >0.7 between the KNP (n = 16) and YS (n = 8) F0 animals. Of the selected SNP markers, a final set of 500 SNPs with polymorphic information contents (PIC) values of >0.300 in the $F_2$ groups (n = 252) was used for detection of thirty meat quality-related QTL on chromosomes at the 5% significance level and 10 QTL at the 1% significance level. The QTL for crude protein were detected on SSC2, SSC3, SSC6, SSC9 and SSC12; for intramuscular fat and marbling on SSC2, SSC8, SSC12, SSC14 and SSC18; meat color measurements on SSC1, SSC3, SSC4, SSC5, SSC6, SSC10, SSC11, SSC12, SSC16 and SSC18; water content related measurements in pork were detected on SSC4, SSC6, SSC7, SSC10, SSC12 and SSC14. Additional QTL of pork quality traits such as texture, tenderness and pH were detected on SSC6, SSC12, SSC13 and SSC16. The most important chromosomal region of superior pork quality in KNP compared to YS was identified on SSC12. Our results demonstrated that a QTL linkage map of the $F_2$ design in the pig breed can be generated with a selected data set of high density SNP genotypes. The QTL regions detected in this study will provide useful information for identifying genetic factors related to better pork quality in KNP.

• 한국동물학회지
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• 제31권4호
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• pp.327-333
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• 1988

계절에 따른 초파리 집단내 역위다형 현상의 변동상을 알기 위하여 전주근교의 과수원에서 1983년 1월부터 11월까지 격월로 여섯번 채집한 집단 표본을 분석하였다. 총 1071마리에서 얻은 15개형의 역위는 모두 편동원체역위(paracentric inversion)였으며, 이 중 7개형은 세계형91위(cosmopolitan inversion)였고, 나머지 8개형은 지역형역위(endemic inversion)였다. 개체당 역위의 평균 보유수는 0.639이고 역위를 보유한 개체의 빈도는 0.465였다. 7개의 세계형역위에 대한 표본집단을 분석한 결과 격월 집단간에서 유의한 차이를 보이는 반면 1월과 1 1월 표본간에서는 그 성황이 오히려 비숫해짐으로서 집단내 총역위빈도는 주기적으로 변동하고 있음이 암시된다. 역위와 환경변수와의 다중상관분석에서는 기온, 습도, 강수량이 역위빈도에 유의한 영향을 미치는 것으로 나타났고 역위 중 In(2L)t가 상기한 환경변수 모두에 대하여 유의한 상관관계를 보인 반면, In(3R)C와 In(3R)P는 어느 것과도 상관성을 보이지 않았다. 염색체내 그리고 염색체간의 연관 또는 조합의 검정결과 연관불평형혈상을 1월, 5월, 그리고 7월 표본의 제3염색체에서, 그리고 비균일조합은 1월과 7월 표본에서 역시 제3염색체에서만 산출 되었다. Seasonal changes of inversion frequencies in Chonju 0. melanogaster populations were studied. A total of 1071 males were collected six times with ho months intewal from January through November in 1983. to analyse diploid sets of chromosomes carried by males, each male was mated to several virgin females homoBvgous for cytologically standard sequence in all chromo-somes. From each mating, more than seven FL larvae were selected in random and tested to find chromosomal aberrations. In the present study, 15 different inversions were found and identified to be paracentric only In both second and third chromosomes; seven were cosmopolitan and the rest eight endemic types. The average frequency of inversions was 0.465 and the mean number of inversions carried by a single male was 0.639. The linkage disequilibria were detected between the leK and right arms of third chromosomes from the samples of January, May and Julv, Whereas nonrandom associations appeared also in the third chromosomes only in January and Julv samples. In multiple regression analysis among frequencies of inversions and environ-mental variables it appeared that mean temperature, relative humidity and total precipitation for a month skipped over 30 days before collected affect to change to frequencies of particular inver-sions. With respect to the behavior of inversions in the present samples, it is suggested, with the Friedman's analysis of variance by ranks of inversion frequency orders, that the frequencies of inversions change cyclically year to year.

• 미생물학회지
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• 제30권3호
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• pp.218-224
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• 1992

Carboxydobacteria 의 일산화 탄소 산화에 대한 유전학적 연구를 위해 Pseudomonas caarboxydovorans 에 존재하는 pYK100 plasmid 와 pBR322 를 이용하여 pYK322 (7.2 kb, Ap, Tc) 와 pYK324 (7.2 kb, Ap, Tc) 등 두가지 재조합 plasmid shuttle 백테를 만들고, pYK100와 pACYC184를 이용하여 pYK210(5.2 kb, $CM^{r}$ ), pYK220 (5.2kb,$CM^{r}$ ), pYK230 (5.2 kb, $Cm^{r}$ ), pYK232 (5.2 kb, $CM^{r}$) 등 네가지 shuttle 벡터를 만들었다. 재조합된 벡터들은 보두 대장균에서 안정되게 복제되었다. pYK322 와 pYK220 을 이용한 carboxydobacteria 의 형질전환 실험에서 Bagdasarian 과 Timmis 의 방법 (Curr. Top. Microbiol. Immunol., 96 :47-67, 1982) 을 변형하여 0.2% succinate 가 포함된 무기염류배지에서 지수성장 중기까지 배댜ㄷ한 세균을 이용하고, 형질전환용액의 10 mM RbCI 을 100 mM KCI 로 대체하며, 형질전환용액 처리후 4.deg.C 에서의 방치시간을 12시간으로 하고, DNA첨가휴 45.deg.C 에서 3 분간 heat shock 을 준 경우에 높은 형질전환이 일어났다. 형질전환된 세균으로 부터 형질전환에 사용한 plasmid 를 발견할 수 없었는데, 이는 도입된 plasmid 가 염색체 DNA 에 결합되었기 때문인 것으로 추측된다.

• 미생물학회지
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• 제40권2호
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• pp.81-86
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• 2004

Streptomyces coelicolor의 전 유전체 청보를 분석한 결과(7), 유전자 ID1103135가 코드 하는 open reading frame SCO7697이 phytase[myo-inositol hexakisphosphate phosphohydrolase상동성 (3-6,8,23)]에 유의하게 유사한 것으로 판단되었다. S. coelicolor A3(2)M의 염색체 DNA를 주형으로 PCR 방법으로 SCO7697 전체를 포함하는 DNA 단편을 클로닝하였다. 두 가지의 서로 다른 길이를 갖는 클로닝 된 ID1103135 DNA 단편을 E. coli 발현용 벡터pET728a(+)에 삽입하여,두 종의 재조합 벡터 pET28-SP와 pET28-LP를 얻었다. pET28-SP 와 pET28-LP를 각각 E. coli BL2l에 도입하여, IPTG로 발현 유도된 단백질을 SDS-polyacrylamide 전기영동으로 확인한 결과, 발현은 성공적으로 이루어 졌으나 대부분불용체를 형성하고 분자량은 예상보다 약간 큰 것으로 나타났다. 불용체 형성은 단백질의 불활성화를 수반 함으로서, 배양 온도를 $37^{\circ}C$에서 $30^{\circ}C$로 변화시켜 배양하는 방법으로 발현된 단백질을 가용화 시켰다. 발현된 단백질을 추출하여 조추출물 또는 정제한 상태로 phytase활성을 측청하였으나 효소활성은 관찰할 수 없었다. 대장균 시스템에서의 발현이 효소 활성의 소실을 초래했을 가능성이 있으므로, ID1103135 유전자를 자신의 promoter를 함유하도록 PCR 클로닝하여, E. coli - Streptomyces의 shuttle vector인 pWHM3에 삽입하고, 이를 방선균 호스트인 S. lividans에 도입하였다. 형질 전환체의 세포조추출액 및 세포배양액의 phytase 활성을 측청하였으나, 역시 활성을 확인할수 없었다. 이와 같은 결과는 SCO7697이 아주 높은 확률(E value; $6e^{-89}$)로 phytase일 것으로 annotation 되었으나, 실제는 이와는 다른 기능을 함유하고 있음을 시사하고 있다.

• Journal of Preventive Medicine and Public Health
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• 제31권4호
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• pp.616-627
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• 1998

In order to evaluate the cytogenetic hazard among hospital workers potentially exposed to low dose of radiation, the analysis of chromosome aberrations(CA) and sister chromatid exchanges(SCE) in lymphocytes were performed in 79 hospital workers and 79 non-exposed workers. The mean frequency of chromosomal exchange and deletion(respectively, $0.20\times10^{-2}/cell\;and\;0.39\times10^{-2}/cell$) in the exposed group were significantly higher than those$(0.07\times10^{-2}/cell\;and\;0.23\times10^{-2}/cell)$ in control group. The frequency of sister chromatid exchanges was 5.04/cell in the control vs. 6.57/cell in the exposed group. There were also significant differences in the mean frequencies of CA and SCE adjusted for age, sex, smoking, drinking between two groups. There were no evidence of significant increase of CA and SCE according to the department or duration of employment. But the frequency of cells having chromosome aberration was significantly higher in the exposed group than in the control group related to duration of employment. There was no dose-effect relationship between the cumulative doses and the frequency of CA and SCE. But in the case of last 1 yr cumulative dose, there were evidence of significant dose-dependant increase of chromosome type CA and percentage of cells with aberration. The result suggest that there is cytogenetic hazard in risk group like hospital workers handling low dose radiation. And the analysis CA and SCE are useful biological indicators for the exposure of low dose level of radiation.

• 한국작물학회지
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• 제37권5호
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• pp.476-485
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• 1992

1. 옥수수의 자식계 9계통에 화학적 돌연변이 유기제를 처리하여 얻어진 M$_1$, 식물들은 형태적으로 왜성화, 백색화등 다양한 변이체로 나타났고 생식기관의 변이는 자성개체, 웅성개체, 자웅동화, 다수성들을 보였고 부임개체들이 증가하였다. 2. mutagen의 종자침지처리후 이삭에 수분방지대를 씌웠을때는 3.9-11.2%의 이삭착립율이 나타났으나 이삭당 성숙립수는 3.8-4.3게에 불과하였다. 한편 mutagen의 이삭주입처리에서는 5.1-10%의 착립이삭율과 이삭당 성숙립수는 1.7-6.3이었고 자연수분후 4시간만에 웅사를 절단하였던 이삭들은 착립이삭수가 27%로 증가하였다. 3. Mutagen처리와 수분방지처리에서 형성된 종자들의 조직학적 관찰은 aposporous apomictic reproduction이나 adventitious embryony의 가능성을 보여주었고 M2식물의 근단염색체를 조사하였을때 모두 2배체로서 확인되었다. 4. 무배합생식성 종자에서 출현된 식물들은 초장의 변이계수가 4-6%로 비교적 낮았고 외부형태상의 균일성을 보여주었다. 그러나 이들 무배합생식성과 결부된 표식형질을 발견할수는 없었다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.844-854
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• 2005

We constructed a defined physical and genetic map of H. pylori strain 51, previously isolated from a Korean patient with a duodenal ulcer, by combining a restriction analysis by pulse-field gel electrophoresis with the construction of a BAC library. A Notl-digest of H. pylori strain 51 genome yielded seven fragments, from which the genomic size was estimated to be 1,698$\pm$24 kb. The BAC library was constructed from 50 to 200 kb fragments of HindIII-digested genomic DNA. From 700 BAC clones, an ordered overlapping maxi-set of 82 BAC clones was assembled that covered the entire genome. The positions of 15 genes were localized in the strain 51 genome with 4-22 kb of resolution and were compared with their orthologues in strain 26695 and strain J99. The arrangement of the 15 genes was identical in strain 51 and strain J99, except for flaA and hpaA. The plasticity zone of strain 51, like that of strain J99, was located in the single region, and was shorter than those of strain 26695 and strain J99. The strain 51 plasticity zone consisted of ORFs common only to strain 51 and J99 or to strain 51 and 26695, as well as strain 51-specific ORFs. Three genetic translocations and/or inversions were found between orthologue ORFs in strain 51 and strain J99. These results show that the chromosomal organization of strain 51 differs from Western strains such as strain 26695 and strain J99.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.880-886
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• 2005

Using carotenoid genes of Erwinia herbicola, metabolic engineering was carried out for lycopene production with the pAC-LYCO4 plasmid, which was composed of a chromosomal DNA fragment of E. herbicola containing the crtE, crtB, and crtI genes under the control of the tetracycline promoter and the ipi gene of Haematococcus pluvialis with the trc promoter. Plasmid pAC-LYCm4 was constructed for efficient expression of the four exogenous genes using a strong RBS sequence and the same tetracycline promoter. The optimized expression construct of pAC-LYCm4 increased Iycopene production three times as compared with pAC-LYCO4. pAC-LYCm5 containing ispA behind the four exogenous genes was constructed. There was no significant difference in Iycopene production and cell growth between pAC-LYCm4 and pAC-LYCm5. FPP synthase encoded by ispA was not rate-limiting for Iycopene production. Each gene of crtE, crtB, crtI, and ipi was overexpressed, using pBAD-crtE, pBAD-crtIB, and pBAD-ipiHPI, in addition to their expression from pAC-LYCm4. However, there was no increase oflycopene production with the additional overexpression of each exogenous gene. The four exogenous genes appeared to be not rate-limiting in cells harboring pAC-LYCm4. When pDdxs, pBAD24 containing dxs, was introduced into cells harboring lycopene synthetic plasmids, lycopene production of pAC-LYCO4, pAC-LYCm4, and pAC-LYCm5 was increased by 4.7-, 2.2-, and 2.2-fold, respectively. Lycopene production of pBAD-DXm4 containing crtE, crtB, crtI, ipi, and dxs was 5.2 mg/g dry cell weight with $0.2\%$ arabinose, which was 8.7-fold higher than that of the initial strain with pAC-LYC04. Therefore, the present study showed that proper regulation of a metabolically engineered pathway is important for Iycopene production.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.436-439
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• 1989

고온, 호알칼리성 Bacillus속 K-17 균주에서 $\beta$-xylosidase 유전자를 pBR322를 벡터로 이용하여 클로닝시켰다. p-Nitrophenyl-$\beta$-xylopyranoside를 함유하는 LB 한천배지에서 노란색을 형성하는 대장균 형질전환주에서 재조합 플라스미드 pAX278을 분리하였으며, 본 pAX278은 pBR322와 고온, 호알칼리성 Bacillus K-17 균주 염색체 DNA의 5.0 kb HindIII절편으로 구성되어 있었다. Biotin으로 로식된 pAX278을 probe로 하여 상동성 시험을 하여 본 결과, pAX278에 존재하는 5.0 kb HindIII 절편은 Bacillus K-17 균주의 염색체 DNA HindIII 절편 중에서 5.0 kb 분만 아니라 0.9 kb 절편과도 상동성이 있었다. pAX278의 5.0 kb 절편을 pGR71에 연결시켜 B. subtilis에서도 발현시켰다. pAX278을 가지는 E. coli 균주가 생성하는 $\beta$-xylosidase는 균체외에 존재하였으며 그 효소학적 성질은 Bacillus속 K-17의 $\beta$-xylosidase와 동일하였다.

• 한국가축번식학회지
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• 제13권2호
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• pp.113-120
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• 1989

In order to determine the sex of preimplantation embryos prior to transfer in cattle, a series of experiments were carried out using 45 Holstein donor cows to examine the ovarian response on the gonadotropin and PGF2${\alpha}$, and the morphology of fresh embryos or frozen/thawed embryos after deep freezing at -196$^{\circ}C$. The sexing of embryos treated with the medium containing H-Y antiserum(10%, v/v) and FITC anti-mouse IgG(10%, v/v) were analysed by chromosomal analysis, and the sex of the embryos which survived were ascertain after delivering the pups. The results obtained were summarized as follows ; 1. The average number of developed follicle and corpus luteum per cow were 13.5 and 8.1, and the ovalation rate was 60.1%. 2. Of 220-ova recovered, 75(34.1%) were morula and 91(41.4%) were blastocyst, and the morphological normal and abnormal rate of ova recovered were 75.5% and 24.5%, respectively. 3. Of 39 frozen/thawed embryos, the scores of normal morula and blastocyst, after thawing were 79.2%(19/24) and 73.3%(11/15). The average rate of frozen/thawed embryos which appeared morphologically normal post thawing was 76.9%(30/39). 4. The sex ratio was measured using the embryos treated with immunofluorescence assay to examine the relationship between embryo developmental stage, sex ratio of morula stage embryo was 42.2%(19/45) fluorescing and 57.8%(26/45) non-fluorescing, on the other hand, the ratio switched to 46.8%(29/62) fluorescing and 53.2%(33/62) non-fluorescing embryo in blastocyst stage. The sex ratio was also measured between fresh and frozen/thawed embryos, fresh and frozen/thawed treated embryos were indicated 45.8%(38/83) fluorescing, 54.2%(45/83) non-fluorescing and 41.7%(10/24) fluorescing, 58.3%(14/24) non-fluorescing. This trend indicated the approximal sex ratio was 1 : 1. 5. The result of karyotype test showed the successful rate of sexing embryo is fluorescing and non-fluorescing was 21.2%(7/33) and 29.6%(8/27). The female to male ratio within 33 fluorescing was 28.6 : 71.4, and the ratio of 27 non-fluorescing embryos was 87.7 : 12.5. 6. Of the embryo transferred after assignment of H-Y phenotype, five of the fluorescing embryos survived to term, all was males. Whereas six non-fluorescing embryos also survived to term and the sexes of the calves were 1 male 5 female.