• 한국약용작물학회지
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• 제21권6호
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• pp.486-492
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• 2013

This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at $85^{\circ}C$ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as $27.27{\pm}2.27%$, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of $10.55{\pm}0.24%$. The quantitative determination methods of calycosin-7-O-${\beta}$-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column ($4.6{\times}250mm$, $5{\mu}m$) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of $0.8mLmin^{-1}$ and a detection wavelength of 230nm. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-${\beta}$-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as $1,700.3{\pm}30.4$ and $443.6{\pm}8.4{\mu}g-1$, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at $85^{\circ}C$ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

• 대한금속재료학회지
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• 제56권12호
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• pp.885-892
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• 2018

Molybdenum disulfide ($MoS_2$) based electrocatalysts have been proposed as substitutes for platinum group metal (PGM) based electrocatalyst to hydrogen evolution reaction (HER) in water electrolysis. Here, we studied $MoS_2/CNFs$ hybrid catalyst prepared by electrospinning method with heat treatment for polymer electrolyte membrane(PEM) water electrolysis to improve the HER activity. The physicochemical and electrochemical properties such as average diameter, crystalline properties, electrocatalitic activity for HER of synthesized $MoS_2/CNFs$ were investigated by the Scanning Electron Microscope (SEM), X-ray Diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), Transmission Electron Microscopy (TEM), Raman Spectroscopy (Raman) and Linear Sweep Voltammetry (LSV). The as spun ATTM/PVP nanofibers were prepared by sol-gel and electrospinning method. Subsequently, the $MoS_2/CNFs$ was dereived from reduction heat treatment of ATTM at the ATTM/PVP nanofibers and carbonization heat treatment. Synthesized $MoS_2/CNFs$ electrocatalyst had an average diameter of $179{\pm}30nm$. We confirmed that the $MoS_2$ layers in $MoS_2/CNF$ electrocatalyst consist of 3~4 layers from the Raman results. In addition, We confirmed that the $MoS_2$ layers in $MoS_2/CNF$ catalyst consist of 7.47% octahedral 1T phase $MoS_2$, 63.77% trigonal prismatic 2H phase $MoS_2$ with 28.75% $MoO_3$ through the XRD, Raman and XPS results. It was shown that $MoS_2/CNFs$ had the overpotential of 0.278 V at $10mA/cm^2$ and tafel slope of 74.8 mV/dec in 0.5 M sulfuric acid ($H_2SO_4$) electrolyte.

• 농약과학회지
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• 제17권4호
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• pp.473-477
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• 2013

본 연구는 병해충 관리용 유기농업자재 허용물질로 지정된 식물추출물 중 님 추출물과 고삼추출물, 데리스 추출물 원제의 독성 평가를 위해 꿀벌에 대한 급성 접촉 및 섭식독성시험을 수행하였다. 급성접촉독성시험결과 님 추출물 2종과 데리스 추출물 2종은 $LD_{50}$이 100 ${\mu}g/bee$ 이상으로 독성이 매우 낮았고 고삼 추출물은 2종 모두 $LD_{50}$(48h)이 1.7 ${\mu}g/bee$로 매우 강한 독성을 보였다. 급성섭식독성시험결과 님 추출물 2종과 데리스 추출물 2종은 $LD_{50}$이 100 ${\mu}g/bee$ 이상으로 독성이 매우 낮았고 고삼 추출물A는 $LD_{50}$(48h)이 1.7 ${\mu}g/bee$, 고삼 추출물B는 $LD_{50}$(48h)이 0.3 ${\mu}g/bee$으로 모두 매우 강한 독성이 있는 것으로 평가되었다.

• 농약과학회지
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• 제17권4호
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• pp.478-481
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• 2013

본 연구는 병해충 관리용 유기농업자재 허용물질로 지정된 식물추출물 중 님 추출물(미얀마산, 인도산)과 고삼추출물(중국산)의 독성 평가를 위해 피부자극성과 안점막자극성시험을 수행하였다. 시험결과, 원산지가 다른 님 추출물 2종은 모두 피부자극성과 안점막자극성이 없는 것으로 평가되었고, 고삼 추출물은 피부자극성은 없었고 안점막자극성이 강도로 나타났으나 시간이 경과함에 따라 자극이 경감하는 것으로 평가됐다. 따라서 안점막자극성이 강한 것으로 평가된 고삼 추출물을 유기농업자재로 사용할 때 농작업자에 대한 안전대책이 필요한 것으로 판단된다.

• Journal of Korean Neurosurgical Society
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• 제61권2호
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• pp.127-166
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• 2018

Despite advancements in treating ruptured cerebral aneurysms, an aneurysmal subarachnoid hemorrhage (aSAH) is still a grave cerebrovascular disease associated with a high rate of morbidity and mortality. Based on the literature published to date, world-wide academic and governmental committees have developed clinical practice guidelines (CPGs) to propose standards for disease management in order to achieve the best treatment outcomes for aSAHs. In 2013, the Korean Society of Cerebrovascular Surgeons issued a Korean version of the CPGs for aSAHs. The group researched all articles and major foreign CPGs published in English until December 2015 using several search engines. Based on these articles, levels of evidence and grades of recommendations were determined by our society as well as by other related Quality Control Committees from neurointervention, neurology and rehabilitation medicine. The Korean version of the CPGs for aSAHs includes risk factors, diagnosis, initial management, medical and surgical management to prevent rebleeding, management of delayed cerebral ischemia and vasospasm, treatment of hydrocephalus, treatment of medical complications and early rehabilitation. The CPGs are not the absolute standard but are the present reference as the evidence is still incomplete, each environment of clinical practice is different, and there is a high probability of variation in the current recommendations. The CPGs will be useful in the fields of clinical practice and research.

• 한국약용작물학회지
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• 제19권5호
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• pp.334-340
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• 2011

The study was done to investigate the effects of the extracts from the different parts of Lythrum salicaria (LS) on liver protective activities in chronically alcohol-treated rats. SD male rats except normal animals were administrated with alcohol ($30m{\ell}$ of 30%~40% ethanol/kg/day) and the extracts (300 mg/kg/day) for 10 weeks. Chronic alcohol administration decreased body weight, high density lipoprotein (HDL)-cholesterol and the reduced form-glutathione (GSH), whereas increased the ethanol content, glutamic-oxaloacetic transaminase (GOT), total cholesterol, low density lipoprotein (LDL)- cholesterol, triglyceride in blood/serum and the ratio of the oxidized form of glutathione (GSSG) and total GSH (GSSG/total GSH) in liver tissue. Groups treated with the extracts of leaf, root and stem, showed decrease in GOT, total cholesterol and GSSG/total GSH and increase in hepatic aldehyde dehydrogenase (ALDH), total GSH and serum albumin. Administration with the root extract of LS decreased blood ethanol content compared with the other part extracts. But, serum triglyceride values in rats treated with root and stem extract were higher than that of the negative control animals. Flower extract-fed group showed decrease in body weight and serum triglyceride, but increase in the ratio of GOT and glutamic-pyruvic transaminase (GPT), and GSSG/total GSH. From the results, we conclude that the extracts of root and leaf among the plant parts of LS might be useful for the amelioration of the chronic alcohol-induced liver demage of rat.

• Journal of Korean Neurosurgical Society
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• 제49권6호
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• pp.355-358
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• 2011

Objective : Subdural hygroma (SDG) is a complication occurring after head trauma that may occur secondary to decompressive craniectomy (DC). However, the mechanism underlying SDG formation is not fully understood. Also, the relationship between the operative technique of DC or the decompressive effect and the occurrence and pathophysiology of SDG has not been clarified. Purpose of this study was to investigate the risk factors of SDG after DC in our series. Methods : From January 2004 to December 2008, DC was performed in 85 patients who suffered from traumatic brain injury. We retrospectively reviewed the clinical and radiological features. For comparative analysis, we divided the patients into 2 groups : one group with SDG after craniectomy (19 patients; 28.4% of the total sample), the other group without SDG (48 patients; 71.6%). The risk factors for developing SDG were then analyzed. Results : The mean Glasgow Outcome Scale (GOS) scores at discharge of the groups with and without SDG were 2.8 and 3.1, respectively (p<0.0001). Analysis of radiological factors showed that a midline shift in excess of 5 mm on CT scans was present in 19 patients (100%) in the group with SDG and in 32 patients (66.7%) in the group without SDG (p<0.05). An accompanying subarachnoid hemorrhage (SAH) was seen in 17 patients (89.5%) in the group with SDG and in 29 patients (60.4%) in the group without SDG (p<0.05). Delayed hydrocephalus accompanied these findings in 10 patients (52.6%) in the group with SDG, versus 5 patients (10.4%) in the group without SDG (p<0.05). On CT, compression of basal cisterns was observed in 14 members (73.7%) in the group with SDG and in 18 members of the group without SDG (37.5%) (p<0.007). Furthermore, tearing of the arachnoid membrane, as observed on CT, was more common in all patients in the group with SDG (100%) than in the group without SDG (31 patients; 64.6%) (p<0.05). Conclusion : GOS showed statistically significant difference in the clinical risk factors for SDG between the group with SDG and the group without SDG. Analysis of radiological factors indicated that a midline shifting exceeding 5 mm, SAH, delayed hydrocephalus, compression of basal cisterns, and tearing of the arachnoid membrane were significantly more common in patients with SDG.

• 농약과학회지
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• 제11권4호
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• pp.222-229
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• 2007

천연기능성물질을 함유한 농산물을 활용하여 국민건강을 보호할 수 있는 방안을 모색하기 위해 항산화 및 항암 효과가 있는 것으로 알려진 양파를 이용하여 수컷 rat에서 농약독성 경감효과를 시험하였다. 6주간 양파즙액을 투여한 후 chlorprifos 0, 2, $4\;mg\;kg^{-1}$을 복강내 투여하였다. 간, 폐, 혈청 중 항산화효소인 SOD의 변화를 조사한 결과 양파즙액 투여 후 chlorprifos를 투여한 군이 chlorpyrifos 단독 투여군에 비해 간과 폐의 SOD 활성이 높게 나타나는 것으로 나타나 항산화기능이 증가함을 볼 수 있었다. 혈청 중 BUN/Creatinine ratio를 조사한 결과 양파즙액 투여 후 chlorpyrifos를 투여한 군이 단독 투여군에 비해 수치가 낮게 나타나 신장독성을 감소시킬 수 있을 것으로 판단된다.

• 한국약용작물학회지
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• 제28권1호
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• pp.9-20
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• 2020

Background: Although a number of Panax ginseng cultivars have been developed by Korean researchers in recent years, there has been insufficient analysis of their beneficial properties. In this study, we sought to identify useful ginseng varieties as functional materials. Methods and Results: We evaluated effects of root extracts of 10 ginseng cultivars (Cheongsun; CS, Chunpoong; CP, Gopoong; GP, Gumpoong; GMP, K1, Sunhyang; SH, Sunone; SO, Sunpoong; SP, Sunun; SU and Yunpoong; YP) against the inhibitory effects of nitric oxide (NO) and reactive oxygen species (ROS) production in mouse brain microglial BV2 cells, as well as the binding of N-methyl-D-aspartate receptor (NMDAR), a marker related to memory. Ginsenosides, such as 20 (S)-protopanaxadiols (PPDs), including ginsenoside-Rb1, -Rb2, -Rb3, -Rc, -Rd, and - Rg3 and 20 (S)-protopanaxatriols (PPTs) including -Re, -Rg1, and -Rg2 were analyzed by HPLC. We observed that the cultivar GMP showed the highest inhibitory effect (60.8%) against NO production at 20 ㎍/㎖. Those cultivars showing the significantly highest inhibition effects against ROS at 20 ㎍/㎖ were K1 (57.3%), SP (54.5%), YP (53.1%), CP (51.7%), CS (50.9%) and SH (49.6%). At 50 ㎍/㎖, K1 showed the most potent inhibitory effect (51.2%) on NMDAR binding. The total phenol content of SH (1.89 mg/g) and K1 (1.73 mg/g) were higher than those of the other cultivars, whereas in terms of PD/PT ratios, the values of CP (0.98), K1 (1.05) and SO (1.05) were lower than those of the other cultivars. On the basis of correlation coefficient (0.7064) between NMDAR inhibition and ONOO^- scavenging activity. Conclusions: The findings of this study indicate that the cultivars K1 and SH could be useful ginseng resources as functional materials with favorable cognition-improving and antioxidative properties.

• Journal of Acupuncture Research
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• 제24권6호
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• pp.37-44
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• 2007

Objectives : The purpose of this study is to find out the effects of Chuna treatment on neck pain caused by traffic accidents. Methods : The 10 patients were divided into 2 groups, with Group A treated with Acupuncture and Chuna, while group B was treated with Acupuncture only. We measured the Visual Analog Scale(VAS), Pain Disability Index(PDI) and Pressure Pain Thresholds before and after treatment in each group. The statistical analysis was performed by using a Mann-Whitney U test and Wilcoxon signed rank test. Results : 1. Group A showed significant improvements in VAS, PDI and Pressure Pain Thresholds(p<0.05). 2. Group B was significantly improved in VAS(p<0.05). However, there was no statistical significance in PDI and Pressure Pain Thresholds(p>0.05). 3. There was no statistical significance between Group A and Group B before and after treatment. Conclusions : These results imply that Chuna treatment with Acupuncture would beeffective and useful on the neck pain caused by traffic accidents.