• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.166-169
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• 1999

The experiment was conducted to find the changes of early growth and chemical components such as chlorophyll content, superoxide dismutase(SOD) activity, free proline content on the different manganese chloride concentration(2,500, 3,500 and 4,500ppm) in rice seedling. Root growth was decreased in highest concentration, 4,500 ppm of Mn compared with the control and germination rate was also decreased 43% at 4,500 ppm of Mn. Chlorophyll content was decreased at Mn 4,500ppm with 1.16mg. Free proline content at 3 day after germination in Mn 4,500ppm was highest relative to the other manganese chloride concentrations. SOD activity was gradually increased as manganese chloride concentration was increased. As a result, it was suggested that an increment of free proline and SOD activity results from the higher manganese chloride concentration.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.646-651
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• 2007

This investigation was performed to study the influence of Cd and nitrate on growth, and chlorophyll and photosynthetic enzymes in seedling of tobacco. Growth inhibition by Cd was not recovered by nitrate. Chlorophyll levels were reduced by Cd. The combination of Cd and low concentration of nitrate decreased the chlorophyll content compared to that in plants exposed only to Cd. Activity and content of rubisco at Cd treatment was significantly lesser than in plants receiving no treatment, These data suggest that rubisco activity was associated with an amount of rubisco protein, and that the activation and synthesis of rubisco is inhibited by Cd. Both the activity and content of rubisco decreased by Cd were more decreased by nitrate. A similar change pattern was also observed in activity and content of rubisco activase. These results suggest that Cd- and nitrate-induced changes of rubisco could be correlated with rubisco activase, and that nitrate was concerned in not only the activation and synthesis of rubisco directly, but also rubisco activase leading to a large change in rubisco.

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.167-174
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• 2006

This study was initiated to investigate the effect of chitosan on creeping bentgrass growth. Chitosan was applied several times in dilution of 300, 500 and 800 times at ten-day intervals after transplanting. Such growth characteristics as leaf length, root length, numbers of leaves, fresh weight and dry weight and chlorophyll content were observed. Treatment of 500 times diluted chitosan resulted in the longest root length, being 31.5cm while the control the shortest root of 25.1cm. Leaf numbers were 27.9 and 45.5, respectively for the control and the 300 times treatment. The highest chlorophyll content was associated with treatment of 300 times diluted chitosan and the lowest one with the control, resulting in 11.9 and $18.4mg/100cm^2$, respectively. We found that leaf number, chlorophyll content, fresh and dry weight were higher in the treatment of 500 times than the other treatments.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.6
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• pp.180-189
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• 2012

This study was carried out to suggest an experiment based for selecting Sedum, which can adapt well with heat tolerance in extensive green roof system. The heat tolerance of Sedum subject to laboratory high temperature treatment and heat processing time were evaluated using electrolyte leakage, chlorophyll content and regrowth test, and the relation between soil water content and heat tolerance were researched. Logistic model of nonlinear regression analysis was used to evaluate the lethal temperatures that were predicted with the range of $45.0{\sim}48.1^{\circ}C$(soil water content 5%), $47.5{\sim}49.3^{\circ}C$(10%), $48.6{\sim}52.8^{\circ}C$(15%) in 6-hours high-temperature treatment. The higher the soil water content, the stronger the heat resistance property of Sedum. there is. The higher the treatment temperature, the lower the chlorophyll content, and the less the soil water content, the faster the chlorophyll decomposition. The order of hot-temperature resistance was S. reflexum>S. takevimense>S. middendorffianum>S. album>S. sieboldii>S. spurium when soil water content was 5%. The order of hot-temperature resistance was S. album>S. reflexum>S. spurium>S. takevimense>S. middendorffianum>S. sieboldii when soil water content was 15%. The more of soil water content, S. album, S. reflexum, S. spurium had stronger tolerant of hot temperature. These results were consistent with those from the regrowth test and the heat tolerance tested by electrolyte leakage evaluation.

• Journal of Forest and Environmental Science
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• v.32 no.2
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• pp.205-211
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• 2016

Leaf chlorophyll content provides valuable information about physiological status of plants. However, fewer studies have investigated the difference in chlorophyll concentration in leaves of tropical afforestation tree species. Therefore, this study examines the difference in foliar chlorophyll contents of six tropical afforestation tree species namely: Tectona grandis, Pentaclethra macrophylla, Piptademiastrum africanum, Azadirachta indica, Brachystegia eurycoma and Gmelina arborea found in the relict forest in Umudike, South east, Nigeria. A single factor experiment in a completely randomised design in three replicates was employed to analyse the rate of leaf chlorophyll contents. Fisher's least significant different was used to test for significance in mean difference in foliar chlorophyll contents between tree species at 95% confidence interval using analysis of variance. The results of this study showed a significant difference in foliar chlorophyll concentration between the tree species with Tectona grandis having a higher chlorophyll concentration than other trees this could be as a result of its higher vegetative activity which increases its primary productivity followed by Pentaclethra macrophylla while Azadirachta indica having least the chlorophyll concentration. The study further revealed that other indigenous tree species like Piptademiastrum africanum and Brachystegia eurycoma have higher chlorophyll concentration. Further studies should be carry out to examine factors that have contributed informed the differences in the chlorophyll concentration of these trees species, thus this would broaden the understanding of their physiological status and equally encourage there conservation.

• Journal of Ecology and Environment
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• v.34 no.3
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• pp.251-257
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• 2011

The purpose of this study was to investigate the acclimation responses of Tamarix chinensis to cold stress. We evaluated the acclimation responses by measuring biomass, daily elongation rate, chlorophyll content, and total soluble carbohydrate content. The plant samples comprised leaves from seedlings of 2 different ages (8 and 12 weeks); the leaves were collected 0, 2, and 4 weeks after cold treatment. We found that the cold-treated samples showed reduced daily elongation rates and chlorophyll content. Further, these samples showed more than 8-fold increase in the total soluble carbohydrate content. However, the seedling ages did not have a significant influence on the growth of cold-treated seedlings. On the basis of these findings, we can conclude that T. chinensis seedlings aged less than 1 year old show acclimation to cold stress by accumulating soluble carbohydrates. This study may help us understand how T. chinensis seedlings acclimatize to their first cold season.

• Journal of Plant Biotechnology
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• v.50
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• pp.127-136
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• 2023

Water scarcity decreases the rate of photosynthesis and, consequently, the yield of banana plants (Musa spp). In this study, transcriptome analysis was performed to identify photosynthesis-related genes in banana plants and determine their expression profiles under water stress conditions. Banana plantlets were in vitro cultured on Murashige and Skoog agar medium with and without 10% polyethylene glycol and marked as BP10 and BK. Chlorophyll contents in the plant shoots were determined spectrophotometrically. Two cDNA libraries generated from BK and BP10 plantlets, respectively, were used as the reference for transcriptome data. Gene ontology (GO) enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and visualized using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway prediction. Morphological observations indicated that water deficiency caused chlorosis and reduced the shoot chlorophyll content of banana plantlets. GO enrichment identified 52 photosynthesis-related genes that were affected by water stress. KEGG visualization revealed the pathways related to the 52 photosynthesisr-elated genes and their allocations in four GO terms. Four, 12, 15, and 21 genes were related to chlorophyll biosynthesis, the Calvin cycle, the photosynthetic electron transfer chain, and the light-harvesting complex, respectively. Differentially expressed gene (DEG) analysis using DESeq revealed that 45 genes were down-regulated, whereas seven genes were up-regulated. Four of the down-regulated genes were responsible for chlorophyll biosynthesis and appeared to cause the decrease in the banana leaf chlorophyll content. Among the annotated DEGs, MaPNDO, MaPSAL, and MaFEDA were selected and validated using quantitative real-time PCR.

• ALGAE
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• v.18 no.2
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• pp.129-134
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• 2003

In order to examine the effects of deep seawater (mesopelagic water in the broad sense) on the growth of macroalgae, the growth and nutrient uptake (nitrate and phosphate) of Ulva sp. (Ulvophyceae, Chlorophyta) were investigated by cultivation in deep seawater (taken from 687 m depth at Yaizu, central Japan, in August 2001), surface seawater (taken from 24 m depth), and a combination of the two. Culture experiments were carried out in a continuous water supply system and an intermittent water supply system, in which aerated 500-mL flasks with 4 discs of Ulva sp. (cut sections of ca. 2 $cm_2$) were cultured at 20$^{\circ}C$ water temperature, 100 $\mu$mol photons $m^{-2}{\cdot}s^{-1}$ light intensity, and a 14:10 light:dark cycle. Nutrient uptake by Ulva sp. was high in all seawater media in both culture systems. The frond area, dry weight, chlorophyll a content, dry weight per unit area, and chlorophyll a content per unit area of Ulva sp. at the end of the experimental period were the highest in deep seawater and the lowest in surface seawater in both culture systems. These values, except for dry weight per unit area and chlorophyll a content per unit area, for each seawater media in the intermittent water supply system were higher than those in the continuous water supply system. We conclude that not only deep seawater as the culture medium but also the seawater supply system is important for effective cultivation of macroalgae.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.3
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• pp.67-72
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• 2005

For the two species of native fern that have high development potential for interior landscape, Rumohra aristata and Cyclosorus dentatus, the physiological and growth characteries as nitrogen application rate such as $NaNO_3$ 0.0mg/L, $NaNO_3$ 0.5mg/L, $NaNO_3$5.0mg/L, $NaNO_3$ 50mg/L and $NaNO_3$ 500mg/L were surveyed. 1. A significant changes of Rumohra aristata, in top growth was not distinctive. However, as nitrogen application rate increased, the numbers of leaves declined and the content of chlorophyll and the number of wilted leaves increased. 2. As nitrogen application rate increased, the top growth of Cyclosorus dentatus and the content of chlorophyll was increased. However, its overall, ornamental value was reduced due to the rising number of wilted leaves and the declining number of new leaves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.2
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• pp.134-138
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• 2001

This experiment evaluates wintering ability to maintain green color of lawn grasses during winter and investigates the effects of top dressing of fertilizer on improving green color during regrowth. Kentucky blue-grass could maintain green color and leaf chlorophyll content better than tall fescue and creeping bentgrass in winter. All three grasses in this experiment have shown the excellent wintering ability. In enhancing the recovery of green color at the early stage of regrowth, the mulching effect with rice straw was highly significant for creeping bentgrass. Green color recovery in grasses during its regrowth was better at the top dressing plots than at the plots without top dressing, but when fertilizer application levels were increased, green color in lawn grass did not significantly change. Although green color in tall fescue, Kentucky bluegrass, and Korean lawngrass could be maintained during summer, the green color of creeping bentgrass is reduced significantly with high temperature. Top dressing after winter and mowing improved leaf chlorophyll content and green color in tail fescue and Kentucky bluegrass significantly. However, Korean lawngrass did not respond significantly with increased levels of fertilizer.