Effects of Manganese Chloride on Chlorophyll, Free Proline and SOD Activity of Rice Seedling

염화망간 처리가 벼 유묘의 엽록소, 유리 Proline 및 SOD 활성에 미치는 영향

  • Published : 1999.06.01

Abstract

The experiment was conducted to find the changes of early growth and chemical components such as chlorophyll content, superoxide dismutase(SOD) activity, free proline content on the different manganese chloride concentration(2,500, 3,500 and 4,500ppm) in rice seedling. Root growth was decreased in highest concentration, 4,500 ppm of Mn compared with the control and germination rate was also decreased 43% at 4,500 ppm of Mn. Chlorophyll content was decreased at Mn 4,500ppm with 1.16mg. Free proline content at 3 day after germination in Mn 4,500ppm was highest relative to the other manganese chloride concentrations. SOD activity was gradually increased as manganese chloride concentration was increased. As a result, it was suggested that an increment of free proline and SOD activity results from the higher manganese chloride concentration.

본 실험은 볍씨에 염화망간의 농도를 2500, 3500 및 4,500ppm을 처리하여 벼의 초기생육에 대한 생육반응, 엽록소 함량, 유리 proline 및 SOD활성변화를 자포니카형인 일품벼를 실험재료로하여 실험한 결과를 요약하면 다음과 같다. 1. 근장은 무처리보다 망간농도 4,500ppm에서는 0.3cm로 망간농도가 증가할수록 뿌리 생장이 억제되었다. 2. 발아율은 망간농도 3,500ppm에서 68%였으나 망간농도 4,500ppm에서 는 43%로 가장 낮았다. 3, 엽록소 함량은 무처리보다 고농도인 4,500ppm 에서 1.16mg으로 가장 낮았다. 4. 유리 proline 함량은 무처리에서는 발아 후 3일과 8일에서 큰 차이를 보이지 않았고 발아 후 3일째 망간농도2,500ppm과 4,500ppm에서 각각 3,286$\mu$mole과 5,872$\mu$mole로 망간농도가 높아질수록 유리 proline 함량이 증가하였다. 5. SOD 활성은 발아 후 2일째 무처리가 762EU인 것에 비해 망간농도 4,500ppm에서는 1,693EU로 가장 높았다.

Keywords

References

  1. Physiol. Plant. v.72 Activities of Cu-containing proteins in Cu-depleted pea leaves Ayala, M. B.;G. Sandmann
  2. Plant and Soil. v.39 Rapid determination of free proline for water-stress studies Bates, L.S.
  3. Laboratory manual for physiological studies of rice v.3 James H. Cock;Kwanchai A. Gomez;Shouichi Yoshida;Douglas A. Forno
  4. Korean J. Crop Sci. v.41 no.4 Effects of seed soak-ing of kinetin on alleviating copper toxicity during germination in rice Kim, Sang Kuk;Sang Chul Lee;Gi Gun Min;Seong Phil Lee;Boo Sull Choi
  5. Mineral nutrition of higher plants Marschner H.
  6. J. Biol. Chem. v.244 no.22 Superoxide dismu-tase : an enzymatic function for erythrocuprein (hemocuprein) McCord, J. M.;I. Fridovich