Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.127-136
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  • 2023
  • /
  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Chlorophyll contents and expression profiles of photosynthesis-related genes in water-stressed banana plantlets

  • Sri Nanan Widiyanto (School of Life Sciences and Technology, Institut Teknologi Bandung) ;
  • Syahril Sulaiman (School of Life Sciences and Technology, Institut Teknologi Bandung) ;
  • Simon Duve (School of Life Sciences and Technology, Institut Teknologi Bandung) ;
  • Erly Marwani (School of Life Sciences and Technology, Institut Teknologi Bandung) ;
  • Husna Nugrahapraja (School of Life Sciences and Technology, Institut Teknologi Bandung) ;
  • Diky Setya Diningrat (Department of Biology, Faculty of Mathematics and Natural Sciences, Medan State University)
  • Received : 2023.03.12
  • Accepted : 2023.05.23
  • Published : 2023.06.22
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Abstract

Water scarcity decreases the rate of photosynthesis and, consequently, the yield of banana plants (Musa spp). In this study, transcriptome analysis was performed to identify photosynthesis-related genes in banana plants and determine their expression profiles under water stress conditions. Banana plantlets were in vitro cultured on Murashige and Skoog agar medium with and without 10% polyethylene glycol and marked as BP10 and BK. Chlorophyll contents in the plant shoots were determined spectrophotometrically. Two cDNA libraries generated from BK and BP10 plantlets, respectively, were used as the reference for transcriptome data. Gene ontology (GO) enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and visualized using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway prediction. Morphological observations indicated that water deficiency caused chlorosis and reduced the shoot chlorophyll content of banana plantlets. GO enrichment identified 52 photosynthesis-related genes that were affected by water stress. KEGG visualization revealed the pathways related to the 52 photosynthesisr-elated genes and their allocations in four GO terms. Four, 12, 15, and 21 genes were related to chlorophyll biosynthesis, the Calvin cycle, the photosynthetic electron transfer chain, and the light-harvesting complex, respectively. Differentially expressed gene (DEG) analysis using DESeq revealed that 45 genes were down-regulated, whereas seven genes were up-regulated. Four of the down-regulated genes were responsible for chlorophyll biosynthesis and appeared to cause the decrease in the banana leaf chlorophyll content. Among the annotated DEGs, MaPNDO, MaPSAL, and MaFEDA were selected and validated using quantitative real-time PCR.

Keywords

Acknowledgement

This research was supported by: the Master Degree to Doctoral Scholarship Program for Excellence Undergraduate (PMDSU), Directorate General of Higher Education, Ministry of National Education, Indonesia (2019-2020); and the Program of Research, Community Service, and Innovation (2020-2021), School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia.

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