• Title/Summary/Keyword: Chitosan Coating

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Coating using chitosan as a means to prevent dispersion of moist pellet feed in water of aquacultural farm, and bacterial pollution and its lipid oxidation during storage

  • Lee, Ki-Wan;Jeon, You-Jin;Heo, Moon-Soo;Song, Choon-Bok;Ha, Jin-Hwan;Kim, Se-Kwon
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.149-150
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    • 2001
  • A fish feed being used most widely in aquacultural farm is a moist pellet (MP) feed which has been being prepared by mixing of commercial dry feed and nutritional oil to raw fish ky. The two major problems in the use of MP feed is to be readily dispersed in water of an aquacultural farm, and to cause lipid oxidation and bacteria infection during storage. The former induce an environmental pollution of the farm and the latter lower nutritional values and develop fish diseasees. (omitted)

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Characteristics of Protein Chromatography by Affinity Membrane Mudule (친화성 막모듈에 의한 단백질 크로마토그래픽 특성)

  • 이광진;염경호
    • KSBB Journal
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    • v.13 no.2
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    • pp.125-132
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    • 1998
  • Protein affinity membrane was prepared via the coating of chitosan gel on the porous flat polysulfone membrane surface, followed by the immobilization f the reactive dye (Cibacron Blue 3GA) to the chitonsan gel. The maximum protein binding capacity of affinity membrane was about 70${\mu}g/cm^2$ determined by the batch adsorption experiments of human serum albumin (HSA). Using module of this membrane, the characteristics of protein chromatography were investigated through the experiments of elution and frontal chromatography of HSA. This membrane module promises as a chromatography column, since it represented a lower pressure drop and a greater reproducibility. The protein separation ratio was significantly influenced by the flow rate of mobile phase and the injection quantity of HSA. The dynamic protein binding capacity of module decreased from the equilibrium binding capacity with increasing flow rate and approached the value of 15 - 20 ${\mu}g/cm^2$ for flow rates above 6 mL/min.

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The effectiveness of controlled release device against E. faecalis : in vitro study

  • Hong, Sang-Hee;Yoo, Yoon-Jung;Lee, Seung-Jong;Kum, Kee-Yeon
    • Proceedings of the KACD Conference
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    • 2003.11a
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    • pp.560-560
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    • 2003
  • I. Objectives Intracanal medicament with antibacterial action is required to maximize the disinfection of the root canal system. The purpose of this study was to evaluate the efficacy of 2% chlorhexidine digluconate(CHX) and controlled release device(CRD) containing 20% CHX and chitosan coating, compared to calcium hydroxide as intracanal medicament against E. faecalis. II. Materials and Methods One hundred and twenty intact freshly extracted bovine incisors were used and were 0.5% NaOCl. Middle 1/3 portion of roots were sliced into 4mm thick section and cementum was removed using diamond burs and external diameter was approximally 6mm.(omitted)

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A Study on Affinity Chromatography of Protein by Flat and Hollow-Fiber Membrane Module (평판막 및 실관막 모듈에 의한 단백질의 친화성 크로마토그래피에 관한 연구)

  • 이광진;염경호
    • Membrane Journal
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    • v.8 no.1
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    • pp.50-58
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    • 1998
  • Protein affinity membranes were prepared via coating of chitosan gel on the porous flat and hollow-fiber polysulfone membranes, followed by the immobilization of the reactive dye (Cibacron Blue 3GA) to the chitosan gel. Maximum protein binding capacity of these affinity membranes was about 70 $\mu{g/cm}^2$. Using the affinity flat membrane module, the elution chromatography of human serum albumin (HSA) was performed to determine the optimum condition of eluent buffer. The optimum condition of eluent was the universal buffer solution of 0.06 M concentration containing 1 M KCl at pH 10. For the frontal chromatography of HSA using the flat module, the dynamic protein binding capacity was rapidly decreased from the equilibrium values with increasing flow rate and HSA concentration of the loading solution. However, in the case of hollow-fiber module, the dynamic binding capacity was maintained an equilibrium value without depending on the operating conditions. These results showed that the hollow-fiber module was more effective than the flat module as an affinity chromatography column.

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The Stabilization of Lactic Acid Bacteria, Bacillus polyfermenticus KJS-2 (유산간균인 Bacillus polyfermenticus KJS-2의 안정화)

  • Kim, Kang-Min;Lee, Jin-Young;Hong, Yong-Geun;Lee, Sang-Kil;Kang, Jae-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.8
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    • pp.1044-1048
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    • 2008
  • Bacillus polyfermenticus KJS-2 (Bp2) was isolated from $Bispan^{(R)}$, a commercially available probiotics consisting of more than 4 strains. The objective of this study was to investigate the effect of three-layer coating on the stabilty of Bp2. Bp2 was microencapsulated with sodium alginate using an air atomizer. The Bp2 loaded pellets were also coated with HFP-chitosan and HPMCP for oral delivery system. When compared to the uncoated Bp2, the survival of the three-layer coated Bp2 increased to approximately 63% (p<0.01) in a 30% ethanol solution, 54% (p<0.05) in an artificial gastric juice (pH 2), and 53% (p<0.05) in the bile acid (pH 5). When coated beads were stored at $100^{\circ}C$ and $130^{\circ}C$, Bp2 in coated beads was very stable (p<0.01) compared to uncoated Bp2.

Edible Coating Effects on Storage life of ‘Niikata’ Pear (신고배의 저장성에 미치는 가식성 코팅제의 효과)

  • 양용준
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.3 no.3
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    • pp.216-220
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    • 2002
  • In this study, edible coatings for ‘Niikata’ pears have been applied in order to provide an alternative way to control and extend market quality and shelf life during cold storage. Fruit treated with edible coatings had better effects on maintaining some quality features such as fresh weight, firmness and SSC content than non-coated fruits. However, non-coated fruits in terms of overall quality were better evaluated than fruit with edible film. It may be caused by various factors such as film thickness effect oil emulsion on fruit skin, unknown reactions between the fruit skin and functional groups in the chitosan structure and so on.

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Polysulfone/nanocomposites mixed matrix ultrafiltration membrane for the recovery of Maillard reaction products

  • Basu, Subhankar;Mukherjee, Sanghamitra;Balakrishnan, Malini;Deepthi, M.V.;Sailaja, R.R.N.
    • Membrane and Water Treatment
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    • v.9 no.2
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    • pp.105-113
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    • 2018
  • Maillard reaction products like melanoidins present in industrial fermentation wastewaters are complex compounds with various functional properties. In this work, novel ultrafiltration (UF) mixed matrix membrane (MMM) composed of polysulfone (PSF) and nanocomposites was prepared through a phase inversion process for the recovery of melanoidins. Nanocomposites were prepared with acid functionalized multiwalled carbon nanotubes (MWCNTs) as the reinforcing filler for chitosan-thermoplastic starch blend. Higher nanocomposites content in the PSF matrix reduced the membrane permeability and melanoidins retention indicating tighter membrane with surface defects. The membrane surface defects could be sealed with dilute polyvinyl alcohol (PVA) solution. The best performing membrane (1% nanocomposites in 18% PSF membrane sealed with 0.25% PVA coating) resulted in uniform melanoidins retention of 98% and permeability of 3.6 L/m2 h bar over a period of 8h. This demonstrates a low fouling PSF membrane for high melanoidins recovery.

Thermal resistance effect of graphene doped zinc oxide nanocomposite in fire retardant epoxy coatings

  • Rao, Tentu Nageswara;Hussain, Imad;Riyazuddin, Riyazuddin;Koo, Bon Heun
    • Journal of Ceramic Processing Research
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    • v.20 no.4
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    • pp.411-417
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    • 2019
  • Graphene doped zinc oxide nanoparticles (G-ZnO) were prepared using modified hummer's technique together with the ultrasonic method and characterized by field emission scanning electron microscopy (FESEM), X-ray powder diffraction (XRD), fourier-transform infrared spectroscopy (FTIR) and high-resolution transmission electron microscopy (HRTEM). Different samples of epoxy resin nanocomposites reinforced with G-ZnO nanoparticles were prepared and were marked as F1 (without adding nanoparticles), F2 (1% w/w G-ZnO), and F3 (2% w/w G-ZnO) in combination of ≈ 56:18:18:8w/w% with epoxy resin/hardener, ammonium polyphosphate, boric acid, and Chitosan. The peak heat release rate (PHRR) of the epoxy nanocomposites was observed to decrease dramatically with the increasing G-ZnO nanoparticles. However, the LOI values increased significantly with the increase in wt % of G-ZnO nanoparticles. From the UL-94V data, it was confirmed that the F2 and F3 samples passed the flame test and were rated as V-0. The results obtained in the present work clearly revealed that the synthesized samples can be used as efficient materials in fire-retardant coating technology.

Antibacterial effects of Chitosanon-ascorbate Treated Kwamaegi Prepared on Microorganism Contamination (Chitosan-ascorbate 처리 과메기에 있어서 오염미생물에 대한 저해효과)

  • Kim, Young-Sook;oh, Seung-Hee;Kim, Soon-Dong
    • KSBB Journal
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    • v.24 no.2
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    • pp.156-162
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    • 2009
  • We examined saury, herring, gizzard shad kwamaegi to measure of microbic contamination rate of kwamegi that are sold in the market now. In the total bacteria, staphylococcus, peroxide value, and microorganisms is inhibited that from sample that we treated a substance with chitosan-ascorbate (CA) and other orders deep water (DW), ginseng steamed red and wine (GRW), NT (not treated). When we compared between SGRW and SNT, SCA show us more inhibition effect 0.22-0.49 log cycle in the total aerobacter. When we compared between HDW and HNT, HCA restraint 0.05-0.45log cycle, and when we compared between GDW and GNT, GCA inhibited 0.45 log cycle. In the coliform and E. coli, growths of microorganisms were inhibited followed order by treatment of CA, NT, and DW. GDW, HCA and HNT checked enough amount of water from the moisture measurement; but SGW, GCA, HEW and SCA showed 7-15% lack of moisture, and SNT and GNT have 10% more moisture. Peroxide value is changed to 41-51meq/kg when we did treat CA in there and a side that didn't add antimicrobial expressed the result numerically that 56-58meq/kg. In the sensory evaluation, customer gave preference to followed by Saury kwamaegi, herring, and gizzard shad kwamaegi. We have a point of view when kwamaeki manufactured if we add natural antibiotic and it uses to vacuum drying, we would inhibited of multiplication of microorganism, and of peroxides.

Recent research trends of post-harvest technology for king oyster mushroom (Pleurotus eryngii) (큰느타리버섯 수확후 관리기술 최근 연구 동향)

  • Choi, Ji-Weon;Yoon, YoeJin;Lee, Ji-Hyun;Kim, Chang-Kug;Hong, Yoon-Pyo;Shin, Il Sheob
    • Journal of Mushroom
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    • v.16 no.3
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    • pp.131-139
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    • 2018
  • The king oyster mushroom (Pleurotus eryngii) is widely consumed because of its flavor, texture, and its functional properties such as antioxidant activity and prebiotic effects. However, long-term product storage and transportation (e.g., export) are difficult because of its limited durability. The shelf-life of king oyster mushroom is affected by environmental factors such as temperature, humidity, gas composition, and ventilation, which may affect sensory characteristics including respiration rate, texture, moisture, flavor, color, and pH. The major problems regarding storage of mushrooms are browning, flavor changes, and softening. To address these problems, novel preservation techniques were developed, and more durable variants were bred. Different drying methods, gamma irradiation, chitosan coating, modified atmosphere (MA) packaging, and controlled atmosphere (CA) storage were evaluated in order to extend the shelf-life of king oyster mushrooms. Freeze drying showed better results for the preservation of mushrooms than other drying methods. Irradiation with 1 kGy was more effective for extending mushroom shelf-life than higher doses. The preservative performance of chitosan-based films was improved by combining the compound with other hydrocolloids, such as oil, protocatechuic acid, and wax. The CA storage conditions recommended for king oyster mushrooms are 5kPa $O_2$ and 10 to 15kPa $CO_2$ at temperatures below $10^{\circ}C$. Active MA packaging with microperforated PP film was also effective for maintaining quality during storage.