• Journal of Life Science
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• v.19 no.3
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• pp.390-396
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• 2009

In this study, extracts from S. officinalis were tested for antioxidative effects and inhibitory activities against $\alpha$-amylase, angiotensin converting enzyme (ACE) and xanthine oxidase (XOase). The content of total phenolic compounds in water, 60% ethanol, 60% methanol and 60% acetone extracts were 36.2, 42.7, 40.3 and 39.6 mg/g, respectively. In 60% ethanol extracts, the EDA by DPPH free radical scavenging test of S. officinalis was $66.3{\pm}2.2%$ at $200{\mu}g/ml$. The inhibition rate of ABTS was $97.6{\pm}0.1%$, the antioxidant protection factor was $2.26{\pm}0.63$ PF, and TBARS was $0.62{\pm}0.05$ (${\times}100{\mu}M$ in the control and $0.29{\pm}0.02$ (${\times}100{\mu}M$). Also in 60% ethanol extracts of S. officinalis, the inhibitory activity against XOase was 78% and was not shown to be against ACE. According to the $12.6{\pm}0.14\;mm$ of clear zone formed, the inhibition rate against $\alpha$-Amylase was 7.6% at $200{\mu}g/ml$ of phenolics content. Antimicrobial activities of 60% ethanol extracts of S. officinalis against Helicobacter pylori exhibited an inhibition rate of $12.5{\sim}66.1%$ according to the $10{\sim}15\;mm$ of clear zone at $50{\sim}200{\mu}g/ml$. The results suggest that the 60% ethanol extracts from Salvia officinalis L. will be useful as natural antioxidants and functional food sources.

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.295-301
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• 2006

This study has been designed to evaluate the efficacy of MD in-ovo vaccination in broiler and to find the contamination level of MD virus in broiler farms. A nested PCR test was used to find the level of filed contamination of pathogenic MD virus in the commercial broiler farms. The positive rate of farms contaminated with pathogenic MD virus was 26.09%. And to evaluate the efficacy of MD vaccination, performance factors such as the mortality, feed conversion rate and body weight at market age has been recorded and compared among farms. There was no difference on these factors between vaccinated and non-vaccinated farms except performance. We need further investigation of the film with low performance index. The B/B ratio, which is common index for the evaluation of bursal changes, was calculated and compared between vaccinated and non-vaccinated group There were significant decreases of B/B ratio in the both group without any statistical difference between groups. The reason of decreased bursal size may be due to other infection such as IBD. From this study, we can know that the contamination rate of fm with pathogenic MD virus was very low and not different from previous study. However, the difference of the contamination rate in different locations recommends periodical monitoring on these areas.

• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.103-108
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• 2020

Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacteria and can cause severe foodborne disease. For STEC detection, conventional culture methods have disadvantages in the fact that conventional culture takes a long time to detect and PCR can also detect dead bacteria. To overcome these problems, we suggest a bacteriophage amplification assay, which utilizes the ability of bacteriophages to infect living cells and their high specificity. We used a combination of six bacteriophages infecting E. coli to make the bacteriophage cocktail and added ferrous ammonium sulfate as a virucidal agent to remove free-bacteriophages. When cherry tomato and paprika were artificially inoculated with the cocktail at a final concentration of around 3 log CFU/mL and were enriched for at least 5 h in mTSB broth with Novobiocin, approximately 2-3 log PFU/mL were detected through the bacteriophage amplification assay. Therefore, bacteriophage amplification assay might be convenient and a useful method to detect STEC in a short period of time.

• Korean Journal of Environmental Agriculture
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• v.33 no.2
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• pp.111-120
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• 2014

BACKGROUND: This study was conducted to investigate the levels of pesticide residues in fruits and to assess their risk to human health. METHODS AND RESULTS: Monitoring of 215 samples of fruits collected from local markets in incheon during 2013 was performed. 259 pesticides were analyzed by multi-residue method and Quick, Easy, Cheap, Effective, Rugged, and safe/Mass/Mass(QuEChERS/MS/MS) method using Gas Chromatography-Electron Capture Detector/Nitrogen Phosphorus Detector(GC-ECD/NPD), GC-MS, LC(Liquid Chromatography-Mass/Mass(LC-MS/MS) and High Performance Liquid Chromatography-Photodiode Array/Fluorescence Detector(HPLC-PDA/FLD). In 56.3% of the samples detected pesticide residues and were not found to exceed Maximum Residue Limits(MRL). The highest detected samples were found in citrus fruits(83.9%). Among the detected compounds, carbendazim(13.1%), imazalil (11.7%), thiabendazole(10.7%) and fludioxonil(9.8%) were frequently found in fruits. A risk assessment of pesticide residues in fruits was performed by calculating Estimated Daily Intake(EDI) and Acceptable Daily Intake(ADI). Also, we were evaluated removal efficiency of pesticide residues by washing and peeling. The removal efficiency of pesticide residues in citrus and tropical fruits by peeling processes were 91.6%. After the washing process, the removal rates were 43.1%(Cherry, Grape, Blueberry). CONCLUSION: The level of pesticide residues in fruits was within the MRL. The range of %ADI values was from 0.00011 to 0.98795%. The process of washing or peeling reduces the level of pesticide residues. The results of this research concluded that the detected pesticides are not harmful to human being.

• Korean Journal of Organic Agriculture
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• v.25 no.4
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• pp.737-747
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• 2017

This experiment was carried out to determine the optimal concentration of agricultural organic materials using sulfur and oil for the insect pest control in pepper and cherry tomato cultivation. The control value of aphids and Oriental tobacco budworm (OTB) was examined one day after spraying with sulfur preparation (SP) (0.33~0.17%), oil preparations (OP) (2.00~0.33%), SP+OP, OP+ginkgo leaf extracts (GLE), SP+OP+GLE on the "Super Manidaa"pepper. The aphid control in pepper was complete by applications of SP+OP (0.25+1.00%) in the early growth stage and the control value was above 98.1% by the application of OP+GLE (1.00+1.00 %), SP+OP+GLE (0.25+1.0+1%), SP+OP+GLE (0.25+1.0+0.5%) in the middle to late growth stage while showing 0% in the control treatment. The OTB was completely controlled by the 3 times application with the high concentration of SP+OP (0.25+1.00%) in pepper cultivation. This result indicates that the oil and the sulfur preparations should be applied at low concentration before insect pests do not appeared, and then sprayed at the high concentration after they appear at pepper plant. The greenhouse whitefly in 'Minichal' tomatoes was completely controlled by three times application of SP (0.25~0.33), OP (1.0~2.00%). and all the treatment of SP+OP. However, continuous control with intervals of 1~3 days was considered favorable in the tomato plant. By the periodical control with agricultural organic materials using sulfur and oil, the greenhouse whitefly, which is a high-temperature insect pest, several moths of OTB did not occur at all. In conclusion, SP+OP (0.17%+0.33%) treatment was the most economical combination to control the aphid, OTB, and greenhouse whitefly in pepper and tomato cultivation when considering operating cost. In addition, we recommend that SP should not be sprayed on the plant shoots during the day time from July to August because of high temperature.

• Research in Plant Disease
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• v.15 no.3
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• pp.198-201
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• 2009

Bacterial wilts, caused by Ralstonia solanacearum, is a very destructive disease to tomato plants (Solanum lycopersicum) in Korea. Selection of resistant cultivar is the best way to prevent or reduce the occurrence of this disease. Thirty-nine tomato cultivars, twenty-one cherry tomato cultivars and thirteen rootstock tomato cultivars were inoculated with Ralstonia solanacearum, to evaluate tomato cultivars for resistance against bacterial wilts. Thirty-seven cultivars were highly susceptible to bacterial wilts and 61-100% of their whole tissue became wilted within 10 days after inoculation. Twenty-four cultivars showed moderate resistance and twelve were resistant to bacterial wilts. In an evaluation of 73 major commercial cultivars, 'Lilyance', 'TP-7', 'Choice', 'Dadaki', 'Akiko', 'Redstar', 'Match', 'B-blocking', 'Magnet', 'Support', 'Friend' and 'Special' were found to have a high level of resistance to bacterial wilts of tomatoes.

• Research in Plant Disease
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• v.8 no.2
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• pp.101-106
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• 2002

For the detection of Tobacco masaic virus (TMV), Tomato mosaic virus (ToMV), and Cucumber mosaic virus (CMV), tomato seeds of 11 table tomato and 7 cherry tomato cultivars were assayed by DAS-ELISA. Among the cultivars, TMV and ToMV were detected from 9 cultivars at the rates lower than 20% and 16%, respectively. In the assay on seed transmission rates, ToMV and CMV were detected as high as 24% and 8% , respectively, but TMV was not detected. In field survey on these viruses from tomato plants of 10 different places in Chungbuk province, ToMV and CMV were detected from most fields. TMV was detected from only 3 fields. The highest detection rates of these viruses were recorded in Cheongwon for TMV Chungju for ToMV, and the other locality of Chungju far CMV. It was difficult to find any relationship between the growth stage of tomato and infection rates. TMV usually caused mosaic on leaves while ToMV caused various symptoms including yellows, necrosis, and mottling. CMV-infected tomato plants showed symptoms of shoestring, fern leaf, and yellows.

• Research in Plant Disease
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• v.11 no.2
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• pp.213-216
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• 2005

Tomato spotted wilt virus (TSWV) occurred abruptly with a high incidence rate in 14 vegetable crops in Anyang area, Gyunggido in 2004. TSWV was identified by the symptoms on the indicator plants, immunocaptured reverse transcription-polymerase chain reaction (IC/RT-PCR), virion captured (VC)RT-PCR and RT-PCR using total RNA from the infected plants. Vegetable crops infected with TSWV included table tomato, cherry tomato, red pepper, lettuce, chicory, red leaf chicory, red mustard, dragon tongue, treviso, potato, perilla, sesame, pumpkin, and ssamchoo (hybrid of oriental cabbage and cabbage). The incidence of TSWV in fields ranged from 30 to $100\%$. TSWV usually produced necrosis, wilt and/or severe mosaic with typical single or double ring spots on the leaves. TSWV could be detected in Frankliniella occidentalis collected from the crops in the fields with $90\%$ rate by IC/RT-PCR.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.47-56
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• 2014

Purpose: In protected crop production facilities such as greenhouse and plant factory, farmers should be present and/or visit frequently to the production site for maintaining optimum environmental conditions and better production, which is time and labor consuming. Monitoring of environmental condition is highly important for optimum control of the conditions, and the condition is not uniform within the facility. Objectives of the paper were to investigate spatial and vertical variability in ambient environmental variables and to provide useful information for sensing and control of the environments. Methods: Experiments were conducted in a strawberry-growing greenhouse (greenhouse 1) and a cherry tomato-growing greenhouse (greenhouse 2). Selected ambient environmental variables for experiment in greenhouse 1 were air temperature and humidity, and in greenhouse 2, they were air temperature, humidity, PPFD (Photosynthetic Photon Flux Density), and $CO_2$ concentration. Results: Considerable spatial, vertical, and temporal variability of the ambient environments were observed. In greenhouse 1, overall temperature increased from 12:00 to 14:00 and increased after that, while RH increased continuously during the experiments. Differences between the maximum and minimum temperature and RH values were greater when one of the side windows were open than those when both of the windows were closed. The location and height of the maximum and minimum measurements were also different. In greenhouse 2, differences between the maximum and minimum air temperatures at noon and sunset were greater when both windows were open. The maximum PPFD were observed at a 3-m height, close to the lighting source, and $CO_2$ concentration in the crop growing regions. Conclusions: In this study, spatial, vertical, and temporal variability of ambient crop growing conditions in greenhouses was evaluated. And also the variability was affected by operation conditions such as window opening and heating. Results of the study would provide information for optimum monitoring and control of ambient greenhouse environments.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.599-604
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• 2015

This study was conducted the vegetation clustering analysis for the 3 mountains of Mt. Bulam, Mt. Daemo, Mt. Bonghwa which were the urban forests of Seoul. Based on the results of the analysis related to the vegetation clustering, it was found that the clustering of Mt. Bulam consisted of pine trees(Pinus densiflora)-Mongolian oak(Quercus mongolica), Hornb eam(Carpinus laxiflora)-Pitch pine(P. rigida), oriental oak(Q. variabilis) - a wild pear tree(Sorbus alnifolia) while the clustering of Mt. Daemo consisted of Pitch pine-Japanese larch(Larix leptolepis), Poplar(Populus tomentig landulosa)- black birch(Betula davurica pall). Meanwhile, the clustering of Mt. Bonghwa consisted of pine trees-a wild pear tree Community and Sawtooth oak(Q. acutissima)-Cherry Blossoms(Prunus serrulata). In relation to the similarity index by region in Mt. Bulam, Mt. Daemo, and Mt. Bonghwa, the similarity index of Mt. Bulam and Mt. Daemo stood at as high as 0.634, suggesting the distribution of similar vegetation, and the dominance index of the Mt. Daemo region was found to be 0.166 which suggests the dominance of many species compared to other regions. In addition, the results of species diversity showed that Mt. Daemo had the highest stability, and the species diversity, maximum species diversity, evenness indices were highest in Mt. Bulam, followed by Mt. Bonghwa and Mt. Daemo. The dominance index was the lowest in Mt. Bulam, followed by Mt. Bonghwa and Mt. Daemo.