• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.505-509
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• 2002

In the course of screening program for VHR DS-PTPase (dual-specificity protein tyrosine phosphatase) from natural sources, Gastrodia elata was selected. One compound showing potent inhibitory activity was isolated by the solvent extraction and column chromatography including silica gel, ODS RP-18, Sephades LH-20, and HPLC. This compound was identified as baicalein by several NMR techniques such as $^1H-NMR$, $^{13}C-NMR$, and DEPT. Baicalein showed selective inhibitory activity against VHR DS-PTPase with $IC_{50}=2.4\;{\mu}M$, and showed cytotoxicity against several human cancer cell lines with an $GI_{50}$ of $5.26{\sim}12.93\;{\mu}g/mL$ range, including, melanoma (LOX-IMVI), lung cancer (NCI H23 and A549), colon cancer (HCT 116 and SW 620), prostate cancer (PC-3), and leukemia (MOLT 4F).

• Journal of Korean Neurosurgical Society
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• v.67 no.2
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• pp.146-157
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• 2024

Objective : Chronic subdural hematomas (cSDHs) are generally known to result from traumatic tears of bridging veins. However, the causes of repeat spontaneous cSDHs are still unclear. We investigated the changes in vasculature in the human dura mater and outer membrane (OM) of cSDHs to elucidate the cause of their spontaneous repetition. Methods : The dura mater was obtained from a normal control participant and a patient with repeat spontaneous cSDHs. The pathological samples from the patient included the dura mater and OM tightly adhered to the inner dura. The samples were analyzed with a particular focus on blood and lymphatic vessels by immunohistochemistry, 3-dimensional imaging using a transparent tissue clearing technique, and electron microscopy. Results : The dural border cell (DBC) layer of the dura mater and OM were histologically indistinguishable. There were 5.9 times more blood vessels per unit volume of tissue in the DBC layer and OM in the patient than in the normal control. The DBC layer and OM contained pathological sinusoidal capillaries not observed in the normal tissue; these capillaries were connected to the middle meningeal arteries via penetrating arteries. In addition, marked lymphangiogenesis in the periosteal and meningeal layers was observed in the patient with cSDHs. Conclusion : Neovascularization in the OM seemed to originate from the DBC layer; this is a potential cause of repeat spontaneous cSDHs. Embolization of the meningeal arteries to interrupt the blood supply to pathological capillaries via penetrating arteries may be an effective treatment option.

• Tuberculosis and Respiratory Diseases
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• v.67 no.4
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• pp.325-330
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• 2009

Background: Pulmonary tuberculosis (TB) is still common disease among the elderly patients in Korea where the overall incidence of TB is decreasing. Adverse drug reactions (ADR) associated with anti-TB drugs occurs frequently. Especially the aged tends to have more frequent ADRs than younger ones. These ADRs can cause significant morbidity, compromise therapeutic effects of drugs and even induce drug resistance. Therefore we evaluated the effect of ADRs on the first-line anti-TB drugs in elderly patients with active pulmonary TB. Methods: We retrospectively reviewed the charts and radiological findings of the patients with 65 and older who were bacteriologically confirmed as active TB and treated with standard anti-TB drugs for at least 6 months. Major ADR was defined with temporary or continuous stop of any first-line drugs intake. Results: An ADR was noted in 54% of all patients. The incidence of major ADR was 32% in all elderly patients. Dermatologic ADR (9%) was the most common among the major ADRs. GI trouble (8%), arthralgia (6%), visual change (6%), hepatotoxicity (4%), and fever (1%) were also noted. The drugs responsible for major ADR were ethambutol (62%), pyrazinamide (35%), rifampin (18%) and isoniazid (9%). Major ADRs were associated with higher ESR level at the initiation of anti-TB drugs. Conclusion: First-line anti-TB drugs in elderly patients frequently caused the major ADRs. Therefore the elderly patients receiving anti-TB drugs should be closely monitored and better tolerable therapy should be considered as part of a TB research agenda.

• Journal of the Korea Concrete Institute
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• v.18 no.2 s.92
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• pp.249-255
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• 2006

Recently, interest grew on the quality of aggregates following the diminution of primary resources from river as to grow construction demand and the low grade of nature sand like sea sand. following, need is to diversify the supply sources of fine aggregates which are excessively relying on sea sand and urgency is to find as soon as possible aggregate resources that can substitute sea sand. On the other hand, various fine aggregates we utilized to produce concrete in the domestic construction fields. However, few studies have been systematically investigated on the effects of such fine aggregates on concrete properties. Therefore, this study examined the effects of comparatively widely used fine aggregates in the domestic construction fields on the shrinkage, durability and watertightness of concrete. Results revealed that drying shrinkage increases, and durability and watertightness degrades for concrete using crushed sand than natural fine aggregates like sea sand and river sand. Especially, the use of crushed sand exhibiting bad grain shape and grade was larger adverse effect on the quality of concrete. In addition, appropriate adjustment of the grain shape and grade during the blending of crushed sand exhibiting bad grain shape and grade with natural aggregates appeared to enhance the shrinkage and durability of concrete.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.359-364
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• 2012

We conducted this study to identify the optimal milling degree for improving sensory quality of cooked rice and minimizing yield loss. The samples were analyzed for their physicochemical properties and sensory qualities using five different degree of milling(5.5~13.5%, based on brown rice). With increasing of milling degree, amylose content, breakdown, adhesiveness of cooked rice increased, whereas protein content, Mg/K equivalence ratio, fat content, setback and hardness of cooked rice decreased. In sensory quality test, the preference was significantly higher in the 9.5% and 11.5% milling degree. However, considering the yield of milled rice, the optimal milling degree for improving sensory quality of cooked rice was 9.5%.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.57-66
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• 2003

Single and 28-day repeated dose toxicity studies of botulimnn toxin type A were carried out in ICR mice and Sprague-Dawley rats, respectively. In the single dose toxicity study, botulinwn toxin was injected intraperitoneally to male and female mice at a single dose of 40, 59, 89 133 and 200 ng/10 ml saline/kg. All animals died from 59 ng/kg group. Some clinical signs, such as decrease in locomotor activity, dyspnea, prone position and ptosis, were observed in most of both sexes from 59 ng/kg group, but no signs were seen in all animals at 40 ng/kg group. The results showed that the median lethal dose of botulinum toxin might be in the range of 40-59 ng/kg in both sexes. In the repeated dose toxicity study, the test material was administered intradermally for 28 days at doses of 0 (vehicle-treated control), 1.25, 2.5, 5.0 and $10.0ng/head/50{\mu}{\ell}$ saline in male and female rats. No test material-related changes were noted in survivals, clinical signs, food and water consumptions and gross finding in any group. Botulinum toxin treatment significantly decreased the body weight gain rate in male of 5.0 ng/head group and over and in female of 10.0 ng/head group compared to vehicle-treated control. One or more relative organ weights (i.e., spleen, thymus, liver and kidney) were increased significantly from 5.0 ng/head group compared to vehicle-treated control in both sexes. Serum biochemistry revealed increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase, total protein and albumin in male, and increases in AST and ALT and decreases in $K^+$ and $Cl^-$ in female without dose-pendent manners. In the histopathological study, physical stimulation by needle caused slight inflammations of dennis. In addition, botulinum toxin treatment induced denervation of nerve cell and disuse of muscle, resulting in atrophy of skeletal muscle in both sexes from 2.5 ng/head group. When the antibodies to toxin were determined in all animals, a significant increase in serum antibodies was observed from 5.0 ng/head group. The results showed that the NOAEL of botulinum toxin might be 1.25 ng/head for 28-day repeated dose toxicity in rats.

• Clean Technology
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• v.23 no.3
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• pp.302-307
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• 2017

This study was conducted to evaluate the feasibility of in situ soil flushing for TPH-contaminated soil remediation with column test. The soil texture of the soil was sand and the initial TPH concentration was $9,369mg\; kg^{-1}$. 0.1% Tween-80 was selected as surfactant solution. And the acrylic and the glass syringe columns were used as reactors. In the acrylic column test, 35% of the initial TPH was removed in 1 PV of flushing and approximately 40% in 5 PV and finally 7 PV showed about 60%. The glass column test showed 3 ~ 12% higher removal efficiency than that of acrylic test until 5 PV of flushing. However, there was no difference in TPH removal efficiency when 7 PV of surfactant was finally flushed. Both of alum only and alum+polymer mixed surfactants showed also the best coagulation efficiency in $150mg\;L^{-1}$ of concentraion. When Tween 80 was newly dissolved in 0.1% to the recovered solution after the coagulation treatment, the removal efficiency was increased from 32.0% to 41.0% in comparison to the new 0.1% Tween 80 solution without reuse by coagulation treatment.

• Journal of the Korean Applied Science and Technology
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• v.32 no.4
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• pp.740-747
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• 2015

This research was performed to evaluate the feasibility of in situ soil flushing for TPH-contaminated soil remediation. It was conducted in batch test as fundamental research for in situ soil flushing. The 30% of initial TPH concentration was removed by shaking only in batch test. The removal efficiency of TPH in case of groundwater as surfactant dilution solution was approximate 2~6% lower than that of distilled water. Mixing ratio of soil to surfactant solution did not practically effect on the TPH removal efficiency. In the experiment of using single or mixed surfactant solution with 0.1~4.0 wt%, Tween-80, SWA-1503, SWA-1503+SDS showed averagely over 80%. It was determined that the optimum surfactant concentration was 0.1 wt% because there was no significant difference between concentrations of 0.1~4.0 wt%.

• Journal of the Korea Concrete Institute
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• v.18 no.4 s.94
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• pp.459-467
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• 2006

Recently, interest grew on the quality of aggregates following the diminution of primary resources from river as to grow construction demand and the low grade of nature sand like sea sand. Following, need is to diversify the supply sources of fine aggregates which are excessively relying on sea sand and urgency is to find as soon as possible aggregate resources that can substitute sea sand. On the other hand, various fine aggregates are utilized to produce concrete in the domestic construction fields. However, few studies have been systematically investigated on the effects of such fine aggregates on concrete properties. Therefore, this study examined the effects of comparatively widely used fine aggregates in the domestic construction fields on the quality of concrete through the analysis of the effects of such fine aggregates on the physical properties of fresh concrete and strength of hardened concrete. Results revealed that crushed sand degraded the fluidity and air entraining of concrete compared to natural aggregates like sea sand and river sand. Especially, the use of crushed sand exhibiting bad grain shape and grade was larger adverse effect on the physical properties of concrete. The type of fine aggregates appeared to have negligible influence on the strength for W/C of 55%, 45% while crushed sand decreased the strength for W/C of 35% compared to natural aggregates. It analyzed that the combination of crushed sand exhibiting bad grain shape and grade with natural aggregates improved the characteristics of fresh concrete and had negligible influence on the strength.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.2
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• pp.93-100
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• 2012

We examined the contents of amino acids to provide the basic data for the utilization of white panicled rice (Oryza sativa L., WPR) cultured in reclaimed paddy field of Bigumdo of Korea in 2011. WPR used in this study were collected in 1000 ha of reclaimed paddy field of Bigumdo. Crude protein contents of whole rice have the highest in leaf blade, followed by whole grain, leaf sheath and stem. The contents of total amino acids showed the highest trend in region received the most severe damage, but the lowest trend in region of normal growth. The content of glutamic acid was the highest among amino acids in leaf blade, whole grain, leaf sheath and stem collected in all regions, but the content of methionine was the lowest. Therefore, the contents of amino acids showed a similar level between the region occurred WPR and the region of normal growth. Further research on the nutritional aspects of forage must be performed because nutritive values of WPR were influenced by the degree of damage of WPR.