• Title/Summary/Keyword: Chen Yan

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RAPD Fingerprinting for the Species Identification of Animals

  • Huang, Mu-Chiou;Horng, Yan-Ming;Huang, Hsiu-Lin;Sin, Yen-Long;Chen, Ming-Jaw
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1406-1410
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    • 2003
  • The studies were based on the RAPD fingerprinting for the species identification of animals. The genomic DNA samples of ostriches, Taiwan local chickens, Aboracres broilers, Leghorn chickens, quails, doves, emus, Beltville small white turkeys, pheasants, Chinese geese, mule ducks, Holstein cattle and Landrace pigs were amplified with random primers by RAPD-PCR for fingerprinting. The results showed that the varied band patterns of DNA fingerprints were generated from templates depending on the kinds of primers or animal species. The same primer applied to the same breed, all of the main bands are similar, but which were different among species. In order to try to identify the species from the mixture of meat by RAPD fingerprinting, the meat of ostrich and cattle was mixed in different ratios for this study. The results showed that it could be easily and precisely distinguished according to the band distribution of RAPD patterns.

A Low-power High-resolution Band-pass Sigma-delta ADC for Accelerometer Applications

  • Cao, Tianlin;Han, Yan;Zhang, Shifeng;Cheung, Ray C.C.;Chen, Yaya
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.17 no.3
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    • pp.438-445
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    • 2017
  • This paper presents a low-power high-resolution band-pass ${\Sigma}{\Delta}$ ADC for accelerometer applications. The proposed band-pass ${\Sigma}{\Delta}$ ADC consists of a high-performance 6-th order feed-forward ${\Sigma}{\Delta}$ modulator with 1-bit quantization and a low-power, area-efficient digital filter. The ADC is fabricated in 180 nm 1P6M mixed-signal CMOS process with a die area of $5mm^2$. This high-resolution ADC got 90 dB peak signal to noise plus distortion ratio (SNDR) and 96 dB dynamic range (DR) over 4 kHz bandwidth, while the intermediate frequency (IF) is shifting from 100 KHz to 200 KHz. The power dissipation of the chip is 5.6 mW under 1.8 V (digital)/3.3 V (analog) power supply.

A Novel Monoclonal Antibody Induces Cancer Cell Apoptosis and Enhances the Activity of Chemotherapeutic Drugs

  • Xu, Heng;Tian, Yan-Na;Dun, Bo-Ying;Liu, Hai-Tao;Dong, Guang-Kuo;Wang, Jin-Hua;Lu, Shang-Su;Chen, Bo;She, Jin-Xiong
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.11
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    • pp.4423-4428
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    • 2014
  • A novel monoclonal antibody (mAb), known as AC10364, was identified from an antibody library generated by immunization of mice with human carcinoma cells. The mAb recognized proteins in lysates from multiple carcinoma cell lines. Cell cytotoxicity assays showed that AC10364 significantly inhibited cell growth and induced apoptosis in multiple carcinoma cell lines, including Bel/fu, KATO-III and A2780. Compared with mAb AC10364 or chemotherapeutic drugs alone, the combination of mAb AC10364 with chemotherapeutic drugs demonstrated enhanced growth inhibitory effects on carcinoma cells. These results suggest that mAb AC10364 is a promising candidate for cancer therapy.

Cellular Traffic Offloading through Opportunistic Communications Based on Human Mobility

  • Li, Zhigang;Shi, Yan;Chen, Shanzhi;Zhao, Jingwen
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.3
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    • pp.872-885
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    • 2015
  • The rapid increase of smart mobile devices and mobile applications has led to explosive growth of data traffic in cellular network. Offloading data traffic becomes one of the most urgent technical problems. Recent work has proposed to exploit opportunistic communications to offload cellular traffic for mobile data dissemination services, especially for accepting large delayed data. The basic idea is to deliver the data to only part of subscribers (called target-nodes) via the cellular network, and allow target-nodes to disseminate the data through opportunistic communications. Human mobility shows temporal and spatial characteristics and predictability, which can be used as effective guidance efficient opportunistic communication. Therefore, based on the regularity of human mobility we propose NodeRank algorithm which uses the encounter characteristics between nodes to choose target nodes. Different from the existing work which only using encounter frequency, NodeRank algorithm combined the contact time and inter-contact time meanwhile to ensure integrity and availability of message delivery. The simulation results based on real-world mobility traces show the performance advantages of NodeRank in offloading efficiency and network redundant copies.

A new equivalent friction element for analysis of cable supported structures

  • Yan, Renzhang;Chen, Zhihua;Wang, Xiaodun;Liu, Hongbo;Xiao, Xiao
    • Steel and Composite Structures
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    • v.18 no.4
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    • pp.947-970
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    • 2015
  • An equivalent friction element is proposed to simulate the friction in cable-strut joints. Equivalent stiffness matrixes and load vectors of the friction element are derived and are unified into patterns for FEM by defining a virtual node specially to store internal forces. Three approaches are described to verify the rationality of the new equivalent friction element: applying the new element in a cable-roller model, and numerical solutions match well with experimental results; applying the element in a continuous sliding cable model, and theoretical values, numerical and experimental results are compared; and the last is applying it in truss string structures, whose results indicate that there would be a great error if the cable of cable supported structures is simulated with discontinuous cable model which is usually adopted in traditional finite element analysis, and that the prestress loss resulted from the friction in cable-strut joints would have adverse effect on the mechanical performance of cable supported structures.

Elimination of environmental temperature effect from the variation of stay cable force based on simple temperature measurements

  • Chen, Chien-Chou;Wu, Wen-Hwa;Liu, Chun-Yan;Lai, Gwolong
    • Smart Structures and Systems
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    • v.19 no.2
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    • pp.137-149
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    • 2017
  • Under the interference of the temperature effect, the alternation of cable force due to damages of a cable-stayed bridge could be difficult to distinguish. Considering the convenience and applicability in engineering practice, simple air or cable temperature measurements are adopted in the current study for the exclusion of temperature effect from the variation of cable force. Using the data collected from Ai-Lan Bridge located in central Taiwan, this work applies the ensemble empirical mode decomposition to process the time histories of cable force, air temperature, and cable temperature. It is evidently observed that the cable force and both types of temperature can all be categorized as the daily variation, long-term variation, and high-frequency noise in the order of decreasing weight. Moreover, the correlation analysis conducted for the decomposed variations of all these three quantities undoubtedly indicates that the daily and long-term variations with different time shifts have to be distinguished for accurately evaluating the temperature effect on the variation of cable force. Finally, consistent results in reducing the range of cable force variation after the elimination of temperature effect confirm the validity and stability of the developed method.

Nonlinear vibration analysis of an embedded multi-walled carbon nanotube

  • Wu, Chih-Ping;Chen, Yan-Hong;Hong, Zong-Li;Lin, Chia-Hao
    • Advances in nano research
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    • v.6 no.2
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    • pp.163-182
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    • 2018
  • Based on the Reissner mixed variational theorem (RMVT), the authors present a nonlocal Timoshenko beam theory (TBT) for the nonlinear free vibration analysis of multi-walled carbon nanotubes (MWCNT) embedded in an elastic medium. In this formulation, four different edge conditions of the embedded MWCNT are considered, two different models with regard to the van der Waals interaction between each pair of walls constituting the MWCNT are considered, and the interaction between the MWCNT and its surrounding medium is simulated using the Pasternak-type foundation. The motion equations of an individual wall and the associated boundary conditions are derived using Hamilton's principle, in which the von $K{\acute{a}}rm{\acute{a}}n$ geometrical nonlinearity is considered. Eringen's nonlocal elasticity theory is used to account for the effects of the small length scale. Variations of the lowest frequency parameters with the maximum modal deflection of the embedded MWCNT are obtained using the differential quadrature method in conjunction with a direct iterative approach.

Novel Compact Bandpass Filter Based on Folded Half Mode Substrate Integrated Waveguide Cavities

  • Gong, Ke;Hong, Wei;Chen, Jixin;Tang, Hongjun;Hou, Debin;Zhang, Yan
    • Journal of electromagnetic engineering and science
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    • v.10 no.3
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    • pp.179-182
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    • 2010
  • This paper proposed a novel compact bandpass filter with folded half mode substrate integrated waveguide (FHMSIW) cavities using two-layer printed circuit board(PCB) process. The area of the FHMSIW filter is reduced by nearly 50 % and 75 % compared with half mode substrate integrated waveguide(HMSIW) filter and substrate integrated waveguide(SIW) filter, respectively. A four-pole Chebyshev FHMSIW bandpass filter at C-band has been designed, simulated and fabricated. Measured results are presented and found to agree with the full-wave simulated results by using Ansoft HFSS. The filter shows good performance and compact size.

Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus)

  • Bao, Wenlei;Hao, Xiyan;Zheng, Xu;Liang, Yan;Chen, Yuhao;Wang, Yanfeng;Wang, Zhigang
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.11
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    • pp.1644-1650
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    • 2013
  • Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.

Functional roles of Tryptophan residues in diketoreductase from Acinetobacter baylyi

  • Huang, Yan;Lu, Zhuo;Ma, Min;Liu, Nan;Chen, Yijun
    • BMB Reports
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    • v.45 no.8
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    • pp.452-457
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    • 2012
  • Diketoreductase (DKR) from Acinetobacter baylyi contains two tryptophan residues at positions 149 and 222. Trp-149 and Trp-222 are located along the entry path of substrate into active site and at the dimer interface of DKR, respectively. Single and double substitutions of these positions were generated to probe the roles of tryptophan residues. After replacing Trp with Ala and Phe, biochemical and biophysical characteristics of the mutants were thoroughly investigated. Enzyme activity and substrate binding affinity of W149A and W149F were remarkably decreased, suggesting that Trp-149 regulates the position of substrate at the binding site. Meanwhile, enzyme activity of W222F was increased by 1.7-fold while W222A was completely inactive. In addition to lower thermostability of Trp-222 mutants, molecular modeling of the mutants revealed that Trp-222 is vital to protein folding and dimerization of the enzyme.