• Title/Summary/Keyword: Chemical transformation

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Modified LOGIT(MLOGIT) Transformation: Prediction of $IC_{50}$ Value from Two Arbitrary Concentration Data

  • 유성은;차옥자
    • Bulletin of the Korean Chemical Society
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    • v.16 no.2
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    • pp.110-112
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    • 1995
  • A LOGIT transformation is a method to estimate IC50 values with two arbitrary concentration data when complete dose response curves(DRCs) are not available. We propose a modified LOGIT transformation (MLOGIT) which predicts IC50 values more accurately than the conventional LOGIT method.

Generalization of Keesom Transformation in Multipole-Multipole Interaction Potentials

  • 이천우
    • Bulletin of the Korean Chemical Society
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    • v.16 no.10
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    • pp.952-957
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    • 1995
  • In order to ease the treatment of anisotropic potential when developing the variational RRKM theory, we applied Fano-Racah's recoupling theory to the multipole-multipole interaction, resulting in the great simplification of the anisotropic potentials. The treatment appears as a generalization of Keesom transformation in case of dipole-dipole interaction and provides us with great insights to the characteristics of tensorial interactions in the multipole-multipole interaction system.

Efficient Transformation of Klebsiella oxytoca by Electroporation

  • Ki Jun Jeong;Hyu
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.1
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    • pp.48-49
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    • 1998
  • A protocol for the transformation of Klebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain high transformation efficiency. The highest efficiency of 1.6$\times$106 transformants per $\mu\textrm{g}$ DNA(pBR322) could be obtained by electroporation of K. oxytoca cells prepared at the OD600 of 0.2 with 1.25$\mu\textrm{g}$ DNA at the filed strength of 2.5kV, the parallel resistance of 200$\Omega$ and capacitance of 25$\mu$F.

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Effect of Sodium Hydrosulfite Solution on Agrobacterium-Mediated Chinese Cabbage Transformation and Transient Expression

  • Park Hee-Sung;Shin Dong-Il
    • Journal of Plant Biotechnology
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    • v.7 no.4
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    • pp.219-223
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    • 2005
  • We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.

A Quantitative Resolution of Overlapped Chromatographic Fractions by Target Transformation Factor Analysis

  • Ihn Chong Lee;Seungwon Kim;Chul Lee;Sang Won Choi
    • Bulletin of the Korean Chemical Society
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    • v.12 no.3
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    • pp.304-309
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    • 1991
  • Target transformation factor analysis is applied to analyze the components of unresolved fraction in the elution curve of La, Pr and Gd which is obtained using the chelate agent- impregnated resin and 0.7 M hydrochloric acid as an eluent. We determined the number of components contributing to the unresolved fractions and verified the presence of suspected components.

Characteristics of Heat Generation during Transormation in Carbon Steels (일반탄소강의 상변태 중 발열 특성 해석)

  • 한흥남
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2000.04a
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    • pp.196-201
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    • 2000
  • A thermodynamic model was developed to analyze the characteristics of the heat generation during transformation of austenite in 0.186wt% and 0.458 wt%. carbon steels. The heat capacity and the heat evolved during transformation were formulated as functions of temperature and chemical composition for ferrite bainite and pearlite. in addition using the transformation dilatometer the transformation heat evolved during cooling was measured and the transformation behavior was observed. It was found that the heat capacity of ferrite was similar to those of pearlite and bainite. The heat capacity of ferrite was greater than that of bainite which was greater than that of pearlite. The molar heat of transformation to pearlite was greater than that to bainite which was greater than that to ferrite. The heats were found to be increased with decreased temperature and increasing the carbon content, It was also observed that the thermodynamic model. The heat of transformation in the higher carbon steel was greater than that in the lower carbon one. This was attributed to the lower transformation temperature and the greater amount of transformed pearlite in the higher carbon steel.

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Physical property control for a batch polymerization reactor

  • Kim, In-Sun;Ahn, Sung-Mo;Rhee, Hyun-Ku
    • 제어로봇시스템학회:학술대회논문집
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    • 1996.10a
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    • pp.263-266
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    • 1996
  • A method to determine an optimal temperature trajectory that guarantees polymer products having controlled molecular weight distribution and desired values of molecular weight is presented. The coordinate transformation method and the optimal control theory are applied to a batch PMMA polymerization system to calculate the optimal temperature trajectory. Coordinate transformation method converts the original fixed-end-point, free-end-time problem to a free-end-point, fixed-end-time problem. The idea is that by making the reactor temperature track the optimal temperature trajectory one may be able to produce polymer products having the prespecified physical property in a minimum time. The on-line control experiments with the PID control algorithm have been conducted to establish the validity of the scheme proposed in this study. The experimental results show that prespecified polymer product could be obtained with tracking the calculated optimal temperature trajectory.

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