• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.2
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• pp.155-161
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• 2005

The inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its commercialization. To understand the extent and causes of this instability, this study was aimed at understanding the variability of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, in Vitis vinifera cell cultures. Therefore, four cell line suspensions of Vitis vinifera L. var. Gamay Freaux, A, B, C and D, originated from the same callus by cell-aggregate cloning, were established with starting anthocyanin contents of $2.73\;\pm\;0.15,\;1.45\;\pm\;0.04,\;0.7\;\pm\;0.024\;and\;0.27\;\pm\;0.04$CV (Color Value)/g-FCW (fresh cell weight), respectively. During weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. Contrary to this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern. The variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient (VC), were 58, 57, 54, and $84\%$ for V. vinifera cell lines A, B, C and D, respectively. Within one subculture, the VCs from 12 replicate flasks for each of 12 independent subcultures were averaged, and found to be $9.7\%$, ranging from 4 to $17\%$. High- and low-producing cell lines, VV05 and VV06, with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities to L-phenylalanine feeding, methyl jasmonate and light irradiation. The low-producing cell line showed greater potential in enhanced the anthocyanin production.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.12
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• pp.371-376
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• 2021

The article analyzes the impact of factors affecting the internal control system of chemical enterprises in Vietnam, providing more empirical evidence on factors affecting the system of internal control. Research data is collected in the form of face-to-face interviews and email interviews with managers and employees working in different positions, managers at all levels, chief accountants, and accountants of 52 chemical enterprises in Vietnam. The survey results collected 310 questionnaires. After eliminating the invalid questionnaires due to many blank cells, the authors chose to use 297 questionnaires. Quantitative research was carried out with SPSS 25 software. Research results show that Business Strategy, Organizational Structure, Organization's perception of the instability of the external environment, and Organizational culture are the factors that positively affect the internal control system of chemical enterprises. This can be explained that, for chemical enterprises, Business strategy, Organizational structure, Organization's perception of the instability of the external environment, and Good organizational culture will positively impact the internal control system of chemical enterprises. Based on the research results, the authors have proposed recommendations to enhance the effectiveness of the internal control system of chemical enterprises in Vietnam, thereby contributing to improving the performance of enterprises in the chemical Vietnamese.

• Nuclear Engineering and Technology
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• v.55 no.1
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• pp.109-118
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• 2023

In this paper, the evaluation method of fluidelastic instability (FEI) of newly designed steam generator tubing in pressurized water reactor (PWR) nuclear power plants is discussed. To obtain the parameters for prediction of the critical velocity of FEI for steam generator tubes, experimental research is carried out, and the design parameters are determined. Using CFD numerical simulation, the tube array scale of the model experiment is determined, and the experimental device is designed. In this paper, 7 groups of experiments with void fractions of 0% (water), 10%, 20%, 50%, 75%, 85% and 95% were carried out. The critical damping ration, fundamental frequency and critical velocity of FEI of tubes in flowing water were measured. Through calculation, the total mass and instability constant of the immersed tube are obtained. The critical damping ration measured in the experiment mainly included two-phase damping and viscous damping, which changed with the change in void fraction from 1.56% to 4.34%. This value can be used in the steam generator design described in this paper and is conservative. By introducing the multiplier of frequency and square root of total mass per unit length, it is found that the difference between the experimental results and the calculated results is less than 1%, which proves the rationality and feasibility of the calculation method of frequency and total mass per unit length in engineering design. Through calculation, the instability constant is greater than 4 when the void fraction is less than 75%, less than 4 when the void fraction exceeds 75% and only 3.04 when the void fraction is 95%.

• Proceedings of the Korean Society of Rheology Conference
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• 2001.09a
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• pp.164.4-165
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• 2001

• Polymer(Korea)
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• v.38 no.2
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• pp.213-219
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• 2014

The rheological characteristics of highly concentrated polymer bonded explosive simulant were studied. Hydroxyl terminated polybutadiene (HTPB) and polyethylene plastomer (Exact) were used as binders. Sugar and Dechlorane particles whose physical properties are similar to research department explosive (RDX) were used as fillers. When HTPB was used, diethyl hexyl adipate (DEHA or DOA) was used as a plasticizer together for some cases. Highly concentrated suspensions were mixed in a batch melt mixer (Rheomixer 600, Haake) and rheological properties were measured by plate-plate and capillary rheometers. Wall slip phenomena, thixotropy with shear hysteresis, and flow instability were investigated as shear rate and amount of fillers changed.

• Korean Chemical Engineering Research
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• v.53 no.5
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• pp.609-613
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• 2015

The onset of instability induced by impulsive spin-down of the rigid-body flow placed in the gap between two coaxial cylinders is analyzed by using the energy method. In the present stability analysis the growth rate of the kinetic energy of the base state and also that of disturbances are taken into consideration. In the present system the primary flow is a transient, laminar one. But for the Reynolds number equal or larger than a certain one, i.e. $Re{\geq}Re_G$ secondary motion sets in, starting at a certain time. For $Re{\geq}Re_G$ the dimensionless critical time to mark the onset of vortex instabilities, ${\tau}_c$, is here presented as a function of the Reynolds number Re and the radius ratio ${\eta}$. For the wide gap case of small ${\eta}$, the transient instability is possible in the range of $Re_G{\leq}Re{\leq}Re_S$. It is found that the predicted ${\tau}_c$-value is much smaller than experimental detection time of first observable secondary motion. It seems evident that small disturbances initiated at ${\tau}_c$ require some growth period until they are detected experimentally.

• Korean Chemical Engineering Research
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• v.59 no.1
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• pp.138-151
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• 2021

The effect of a reactant ratio on the growth of a buoyancy-driven instability in an irreversible A+B→C reaction system is analyzed theoretically and numerically. Taking a non-stoichiometric reactant ratio into account, new linear stability equations are derived without the quasi-steady state assumption (QSSA) and solved analytically. It is found that the main parameters to explain the present system are the Damköhler number, the dimensionless density difference of chemical species and the ratio of reactants. The present initial grow rate analysis without QSSA shows that the system is initially unconditionally stable regardless of the parameter values; however, the previous initial growth rate analysis based on the QSSA predicted the system is unstable if the system is physically unstable. For time evolving cases, the present growth rates obtained from the spectral analysis and pseudo-spectral method support each other, but quite differently from that obtained under the conventional QSSA. Adopting the result of the linear stability analysis as an initial condition, fully nonlinear direct numerical simulations are conducted. Both the linear analysis and the nonlinear simulation show that the reactant ratio plays an important role in the onset and the growth of the instability motion.

• Proceedings of the Korean Society of Rheology Conference
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• 2006.06a
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• pp.165-169
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• 2006

• Journal of Mechanical Science and Technology
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• v.18 no.6
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• pp.1010-1018
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• 2004

This study presents the methodological aspects of combustion instability modeling and provides the numerical results of the model (sub-scale) combustion chamber, regarding geometrical dimensions and operating conditions, which are for determining the combustion stability boundaries using the model chamber. An approach to determine the stability limits and acoustic characteristics of injectors is described intensively. Procedures for extrapolation of the model operating parameters to the actual conditions are presented, which allow the hot-fire test data to be presented by parameters of the combustion chamber pressure and mixture (oxidizer/fuel) ratio, which are customary for designers. Tests with the model chamber, based on the suggested scaling method, are far more cost-effective than with the actual (full-scale) chamber and useful for injector screening at the initial stage of the combustor development in a viewpoint of combustion instabilities.

• 한국연소학회:학술대회논문집
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• 2005.10a
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• pp.294-301
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• 2005

The instabilities in rocket engines and gas turbine combustors due to the interaction between the fluid flow (acoustics) and the heat transfer (thermal energy) are called thermoacoustic or combustion instabilities. Almost all analysis assumes constant hot section temperature for Modern mathematical analysis of acoustic oscillations in Rijke type devices. However, it is impossible to predict whether a system is stable or not because the flame or heater response model can have a dramatic effect on predicted growth rates. In this study, A standard ${\kappa}-{\varepsilon}$ turbulent model and hybrid combustion model(eddy breakup model and chemical reaction) were used. After steady solution was gotten, unsteady calculation is simulated by perturbating on pressure boundary. As a result, we obtained the relationship of equivalence ratio and frequency by numerical simulation, and they are comparable to the experimental result. In addition, in spite of these results, there are limitations of using turbulent and combustion model in simulation method of thermoacoutic instability