• Journal of Trauma and Injury
• /
• v.18 no.1
• /
• pp.53-63
• /
• 2005

Purpose: This study was designed to determine if methylene blue inhibited the lipid peroxidation, the production of NO, and the gene expression of iNOS in acute lung injury induced by paraquat and if the inhibitory effect was dose dependent. Methods: Female Sprague-Dawley rats were divided into four groups: the control group, the group treated with paraquat only, the group treated with paraquat and a low dose of methylene blue (2 mg/kg), and the group treated with paraquat and a high dose of methylene blue (20 mg/kg). Methylene blue was administered via the jugular vein 1 h after paraquat administration, and animals were sacrificed 6 and 24 h after paraquat administration. Malondialdehyde (MDA) as lipid peroxidation, reduced glutathione (GSH) as an antioxidant defense, the plasma NO concentration, and the expression of iNOS mRNA in the lung tissue were measured Results: Lung MDA contents decreased, with no significant difference between the methylene-blue groups and the paraquat-only group. Lung GSH contents were significantly elevated at 24 h in the methylene-blue groups compared with the paraquat-only group. Plasma NO concentrations were significantly reduced at 6 and 24 h in the methylene-blue groups compared with the paraquat-only group. There was also a significant decrease in the plasma NO concentration at 6 h in the high-dose methylene-blue group compared with the low-dose methylene-blue group. The expression of iNOS mRNA in the lung tissue was slightly decreased in the methylene-blue groups. It was also markedly increased at 24 h in the paraquat-only group compared with the methylene-blue groups. The gene expression was relatively decreased in the high-dose methylene-blue group compared with the low-dose methylene-blue group. Conclusion: This study suggests that methylene blue has an inhibitory effect on the plasma NO concentration and the expression of iNOS mRNA in lung injury induced by paraquat. No inhibitory effect of methylene blue on lipid peroxidation or dose-dependent inhibitory effects were clearly shown.

• Clinical and Experimental Reproductive Medicine
• /
• v.31 no.1
• /
• pp.1-7
• /
• 2004

연구 목적: 본 연구의 목적은 마우스에서 배양액의 용량이 배반포 배 형성과 세포수에 미치는 영향을 조사하기 위하여 실시하였다. 연구 재료 및 방법: $3{\sim}4$주령 ICR 암마우스에게 48시간 간격으로 5 IU PMSG와 hCG 주사 후 (hCG 주사 후 수컷과 동숙) $46{\sim}50$시간에 난관으로부터 총 138개의 2-세포기 배를 회수하여 2 ml (group I) 또는 $50{\mu}l$ (group II)의 배양액 (Dulbecco's Modified Eagle Medium + 20% human follicular fluid)에서 72시간 동안 배반포기까지 배양하였다. 배반포 배는 zona-intact (ZiB)와 zona-escape (ZeB)로 등급을 구분하고 나서, propidium iodide와 bisbenzimide를 이용한 differential staining 방법으로 염색하여 평균 세포수, 내세포괴(ICM) 세포수, 영양배엽(TE) 세포수, 총 세포수에 대한 ICM의 비율 (%ICM) 및 ICM:TE 비율을 조사하였다. 결과에 대한 유의성 검정은 $X^2$ test와 t-test를 이용하였으며, p<0.05일 때 통계적인 차이가 있는 것으로 하였다. 결 과: Group I과 II에서, 총 배반포 ($62.3{\pm}20.7%$ vs. $63.8{\pm}22.9%$), ZiB ($31.9{\pm}24.0%$ vs. $30.4{\pm}18.2%$)와 ZeB 형성율 ($30.4{\pm}20.8%$ vs. $33.3{\pm}22.3%$)은 차이가 없었다. 87개의 배반포 배를 염색 시도하였는데, 명확하게 differential staining된 41개의 배반포 배만을 대상으로 세포수를 조사하였다. 평균 세포수 ($61.6{\pm}19.5$ vs. $63.7{\pm}26.8$), ICM 세포수 ($13.0{\pm}10.6$ vs. $12.8{\pm}10.5$), TE 세포수 ($49.0{\pm}19.0$ vs. $47.8{\pm}18.7$), %ICM ($21.0{\pm}12.6%$ vs. $21.1{\pm}13.2%$) 및 ICM:TE 비율 (1:$3.77{\pm}4.9$ vs. 1:$3.72{\pm}4.8$)에서도 group I과 II에서 차이가 없었다. 결 론: 마우스에서 배 발생 능력의 척도로 쓰이는 배반포 배 형성율 배반포 배의 등급 세포수 및 %ICM 등이 20% 난포액을 첨가한 MEM 배양액의 용량에 따라 영향을 받지 않았다.

• Journal of Korean Society of Environmental Engineers
• /
• v.28 no.9
• /
• pp.911-916
• /
• 2006

The influence of substrate concentration and hydraulic retention time(HRT) on the hydrogen production by anaerobic microflora was investigated by conducting three series of continuous experiments the individual influences of substrate concentration and HRT. In series I, substrate concentration was increased from 3 to 27 g-glucose/L keeping HRT at 8 hr. Series II and III carried out same condition with series I at HRT of 16 hr and 24 hr, respectively. The effects of HRT and substrate concentration on the hydrogen production yield were analyzed by quadratic model. The maximum hydrogen production yield of 2.05 mol $H_2/mol$ glucose was found at the HRT of 9.6 hr and the substrate concentration of 15.4 g/L. The relationship between HRT and substrate concentration on hydrogen production yield as displayed a saddle shape in the response surface plot. Optimum HRT and substrate concentration are observed at in the range of 5 and 14 hr, at between 13 and 17 g/L, respectively, for the hydrogen production yield being 2 mol $H_2/mol$ glucose. The concentrations of organic acids increased with the increase of the amount of glucose consumption. Acetic acid and butyric acid were the main by-products from the glucose degradation.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.34 no.3
• /
• pp.274-282
• /
• 1998

This study deals with sinking resistance for purse seine, in the case of different d/l, ratio of twine diameter and leg length. Experiments carried out on the six types simplified reduced model seines which were made of knotless netting. The nettings were woven in different leg length 4.3, 5.0, 5.5, 6.0, 6.6 and 7.7mm of polyester 28 tex two threads two -ply twine, and each of the seines were named I, II, III, IV, V and Ⅵ seine. Dimension of seine models were 450cm for corkline and 85cm for seine depth, each seines rigged up 160g of float for a floatline and 50g (underwater weight) of lead for a leadline. Experiments were measured in the observation channel of a flume tank at the static conditions Sinking motion was recorded by the two sets TV-camera for VTR which were placed in top and side of the model seine, and reading coordinate carried out by the video digitization system. An analysis were calculated out by simultaneous differential equations for numerical method by Runge - Kutta - Gill sub - routine. The results obtained were as follows: 1. Average sinking speed of seine of seine margin was fastest for Ⅵ seine followed by V, IV, III, II and I seines. 2. The coefficient of resistance for a seine wall was depended upon the ratio of d/l : KD =0.081 (d/l )-0.5 3. The coefficient of resistance for netting bundle was not depended upon the ratio of d/l :CR = 0.91 (), d : Twine diameter, l : Leg length, : Density of netting materals, $\omega$ : Density of water

• Korean Journal of Weed Science
• /
• v.9 no.3
• /
• pp.201-213
• /
• 1989

About 233 weed species belonging to 54 families mainly occurring in summer were observed in cultivated land and its surrounding area in Cheju Island. The compositae was the most widely occurring family covering 41 weed species, followed by 28 species in gramineae, 14 in labiatae, 13 in leguminasae and 12 in polygonaceae etc.. In terms of the lands classified about 126 species in 39 families were observed in upland ared and 38 species in 20 families occurred in lowland area, 108 species in 36 families in orchard and 214 species in 50 families observed in non-cultivated area. The most dominant weed species in Cheju Island were Portulaca oleracea, Digitaria sanguinalis in both the upland and orchard, Monochoria vaginalis, Cyperus amuricus in lowland, and Digitaria sanguinalis, Artemisia princeps, Partulaca oleracea in non-cultivated land area. There were two communities in upland area, such as Phyllanthus urinaria, Siegesbeckia orinexitalis etc., and Cephalonaplos segetum, Mosla dianthera etc.. The lowland area composed of one major community like Onenanthe javanica, and Eleocharis acicularis. The orchard consisted of the communities of Stellaria aquatics, Viola mandshurica etc., and Justicia procumbens, Varedellia crustacea etc.. The non-cultivated land composed of two major communities like Amplicarpaed edgeworthii, Lactuca indica etc., and Clematis appiiforia, Aster hispidus etc..

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.6 no.2
• /
• pp.103-110
• /
• 1986

The experiments were carried out to study the influence of meteorological factors and cutting management on dry matter accumulation and net energy value in orchardgrass (Dactlylis glomerata L.) cv. Potomac and Baraula, perennial ryegrass (Lolium perenne L.) cv. Reveille and Semperweide and meadow fescue (Festuca pratensis Huds.) cv. Cosmos 11 and N.F.G.. The field trials were designed as a split plot design with three cutting regimes of 6-7 cuts at grazing stage, 4-5 cuts at silage stage and 3 cuts at hat stage in Korea and West Germany from 1975 to 1979. The results obtained are summarized as follows: 1. Productivity of orchardgrass, perennial ryegrass and meadow fescue were mainly affected by cutting systems and meteorological factors, especially air temperature, rainfalls, solar radiation and their interactions. In West Germany, cutting frequency was to be found asan most important factor influenced to dry matter yield and net energy value. 2. Orchardgrass, taken as average of all experimental sites in Korea, produced high yield of 875 kg/10 a in dry matter, which was as much as 32% and 27% higher than those of perennial ryegrass and meadow fescue, respectively. The annual dry matter yields of orchardgrass from 1976 to 1977 were shown a little variation. Dry matter yields in Freising and Braunschweig in West Germany were increased in all grass species continuously. 3. Orchardgrass, perennial ryegrass and meadow fescue showed different response to cutting frequency. The highest dry matter yields were found under 3 cuts at hay stage for orchardgrass and 4-5 cuts at silage stage for perennial ryegrass and meadow fescue. In West Germany, dry matter yields, as average of all grass species under different cutting systems, were 1326 kg, 1175 kg and 1098 kg/10a for 3 cuts, 4-5 cuts and 6-7 cuts, respectively. 4. Chemical composition and net energy concentration of temperate grasses were influenced by cutting managements. The highest yields of digestible crude protein were obtained under 6-7 cuts at grazing stage both in Korea and West Germany. In net energy yields, 3 cutting system produced the highest yield with 694 (orchardgrass), 665 (perennial ryegrass) an 623 kStE/10 a (meadow fescue). However, frequent cutting at grazing and silage stage produced higher yields than 3 cuts at hay stage in Cheju, Suweon and Taekwalyong.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.42 no.2
• /
• pp.116-122
• /
• 2009

This study was conducted to compare the food quality of commercial salt-fermented silver-stripe round herring (SFS) to that of commercial salt-fermented anchovy (SFA). The contents of moisture and crude protein of SFS were higher than those of SFA. However, the crude lipid and crude ash contents of SFS were lower than those of SFA. The salinity of SFS was 13.2%, which was lower than that of SFA. The taste value of SFS was 148.1, which was higher than that of SFA. The total amino acid content of SFS was 18.1 g/100 g and its major amino acids were glutamic acid (9.5%) and aspartic acid (8.1%). The contents of calcium, phosphorus and iron in SFS were 647 mg/100 g, 363 mg/100 g and 4.1 mg/100 g, respectively. Twenty six types of fatty acids were detected in SFS and the major components were 16:0, 18:0, 18:1n-9 and 22:6n-3. The angiotensin I converting enzyme (ACE) inhibiting and the antioxidative abilities of SFS were 50.0% and 1.15, respectively, which were similar to those of SFA. Sensory evaluation revealed that SFS had a superior taste and color to SFS, while to the flavor of two products was similar.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• v.43 no.6 s.312
• /
• pp.52-59
• /
• 2006

Recently, the internet has seen tremendous growth with plenty of enriched information due to an increasing number of specialized personal interests and popularizations of private cyber space called, blog. Many of today's blog provide internet users, RSS, which is also hewn as the syndication technology. It enables blog users to receive update automatically by registering their RSS channel address with RSS aggregator. In other words, it keeps internet users wasting their time checking back the web site for update. This paper propose the ways to manage RSS Channel Searching Crawler and collected RSS Channels for internet users to search for a specific RSS channel of their want without any obstacles. At the same time. This paper proposes RSS channel ranking based on user popularity. So, we focus on an idea of adding index to information and web update for users to receive appropriate information according to user property.

• Journal of Animal Science and Technology
• /
• v.47 no.3
• /
• pp.317-324
• /
• 2005

A method for sex determination of pigs was examined using polymerase chain reaction(PCR). Sex determining region Y(SRY) gene encoded on Y chromosome plays a key role for primary male development. Zinc finger X-Y(ZFX-ZFY) gene, one of the X-V homology gene group was found on the X and Y chromosomes, respectively, We tested for molecular sexing by amplification patterns of SRY and ZF genes. Genomic DNAs from various resources including porcine hairs and semen collected from domestic pig breeds and native pigs was used for PCR assay of each gene. The amplified products for porcine SRY gene were yielded only in males but not in females. On the other hand, two differential patterns were observed in amplification of ZF gene reflecting the chromosomal dimorphism by a length polymorphism between X and Y chromosomes. Of both, a common band was detected in all individuals tested so that this band might be amplified from ZFX gene as a PCR template, but another is specific for males indicated that from ZFY. The result of PCR assay provides identical information to that from investigation of phenotypic genders of the pigs tested. We suggest that this PCR strategy to determine porcine sexes using comparison of the amplification patterns of the SRY gene specific for Y chromosome and the dimorphic ZF gene between X and Y chromosomes may be a rapid and precise method for discrimination of two sexes and applied to DNA analysis of small samples such as embryonic blastomere, semen, and hairs.

• Korean Journal of Veterinary Research
• /
• v.43 no.3
• /
• pp.485-492
• /
• 2003

The pathological mechanism of impaired spermatogenesis after vasectomy has not been completely investigated. In this study, we examined pathological changes of the testis and the Fas-Fas ligand (FasL) mediated signaling pathway in apoptotic germ cell death after vasectomy in rats. Ten-weeks old Sprague-Dawley rats were underwent bilateral vasectomy and sacrificed after 1 day, 2 days, 3 days, 5 days, 1 week, 2 weeks, and 4 weeks of surgery and the testes were removed. Histopathological evaluation of spermatogenesis was performed by hematoxylin-eosin and periodic acid-Schiff-hematoxylin staining. To elucidate the pathophysiology of seminiferous tubule damage, terminal dUTP nick end labeling staining, electrophoresis assay of DNA fragmentation, and Western blotting analysis for Fas-FasL were performed. Relative weights of testes were decreased from 5 days after vasectomy. Germ cell degeneration were first found in the spermatogonia and spermatocytes at stages I-VI, and XII-XIV seminiferous tubules. Mean incidence of apoptotic germ cells after vasectomy progressively increased to peak in 5 days, and then gradually decreased to the control levels in 2 weeks after vasectomy. The expression of Fas-FasL reached maximum level at 5 days after vasectomy and then declined. In conclusion, impaired spermatogenesis after vasectomy associated with an increase in germ cell apoptasis, which is partly mediated by the activation of Fas-FasL.