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Influence of Substrate Concentration and Hydraulic Retention Time on the Hydrogen Production Using Anaerobic Microflora  

Ko, In-Beom (Department of Civil and Environmental Engineering, KAIST)
Shin, Hang-Sik (Department of Civil and Environmental Engineering, KAIST)
Lee, Yong-Doo (Major of Civil and Environmental Engineering, Cheju National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.9, 2006 , pp. 911-916 More about this Journal
Abstract
The influence of substrate concentration and hydraulic retention time(HRT) on the hydrogen production by anaerobic microflora was investigated by conducting three series of continuous experiments the individual influences of substrate concentration and HRT. In series I, substrate concentration was increased from 3 to 27 g-glucose/L keeping HRT at 8 hr. Series II and III carried out same condition with series I at HRT of 16 hr and 24 hr, respectively. The effects of HRT and substrate concentration on the hydrogen production yield were analyzed by quadratic model. The maximum hydrogen production yield of 2.05 mol $H_2/mol$ glucose was found at the HRT of 9.6 hr and the substrate concentration of 15.4 g/L. The relationship between HRT and substrate concentration on hydrogen production yield as displayed a saddle shape in the response surface plot. Optimum HRT and substrate concentration are observed at in the range of 5 and 14 hr, at between 13 and 17 g/L, respectively, for the hydrogen production yield being 2 mol $H_2/mol$ glucose. The concentrations of organic acids increased with the increase of the amount of glucose consumption. Acetic acid and butyric acid were the main by-products from the glucose degradation.
Keywords
Anaerobic Microflora; Hydrogen Production; HRT; Organic Acid;
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