• Archives of Pharmacal Research
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• 제28권9호
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• pp.1047-1052
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• 2005

Two phenolic glucosides, eutigoside Band eutigoside C were isolated from the fresh leaves of Eurya emarginata. These two phenolic glucosides exerted a significant inhibitory effect on the growth of HL-60 promyelocytic leukemia cells. Furthermore, when the HL-60 cells were treated with eutigoside C, several apoptotic characteristics such as DNA fragmentation, morphologic changes, and increase of the population of sub-G1 hypodiploid cells were observed. In order to understand the mechanism of apoptosis induction by eutigoside C, we examined the changes of Bcl-2 and Bax expression levels. The eutigoside C reduced BcI-2 protein and mRNA levels, but slightly increased Bax protein and mRNA levels in a time-dependent manner. When we examined the activation of caspase-3, an effector of apoptosis, the eutigoside C increased the expression of active form (19-kDa) of caspase-3 and the increase of their activities was demonstrated by the cleavage of poly (ADP-ribose) polymerase, a substrate of caspase-3, to 85-kDa. The results suggest that the inhibitory effect of eutigoside C from E. emarginata on the growth of HL-60 appears to arise from the induction of apoptosis via the down-regulation of BcI-2 and the activation of caspase.

• Applied Biological Chemistry
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• 제38권2호
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• pp.118-122
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• 1995

Escherichia coli의 오르니틴 트란스카바밀라제는 오르니틴과 카바밀인산으로부터 시트룰린의 합성을 촉진시키는 효소이다. 이 효소의 기능과 구조와의 상관관계, 반응메카니즘 등 생화학적 연구를 하기 위하여 대량의 효소를 추출할 필요가 있다. 본 연구는 오르니틴 트란스카바밀라제의 대량생산 시스템을 확립하기 위하여 E. coli argI 유전자를 E. coli $DH5{\alpha}$ 세포의 염색체 DNA를 추출한 후에 PCR 방법으로 증폭시켜 얻었다. 증폭된 argI 유전자를 단핵생물 단백질 발현벡터인 pKK223-3에 접합시킨 후, 오르니틴 트란스카바밀라제가 존재하지 않은 E. coli TB2 세포에 클로닝 시켰다. 이 세포로부터 생산된 오르니틴 트란스카바밀라제는 암모늄염에 의한 분할, 열변성, 크로마토그래피 등을 사용하여 순수하게 분리하였다. SDS 단백질 전기영동 결과 약 38 kDa 크기의 효소가 순수하게 얻어졌다. 반응속도론적 실험결과 $K_{cat}$은 $1{\times}10^5m^{-1}$, $K_M$은 오르니틴에 대하여는 0.35 mM, 카바밀인산에 관하여는 0.06 mM이 각각 얻어졌다. 이 결과는 야생형 오르니틴 트란스카바밀라제의 반응속도 인자들과 비슷한 값이다. 본 연구는 이들 결과로부터 오르니틴 트란스카바밀라제의 기능을 하는 E. coli argI 유전자가 클로닝 되었음을 확인하였다.

• 생약학회지
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• 제37권2호통권145호
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• pp.74-80
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• 2006

We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform $(CHCI_3)$ fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of $100\;{\mu}g/ml$ in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of $50\;{\mu}g/ml$ in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).

• 한국임상수의학회지
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• 제20권3호
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• pp.312-316
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• 2003

This study was performed to investigate the reverse effects of Jen Chung(Ren Thong, Shui Gou, GV-26) electroacupuncture after tiletamime-zolazepam administration in dogs. Seven healthy dogs ranging in weigh from 3.5 to 6.5 kg were used in this experiment. The treatment group was electrostimulated to Jen Chung(+) and Su Liao(Shan Gen, GV-25, -) for 20 minutes after 10 minutes of anesthesia. The control group I was anesthetized with tiletamine-zolazepam. The control group II was electrostimulated to nonacupuncture point for 20 minutes after 10 minutes of anesthesia. Various parameters were evaluated including the onset and recovery time of anesthesia, heart rates, body temperature, respiratory rates and electrocardiogram. The recovery time of the treatment group was shorter than that of the control group I and the control group II(p < 0.05). The treatment group had an increase in the heart rate from 20 minutes to 30 minutes after administration of tiletamime-zolazepam compared to the control group I and control group II(p < 0.05). The decrease in the body temperature was observed in all groups, but the body temperature of the treatment group was higher than the body temperature of the other groups. Respiratory rates gradually increased in all groups, but the treatment group had an increase in the respiratory rates from 20 minutes to 30 minutes after administration of tiletamime-zolazepam compared to the other groups. In the three groups, there was no specific finding on the electrocardiogram. In the result, the electroacupuncture on Jen Chung in dogs was effective for the reversed effects the tiletamine-zolazepam anesthesia 20 minutes after induction.

• 한국균학회지
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• 제20권4호
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• pp.360-368
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• 1992

1959년부터 1990년까지 발표(發表)된 제주도산(濟州道産) 버섯을 재정리(再整理)한 결과 총(總) 1문(門), 2아문(亞門), 4강(綱), 3아강(亞綱). 14목(目), 41과(科), 96속(屬), 186종(種)으로 나타났다. 그러나 5속(屬) 16종(種)은 이명(異名)의 이중기록(二重記錄)으로 인하여 목록(目錄)에는 101속(屬) 202종(種)으로 나타나 있다.

• 한국임상수의학회지
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• 제24권4호
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• pp.550-556
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• 2007

The purpose of this study is to assess healing effect of the green tea on Staphylococcus contaminated wound. On the back of 7 dogs, 6 full-thickness skin wounds($2cm{\times}2cm$) were made and Staphylococcus intermedius was inoculated. Each wound was applied with the 2%(experimental group I), 1%(experimental group II) extract of green tea and normal saline(control group I) after inoculation. The wound contraction rate of the experimental group I and II was higher than that of the control group in entire period. And from the $6^{th}\;to\;22^{nd}$ day, the wound contraction rate of the experimental group I and II was significantly higher than that of the control group(p<0.05). The occupation rate of wound healing in the experimental group I was higher than that of the control group from $0\;to\;9^{th}$ day except from $1^{st}\;to\;2^{nd}$ day and also higher than that of the experimental group II from $0\;to\;3^{rd}$ day except from $1^{st}\;to\;2^{nd}$ day. And the occupation rate of wound healing in the experimental group II was higher than that of the control group from $1^{st}\;to\;8^{th}$ day except from $2^{nd}\;to\;3^{rd}$ day. According to concentration of green tea extract increased, the wound contracted more vigorously in earlier period. The tensile strength of the experimental group I and II was significantly higher than that of the control group(p<0.05). And that of the experimental group I was also higher than in the experimental group II(p<0.05). The number of S. intermedius in the experimental group I and II was decreased more rapidly than that of the control group. From the $4^{th}$ to the $11^{th}$ day, the number of S. intermedius in the experimental group I was significantly lower than that of the experimental group II and the control group. And from the $4^{th}$ to the $7^{th}$ day, the number of S. intermedius in the experimental group was significantly lower than that of the control group. The histopathological findings of the experimental group I and II were similar to those of the control group on the $1^{st}\;and\;4^{th}$ day. On $14^{th}$ day, reepithelialization was completed in the experimental group I and II except the control group. Higher wound contraction rate, better bacteriocidal action, higher tensile strength and better histopathological findings were observed in the experimental group I and II than in the control group. These results suggest that the topical application of green tea extract can promote contaminated wound healing in dogs.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.39.1-39
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• 2001

Impedance tomography (EIT) is a relatively new imaging modality in which the internal impedivity distribution is reconstructed based on the known sets of injected currents through the electrodes and induced voltages on the surface of the object. We describe a dynamic EIT imaging technique for the case where the resistivity distribution inside the object changes rapidly within the time taken to acquire a full set of independent measurement data, In doing so, the inverse problem is treated as the nonlinear state estimation problem and the unknown state (resistivity) is estimated with the aid of extended Kalman filter in a minimum mean square error sense. In particular, additional electrodes are attached to the known internal structure of the object ...

• 한국패류학회:학술대회논문집
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• 한국패류학회 1999년도 추계학술발표대회요약집(한국패류학회)
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• pp.12-12
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• 1999

• Preventive Nutrition and Food Science
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• 제10권3호
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• pp.239-243
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• 2005

Angiotensin I-converting enzyme (ACE) inhibitory activities of elk antler hydrolysates prepared with three kinds of proteases, pepsin, trypsin and $\alpha-chymotrypsin$, were investigated. The ACE inhibitory activity of the pepsinolytic hydrolysate was the highest with an $IC_{50}$ value of $9.3\mu g/mL.$ In addition, three kinds of pepsinolytic hydrolysates with relatively high molecular weights (over 10,000 Da), medium molecular weights (5,000 to 10,000 Da), and low molecular weights (below 5,000 Da) were fractionated using an ultrafiltration membrane system. The below 5,000 Da hydrolysate exhibited the highest ACE inhibitory activity. These results indicate that the pepsinolytic hydrolysates of elk velvet antler could be a good source of peptides with ACE inhibitory activity.

• 대한수의학회지
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• 제35권4호
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• pp.735-741
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• 1995

The present study was conducted to investigate the incidence of equine herpesvirus 1(EHV-1) infection in Korean horses by cell EUSA and serum neutralization test A significant level of antibodies to EHV-1 was detected from 539(82.8%) by cell EUSA and from 524 horses(80.5%) by SNT indicating that EHV-1 infection in Korean horses are widely spreaded throughout the country.