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Anti-inflammatory Effect of Unripe Fruit of Citrus grandis Osbeck in RAW 264.7 and HaCaT Cells  

Lee, Hye-Ja (Department of Pharmacology, College of Medicine, Cheju National University)
Kang, Gyeoung-Jin (Department of Pharmacology, College of Medicine, Cheju National University)
Yoon, Weon-Jong (Department of Pharmacology, College of Medicine, Cheju National University)
Kang, Hee-Kyoung (Department of Pharmacology, College of Medicine, Cheju National University)
Kim, Young-Suk (Department of Food and Nutritional Sciences, Ewha Womans University)
Kim, So-Mi (Faculty of Biotechnology, College of Applied Life Science, Cheju National University)
Yoo, Eun-Sook (Department of Pharmacology, College of Medicine, Cheju National University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.2, 2006 , pp. 74-80 More about this Journal
Abstract
We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform $(CHCI_3)$ fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of $100\;{\mu}g/ml$ in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of $50\;{\mu}g/ml$ in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).
Keywords
Citrus grandis Osbeck; IL-6; iNOS; COX; TARC; MDC;
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