This study aims to report the results for the analysis of the growth environment by applying smart farm technology to "Chunchu No 2" farmers in order to develop an optimal growth model for precision cultivation of bottle-grown oyster mushrooms. The temperature, humidity, carbon dioxide concentration, and illumination data were collected and analyzed using an environmental sensor installed to obtain growth environment data from the oyster mushroom cultivator. Analysis of the collected temperature data revealed that the temperature at the time of granulation was $19.5^{\circ}C$ after scraping, and the mushroom was generated and maintained at about $21^{\circ}C$ until the bottle was flipped. When the fruiting body grew and approached harvest time, mushrooms were harvested while maintaining the temperature between $14^{\circ}C$ and $18^{\circ}C$. The humidity was maintained at almost 100% during the complete growth stage. Carbon dioxide concentration gradually increased until 3 days after the beginning of cultivation, and then increased rapidly to almost 5,500 ppm. From the 6th day, carbon dioxide concentration was gradually decreased through ventilation and was maintained at 1,600 ppm during harvest. Light intensity of 8 lux was irradiated up to day 6 after seeding, and growth was then continued while periodically irradiating 4 lux light. The fruiting body characteristics of "Chunchu No 2" cultivated in the farmhouse were as follows: pileus diameter of 26.5 mm and thickness of 4.9 mm, stipe thickness of 8.9 mm, and length of 68.7 mm. The fruiting body yield was 166.8 g/850 ml, and the individual weight was 12.8 g/10 units.
This study was conducted to evaluate the growth characteristics of lettuce (Lactuca sativa L.) as affected by artificial light sources and different growing media in a closed-type plant production system (CPPS). The lettuce seeds were sown in the 128-cell plug tray filled with 5 different growing media such as urethane sponge (US), rock-wool (RW), Q-plug (QP), TP-S2 (TP) and PU-7B (PU). The germination rate of lettuce seeds was examined during 12 days after sowing. On the 13 days after sowing, the lettuce seedlings were transplanted in a CPPS with temperature $25{\pm}1^{\circ}C$ and nutrient solution (EC $2.0dS{\cdot}m^{-1}$, pH 6.5) using recirculating deep floating technique system. The light sources were set with FL (fluorescent lamps) and combined RB LEDs (red : blue = 7 : 3) with $150{\pm}10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and a photoperiod of 14/10 hours (light/dark). The initial germination rate of lettuce was the highest in TP. The final germination and mean daily germination were the significantly highest in RW, QP and TP. The plant height, leaf length, leaf width, leaf area, and fresh and dry weights of shoot were the greatest in QP irradiated with RB LED. The number of leaves, fresh and dry weights of root and SPAD were the greatest in QP and TP irradiated with RB LED. The root length was the longest in TP irradiated with RB LED. Therefore, these results indicate that RB LED was effective for the growth of lettuce and it was also found that the QP and TP were effective for the germination and growth of lettuce in a CPPS. In addition, we confirmed the applicability of the newly developed growing medium TP for the lettuce production in a CPPS.
Kim, Daehyuk;Shin, Hong-Ryeol;Choi, Min-bum;Choi, Young-Jin;Choi, Byoung-Ju;Seo, Gwang-Ho;Kwon, Seok-Jae;Kang, Boonsoon
Journal of Korean Society of Coastal and Ocean Engineers
/
v.32
no.6
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pp.587-601
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2020
The ocean circulation was simulated in the East Sea and Ulleungdo-Dokdo region using ROMS (Regional Ocean Modeling System) model. By adopting the East Sea 3 km model and the HYCOM 9 km data, Ulleungdo 1 km model and Ulleungdo-Dokdo 300 m model were constructed with one-way grid nesting method. During the model development, a correction method was proposed for the distortion of the open boundary data which may be caused by the bathymetry data difference between the mother and child models and the interpolation/extrapolation method. Using this model, a super-high resolution ocean circulation with a horizontal resolution of 300 m near the Ulleungdo and Dokdo region was simulated for year 2018. In spite of applying the same conditions except for the initial and boundary data, the numerical models result indicated significantly different characteristics in the study area. Therefore, these results were compared and verified by using the surface current data estimated by satellites altimeter data and temperature data from NIFS (National Institute of Fisheries Science). They suggest that in general, the improvement of the one-way grid nesting with the HYCOM data on RMSE, Mean Bias, Pattern correlation and Vector correlation is greater in 300 m model than in the 1 km model. However, the nesting results of using East Sea 3 km model showed that simulations of the 1 km model were better than 300 m model. The models better resolved distinct ridge/trough structures of isotherms in the vertical sections of water temperature when using the higher horizontal resolution. Furthermore, Karman vortex street was simulated in Ulleungdo-Dokdo 300 m model due to the terrain effect of th islands that was not shown in the Ulleungdo 1 km model.
Wheat (Triticum aestivum L.) is a major staple foods and is in increasing demand in the world. The elevated temperature caused by changes in climate and environmental conditions is a major factor affecting wheat development and grain quality. The optimal temperature range for winter wheat is between 15 and 25℃, and it is necessary to study the physiological characteristic of wheat according to elevated temperatures. This study presents the effect of elevated temperature on the yield and quality of two Korean bread wheat (Baekkang and Jokyoung) in temperature gradient tunnels (TGT). Two bread wheat cultivars were grown in TGT at four different temperature conditions: T0 (control, near ambient temperature), T1 (T0+1℃), T2 (T0+2℃), (T0+2℃), T3 (T0+3℃). The period from sowing to heading stage accelerated and the number of grains per spike and grain yield reduced under T3 condition compared with those under T0 condition. Grain filling rate and grain maturity also accelerated with elevated temperature (T3). The increase in temperature led to the increase in protein contents, whereas decreased the total starch contents. These results are consistent with the decreased expression of starch synthesis genes and increased gliadin synthesis or gluten metabolism genes during the late grain filling stage. Taken together, our results suggest that the increase in temperature (T3) led to the decrease in grain yield by regulating the number of grains/spike, whereas increased the protein content by regulating the expression of starch and gliadin-related genes or gluten metabolism process genes expression. In addition, our results provide a useful physiological information on the response of wheat to heat stress.
A delta is a depositional landform that is formed when sediment transported by a river is deposited in a relatively low-energy environment, such as a lake, sea, or a main channel. Among these, a delta formed at the confluence of rivers has a great importance in river management and research because it has a significant impact on the hydraulic and sedimentological characteristics of the river. Recently, the equilibrium state of the confluence area has been disrupted by large-scale dredging and construction of levees in the Nakdong River. However, due to the natural recovery of the river, the confluence area is returning to its pre-dredging natural state through ongoing sedimentation. The time-series data show that the confluence delta has been steadily growing since the dredging, but once it reaches a certain size, it repeats growth and retreat, and the overall size does not change significantly. In this study, we developed a model to explain the sedimentation-erosion processes in the confluence area based on the assumption that the confluence delta reaches a dynamic equilibrium. The model is based on two fundamental principles: sedimentation due to supply from the tributary and erosion due to the main channel. The erosion coefficient that represents the Nakdong River confluence areas, was obtained using data from the tributaries of the Nakdong River. Sensitivity analyses were conducted using the developed model to understand how the confluence delta responds to changes in the sediment and water discharges of the tributary and the main channel, respectively. We then used annual average discharge of the Nakdong River's tributaries to predict the dynamic equilibrium positions of the confluence deltas. Finally, we conducted a simulation experiment on the development of the Gamcheon-Nakdong River delta using recorded daily discharge. The results showed that even though it is a simple model, it accurately predicted the dynamic equilibrium positions of the confluence deltas in the Nakdong River, including the areas where the delta had not formed, and those where the delta had already formed and predicted the trend of the response of the Gamcheon-Nakdong River delta. However, the actual retreat in the Gamcheon-Nakdong River delta was not captured fully due to errors and limitations in the simplification process. The insights through this study provide basic information on the sediment supply of the Nakdong River through the confluence areas, which can be implemented as a basic model for river maintenance and management.
An investigation was conducted to determine the characteristics of seasonal occurrences and effective insecticides for minimizing the occurrence of Pseudaulacaspis cockerelli in sweet persimmon orchards. Most of the nymphs or female scales that mated in mid-September overwintered on twigs, matured to lay eggs in the next spring in accordance with temperature increase, and survived till mid to late May to lay all of their eggs. Males mostly died after mating by the end of September, while the males on the fallen leaves died during the hibernating period. Egg laying in P. cockerelli showed differences in accordance with the weather conditions in each year. They laid eggs from mid-April to mid- or late May, with a peak in early or mid-May. A female was estimated to lay 160 eggs; eggs hatched a week later from the peak period of egg laying. In summer, egg laying started in early or mid-July and ended in mid- or late August, with a peak in late July or early August; a female laid approximately 130 eggs in summer. The estimated egg period was 4 days. The first generation nymph that hatched from the eggs laid by the overwintered female on twigs showed 10% occurrence on twigs and 90% on leaves. The first generation nymphs on twigs mostly developed into female scales. The occurrence of the second generation nymphs reached a peak on July 27, 2009; they lived on the twigs as their overwintering site from mid- or late August. The female and male scales on leaves developed at the similar rate as the first generation nymphs up to August. The occurrence of male scales indicated that the peak occurrence of nymphs was on August 12, 2009, male scales on August 27, and adults approximately on September 14, which showed steady relationship in the sequence of development from nymphs to adult males. Among the nymphs that occurred on the leaves on August 12, 75% of them emerged into male adults, mated, and died. Buprofezine+dinotefurn (20+15) WP treatment on June 9 and 16 resulted in 90.6% control of P. cockerelli, when mortality was checked 7 weeks after treatment.
KIM, Do-Ryeong;KIM, Hyeong-Hun;KIM, Woo-Hyeon;RYU, Dong-Ha;GANG, Su-Myung;CHOUNG, Yun-Jae
Journal of the Korean Association of Geographic Information Studies
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v.19
no.4
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pp.63-75
/
2016
Aquaculture has historically delivered marine products because the country is surrounded by ocean on three sides. Surveys on production have been conducted recently to systematically manage aquaculture facilities. Based on survey results, pricing controls on marine products has been implemented to stabilize local fishery resources and to ensure minimum income for fishermen. Such surveys on aquaculture facilities depend on manual digitization of aerial photographs each year. These surveys that incorporate manual digitization using high-resolution aerial photographs can accurately evaluate aquaculture with the knowledge of experts, who are aware of each aquaculture facility's characteristics and deployment of those facilities. However, using aerial photographs has monetary and time limitations for monitoring aquaculture resources with different life cycles, and also requires a number of experts. Therefore, in this study, we investigated an automatic prototype system for detecting boundary information and monitoring aquaculture facilities based on satellite images. KOMPSAT-3 (13 Scene), a local high-resolution satellite provided the satellite imagery collected between October and April, a time period in which many aquaculture facilities were operating. The ANN classification method was used for automatic detecting such as cage, longline and buoy type. Furthermore, shape files were generated using a digitizing image processing method that incorporates polygon generation techniques. In this study, our newly developed prototype method detected aquaculture facilities at a rate of 93%. The suggested method overcomes the limits of existing monitoring method using aerial photographs, but also assists experts in detecting aquaculture facilities. Aquaculture facility detection systems must be developed in the future through application of image processing techniques and classification of aquaculture facilities. Such systems will assist in related decision-making through aquaculture facility monitoring.
This study was performed to develop a suitable nutrient solution for standard rose substrate culture in a closed hydroponic system. 1/4, 1/2, 2/3 and 1 strength of the nutrient solution made by Japan National Institute of Vegetable and Tea Science (JNIVT) were supplied. The photosynthesis rate, quality and growth of cut flower were higher in the 1/2 and 2/3 strength of nutrient solution during high and low temperature period. Based on the above results, optimum nutrient solutions (UOS) were composed by nutrientwater (n/w) absorption ratio with 1/2S ($NO_{3^-}N$ 6.8, $NH_{4^-}N$ 0.7, $PO_{4^-}P$ 2.0, K 3.8, Ca 3.0, Mg 1.2, $SO_{4^-}S$$1.2me{\cdot}L^{-1}$) at high temperature season and 2/3($NO_{3^-}N$ 9.7, $NH_{4^-}N$ 0.8, $PO_{4^-}P$ 2.2, K 5.0, Ca 3.9, Mg 1.5, $SO_{4^-}S$$1.5me{\cdot}L^{-1}$) at low temperature season. The results of suitability examination showed that the EC level in newly composed nutrient solution (UOS) was more stable than other nutrient solutions due to its large amount of calcium and potassium. The growth of cut flower cultivated with UOS was higher than those of other nutrient solutions. Especially, the yield of cut flowers in UOS nutrient solution increased 1.4 times than that of other nutrient solution treatments. Consequently, the new nutrient solution investigated in this experiment was suitable for rose cultivation in a closed hydroponic system.
For the clinical application, it is needed to keep characteristics of stem cells after storage for a long time. In the present study, we examined stem cell properties of human cord-derived stem cells (HUC) after cryopreservation. Cells were isolated from human umbilical cord and cultured in vitro. At passage 2 or 3, HUC were suspended at a concentration of $1.0{\times}10^6/m{\ell}$ in cryomedium consisting of DMSO and FBS. After freezing at $-80^{\circ}C$ overnight, HUC were cryopreserved at $-196^{\circ}C$ nitrogen gas. After 6 months, HUC were thawed and cultured in vitro. Assessment for the stem cell properties was made upon the morphology, population doubling time, and expression profiles of genes and various proteins. Cryopreserved HUC showed more than 70% viability and maintained fibroblast-like morphology similar to HUC before cryopreservation. Throughout the culture, they underwent average 42.8 doublings and produced $6.75{\times}{10^{18}}$ cells. RT-PCR analyses showed that cryopreserved HUC expressed Oct-4, nanog, SCF, NCAM, nestin, GATA-4, BMP4, and HLA-1 genes. They did not express Brachyury and HLA-DR genes. Immunocytochemical studies showed that cryopreserved HUC reacted with antibodies against SSEA-3, -4, Thy-1, vimentin, fibronectin, HCAM, ICAM, HLA-1 proteins. They did not react with antibody against HLA-DR protein. Theses genes and proteins expression patterns of cryopresserved HUC were similar to those of HUC before cryopreservation. These results suggest that cryopreserved HUC could retain proliferative potential and they expressed various genes and proteins similar to HUC before cryopreservation. Thus, cryopreservation might be useful for HUC for future research and clinical application.
Green tea is popular plants in Asian countries and has become more widespread in western countries due to its taste characteristics and health benefits. Apart from green tea leafs, however, the use of root and seed of green tea tree has not intensively been investigated yet. In this study, the contents of mineral, vitamin, total amino acid, free amino acid, and total polyphenol (catechin, caffeic acid, and chlorogenic acid) of the root and seed of green tea tree were analyzed for the development of healthy foods. For minerals, potassium contents were 1,052 and 1,480 mg/100g-dry weight of root and seed, respectively. The order of mineral contents were as follows: K > P > Ca > Mg > Na > Fe > Mn > Zn > Cu in root and K > P > Na > Ca > Mg > Mn > Fe > Cu > Zn in seed. For vitamins, vitamin C contents were 5.72 and 6.05 mg/100g-dry weight of root and seed, respectively. The presence of more various kinds of vitamins were observed in seed than in root. For total amino acids, the contents were 1,651 and 4,335 mg/100g-dry weight of root and seed, respectively. The total amino acid contents of seed and root were higher than those in commercial green tea products. Especially the phenylalanine contents were 16 and 139 mg/100g-dry weight of root and seed, respectively whereas phenylalanine was not found in commercial green tea products. Concerning free amino acids, the bitter tasting amino acids such as arginine, valine and tryptophan were more abundant in root and the sweet tasting ones such as glutamic acid, alanine, aspartic acid, and serine were more abundant in seed. The total polyphenol contents were 237 and 81 mg/100g-dry weight of root and seed, respectively. The polyphenol contents in root were three times higher than that in seed so root may be a better source for antioxidant ingredients than seed. Among many polyphenols, catechin, caffeic acid, and chlorogenic acid were the top three major components.
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