• International Journal of Industrial Entomology and Biomaterials
• /
• 제8권1호
• /
• pp.77-80
• /
• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• 한국산림과학회지
• /
• 제81권4호
• /
• pp.320-324
• /
• 1992

들메나무(Fraxinus mandshurica Rupr.) 는 우리나라에서 두가지의 서로 다른 형태(形態)가 자생(自生)하고 있는 것으로 알려져 있다. 최근(最近)에 개발(開發)된 PCR기법(技法)을 이용(利用)하여 이 두 형태(形態)의 들메나무 DNA의 변이(變異)를 조사(調査)하였다. DNA 합성(合成) 효소(酵素)와 인공합성(人工合成)된 primer를 이용(利用)하여 이 수종(樹種)의 DNA를 증폭(增幅)시켜 비교(比較)한 결과(結果)이 두 형태(形態)는 DNA 비례(排列)에서 서로 다른것으로 나타났다. DNA변이(變異)는 같은 형태내(形態內)의 개체간(個體間)에도 나타나나 각 형태별(形態別)로 뚜렷하게 구분(區分)되어 형태별(形態別)로 특징적(特徵的)인 band들이 관찰(觀察)되었다. 이러한 특징적(特徵的)인 band들로 두 형태(形態)를 구분(區分)할 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제16권2호
• /
• pp.75-86
• /
• 2008

In order to understand geographic genetic variation and relationship among populations of the mason bee (Osmia cornifrons Radoszkowsky), which is used as pollinator for apple tree, we sequenced a portion of mitochondrial (mt) COI gene, which corresponds to "DNA Barcode" region (658 bp) from 81 O. cornifrons individuals collected over eight localities in Korea. The sequence data revealed overall moderate to low genetic diversity within species, with a maximum sequence divergence of 0.76%. Geographically, two haplotypes (BAROC01 and BAROC02) were widespread with a frequency of 82.7%, whereas several haplotypes were found in a locality as a single individual, suggesting that haplotype distribution can be summarized as coexistence of a few widespread haplotypes and several regionally restricted haplotypes. Overall, high rate of per generation female migration (Nm=$1.1{\sim}$infinite) and low level of geographic subdivision ($F_{ST}=0{\sim}0.315$) among localities were characteristic. Although two populations (p < 0.026) were genetically subdivided from the remaining localities, no clear polarity was observed. Taken together, the nature of genetic divergence of the mason bee populations is characterized as one that possessing moderate to low genetic diversity, high gene flow, and wide spread haplotypes with ahigh frequency, concordant with the capability of dispersal in connection with the lack of historical biogeographic barriers.

• 방송공학회논문지
• /
• 제14권3호
• /
• pp.299-310
• /
• 2009

본 논문은 줌-렌즈로 취득한 비디오 영상에 대해서 줌-렌즈의 왜곡을 자동으로 보상할 수 있는 새로운 방법을 제안하였다. 먼저, 초점거리의 증가에 따라 렌즈의 왜곡 계수가 비선형적으로 단조 감소하는 특징으로부터 초점거리와 렌즈 왜곡 계수로 표현되는 비선형 줌-렌즈 왜곡 모델을 정의하였다. 그리고 취득한 비디오 영상으로부터 몇 장의 샘플 영상을 선정하고, 이 샘플영상에 대한 초점거리와 렌즈 왜곡 계수는 기존의 방법들을 이용하여 측정하였다. 이렇게 측정한 초점거리와 렌즈 왜곡 계수들로 부터 줌-렌즈 왜곡 모델을 최적화 시켰다. 최적화된 줌-렌즈 왜곡 모델은 각 비디오 영상의 초점거리를 입력으로 하여 렌즈 왜곡계수를 자동으로 계산할 수 있다. 본 논문에서 제안한 방법은 다양한 실사 영상과 비디오 영상에 적용하여 그 성능을 검증하였으며, 화질의 열화 없이 영상의 왜곡을 보상할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권5호
• /
• pp.768-774
• /
• 2007

To study the effect of dietary docosahexaenoic acid (DHA) enrichment on the expression of hepatic genes in pigs, weaned, crossbred pigs (30 d old) were fed diets supplemented with either 2% tallow or DHA oil for 18 d. Hepatic mRNA was extracted. Suppression subtractive hybridization was used to explore the hepatic genes that were specifically regulated by dietary DHA enrichment. After subtraction, we observed 288 cDNA fragments differentially expressed in livers from pigs fed either 2% DHA oil or 2% tallow for 18 d. After differential screening, 7 genes were found to be differentially expressed. Serum amyloid A protein 2 (SAA2) was further investigated because of its role in lipid metabolism. Northern analysis indicated that hepatic SAA2 was upregulated by dietary DHA enrichment (p<0.05). In a second experiment, feeding 10% DHA oil for 2d significantly increased the expression of SAA2 (compared to the 10% tallow group; p<0.05). The porcine SAA2 full length cDNA sequence was cloned and the sequence was compared to the human and mouse sequences. The homology of the SAA2 amino acid sequence between pig and human was 73% and between pig and mouse was 62%. There was a considerable difference in SAA2 sequences among these species. Of particular note was a deletion of 8 amino acids, in the pig compared to the human. This fragment is a specific characteristic for the SAA subtype that involved in acute inflammation reaction. Similar to human and mouse, porcine SAA2 was highly expressed in the liver of pigs. It was not detectable in the skeletal muscle, heart muscle, spleen, kidney, lung, and adipose tissue. These data suggest that SAA2 may be involved in mediation of the function of dietary DHA in the liver of the pig, however, the mechanism is not yet clear.

• 한국통신학회논문지
• /
• 제32권11A호
• /
• pp.1086-1095
• /
• 2007

본 논문에서는 다수의 피코넷들이 공존 (Simultaneously Operating Piconets; SOP) 하는 환경하에서 다중 상관처리를 이용한 새로운 Non-coherent TOA 추정 기법을 제안한다. SOP 환경에서 각 피코넷들을 구분하기 위해서 Gold 시퀀스를 이용한 DSSS (Direct Sequence Spread Spectrum) 기반의 IR-UWB (Impulse Radio-Ultra WideBand) 신호를 사용하였으며 상관 특성을 개선시키기 위해서 다중 마스트 처리 (Multiple Mask Operation; MMO) 방안을 제시하였다. 직접 LOS 경로에 대한 도착 시간 (Time Of Arrival; TOA) 정보는 2단계의 대략적인(Coarse)/ 정밀 (Fine) 타이밍 검출 과정을 통해 획득된다. 제안된 기법에 대한 성능을 검증하기 위해서 IEEE 802.15.4a TG (Task Group)에서 제시한 두 채널 모델을 적용하였고 시뮬레이션 결과로부터 제안된 기법이 다수개의 피코넷이 공존하는 다중 경로 환경에서 일반적인 단일 상관 처리 기법보다 성능이 개선됨을 확인하였다.

• 한국발생생물학회지:발생과생식
• /
• 제18권4호
• /
• pp.275-286
• /
• 2014

To successful molecular breeding, identification and functional characterization of breeding related genes and development of molecular breeding techniques using DNA markers are essential. Although the development of a useful marker is difficult in the aspect of time, cost and effort, many markers are being developed to be used in molecular breeding and developed markers have been used in many fields. Single nucleotide polymorphisms (SNPs) markers were widely used for genomic research and breeding, but has hardly been validated for screening functional genes in olive flounder. We identified single nucleotide polymorphisms (SNPs) from expressed sequence tag (EST) database in olive flounder; out of a total 4,327 ESTs, 693 contigs and 514 SNPs were detected in total EST, and these substitutions include 297 transitions and 217 transversions. As a result, 144 SNP markers were developed on the basis of 514 SNP to selection of useful gene region, and then applied to each of eight wild and culture olive flounder (total 16 samples). In our experimental result, only 32 markers had detected polymorphism in sample, also identified 21 transitions and 11 transversions, whereas indel was not detected in polymorphic SNPs. Heterozygosity of wild and cultured olive flounder using the 32 SNP markers is 0.34 and 0.29, respectively. In conclusion, we identified SNP and polymorphism in olive flounder using newly designed marker, it supports that developed markers are suitable for SNP detection and diversity analysis in olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제9권2호
• /
• pp.187-191
• /
• 2004

Actin is a ubiquitous and highly conserved protein found in eukaryotic organisms. In this study, we describe the cDNA cloning and mRNA expression of an actin gene from the mulberry longicorn beetle, Apriona germari. The A. germari actin cDNA is 1524 bp containing a complete 1128 bp open reading frame that encodes a polypeptide of 376 amino acid residues with a predicted molecular weight of about 41.5 kDa. The deduced amino acid sequence of the A.germari actin cDNA showed 99% protein sequence identity to Homalodisca coagulata actin, differing at only two amino acid positions, and 92-98% protein sequence identity to known insect species actins. The predicted three-dimensional structure of A. germari actin revealed the four residue hydrophobic pulg loop characteristic of the actin family. Northern blot analysis showed that A. germari actin is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body of A. germari larva.

• Journal of Yeungnam Medical Science
• /
• 제36권3호
• /
• pp.231-240
• /
• 2019

Background: We sought to determine the value of combining diffusion-weighted (DW) and perfusion-weighted (PW) sequences with a conventional magnetic resonance (MR) sequence to assess solid components of borderline ovarian tumors (BOTs) and stage I carcinomas. Methods: Conventional, DW, and PW sequences in the tumor imaging studies of 70 patients (BOTs, n=38; stage I carcinomas, n=32) who underwent surgery with pathologic correlation were assessed. Two independent radiologists calculated the parameters apparent diffusion coefficient (ADC), $K^{trans}$ (vessel permeability), and $V_e$ (cell density) for the solid components. The distribution on conventional MR sequence and mean, standard deviation, and 95% confidence interval of each DW and PW parameter were calculated. The inter-observer agreement among the two radiologists was assessed. Area under the receiver operating characteristic curve (AUC) and multivariate logistic regression were performed to compare the effectiveness of DW and PW sequences for average values and to characterize the diagnostic performance of combined DW and PW sequences. Results: There were excellent agreements for DW and PW parameters between radiologists. The distributions of ADC, $K^{trans}$, and $V_e$ values were significantly different between BOTs and stage I carcinomas, yielding AUCs of 0.58 and 0.68, 0.78 and 0.82, and 0.70 and 0.72, respectively, with ADC yielding the lowest diagnostic performance. The AUCs of the DW, PW, and combined PW and DW sequences were $0.71{\pm}0.05$, $0.80{\pm}0.05$, and $0.85{\pm}0.05$, respectively. Conclusion: Combining PW and DW sequences to a conventional sequence potentially improves the diagnostic accuracy in the differentiation of BOTs and stage I carcinomas.

• 한국음향학회지
• /
• 제41권1호
• /
• pp.16-29
• /
• 2022

본 논문에서는 수중통신 장치가 수중에서 이동시 발생하는 도플러(Doppler) 편이를 극복하기 위한 도플러 편이 추정방법과 직접수열 확산대역 전송의 신호처리 방법을 제시한다. 제안한 방법은 도플러 편이에 강인하고, 자기 상관 특성이 우수한 64길이의 Frank 신호열을 두 번 반복하는 프리앰블을 사용하여 2단계로 도플러 편이를 추정한다. 또한, 제안한 방법을 직접수열 대역확산 전송을 사용하는 수중통신방식에 적용하여 수중이동통신용 패킷 설계와 RAKE 수신기를 설계한다. 설계한 확산대역 수중이동통신 변조방식은 데이터 심볼을 Differential-Quadrature Phase Shift Keying(DQPSK) 변조하여 수신시 위상추적이 필요 없고, RAKE 수신기에서 동위상 결합이 용이하게 할 수 있는 장점이 있다. 제안한 도플러 편이 추정방법을 포함하는 RAKE 수신방법은 약 17.5 m/s의 이동속도까지 가상으로 이동하는 송신기로부터 수신한 직접수열 대역 확산 신호를 성공적으로 수신한다.