• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1987년도 Proceedings of Korea-Japan Panax Ginseng Symposium 1987 Seoul Korea
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• pp.47-58
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• 1987

Ethanol exerts different effects on hepatic cellular metabolism, depending mainly on the duration of its intake. In the presence of ethanol following an acute load, a number of hepatic functions are inhibited, including lipid oxidation and microsomal drug metabolism. In its early stages, chronic ethanol consumption produces adaptive metabolic changes in the endoplasmic reticulum which result in increased metabolism of ethanol and drugs and accelerated lipoprotein production. Prolongation of ethanol intake may result in injurious hepatic lesions such as alcoholic hepatitis and cirrhosis A number of such metabolic effects of ethanol are directly linked to the two major products of its oxidation; hydrogen and acetaldehyde. The excess hydrogen from ethanol unbalances the liver cell's chemistry. In the presence of excess hydrogen ions the process is turned in a different direction. In this study, it was attempted to observe the effect of ginseng saponins on alcohol Oehydrogenase(ADH), aldehyde dehydrogenase(ALDH) and microsomal ethanol oxidizing system(MEOS) in vivo as well as in vitro. Furthermore, the effect of ginseng saponin on the hydrogen balance in the liver and the hepatic cellular distribution of (1-14C) ethanol, its incorporation into acetaldehyde and lipids was also investigated. It seemed that ginseng saponin stimulated the above enzymes and other related enzymes in ethanol metabolism, resulting in a rapid removal of acetaldehyde and excess hydrogen from the animal body,

• 동의생리병리학회지
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• 제20권6호
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• pp.1678-1727
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• 2006

This study was perfromed to develop the assessment guideline and endpoints for clinical trial with anticancer herbal medicine. The botanical products used to humans for long time may be applied to phase 3 clinical trial after submitting the evidences for safety and efficacy of them or completion of basic requirement of phase 1 and phase 2 for safety confirmation and dose determination. Syndrome improvement was chiefly evaluated by Zubrod and karnofsky(%) methods. We suggest the general clinical trial assessment with botanical products, by following assessment points, that is, tumor size for 50 points, survival fate for 10 points, major syndromes for 40 points. It is recommendable that the each symptom of Qi deficiency syndrome, blood deficiency syndrome and Qi stagnation syndrome was allocated by assessment points, Similarly, the each symptom was given the assessment points according to the severity of symptom, for example, slight for 3 points, moderate for 2 points and severe for 1 point in hepatocelluar carcinoma and lung cancer. Then, the efficacy of botanical products was evaluated by the difference between pre-treatment and post-treatment. Asking the neoplastic patients of questionnaire on physical, emotional, cognitive, social and role subjects availability, three more syndromes (Fatigue, Pain and Nausea/Vomit), quality of life(QOL) will be evaluated by GLM statistics. In addition, in case of lung cancer, 13 questions will be asked by the EORTC QLQ-C13 forms. As the assessment of endpoints for efficacy to reduce side effects induced by chemotherapy and radiotherapy, the data of image scanning and hemato-urinalysis can be usefully applied on immune response, weight loss, indigestion, hemopoietic damage and injury of liver and kidney, while the changes of syndromes of side effect can be evaluated by differentiation methods of Qi and blood and five viscera. However, it is still necessary to determine the ratio between scientific analytical method and Oriental differentiation method as well as confirm the Oriental assessment endpoints by clinical trial. In addition, we suggest the continuous development of assessment endpoints on other carcinomas except of hepatocelluar carcinoma and lung cancer in future.

• 대한한의학회지
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• 제20권3호
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• pp.9-17
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• 1999

Objectives: Recently a renovation of the medical-welfare system to reflect the changes of disease spectrum with the demographic changes of society, the increase in income level, and marked concerns for health promotion has been demanded. In accordance with this, attempts have been made to actively integrate traditional medicine based on symptom-differentiated treatment and Western medicine based on disease treatment so that they can complement each other. China has already tried a complementary medical treatment system integrating traditional Chinese and Western medicine. So, this article reviewed major advances in research on integrated traditional Chinese medicine and Western medicine in China. Methods: The authors analyzed data from clinical articles and experimental works in the ' Chinese Journal of Integrated Traditional and Western Medicine' Results and conclusions: Each department attempted to integrate Traditional Chinese Medicine(TCM) and Western Medicine in treatment of various diseases such as malaria, AIDS, and intoxication (rarely found in Korea clinically). Especially in the departments of surgery, dentistry, radiology, and anesthesiology we could see the frequent use of combined treatment. TCM and Western medicine complemented each other very successfully, and the effect of the combined therapy was superior to that of traditional therapy alone. There were diverse methods for therapy in integrated TCM and Western medicine; bath-Tx, physical-Tx, manipulative-Tx, drug -acupuncture, Tibetan medicine, etc. were available in therapy as well as traditional methods such as acupuncture, moxibustion, and negative- Tx. The way of producing Chinese medications were diversified and formulated; making new prescriptions, compounding various kinds of new medicine called' Zhong Cheng Yao' (中成藥) which were easily made, stored, and taken. 'Diagnosis Criteria', 'The effect of TCM Treatment Criteria' were made by committee and broadly used for objectifying diagnosis, discriminating effects of treatments and treatment development, and developing new medical products.

• Macromolecular Research
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• 제12권1호
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• pp.46-52
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• 2004

In a continuation of our previous studies on blood compatibility profiles of anion-substituted poly(vinyl alcohol) (PVA) membranes, in which hydroxyl groups have been replaced with carboxymethyl (C-PVA) and sulfonyl groups (S-PVA), we have studied the activation of complement components and the changes in white cell and platelet count in vitro and compared them with those of unmodified PVA, Cuprophane, and low-density polyethylene. Complement activation of fluid phase components, C3a, Bb, iC3b, and SC5b-9, and of bound phases, C3c, C3d, and SC5b-9, were assessed by enzyme-linked immunosorbent assay (ELISA) and immunoblot, respectively. The changes in the number of white cells and platelets following complement activation were counted using a Coulter counter. C-PVA and S-PVA activated C3 to a lesser extent than did PVA, which we attribute to the diminished level of surface nucleophiles of the samples. In addition, C- and S-PVA exhibit increased inhibition of Bb production, resulting in a decrease in the extent of C5 activation. Consequently, because of the reduced activation of C3 and C5, C- and S-PVA samples cause marked decreases in the SC5b-9 levels in plasma. We also found that the negatively charged sulfonate and carboxylate groups of the samples cause a greater extent of adsorbtion of the positively charged anaphylatoxins, C3a and C5a, because of strong electrostatic attraction, which in turn provides an inhibition of chemotaxis and activation of leukocytes. The ability to inhibit complement production, together with the binding ability of anaphylatoxins of the C- and S-PVA samples, leads to a prominent decrease in lysis of leukocytes as well as activation of platelets.

• 한국식품영양과학회지
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• 제46권4호
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• pp.501-512
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• 2017

어린이 식생활안전관리 특별법에 근거한 고열량 저영양 식품의 관리 정책이 본격적으로 시행된 이후, 2010년 3월 말과 2012년 7월 말 기준 생산 판매가 지속된 어린이 기호식품, 단종 어린이 기호식품과 신규 어린이 기호식품을 대상으로 단위용량당 평균 열량 및 영양성분(포화지방, 단백질, 당류, 나트륨)의 개선 여부를 분석한 주요 결과는 다음과 같다. 첫째, 2010년 3월 말과 2012년 7월 말 기준 생산 판매가 지속된 어린이 기호식품은 총 6,174개, 이 중 고열량 저영양 식품은 1,249개(20.2%)였다. 또한, 단종 어린이 기호식품은 총 510개, 이 중 고열량 저영양 식품은 206개(40.4%)였으며, 신규 어린이 기호식품은 총 1,173개, 이 중 고열량 저영양 식품은 총 371개(31.6%)로 조사되었다. 둘째, 생산 판매가 지속된 어린이 기호식품 중 평균 열량 또는 영양성분에서 긍정적인 변화만이 1개 항목 이상 나타난 식품유형은 빵류, 아이스크림류, 과채음료, 즉석섭취식품(이상 1개 항목), 혼합음료(2개 항목), 빙과류(3개 항목)였다. 반면 부정적인 변화만이 1개 항목 이상에서 나타난 식품유형은 과자, 초콜릿류, 유산균음료(이상 1개 항목), 캔디류(2개 항목)였다. 한편 어육소시지, 탄산음료, 유탕면류 및 국수, 피자에서는 긍정적인 변화와 부정적인 변화가 동시에 나타났다. 다만 단위용량당 평균 열량 또는 영양성분의 변화는 대부분 1 kcal 또는 1 g 미만이었다. 셋째, 열량 및 영양성분 함량의 긍정적인 변화는 어린이 기호식품 중에서도 고열량 저영양 식품에서 더욱 뚜렷하게 나타났다. 생산 판매가 지속된 고열량 저영양 식품 중 평균 열량 또는 영양성분에서 긍정적인 변화만이 1개 항목 이상 나타난 식품유형은 과채음료, 유산균음료, 혼합음료(이상 1개 항목), 빙과류(3개 항목), 아이스크림류(3개 항목), 즉석섭취식품(3개 항목)이었다. 반면 부정적인 변화만이 1개 항목 이상 나타난 식품유형은 캔디류, 초콜릿류, 탄산음료(이상 1개 항목)였다. 한편 빵류, 유탕면류 및 국수, 피자의 평균 열량 또는 영양성분에서는 긍정적인 변화와 부정적인 변화가 동시에 관찰되었다. 단위 용량당 평균 열량 또는 영양성분의 변화는 대부분 1 kcal 또는 1 g 미만이었으나, 일부 식품유형(빙과류, 빵류, 즉석 섭취식품, 피자)에서는 상대적으로 큰 변화가 발견되기도 하였다. 마지막으로 신규 어린이 기호식품이 단종 어린이 기호식품보다 평균 열량 또는 영양성분에서 긍정적인 변화만이 1개 항목 이상 나타난 식품유형은 빵류(3개 항목)와 탄산음료(2개 항목)였으며, 부정적인 변화만이 1개 항목 이상 나타난 식품유형은 초콜릿류(4개 항목), 아이스크림류(4개 항목), 즉석섭취식품(3개 항목)이었다. 과자, 캔디류, 과채음료, 혼합음료, 유탕면류 및 국수, 피자에서는 긍정적인 변화와 부정적인 변화가 동시에 관찰되었다. 본 연구 결과 고열량 저영양 식품 관리 정책이 본격적으로 실시된 이후에도 어린이 기호식품의 단위용량당 평균 열량 및 영양성분상에 일부 부정적인 변화를 확인할 수 있었다. 이는 식품회사들이 자율적으로 제품의 긍정적인 개선을 적극 실천하기보다는, 현행 고열량 저영양 식품 분류 기준에 저촉되지 않는 선에서라면 일부 부정적인 방향으로의 제품 변화 역시 시도하고 있음을 시사한다. 따라서 고열량 저영양 식품 관리 정책이 어린이 기호식품의 영양개선을 통하여 어린이를 위한 건강한 식생활 환경 조성에 미친 영향은 아직까지 일부 식품 유형에만 제한적이라 할 수 있다.

• Journal of Acupuncture Research
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• 제20권5호
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• pp.93-106
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• 2003

Objective : To investigate the possible molecular mechanism(s) of melittin as a candidate of anti-cancer drug, we examined the effects of the compound on the growth of human lung carcinoma cell line A549. Methods: MTT, morphological changes, DAPI staining, Western blot, RT-PCR and in vitro prostaglandin E2 (PGE2) accumulation assays were performed. Results: The anti-proliferative effect by melittin treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. Melittin induced apoptotic cell death in a concentration-dependent manner, which was associated with inhibition or degradation of apoptotic target proteins such as ${\beta}$-catenin, poly(ADP-ribose) polymerase(PARP) and phospholipase $C-{\gamma}1(PLC-{\gamma}1)$. Melittin treatment inhibited the expression of cyclooxygenase-2(COX-2) and accumulation of PGE2 in aconcentration-dependent fashion. In addition, Melittin treatment induced the down-regulation of telomerase reverse transcriptase(hTERT) and proto-oncogene c-myc expression of A549 cells. Conclusions: Taken together, these findings suggest that melittin-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products, and melittin may have therapeutic potential in human lung cancer.

• 생물정신의학
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• 제7권2호
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• pp.115-122
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• 2000

The development of molecular biology has brought many changes in psychiatry. Molecular biology makes us possible to know the cause of mental disorders that provide the way to prevent the disorders, and to develop various accurate diagnostic and treatment methods for mental disorders. The author discusses the concept, cause, and treatment of mental disorders in the aspect of molecular biology. Importing the methods of molecular biology into psychiatry, we can anticipate to get a number of the goals of psychiatric genetics, including identification of specific susceptibility genes, clarification of the pathophysiological processes whereby these genes lead to symptoms, establishment of epigenetic factors that interact with these genes to produce disease, validation of nosological boundaries that more closely reflect the actions of these genes, and development of effective preventive and therapeutic interventions based on genetic counseling, gene therapy, and modification of permissive or protective environmental influences. In addition to their capacity to accelerate the discovery of new molecules participating in the nervous system's response to disease or to self-administered drugs, molecular biological strategies can also be used to determine how critical a particular gene product may be in mediating a cellular event with behavioral importance. Molecular biology probably enables us discover the environmental factors of mental disorders and allow rational drug design and gene therapies for mental disorders, by isolation of gene products that facilitate a basic understanding of the pathogenesis of these disorders. A specific genetic linkage may suggest a novel class of drugs that has not yet been tried. With respect to gene therapy, the hypothetical method would use a gene delivery system, most likely a modified virus, to insert a functional copy of a mutant gene into those brain cells that require the gene for normal function.

• 한국가금학회지
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• 제43권4호
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• pp.235-242
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• 2016

본 연구는 식품의약품안전처로부터 지난 5년간 가금육(닭고기, 오리고기) 중 일반세균수 모니터링 검사 결과를 받아 분석하고, 현재 서울/경기, 충청도 및 강원도 지역 내 유통단계(식육포장처리장, 식육판매장)의 닭고기를 대상으로 미생물학적 오염도를 조사하였다. 또한, 닭가슴살의 저장기간의 따른 이화학적, 미생물학적, 관능적 특성의 변화를 분석을 통하여 적정 미생물 권장 기준에 대한 과학적인 근거를 제시하고자 하였다. 현재 국외에서는 유통단계 식육의 일반세균수에 대하여 $1{\times}10^5{\sim}10^6CFU/g$ 또는 $CFU/cm^2$ 이하로 권장 또는 규제하고 있다. 반면에, 우리나라의 권장기준치는 $1{\times}10^7CFU/g$ 이하로 국외와 비교하였을 때 완화된 권장기준치를 가지고 있다. 실제 지난 5년간 시행된 전국의 유통단계 가금육(닭고기, 오리고기) 중 일반세균수 모니터링하여 분석해본 결과, 대부분 $1{\times}10^6CFU/g$ 이하에 속하며, 권장기준 초과 건수가 1% 이내로 위생관리가 잘 이루어지고 있었다. 이를 바탕으로 현재 서울/경기, 강원도 및 충청도 지역의 유통단계 닭고기의 현장 모니터링을 실시하여 18개의 식육 판매점에서 채취된 54개의 시료 중 2건을 제외하고, 모두 국내 일반세균수 권장기준치인 $1{\times}10^7CFU/g$과 대장균수 권장 기준치인 $1{\times}10^4CFU/g$을 초과하지 않았다. 적정 미생물 권장기준에 과학적 근거 제시를 위하여 유통기간 중에 발생할 수 있는 품질적 변화를 예측하고자 닭가슴살을 저밀도 랩으로 포장하여 $4^{\circ}C$에서 저장하며, 저장기간별 이화학적, 미생물학적 및 관능학적 변화를 분석하였다. 그 결과, 현재 국내 유통단계 닭고기 중 일반세균수 권장기준치인 $1{\times}10^7CFU/g$에 근접하였을 때 pH는 5.96으로 pH 6에 가까웠고, 종합적 기호도가 5.11점으로 5점에 가까웠으며 특히 냄새가 5점 이하를 보여 이취로 인한 선호도의 저하를 나타냈다. 따라서 국내 유통 단계 식육에 대한 일반세균수 권장기준치가 국외에 비해 다소 완화된 기준이며, 심지어 권장기준치($1{\times}10^7CFU/g$ 이하) 이하에서도 관능패널들이 낮은 선호도를 보였던 점을 고려할 때, 안전성뿐만 아니라 소비자의 기호도를 동시에 만족시킬 수 있도록 유통단계 닭고기 중 일반세균수 권장기준을 $1{\times}10^6CFU/g$ 수준까지 낮추어 보다 현실적이고 적정한 권장기준에 대한 검토가 필요할 것으로 판단된다.

• International journal of advanced smart convergence
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• 제10권1호
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• pp.197-208
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• 2021

The deer antler has been used as a major drug in oriental medicine for a long time. Recently, the demand for easy-to-take health functional foods is increasing due to economic development and changes in diet. As part of research on the development of functional materials for antlers, lactic acid fermentation of antler extract was performed. It was intended to develop a functional material with enhanced total polyphenol and flavonoid content and enhanced antioxidant activity. Lactic acid bacteria fermentation was performed by adding 4 types of lactic acid bacteria starter products, B. longum, Lb. Plantarum, Lb. acidophilus and mixture of 8 types of lactic acid bacteria to the antler water extract substrate, respectively. During the fermentation of lactic acid bacteria, the number of proliferation, total polyphenol and total flavonoid content, DPPH radical scavenging and antioxidant activity were quantified and evaluated. As a result of adding these four types of lactic acid bacteria to the antler water extract substrate, the number of lactic acid bacteria measured was 2.04~5.00×107. Meanwhile, a protease (Baciullus amyloliquefaciens culture: Maxazyme NNP DS) was added to the antler extract to decompose the peptide bonds of the contained proteins. Then, these four types of lactic acid bacteria were added and the number of lactic acid bacteria increased to 2.84×107 ~ 2.21×108 as the result of culture. The total polyphenol contents were 4.82~6.26 ㎍/mL in the lactic acid bacteria fermentation extracts, and after the reaction of protease enzyme and lactic fermentation, increased to 14.27~20.58 ㎍/mL. The total flavonoid contents were 1.52~2.21 ㎍/ml in the lactic acid bacteria fermentation extracts, and after the protease reaction and fermentation, increased to 5.59 ~ 8.11 mg/mL. DPPH radical scavenging activities of lactic acid bacteria fermentation extracts was 17.03~22.75%, but after the protease reaction and fermentation, remarkably increased to 32.82~42.90%.

• 대한영양사협회학술지
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• 제30권3호
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• pp.161-180
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• 2024

Social and environmental changes, such as the rise of single-person households and advances in the food industry, have led to the replacement of home-cooked meals with home meal replacements products (HMRs). This study compared the nutrient content of a total of 1,680 HMRs and 158 restaurant foods by collecting data on the nutrient content of comparable food types from the Food composition data for restaurant foods published by the Ministry of Food and Drug Safety (MFDS) and evaluating the calorie and nutrient content of HMRs based on nutrition labeling through market research from May 2022 to May 2024, focusing on rice, porridge, noodles, soup/stew. The nutritional content and price of the HMRs varied widely, even for similar foods, depending on the detailed food type. Therefore, it is necessary to make an appropriate choice based on nutrition labeling according to the purpose of consumption. The HMRs had a lower calorie and nutrient content due to the smaller serving size when compared with restaurant foods. However, when the same weights were compared, the sugar and sodium content in the rice and soup/stew were higher in the HMRs than in the restaurant foods. In addition, due to the wide variety of HMRs available, many HMRs that can replace restaurant foods are being produced. However, even for the same type of food, the serving sizes of the HMRs and the restaurant foods were widely different, suggesting the need for a study to examine the appropriateness of the serving sizes of HMRs and restaurant foods.