• 한국발생생물학회지:발생과생식
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• 제15권3호
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• pp.223-230
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• 2011

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants derived from incomplete combustion of carbons and crude oil. In this study, we investigated the effects of benzo[a]pyrene (B[a]P), a representative PAHs on in vitro sex steroid hormone production and germinal vesicle breakdown (GVBD) using isolated oocytes of longchin goby (Chasmichthys dolichognathus) and chameleon goby (Tridentiger trigonocephalus). Oocytes in diameters of 0.8-0.9 (end vitellogenic stage) and 0.9-1.0 mm (germinal vesicle migratory stage) from longchin goby and 0.5 mm (fully vitellogenic stage) from chameleon goby were used. In GVBD assay, B[a]P at 10 nM stimulated GVBD in the oocytes of 0.8-0.9 mm from longchin goby. B[a]P at 1 nM stimulated GVBD in the oocytes with diameter 0.5 mm from chameleon goby. In steroid production from oocytes of longchin goby, B[a]P at 100 nM decreased testosterone (T) production, B[a]P at 1,000 nM increased estraiol-17 (J (E2) production and 10 and 100 nM increased $17,20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) production in the oocytes with diameter 0.8-0.9 mm. B[a]P at 1,000 nM increased E2 production, 100 and 1,000 nM increased $17{\alpha}20{\beta}P$ production in the oocytes with diameter 0.9-1.0 mm. In steroid production of oocytes from chameleon goby, B[a]P at 1,000 nM increased $E_2$ production. B[a]P at 10 nM increased $17{\alpha}20{\beta}P$ production. In the ratio of $E_2$ to T ($E_2$/T), B[a]P at 100 and 1,000 nM increased $E_2$/T in the oocytes of longchin goby. B[a]P at 100 nM also increased $E_2$/T in the oocytes of chameleon goby. Taken together, these results suggest that B[a]P have not only weak estrogenic effects but progestogenic effects on oocyte maturation.

• 한국수산과학회지
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• 제51권1호
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• pp.54-63
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• 2018

We investigated the effect of $CO_2$ exposure on the reproductive process of chameleon goby Tridentiger trigonocephalus. Rearing aquaria were exposed for 90 days to $CO_2$ gas through diffuser connected with pH controller maintaining at pH 7.2 ($156.31{\pm}7.90ppm$) in low treatment, and at pH 6.5 ($274.17{\pm}6.51ppm$) in high treatment. $CO_2$ treatment had no significant effects on survival rates although the value was decreased compared to the controls. In female fish, $CO_2$ treatment had no significant effects on gonadosomatic index (GSI), hepatosomatic index (HSI) and condition factor (CF). However, high $CO_2$ treatment decreased HSI and CF in males significantly compared to the controls (P<0.05). The spawning occurrence was 6 times in the low level $CO_2$ treatment, and 4 times in the high level $CO_2$ treatment although only once in the controls. For the histological observations, there was no significant difference in $CO_2$ treatments. However, in male fish, $CO_2$ treatment delayed the formation of sperm from spermatid compared to controls. These results suggest $CO_2$ may disrupt reproductive process by delaying gametogenesis in chameleon goby and it was more sensitive in males.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.353-361
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• 2013

The objective of this study was to characterize the reproductive cycle of the chameleon goby, T. trigonocephalus. Gonadal development was investigated using a histological method. Specimens were collected monthly, from April 2009 to March 2010. The gonadosomatic index (GSI) of females began to increase in April, reaching the maximum in May, and declined sharply in August. In males, the GSI began to increase in April and reaching the maximum in July. The annual reproductive cycle of T. trigonocephalus can be divided into four successive stages in females: the growing (November-March), maturing (April-May), ripe and spawning (June-July), and recovery (August-October) stages. Males passed through growing (November-March), maturing (April-June), ripe and spermiation (July-August), and recovery (September-October) stages. These results indicate the spawning season is from June to July. The relationship between fecundity (Fc) and body length (BL) was $Fc=86.1511BL^{2.6506}$. Fecundity was ranged from 3,448-9,654 eggs in a BL of 4.8-7.2 cm and it was increased as BL increased.

• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.51-56
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• 2014

This study describes the developmental process of gonads in chameleon goby, Tridentiger trigonocephalus from the stage of hatching to 100 days after hatching (DAH). Based on histological observation, the primordial germ cells were observed in mesentery between mesonephric duct and gut at 15 DAH (total length, TL: $6.8{\pm}0.2mm$). At 20 DAH (TL: $7.9{\pm}0.1mm$), the primordial gonad began to protrude into peritoneal cavity and developed between mesonephric duct and gut. Initial ovarian differentiation was identified by the presence of ovarian cavity and oogonia in the gonads at 55 DAH (TL: $21.1{\pm}1.3mm$). Testicular differentiation started at 65 DAH (TL: $23.7{\pm}0.9mm$) with appearance of spermatogonial cells in the gonads. These findings indicate that sex differentiation in T. trigonocephalus occurs earlier in females than males, suggesting that this species can be classified as an undifferentiated gonochorist.

• 한국어류학회지
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• 제30권3호
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• pp.137-143
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• 2018

이 연구의 목적은 우리나라 연안에 서식하는 두줄망둑의 자치어 골격발달을 관찰하여 분류학적 연구자료로 이용하는 것이다. 부화 후 7일째 평균전장 $4.44{\pm}0.28mm$ (n=5) 일 때 섭이기능을 하는 윗턱에는 전상악골, 주상악골이 골화하였고, 아래턱에는 악골이 골화하기 시작하였다. 부화 후 13일째 평균전장 $5.62{\pm}0.24mm$ (n=5) 일 때 두개골의 액골, 노정골, 상이골이 골화하였다. 부화 후 32일째 평균전장 $11.8{\pm}0.28mm$ (n=5) 일 때는 견대부에 사출골, 후쇄골이 골화하였고, 부화 후 40일째 평균전장 $13.3{\pm}0.21mm$ (n=5) 일 때 두개골의 측사골이 골화하면서 모든 골격의 골화가 완료되었다.

• 한국해양학회지:바다
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• 제8권3호
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• pp.307-316
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• 2003

천해 내만 해역인 남해 서부의 도암만 어류군집 조성을 조사하기 위하여 2001년 3월부터 2002년 2월까지 조망어구(beam trawl)를 이용하여 매월 중순경 최간조시에 어류를 채집하였다. 조사기간동안 총 33과 53종의 어류가 채집되었다. 도화망둑(Chaeturichthys hexanema), 주둥치(Leiognathus muchalis), 문절망둑(Acanthogobius flavimanus)의 3종이 전체 채질개체수의 67.4％를 차지하였으며, 생체량에서는 꼼치(Liparis tanakai), 문절망둑(Acanthogobius flavimanus), 도화망둑(Chaeturichthys hexanema)이 50.8％를 차지하는 우점종이었다. 채집 개체수와 생체량은 가을과 겨울에는 상대적으로 높게 나타났던 반면, 출현종수와 다양도 등의 일변량 지수값들은 대체로 봄과 여름에 높았다. 주요 14종의 월별 출현빈도에 의한 종간 집괴분석 결과 이 해역의 어류군집이 계절별로 출현양상을 달리하는 3개의 무리로 구분될 수 있음을 보였다: 1)계절에 관계없이 연중 출현하는 주거종에 속하는 도화망둑, 문절망둑, 개서대(Cynoglossus robustus) 및 반지(Setipinna taty)와 가을에 높은 출현 빈도를 보인 비늘양태(Onigocia spinosa), 돛양태 (Repomucenus lunatus), 주둥치, 2) 여름에 높은 출현빈도를 보인 노랑촉수(Upeneus japonicus), 갈치 (Trichiurus lepturus) 및 실망둑(Cryptocentrus filifer), 3) 겨울과 봄에 주로 출현하는 종으로 꼼치(Liparis tanakai), 풀미역치(Erisphex pottii), 두줄망둑(Tridentiger trigonocephalus) 및 동갈양태 (Repomucenus richardsottii). 어류군집의 계절에 따른 뚜렷한 분리는 나타나지 않았는데, 이와 같은 결과는 회유종들의 계절에 따른 일시 출현이 나타난다 하더라도, 전체 어류군집 조성이 출현 개체수에서 높은 점유율을 보이는 주거종에 의해 크게 지배를 받아 비교적 안정된 군집을 형성하고 있음을 시사하였다.