• Journal of Acupuncture Research
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• v.15 no.2
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• pp.29-41
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• 1998

The effect of Cervi Cornu Parvum aqua-acupuncture on serum levels of sex hormone was investigated in ovariectomized rats. Water extract of Cervi Cornu Parvum was daily injected for 30 days at points in rats corresponding to bilateral Taixi (Ki. 3) points of human and non-point at root of tail after ovariectomy, respectively. Serum levels of estradiol, testosterone, progesterone, LH and FSH were measured at 2 and 4 weeks after aqua-acupuncture treatment, respectively. Compared with ovariectomized rats, significant increase of estradiol and progesterone and significant decrease of FSH and LH were elicited at 2 and 4 weeks after aqua-acupuncture treatment. Aqua-acupuncture treatment at non-point did not produce any remarkable effect. These findings suggest aqua-acupuncture may restore dysfunction of hypothalamic-pituitary-ovarian axis, associated with a significant increase of serum estradiol level in ovariectomized rats.

• Journal of Korean Medicine Rehabilitation
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• v.20 no.1
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• pp.61-77
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• 2010

Objectives : The purpose of this study was to investigate the effects of Bee Venom (BV) and Cervi Cornu Parvum pharmacoacupuncture (CC) in monosodium iodide induced arthritis rats. Methods : The subjects were divided into 5 groups ; Normal, Control (no treatment after MIA), BV (Bee Venom pharmacoacupuncture $100{\mu}{\ell}$ daily at Dokbi (ST35) after inducing MIA), CC (Cervi Cornu Parvum pharmacoacupuncture $100{\mu}{\ell}$ dailyat Dokbi (ST35) after inducing MIA) and BV+CC (Bee Venom pharmacoacupuncture and Cervi Cornu Parvum pharmacoacupuncture $100{\mu}{\ell}$daily at Dokbi (ST35) after inducing MIA). After each operation, the present author observed the motor behavior recovery, hematological (Prostaglandin E2, AST, ALT), histological and immunological changes. Rats were tested at the 7th, 14th and 21st day. Results : Results are as follows. 1. All the experimental groups were improved compared with control group in plantar test. 2. All the experimental groups were improved compared with control group in touch test for sensory evaluator. 3. All the experimental groups were significantly decreased compared with control group in prostaglandin E2. 4. In histological observations, knee joint in all the experimental groups were improved compared with control group. 5. In immunological observations, all the experimental groups were significantly decreased compared with control group in COX-1, 2. Conclusions : It can be suggested that Bee venom and Cervi Cornu Parvum pharmacoacupuncture may improve motor behavior, hematological, histological and immunological findings in MIA-induced osetoarthritis rats. Especially, combination of these two treatments will be somewhat better in osteoarthritis recovery and motor function improvement.

• Journal of Acupuncture Research
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• v.25 no.5
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• pp.105-116
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• 2008

Objectives : The objective of this study is to investigate the suppressing effect of the cervi pantotrichum cornu pharmacopuncture on the expression of iNOS mRNA and production of NO in synoviocytes from artificially arthritis-induced mice. Methods : In vitro test, synoviocytes extracted from a knee joint of a mouse were cultivated, and the herbal extract of cervi pantotrichum cornu($0.4mg/m{\ell}$, $0.6mg/m{\ell}$, $0.8mg/m{\ell}$, and $1.0mg/m{\ell}$) was added into the wells of synoviocytes to suppress the expression of iNOS mRNA and production of NO. In vivo test, each ten mice were allocated into three groups; Normal group, CIA-elicitated group(CIA), and group treated with cervi pantotrichum cornu pharmacopuncture after CIA elicitation(CCA). The extract of cervi pantotrichum cornu was injected into the acupoint of $SP_{10}$ to observe the changes of foot thickness in mice and the suppression of MIF, TNF-$\alpha$, NF-${\kappa}B$ p65, and iNOS. Results : In vitro test, the expression of iNOS mRNA and production of NO were dose-dependently decreased in the wells of synoviocytes treated with PMA. In vivo test, the suppression of MIF, TNF-$\alpha$, NF-${\kappa}B$ p65, and iNOS was clearly shown in the pieces of the synovial joint treated with the extract of cervi pantotrichum cornu. The foot thickness also decreased dose-dependently. Conclusions : It is speculated that the cervi pantotrichum cornu pharmacopuncture can be applicable to the therapy of rheumatoid arthritis by suppressing the expression of iNOS mRNA and production of NO.

• The Journal of Pediatrics of Korean Medicine
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• v.15 no.2
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• pp.75-83
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• 2001

Background and method : The Cervi cornu parvum for children patients have been widely used nowadays: however, there have been no reports about the children patients' conditions and the parents' responses. To investigate these things, the questionnaires were used for the children patients(the ages between 0 and 15) who took the herbal medication combined with Cervix cornu parvum and their parents from January 1, 2000 to June 31 at the Dong-seo oriental Hospital. The chief complaints such as common cold, dyspepsia of using Cervi cornu parvum were divided into five categories and the changes were observed, scoring each stages. Besides, the quantity of taking herbal medication combined with Cervix cornu parvum, the expectancy, the satisfaction, the continual favor were included in the questionnaires. The result : 1. The trackable sample was 53: male infants 34(64.2%), female infants 19(35.8%). There is no differences between genders in the treating results. The treating results were increased after taking herbal medication combined with Cervi cornu parvum 2. When age is grouped by three categories(0-1, 2-6, 7-14 ages), the statistics shows that the treating results have no differences between each group(P>0.05) Regardless of the ages, the treats have correlated.(P<0.05) 3. When each symptoms made treated one case are analyzed, there have been correlations between the taking herbal medication combined with Cervi cornu parvum and recovery.(P<0.05) 4. The treating result have not affected by the expectancy.(P>0.05) Each group(good, moderate, poor) showed the recovery after the treatment.(P<0.05) 5. When the satisfaction divided into three groups(good, moderate, poor) there existed differences between groups.(P<0.05) Especially, the group with high expectancy increased the treatment. 6. About the recognition of herbal medication combined with Cervi cornu parvum is like this: the health refreshment, ordinary taking whenever turning-point of seasons comes, treatment, disbelieving the overdose of herbal medication combined with Cervi cornu parvum, disbelief about the security of the medicine such as fake medicine, mad cow diseases, the imported medicine, ignorance about the way of taking the medicine.

• Korean Journal of Acupuncture
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• v.37 no.2
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• pp.97-103
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• 2020

Objectives : Parkinson's disease, a progressive neurodegenerative disease, is caused by the loss of dopaminergic neurons in the substantia nigra. There is no clear treatment or remedy for Parkinson's disease; therefore, the development of novel therapies related to anti-inflammatory and antioxidant effects is required. This study was performed to evaluate the neuroprotective effect of water extracts from Cervi Cornu (CC) in dopaminergic cells. Methods : We studied effects of CC on apoptosis, cell death and inflammation in SH-SY5Y neuroblastoma cells treated by methylpyridinium ion (MPP+). SH-SY5Y cell line was treated with CC for 24 hours and then 500 μM MPP+ for 18 hours. Results : Cervi Cornu treatment inhibited the decrease in tyrosine hydroxylase (TH) expression and decreased the activation of inflammatory factors mitochondrial cytochrome C oxidase (COX2) and inducible NO synthase (iNOS) against MPP+ neurotoxicity. Apoptosis factors BCL2 associated X, apoptosis regulator (BAX) levels were decreased and B-Cell CLL/Lymphoma 2 (BCL2) levels were increased. Conclusions : These results suggest that CC treatment had neuroprotective effects in the SH-SY5Y neuroblastoma cells against toxicity induced by MPP+. The results suggest new possibilities of CC for the treatment of Parkinson's disease.

• Journal of Pharmacopuncture
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• v.16 no.2
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• pp.7-14
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• 2013

Objective: The endpoint of this review is to investigate existing studies of Cervi cornu parvum (CCP) pharmacopuncture within Korean medicine journals in order to present a better research method in the future. Methods: We searched all the papers through six Korean electrical databases that included the title of "Cervi cornu parvum pharmacopuncture" or "Cervi cornu parvum aqua-acupuncture". Articles that had been published until December 2012 were largely divided into experimental studies and clinical studies. Results: Fifty-three (53) experimental studies and six clinical studies were found. The number of published articles has been constantly increasing. Many of the experimental studies demonstrated anti-inflammatory effects for arthritis, and most of the clinical studies dealt with musculoskeletal problems. Conclusion: Various therapeutically significant effects of the CCP pharmacopuncture have been found through this review; however, more systematic clinical studies on the CCP pharmacopuncture seem to be necessary to substantially support its clinical effects.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.5
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• pp.1194-1199
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• 2007

Osteoarthritis(OA) is a degenerative joint disease characterized by fibrillation and erosion in cartilage tissue, chondrocyte proliferation and osteophyte formation at the joint margins, and sclerotis of subchondral bone. We investigated the effects of Acyranthes Radix administration and Cervi Cornu Parvum aqua-acupuncture in monosodium iodoacetate(MIA) induced experimental osteoarthritis model. Sprague-Dawley 60 rats of 7-8 weeks, weight $240{\pm}10\;g$ were divided into two groups including the sham operation group(15 rats) and ostoarthritis group(45 rats). Histopathological examination, Mankin's score, and the measurement of inflammation factor were performed. Histological findings that are similar to those observed in human osteoarthritis, such as disorganization of chondrocytes, erosion and fibrillation of cartilage surface, and subchondral bone exposure were observed in a MIA-induced osteoarthritis model. Saflanin-O fast green staining revealed that marked diffuse reduction of proteoglycans treated with MIA. The Mankin's score were closely correlated to the grade of histological findings. The level of prostaglandin E2 and C-reactive protein were decreased experimental groups. We conclude that Acyranthes Radix administration and Cervi Cornu Parvum aqua-acupuncture, and combination treatment exerts a beneficial influence on the cartilage lesion in osteoarthritis rat.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.2
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• pp.249-255
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• 2006

Object : The tinnitus is a very common otorhinolaryngologic disease. But, we don't know the exact cause and the healing method of that. Treatment : We have three cases report of the patients who have tinnitus. Their tinnitus is diagnosed weakness of the kidney because of chronic symptoms, the Cheek pulse weakening, the whole body weakness, fatigue. So we treat them with herbal medicine and Cervi Pantotricuhum Cornu herbal-acupunture. Result : In the result, their symptoms is reduced. So we report these cases for the improvement of tinnitus treatment efficacy and making the best use of Cervi Pantotricuhum Cornu herbal-acupunture.

• Journal of Acupuncture Research
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• v.18 no.1
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• pp.61-75
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• 2001

Objectives : This study was designed to evaluate on the effects of the Carthami semen Cervi pomtotrichum cornu Hominis placenta aquacupuncture on the osteoporosis in the rats Materials and Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then aquacupunctures were injected into the pai shu points corresponding to urinary bladder meridian of human body in experimental groups. And such indexes were measured as the change of body weight, bone mineral density, histological change of tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus in serum. Results : 1. Significant changes of trabecular area % in epiphysis of tibia were shown between control group and experimental group II and between experimental group I and group II. 2. Significant changes of trabecular area % in diaphysis of tibia were shown between control and experimental groups. 3. Significant changes of serum osteocalcin were shown between control group and experimenta group I . 4. Significant changes of levels of serum bone alkaline phosphatase were shown between control group and experimental group I and II. Conclusions : The Carthami semen Cervi pomototrichum cornu Hominis placenta aquacupuncture is effective to estrogen deficient osteoporosis, ovariectomy was done on rats.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.4
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• pp.1-16
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• 2008

Purpose: The purpose of this study is to investigate the effect of Boheo-tang (B) and Boheo-tang plus cervi pantotrichum cornu (B+CP) on lactation in postpartum C57BL/6N mice. Methods: Normal saline(control), Band B+CP (8mu l/g$) were administerd p.o. twice a day for 20 days. Lactating mammary gland tissues were examined through light microscope by the way of HE staining and immunohistochemical assay. Milk producing associated gene expression were accessed by RT-PCR. Results: In mammary gland, amount of adipose tissues were decreased in both Band B+CP treated group. And the ductal branches and alveolar tissues increased in both treated group. Immunoreactivity of prolactin receptors was increased both treated group, and immunoreactivity of oxytocin receptors was increased in the B+CP treated group. In both treated group, IGF-l mRNA expression was increased and TGF-$\beta$ mRNA expression was decreased. And PRL mRNA expression was increased in the B+CP treated group. PL-l mRNA expression was decreased in the B treated group but increased in the B+CP treated group. Conclusion: This study shows that treatment of Boheo-tang and Boheo-tang plus cervi pantotrichum cornu can improve postpartum lactation in C57BL/6N mice.