• Title/Summary/Keyword: Center of Mass

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Characterization of Yeast and Bacterial Type Strains with Food and Agricultural Applications by MALDI-TOF Mass Spectrometry Biotyping

  • Harnpicharnchai, Piyanun;Jaresitthikunchai, Janthima;Seesang, Mintra;Jindamorakot, Sasitorn;Tanapongpipat, Sutipa;Ingsriswang, Supawadee
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.138-147
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    • 2020
  • Various microorganisms play important roles in food fermentation, food spoilage, and agriculture. In this study, the biotype of 54 yeast and bacterial strains having high potential for utilization in food and agriculture, including Candida spp., Lactobacillus spp., and Acetobacter spp., were characterized by matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS). This characterization using a fast and robust method provides much-needed information on the selected microorganisms and will facilitate effective usage of these strains in various applications. Importantly, the unique protein profile of each microbial species obtained from this study was used to create a database of fingerprints from these species. The database was validated using microbial strains of the same species by comparing the mass spectra with the created database through pattern matching. The created reference database provides crucial information and is useful for further utilization of a large number of valuable microorganisms relevant to food and agriculture.

Traumatic Pseudoaneurysm of the Superficial Temporal Artery due to Gardner Traction

  • Lee, Hyun-Seok;Jo, Kwang-Wook;Lee, Sun-Ho;Eoh, Whan
    • Journal of Korean Neurosurgical Society
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    • v.48 no.3
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    • pp.291-293
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    • 2010
  • We report a case of pseudoaneurysm of the parietal division of the superficial temporal artery (STA) secondary to iatrogenic head injury due to Gardner traction. A 54-year-old man presented with a pulsatile, cystic, and painless mass in the right anterior temporal region which developed three weeks after head fixation via Gardner traction. At the time of discovery, the mass was 10 mm in diameter, compressible and disappeared after manual compression of the proximal STA. A bruit was audible over the mass, which was thought to be a pseudoaneurysm. A computed tomography angiogram (CTA) showed a pseudoaneurysm of the parietal division of the right ST A. The tip of the pseudoaneurysm was thrombosed and was both red and tender. The pseudoaneurysm was thought to be filled with infected thrombus, and the mass was resected with ligation of the proximal and distal ends of the STA. A pseudoaneurysm of the STA should be suspected when there is a history of possible vessel injury, such as a history of head-pin fixation, and when a patient presents with a pulsatile, cystic mass near the temple. Pseudoaneurysms can be successfully treated by excision.

Observation of Peptide-Ion Generation by Laser-Induced Surface Heating from Tungsten Silicide Surfaces

  • Kim, Shin-Hye;Park, Sun-Hwa;Song, Jae-Yong;Han, Sang-Yun
    • Mass Spectrometry Letters
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    • v.3 no.1
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    • pp.18-20
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    • 2012
  • We report observation of laser desorption/ionization (LDI) of peptides from flat surfaces of tungsten silicide ($WSi_2$). In contrast to MALDI (matrix-assisted laser desorption/ionization) and SALDI (surface-assisted laser desorption/ionization) mass spectrometry, this study did not utilize any matrices and surface nanostructures. In this work, LDI on $WSi_2$ surfaces is demonstrated to cover a mass range up to 1,600 Da (somatostatin; monoisotopic mass = 1637.9 Da). In addition, it exhibited a high sensitivity, which could detect peptides, which could detect peptides of low femtomole levels (20 fmol for angiotensin II). The observed LDI process was discussed to be largely thermal, more specifically, due to laser-induced surface heating that is most likely promoted by the low thermal diffusivity (${\kappa}$) of $WSi_2$ substrate.

On the Origin of the Correlation between Hubble Residual and Mass of the Type Ia Supernova Host Galaxies

  • Kang, Yijung;Kim, Young-Lo;Lim, Dongwook;Chung, Chul;Lee, Young-Wook
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.36.1-36.1
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    • 2014
  • The correlation between mass of Type Ia Supernova (SN Ia) host galaxies and Hubble residual is now well-established. The origin of this relation, however, is yet to be understood. We have used low-resolution spectra of early-type hosts from YONSEI (YOnsei Nearby Supernovae Evolution Investigation) project to measure central velocity dispersion and Lick/IDS absorption indices. By using the Evolutionary Population Synthesis (EPS) models, luminosity-weighted mean age and metallicity of host galaxies were determined from $H{\beta}$ and absorption lines. Here we will discuss the correlation between the velocity dispersion, which indicates the mass of galaxies, and mean age of stellar population in our sample of early-type host galaxies.

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A Study on Modeling of LD Movement and Measurement of Mass Center using Piezoelectric Element (압전소자를 이용한 레이저디스크 이동현상의 모델링과 질량중심의 위치결정에 관한 연구)

  • Song, Hwa-Seop;Hong, Jun-Hee
    • Journal of the Korean Society for Precision Engineering
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    • v.16 no.4 s.97
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    • pp.213-219
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    • 1999
  • Piezoelectric element is deformed by driving voltage pattern. We developed a device of moving LD(laser disk) to use the rapid deformation of piezoelectric element. If driving voltage is changed very rapidly, the difference of acceleration is accurred between spindle motor and LD. To move LD on turn table is attained by utilizing difference of acceleration. This paper describes theoretical and experimental results about the movement of LD and presents to measure the center of mass LD with unbalance force.

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MALT Lymphoma of Ocular Adnexa: A Case Report (안구 부속기의 점막연관 림프조직형 림프종의 증례보고)

  • Cho, Jeong Nam;Kim, Yoong Soo;Chung, Chan Min;Suh, In Suck;Cho, Ji Woong;Park, Hye Rim;Choi, Jae Gu
    • Archives of Plastic Surgery
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    • v.35 no.3
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    • pp.321-324
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    • 2008
  • Purpose: Lymphoma originated from mucosa associated lymphoid tissue(MALT) is most common in gastrointestinal system, and rarely found in salivary gland, thyroid, bronchus or orbit. We experienced a case of MALT lymphoma which was originated from conjunctiva and involving lower eyelid without metastasis. Methods: A 40-year-old man suffered palpable mass on right lower eyelid without pain. Orbital computed tomographic and ultrasonographic findings showed a conical mass($1.9{\times}1.2{\times}0.9cm$ size) inside lower eyelid. The mass was completely excised under local anesthesia and histopathological examination was followed. Results: Microscopic finding showed a multiple follicular colonization. In the follicle, small lymphocytes and plasma cells differentiated to centrocyte-like cell, monocyte B cell, plasma cell were diffusely infiltrated. Immunophenotyping was preformed on fixed section. The majority of the small cells were immunoreactive for the B cell marker CD20. Based on the typical histological findings supported by immunostaining, the mass was defined as MALT lymphoma. After excision, SPECT, abdominal CT was carried out and there were no evidence of extraorbital disease. Conclusion: Biopsy and pathological examination should be performed in patients who complain palpable mass on lower eyelid because of possibility of MALT lymphoma. Although MALT lymphoma is rarely metastasized, it is necessary to evaluate the extraorbital involvement using SPECT or other radiologic exams. For detecting extraorbital involvement, periodic follow-up examination is need.

External and Internal Glucose Mass Transfers in Succinic Acid Fermentation with Stirred Bed of Immobilized Actinobacillus succinogenes under Substrate and Product Inhibitions

  • Galaction, Anca-Irina;Rotaru, Roxana;Kloetzer, Lenuta;Vlysidis, Anestis;Webb, Colin;Turnea, Marius;Cascaval, Dan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1257-1263
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    • 2011
  • This paper is dedicated to the study on the external and internal mass transfers of glucose for succinic acid fermentation under substrate and product inhibitions using a bioreactor with stirred bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the kinetic model adapted for both inhibitory effects, specific mathematical models were developed for describing the profiles of the substrate concentration in the outer and inner regions of biocatalysts and for estimating the substrate mass flows in the liquid boundary layer surrounding the particle and inside the particle. The values of the mass flows were significantly influenced by the internal diffusion velocity and rate of the biochemical reaction of substrate consumption. These cumulated influences led to the appearance of a biological inactive region near the particle center, its magnitude varying from 0 to 5.3% of the overall volume of particles.

A Changing Pattern of Isotope Scan of the Midline Ectopic Thyroid - A Case Report - (정중선 이소성 갑상선에서 주사상의 변화 추적 - 증례보고 -)

  • Kim, Kap-Tae;Kim, Wan-Sub;Chung, Eul-Sam
    • Advances in pediatric surgery
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    • v.2 no.2
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    • pp.133-137
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    • 1996
  • A 17 month-old girl presented in the pediatric clinic on November 27th, 1990 with a neck mass. The mass was 2 cm in diameter, firm in consistency and movable on the upper pole of the thyroid cartilage in the midline. The technetium thyroid scan showed a hot reactivity at the compatible site of the mass, but no other radioactivity in either site of the normal thyroid positions. At her second visit on January 23th, 1996, the mass had enlarged up to 3.5cm in diameter in the same location of the neck. The follow up thyroid scan revealed a walnut sized, snowman-like radioactivity. One of the snowman-like double images seemed to be a lingual ectopic thyroid and the other a midline ectopic thyroid remnant in the infrahyoid level. This interpretation was supported by the computed tomography of the neck, which showed a ligual mass in the foramen cecum area and an another mass in the anterior comis-sure of the larynx in the mildline. Thyroid function test was normal except a slightly increased TSH. As a result of this changing pattern of thyroid radioactive images, a case of a lingual thyroid as well as another midline ectopic thyroid tissue at the infrahyoid level is reported.

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Cystic Mass on Right Atrium of Unusual Form of Chiari's Network: A Case Report

  • Bae, Chi-Hoon;Kwon, Oh-Choon;Lee, Sub;Lee, Chul-Ho;Cho, Jun-Woo
    • Journal of Chest Surgery
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    • v.45 no.4
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    • pp.254-256
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    • 2012
  • Chiari's networks are present in 1.5% to 4% of the population. They are a congenital disease characterized by a remnant of the right valve of sinus venosus and rarely have clinical significance. Chiari's network, as the name implies, has network-like shape, but there are other forms of appearance. We have experienced a case of a 60-year-old woman who had a cystic mass on the right atrium. Surgical treatment was performed forthe mass removal and differential diagnosis of the mass. There was no evidence of other tumor, but Chiari's network. As cystic form of Chiari's network have not been reported before, it is the first report of cystic form of Chiari's network.

Development of Ultra-High Pressure Capillary Reverse-Phase Liquid Chromatography/Tandem Mass Spectrometry for High-Sensitive and High-Throughput Proteomics

  • Kim, Min-Sik;Choie, Woo-Suk;Shin, Yong-Seung;Yu, Myeong-Hee;Lee, Sang-Won
    • Bulletin of the Korean Chemical Society
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    • v.25 no.12
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    • pp.1833-1839
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    • 2004
  • Recently mass spectrometry and separation methods such as liquid chromatography have become major tools in the field of proteomics. In this report, we describe in detail our efforts to develop ultra-high pressure capillary reverse-phase liquid chromatography (cRPLC) and its online coupling to a mass spectrometer by a nanoelectrospray (nanoESI) interface. The RPLC system is constructed in house to deliver LC solvents at the pressure up to 20,000 psig, which is four times higher than conventional RPLC systems. The high operation pressure allows the efficient use of packed micro-capillary columns (50, 75 and 150 ${\mu}$m i.d., up to 1.5 m long). We will discuss the effect of column diameter on the sensitivity of cRPLC/MS/MS experiments and the utility of the developed technique for proteome analysis by its application in the analysis of proteome samples having different levels of complexity.