• The Plant Pathology Journal
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• v.39 no.4
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• pp.309-318
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• 2023

Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, 'Candidatus Liberibacter asiaticus' (CLas) is a non-culturable phloem-limited α-proteobacterium transmitted by Asian citrus psyllids (ACP, Diaphorina citri Kuwayama). A widely adopted method for HLB diagnosis is based on quantitative real-time polymerase chain reaction (qPCR). Although HLB diagnostic qPCR provides high sensitivity and good reproducibility, it is limited by time-consuming DNA preparation from plant tissue or ACP and the requirement of proper lab instruments including a thermal cycler to conduct qPCR. In an attempt to develop a quick assay that can be deployed in the field for CLas detection, we developed a real-time loop-mediated isothermal amplification (rt-LAMP) assay by targeting the CLas five copy nrdB gene. The rt-LAMP assay using various plant sample types and psyllids successfully detected the nrdB target as low as ~2.6 Log10 copies. Although the rt-LAMP assay was less sensitive than laboratory-based qPCR (detection limit ~10 copies), the data obtained with citrus leaf and bark and ACP showed that the rt-LAMP assay has >96% CLas detection rate, compared to that of laboratory-based qPCR. However, the CLas detection rate in fibrous roots was significantly decreased compared to qPCR due to low CLas titer in some root DNA sample. We also demonstrated that the rt-LAMP assay can be used with a crude leaf DNA extract which is fully deployable in the field for quick and reliable HLB screening.

• Journal of Information Processing Systems
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• v.10 no.1
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• pp.1-22
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• 2014

Recent technological advances provide the opportunity to use large amounts of multimedia data from a multitude of sensors with different modalities (e.g., video, text) for the detection and characterization of criminal activity. Their integration can compensate for sensor and modality deficiencies by using data from other available sensors and modalities. However, building such an integrated system at the scale of neighborhood and cities is challenging due to the large amount of data to be considered and the need to ensure a short response time to potential criminal activity. In this paper, we present a system that enables multi-modal data collection at scale and automates the detection of events of interest for the surveillance and reconnaissance of criminal activity. The proposed system showcases novel analytical tools that fuse multimedia data streams to automatically detect and identify specific criminal events and activities. More specifically, the system detects and analyzes series of incidents (an incident is an occurrence or artifact relevant to a criminal activity extracted from a single media stream) in the spatiotemporal domain to extract events (actual instances of criminal events) while cross-referencing multimodal media streams and incidents in time and space to provide a comprehensive view to a human operator while avoiding information overload. We present several case studies that demonstrate how the proposed system can provide law enforcement personnel with forensic and real time tools to identify and track potential criminal activity.

• Bulletin of the Korean Chemical Society
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• v.32 no.spc8
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• pp.2928-2932
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• 2011

A chemosensor based on a rhodamine-hydroxamate platform containing a pyridine and a cyclen binding units has been developed for the detection of $Cd^{2+}$ in aqueous solutions. The probe responds selectively toward $Cd^{2+}$ over other biologically relevant metal ions. The fluorescent probe shows 1:1 binding stoichiometry and the detection limit for $Cd^{2+}$ in water proved to be as low as 25 nM.

• Proceedings of the IEEK Conference
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• 2007.07a
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• pp.287-288
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• 2007

In this paper, we propose a fake-iris detection method. In order to detect the fake-iris, we measure physiological features which are the reflectance ratio of the iris to the sclera at 750 nm and that at 850nm. In order to classify live and fake iris features, we use support vector data description (SVDD). From our experimental results, it is clear that our fake-iris detection method achieves high performance when distinguishing between a live-iris and a fake-iris.

• ETRI Journal
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• v.38 no.4
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• pp.599-605
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• 2016

For the implementation of a real-time holographic camera, fast and automatic holographic image reconstruction is an essential technology. In this paper, we propose a new automatic depth-detection algorithm for fast holography reconstruction, which is particularly useful for optical scanning holography. The proposed algorithm is based on the inherent phase difference information in the heterodyne signals, and operates without any additional optical or electrical components. An optical scanning holography setup was created using a heterodyne frequency of 4 MHz with a 500-mm distance and 5-mm depth resolution. The reconstruction processing time was measured to be 0.76 s, showing a 62% time reduction compared to a recent study.

• Journal of the Korean Magnetic Resonance Society
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• v.24 no.2
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• pp.59-65
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• 2020

Nitrogen-Vacancy (NV) center in diamond has been an emerging versatile tool for quantum sensing applications. Amongst various applications, nano-scale nuclear magnetic resonance (NMR) using a single or ensemble NV centers has demonstrated promising results, opening possibility of a single molecule NMR for its chemical structural studies or multi-nuclear spin spectroscopy for quantum information science. However, there is a key challenge, which limited the spectral resolution of NMR detection using NV centers; the interrogation duration for NV-NMR detection technique has been limited by the NV sensor spin lifetime (T₁ ~ 3ms), which is orders of magnitude shorter than the coherence times of nuclear spins in bulk liquid samples (T₂ ~ 1s) or intrinsic ¹³C nuclear spins in diamond. Recent studies have shown that quantum memory technique or synchronized readout detection technique can further narrow down the spectral linewidth of NMR signal. In this short review paper, we overview basic concepts of nanoscale NMR using NV centers, and introduce further developments in high spectral resolution NV NMR studies.

• Proceedings of the KIPE Conference
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• 2008.06a
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• pp.634-636
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• 2008

This paper proposes a frequency detection method based on an advanced zero-crossing technique. Zero-crossing method for detecting frequency is one of the most widely used methods today. Although it is simple to apply, it requires extra hardware in implementation due to its limitations in accuracy. The proposed method models the error generated during zero crossing linearization and compensated for it in real time which makes it simple and accurate. The validity of the method and its applicability in anti-islanding detection of distributed generators was confirmed through simulation.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.165-166
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• 2008

Bistatic spotlight synthetic aperture radar(BSSAR) with single track configuration uses the transmitter and the receiver which travel along the single track such as the leader-follower. For the BSSAR imaging, we modify the range migration algorithm. In time domain, we make the monostatic SAR using shifting of path, Then, in frequency domain, we compensate the separated distance between the scene center and the flight path using the principle of the stationary phase (PSP).

• Proceedings of the SAREK Conference
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• 2006.06a
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• pp.807-813
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• 2006

The objective of this study is to develop a rule-based fault detection algorithm and an experimental verification using an artificial air handling unit. To develop an analytical algorithm which precisely detects a tendency of faulty component, energy equations at each control volume of AHU were applied. An experimental verification was conducted on the HVAC simulator. The rule based FDD algorithm isolated a faulted sensor from HVAC components in summer and winter conditions.

• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.459-468
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• 2020

This study established a new polymerase spiral reaction (PSR) that combines with reverse transcription reactions for HCV detection targeting 5'UTR gene. To avoid cross-contamination of aerosols, an isothermal amplification tube (IAT), as a separate containment control, was used to judge the result. After optimizing the RT-PSR reaction system, its effectiveness and specificity were tested against 15 different virus strains which included 8 that were HCV positive and 7 as non-HCV controls. The results showed that the RT-PSR assay effectively detected all 8 HCV strains, and no false positives were found among the 7 non-HCV strains. The detection limit of our RT-PSR assay is comparable to the real-time RT-PCR, but is more sensitive than the RT-LAMP. The established RT-PSR assay was further evaluated for detection of HCV in clinical blood samples, and the resulting 80.25% detection rate demonstrated better or similar effectiveness compared to the RT-LAMP (79.63%) and real-time RT-PCR (80.25%). Overall, the results showed that the RT-PSR assay offers high specificity and sensitivity for HCV detection with great potential for screening HCV in clinical blood samples.