• 제목/요약/키워드: Cellulose I

검색결과 385건 처리시간 0.028초

Energy Expenditure in Crossbred Cattle Fed Paddy Straw of Different Form

  • Bhatta, Raghavendra;Kumar, Vijay;Sridhar, Manpal;Singh, Khub
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제19권12호
    • /
    • pp.1755-1760
    • /
    • 2006
  • Studies were carried out at the National Institute of Animal Nutrition and Physiology, India to determine the effect of feeding chopped paddy straw (Oryza sativa) on the energy expenditure in crossbred cattle. Four crossbred cattle male, aged 5-6 years and weighing about 450 kg were used for this study. Three experimental trials, one each for the feeding of un-chopped paddy straw offered ad libitum (UCA), chopped paddy straw fed at restricted level (CR) and chopped paddy straw offered ad libitum (CA) were conducted. The quantity of un-chopped paddy straw consumed during UCA was assumed as the voluntary intake by the cattle and the same quantity was offered after chopping during CR. Each trial comprised of 21 d preliminary feeding period and 5 d of observation recording period. Expired gas was collected in Douglas bags using a face-mask and three-way valve at 6 hourly intervals i.e., at 09.30, 15.30, 21.30, and 03.30 h throughout the observation period. Expired gas and ambient air inspired by the animals were analyzed for the oxygen content through paramagnetic oxygen analyzer. Energy expenditure (EE) by the animals was calculated by determining the volume of oxygen consumed per minute (STP) and multiplying by 4.825. Paddy straw used in all the three trials contained (g/kg DM) 90.0 CP, 786 OM, 700 NDF, 489 ADF, 357 Cellulose and 60.0 ADL. Metabolizable energy (ME) was 6.9 MJ/kg DM. Dry matter intake (DMI) both in UCA and CR was about 6.8 kg, except that it was chopped in CR. Chopping has resulted in 32% improvement (9 kg) in DMI of CA as compared to that of UCA. Although ME intake was similar in UCA and CR (47.2 MJ/day), energy expenditure (EE) was higher in UCA (23.3 MJ) when compared to that of CR (19.5 MJ). The ME intake (63.3 MJ) as well as EE (27.1 MJ) was highest in CA. Energy expenditure when expressed as MJ/kg DMI was 3.48, 2.90 and 3.12; whereas as per cent of ME intake it was 50, 41 and 44 in UCA, CR and CA respectively. Our study has unequivocally confirmed that chopping of poor quality roughages like paddy straw has definite advantages not only in terms of improving the intake by decreasing the time taken for ingestion but also in reducing the energy cost of eating.

Chemical Properties and Fiber Dimension of Eucalyptus pellita from The 2nd Generation of Progeny Tests in Pelaihari, South Borneo, Indonesia

  • Lukmandaru, Ganis;Zumaini, Umi Farah;Soeprijadi, Djoko;Nugroho, Widyanto Dwi;Susanto, Mudji
    • Journal of the Korean Wood Science and Technology
    • /
    • 제44권4호
    • /
    • pp.571-588
    • /
    • 2016
  • Eucalyptus pellita F. Muell is one of pulp woods that is being developed through breeding plantation programs in Indonesia. The research aimed at exploring the chemical and morphological characteristics of fiber, and to determine the rank of plus trees from 4 provenances based on the suitability for pulps. The materials included the plus trees of E. pellita (9 years) from the 2nd generation of progeny tests in Pelaihari, South Borneo. Wood properties under investigation included the chemical properties and morphological fiber characteristics (fiber dimensions and its derived properties). In the present study, data were analyzed using descriptive statistic, Analytic Hierarchy Process (AHP) and Pearson's correlation. Results showed that the chemical properties of E. pellita, i.e. the contents of ethanol-toluene extractives, hot water soluble extractives, holocellulose, alphacelullose, and lignin were $3.08{\pm}1.00%$, $1.41{\pm}0.38%$, $75.26{\pm}2.58%$, $49.02{\pm}2.88%$, and $29.49{\pm}1.86%$, respectively. The average values of wood fiber morphology were $1.02{\pm}0.08$ mm (fiber length), $13.25{\pm}1.64{\mu}m$ (fiber diameter), of $6.94{\pm}1.70{\mu}m$ (lumen diameter), $3.15{\pm}0.52{\mu}m$ (fiber wall thickness), $0.97{\pm}0.30$ (Runkel ratio), $0.57{\pm}0.10$ (Luce's shape factor), $78.21{\pm}10.34$ (slenderness ratio) and $130.91{\pm}33.77{\times}10^3{\mu}m^3$ (solids factor). The AHP scoring rank indicated that the best individuals were 28.4.3.28 (Kiriwo Utara), 12.1.5.28 (North Kiriwo), 19.11.5.45 (Serisa Village), 3.8.4.9 (South Kiriwo), and 6.6.3.15 (South Kiriwo). Pearson correlation analysis showed significant correlations between the levels of fiber length with alpha-cellulose content (r = 0.39) as well as the fiber length with ethanol-toluene extractive contents (r = -0.41).

오디(Mulberry) 추출물의 성분분석 및 생리활성에 관한 연구 (Studies on the Chemical Constituents Biological Activities of Mulberry Extracts)

  • 이상극;이학주;강하영;최돈하;조현진;이태성
    • Journal of the Korean Wood Science and Technology
    • /
    • 제37권1호
    • /
    • pp.105-113
    • /
    • 2009
  • 동결 오디(Mulberry) 10 kg을 80% 에탄올(EtOH)로 추출하고 분획깔때기 상에서 헥산(n-hexane), 에틸아세테이트(EtOAc) 및 물 가용부로 분획하였다. 에틸아세테이트 가용부(22 g)를 Sephadex LH-20으로 충진한 칼럼에서 MeOH-$H_2O$ (2 : 1~1 : 3, v/v)을 용출용매로 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 셀룰로오스 박층크로마토그래피(TLC)로 확인한 후 $^1H-$, $^{13}C$-NMR 등의 스펙트럼을 사용하여 정확한 구조를 구명하였고 FAB 및 EI-MS로써 분자량을 측정하였다. 오디의 에틸아세테이트 가용부에서 quercetin-3-O-${\beta}$-D-glucopyranoside (화합물 1), protocatechuic acid (화합물 2), p-hydroxybenzoic acid (화합물 3)을 단리할 수 있었다. 항산화 시험 결과 에틸아세테이트와 물 가용부에서 BHT보다 우수한 활성을 나타내었으며 MTT assay에 의한 세포생존율 측정결과 세포독성은 없는 것으로 나타났다. 또한, 미백활성 및 항암활성은 낮았지만 세포신장 효과가 우수하여 주름개선 화장품 소재로서 충분히 이용 가능성이 있는 것으로 판단되어 진다.

Ru-Mg-Al-oxide 촉매 상에서 크라프트 리그닌의 저분자화 연구 (Depolymerization of Kraft Lignin over a Ru-Mg-Al-oxide Catalyst)

  • 김한웅;수잔 올리비아;제정호
    • 청정기술
    • /
    • 제27권2호
    • /
    • pp.190-197
    • /
    • 2021
  • 펄프 및 제지산업에서 목재의 셀룰로오스 성분 활용 후 남는 부산물인 크라프트 리그닌(kraft lignin)은 촉매적 저분자화 공정을 통해 바이오연료나 고부가가치 페놀 단량체로 전환될 수 있다. 본 연구에서는 크라프트 리그닌의 효율적인 저분자화를 위한 촉매로 수소화 금속 및 산-염기점을 동시에 지니는 Ru-Mg-Al-oxide 복합 촉매를 제조하고, 리그닌 분해 성능을 평가하고자 하였다. 촉매 내 다양한 활성점들(산점, 염기점, 수소화 금속)이 리그닌 분해 반응에 미치는 영향을 파악하기 위해 MgO, Mg-Al-oxide, Ru-Mg-Al-oxide의 세 가지 촉매를 제조하여 초임계 에탄올 용매 상에서 리그닌 분해 반응을 수행하였고, 리그닌 분해 성능은 바이오오일(bio-oil) 수율 및 분자량, 그리고 페놀계 단량체 수율을 통해 평가하였다. 그 결과, Ru-Mg-Al-oxide 촉매가 다양한 활성점들의 시너지 효과로 인해 가장 높은 수율의 바이오오일 및 페놀 단량체들을 생산한다는 것을 확인하였다. Ru-Mg-Al-oxide 촉매 상에서 분해 효율을 최적화하기 위해 다양한 반응 조건(온도, 시간, 촉매양)에 따른 분해 효율을 평가하였고, 최종적으로 반응온도 350 ℃, 리그닌 대비 촉매 비율 10%, 4 h 반응을 통해 72%의 높은 바이오오일 수율과 무촉매 대비 3.5배 이상 증가한 페놀 단량체를 생산할 수 있었다.

Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses

  • Lee, Imchang;Podolich, Olga;Brenig, Bertram;Tiwari, Sandeep;Azevedo, Vasco;de Carvalho, Daniel Santana;Uetanabaro, Ana Paula Trovatti;Goes-Neto, Aristoteles;Alzahrani, Khalid J.;Reva, Oleg;Kozyrovska, Natalia;de Vera, Jean-Pierre;Barh, Debmalya;Kim, Bong-Soo
    • Journal of Microbiology and Biotechnology
    • /
    • 제32권8호
    • /
    • pp.967-975
    • /
    • 2022
  • Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers.

Tragacanth gum 의 신다당류(新多糖類) C 의 화학구조(化學構造) - Tragacanth gum의 신다당류(新多糖類)에 관(關)한 연구(硏究) 제2보(第二報) - (Studies on the Chemical Structure of the New Polysaccharide C - (The New Polysaccharides of Gum Tragacanth. II) -)

  • 이성환
    • Applied Biological Chemistry
    • /
    • 제3권
    • /
    • pp.25-48
    • /
    • 1962
  • tragacanth gum의 화학구조(化學構造)를 구명(究明)하기 위(爲)하여 미국(美國) 약전(藥典)의 tragacanth gum 분말(粉末)을 가지고 다음의 실험(實驗)을 통(通)하여 이의 성분(成分)의 하나인 polysaccharide C를 분리(分離)하여 이의 화학구조(化學構造)를 밝혔다. (1) tragacanth gum을 85% 주정(酒精)으로 처리(處理)해서 중성다당류(中性多糖類)로 polysaccharide C를 분리(分離)하였으며 구성당(構成糖)으로 L-rhamnose, D-xylose, L-arabinose 및 D-galactose를 paper chromatography와 Cellulose column chromatography로 분리(分離), 동정(同定)하였다. 이의 molar ratio는 2:1:17:9이며 비선광도(比旋光度)는 $[{\alpha}]^{30}_D-72.2이다. (2) 구성당(構成糖)의 결합위치(結合位置)를 구명(究明)하기 위(爲)해 Hawarth 법(法)과 Purdietldir(試藥)을 가지고 methyl화(化)시켜 methyl화(化) polysaccharide C를 얻었으며 비선광도(比旋光度) $[{\alpha}]^{22}_D-102를 보였다. 이것을 가수분해(加水分解)시켜 paper chromatography와 column chromatography를 통(通)해 methyl화단당(化單糖)으로 1,3,5-tri-O-methyl-L-arabofuranose, 3,4-di-O-methyl-L-rhamnose, 2,3-di-O-methyl-D-xylose, 2,3,4-tri-O-methyl-D-galactopyranose, 2,4-di-O-methyl-L-arabopyronose, 2,4-di-O-methyl-D-galactose, 2-O-methyl-L-arabinose 및 L-arabinose를 분리(分離), 동정(同定)하였다. (3) 산(酸)의 각종농도(各種濃度)에 따른 부분적(部分的) 가수분해(加水分解)를 시켜 polysaccharide C의 end group, 측지(側枝) 또는 주쇄(主鎖)를 이루는 구성당(構成糖)을 밝히기 위(爲)하여 0.05 N-HCl로 제1차(第一次) 가수분해(加水分解). 0.1N-HCl로 제2차(第二次) 가수분해(加水分解), 0.3N-HCl로 제3차(第三次) 가수분해(加水分解)를 하여 가수분해물(加水分解物)과 비가수분해물(非加水分解物)에서 각각(各各) 다음과 같은 구성단당(構成單糖)을 검출(檢出)하고 이들의 molar ratio를 측정(測定)하였다. 제1차(第一次) 가수분해물(加水分解物)(A)에서 L-arbinose, 비가수분해물(非加水分解物)(A')에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose; 제2차(第二次) 가수분해물(加水分解物)(B)에서 L-arabinose와 D-galactose, 비가수분해물(非加水分解物)(B')에서 L-rhamnose, D-xylose, L-arabinose, 및 D-galactose; 제3차(第三次) 가수분해물(加水分解物)(C)에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose, 비가수분해물(非加水分解物)(C')에서 D-xylose와 D-galactose를 검출(檢出)하였다. (4) 구성당(構成糖)의 형태(形態)와 구조(構造)를 밝히기 위(爲)해 polysaccharide C에 대한 periodate산화(酸化) 실험(實驗)을 하여 $C_5H_8O_4$당(當) periodate의 소비(消費)와 formic acid의 생성량(生成量)을 측정(測定)하였는데 periodate의 소비량(消費量)은 1.23 mole, formic acid의 생성량(生成量)은 0.78 mole이다.

  • PDF

Cellulase에 의(依)한 목재당화(木材糖化)에 관(關)한 연구(硏究) - (I) 기질(基質) 처리(處理)의 효과(効果) - (Studies on the Hydrolysis of Holocellulose with Trichoderma viride Cellulase - (I) Effect of the treated substrate -)

  • 정대성;민두식
    • 한국산림과학회지
    • /
    • 제38권1호
    • /
    • pp.13-18
    • /
    • 1978
  • Trichoderma viride 16374호(號) Cellulase에 의(依)한 목재가수분해(木材加水分解)에 관(關)한 연구(硏究)로서 재료(材料)는 산오리나무재(材)를 사용(使用)하였다. 효소생산(酵素生産)은 액체(液體) 진탕배양법(振盪培養法)에 의(依)하였다. 즉(即) 밀기울 추출액(抽出液)에 Pulp분말(粉末)(Toyo여지(濾紙) 60 mesh) 10, $KH_2PO_410$, $(NH_4)_2$ $SO_4$ 3, $NaNO_3$ 3, $MgSO_4$ $7H_2O$ 0.5g/1을 가(加)하였다. 이와 같이 하여 진탕배양(振盪培養)한 후(後) 배양액(培養液)을 취(取)하여 유안포화도(硫安飽和度)에 의(依)한 염석조효소액(鹽析粗酵素液)을 만들었다. 환원당정량(還元糖定量)은 DNS법(法)에 의(依)하였다. (1) 탈(脫)리그닌은 외산(外山)(1970)의 과초산법(過醋酸法)에 의(依)하였다. 여기서 과초산(過醋酸)의 농도(濃度)가 높을수록 단기간(短期間)에 Pulp수율(收率)은 감소(減少)하였다. 즉(即) 20% 과초산액(過醋酸液)으로 처리(處理)한 시료(試料)는 48시간(時間)에, 40% 및 6% 과초산액(過醋酸液)으로 처리(處理)한 시료(試料)는 24시간(時間)이면 충분(充分)한 탈(脫)리그닌이되었다. (2) 기질(基質)은 징세(徵細)할수록 효소(酵素)에 의(依)한 가수분해(加水分解)가 용이(容易)하였으며 환원당생성(還元糖生成)에 적합(適合)한 기질(基質)의 크기는 60-100mesh로 나타났다. (3) 기질(基質)의 건조온도(乾燥溫度)는 높을수록 환원당생성량(還元糖生成量)이 증가(增加)하였으며, 여기서는 건조온도(乾燥溫度)가 $190{\pm}5^{\circ}C$에서 가장 많은 당생성(糖生成)이 나타났다. (4) 기질(基質)의 열처리시간(熱處理時間)이 가장 적당한 것은 45분(分)으로 나타났다. 그리고 45분(分)과 60분간(分間) 열처리(熱處理)한 기질(基質)의 환원당(還元糖) 생성량(生成量)에는 유의차(有意差)가 인정(認定)되지 않았다.

  • PDF

셀로비오스의 미백화장품 소재 가능성 평가를 위한 멜라닌 세포에서 자가포식 및 멜라닌 생성 효능 연구 (Evaluation of the Potential of Cellobiose as a Material for Whitening Cosmetics based on Autophagy and Melanin Production Efficacy in Melanocytes)

  • 차병선;이석주;;정소영;이소민;;이상훈;;빈범호;곽병문;허효진
    • 대한화장품학회지
    • /
    • 제48권4호
    • /
    • pp.365-372
    • /
    • 2022
  • 셀로비오스(cellobiose)는 식물 등에 널리 분포하는 셀롤로오스의 분해 과정에서 생성되는 이당류이다. 환원당인 셀로비오스는 2 개의 β-1,4 결합으로 결합한 구조를 가지며, 식물 등에 존재하는 천연물로서, 건강식품이나 식품의 감미료, 화장품 원료, 의약품 원료로서 활용되어지고 있다. 본 연구에서는 다양하게 유용될 셀로비오스를 기능성 화장품인 미백 화장품 소재로서의 가능성을 확인하고자, 자가포식 활성화 및 멜라닌 생성저해 효능을 검토하였다. 인간 유래 멜라닌 생성 세포로 알려진 MNT-1 세포에서 셀로비오스의 농도에 따른 독성 실험을 진행한 결과 20 mg/mL이하에서 세포독성이 없음을 확인하였고, 공초점 현미경을 통해 셀룰비오스 처리에 따른 자가포식 표지자인 microtubule-associated protein 1 light chain 3 (LC3) puncta의 증가가 확인되었다. 또한 LC3의 형태가 LC3-I에서 LC3-∏로 변환됨을 확인하였으며, 농도와 시간에 따라 LC3-II양이 증가하는 것을 확인하였다. 셀로비오스를 동일 세포에서 20 mg/mL 농도로 7 일간 처리하여 멜라닌 생성량을 분석한 결과, 50% 이상 멜라닌 생성 감소를 확인하였다. 추가적으로, 멜라닌 생성 관련 단백질TYR 및 TYRP1의 발현량을 western blot법을 이용하여 분석한 결과, 농도의존적으로 발현이 저해되어 멜라닌 생성이 감소함을 확인하였다. 이러한 연구 내용을 기반을 토대로 셀로비오스를 함유한 화장품 크림제형을 확보하여 4 주 동안 제형의 변화를 확인 해본 결과 성상이 고온에서 액채형태로 변화하였지만 pH는 변화하지 않았음을 확인하였다. 결론적으로, 자가포식 활성화 및 멜라닌 생성 억제 과정에 셀로비오스가 관여함을 확인하였으며, 이를 통해 미백 화장품 소재로서의 활용 가능성을 제안하고자 한다.

한국(韓國)에 있어서의 장내기생충감염(腸內寄生虫感染)의 현황(現況)과 추이(推移) (Current Status and Transition of the Prevalence of Intestinal Parasitic Infections in Korea)

  • 김동찬
    • 농촌의학ㆍ지역보건
    • /
    • 제9권1호
    • /
    • pp.83-108
    • /
    • 1984
  • Out of a total of 58 species of helminthic and protozoan parasitic infections in Korea, so far 38 species were reported as intestinal parasites of man. Quite a few species of the intestinal parasitic infections have long been prevalent throughout the country and this has been a significant public health problem. In this paper, current status and transition of the intestinal parasitic infections in the past years were presented. Chronological reviewing of data show background and prospects of change in the prevalence of infections. In the national prevalence survey on parasitic infections conducted once every five years since 1971, stool examinations were done by both cellophane thick smear and brine flotation techniques. Every egg positive case of Ascaris lumbricoides and Clonorchis sinensis was further examined by Stoll's dilution egg counting technique. In 1981, perianal swab using adhesive cellulose tape was added for Enterobius infection. For protozoan cyst examination conducted by province and city in '81, fecal specimens were fixed in SAF solution and examined by the formalin-ether concentration technique. High prevalence of parasitic infection in ana before the 1960s can be easily understood from the data given by the Ministry of Health and Social Affairs in 1967. From a parasite control point of view, the 1960s was the preparatory period particularly for control of soil-transmitted helminthiasis. Several organizations which have contributed to parasite control were founded in this period and the prevention law of parasitic infections was passed in '66. In the '70s, overall prevalence rates of the common intestinal parasitic infections, which were highly prevalent in the past were turned into reduction phase for the first time. The '80s can be said to be an active control period of parasitic infections. Intestinal helminths According to the reports of the national prevalence survey on parasitic infections, the prevalence of helminthic infections was 84.3%(number of person examined:24,887) in '71, 63.2%(27,178) in '76, and 41.1%(35,018) in '81. By area, the prevalence rate in '81 was 35.1%(20,569) in urban areas and 49.6%(14,447) in rural areas. Intestinal nematodes Ascaris lumbricoides The prevalence of Ascaris infection has decreased significantly in recent years. Among students, the prevalence was 55.4%, in '69 and decreased to 4.7% in '83. In the national prevalence surveys, the prevalence decreased to 13.0% in '81 from 54.9% in '71. By area, the prevalence decreased to 8.5% in '81 from 46.4% in '71 in urban area and 19.4% in '81 from 59.6% in '71 in rural area. By age, the prevalence has become in recent years relatively even in all age groups, although higher prevalence used to be seen in young age groups of around 10 years old, particulary in the highly prevalent rural areas. By sex, the prevalence was higher in the female than in the male. Unfertilized egg positive rates among the ascariasis cases increased gradually up to 55.4% on the average in '81. The intensity of the infection was also significantly decreased. Trichuris trichiura Trichuris infection had also decreased to 23.4% in '81 from 65.4% in '71. By area, the decreasing tendency of the prevalence became faster in urban areas than in the rural areas. The prevalence in urban and rural areas in '71 was 69.7%, and 63.1% respectively and decreased to 19.5% and 29.0% respectively in '81. By age, the prevalence reached a peak at the 10-14 age group and showed relatively even distribution throughout all age groups. By sex, the prevalence was close in young age groups, but in the 30s or over age group, especially in rural area, the prevalence was significantly higher in the female than in the male. The prevalence has much fluctuated depending in the area. The prevalence in rural areas surveyed in the '80s shows a range between 20.9% and 73.7% by locality. It is anticipated that the prevalence of Trichuris infection will drop more rapidly, when mass treatment is conducted. Hookworms Hookworm infection by mostly Ancylostoma duodenale and a few by Necator americanus has decreased to a negligible levels in recent years. In the national prevalence surveys, the prevalence was 10.7% in '71, 2.2% in '76, and 0.5% in '81. The prevalence was higher in rural areas than in urban areas. Wide application of multi-specific anthelminthics in the ascariasis control programmes conducted in the past decade appear to have been effective against hookworm infection. Trichostrongylus orientalis As in the case with hookworm infection, the prevalence of Trichostrongylus infection has reached a negligible levels. In the national prevalence surveys, the prevalence was 7.7% in '71, 1.0% in '76 and 0.2% in '81. Enterobius vermicularis In the national prevalence survey in '81, the egg positive rate was 12.0%. Higher prevalence is expected when examined repeatedly. The prevalence rate was 10.3% in urban area and 14.6% in rural area. In recent surveys conducted in rural areas among schoolchildren, the prevalence was 32.4% in Gimhae Gun in '82 and 64.1% in Yeongyang Gun in '83. By age, the egg positive rate was higher in young age groups of around 10 and sharply decreased in age groups of around 20 and then somewhat increased again in middle age groups. By sex, the prevalence was higher in the female than in the male. Strongyloides stercoralis Strongyloides stercoralis infection has rarely been found in Korea. Three cases were reported in 1914. And 0.1-0.5% were found infected out of 2,642 persons examined at the prisoner-of-war camp on Geojedo in 1956. One case was reported in '54 and '82, respectively. Anisakis spp. No systematic survey has been conducted for anisakiasis In Korea. So far, only several cases have been found 1 case in Seoul in '71, 5 cases in Busan in '81 and 1 case in Busan in '84. Intestinal trematodes Metagonimus yokogawai In the national prevalence survey conducted in 1981, the egg positive rate was 1.24% on the average. High endemic areas are located in the southwestern part of Korea. The prevalence in Hadong Gun was 29.1% on the average in '79. In a survey conducted in 76, the prevalence was 44.0% in Gwangyang, 55.0% in Gogseung and 29.0% in Gurye. The infection is closely correlated with raw sweetfish consumption in these areas. Other intestinal trematodes A human case of Heterophyes heterophyes was reported in 1914. Several species were reported in the '80s : 17 cases of Fibricola seoulensis, 9 cases of Pygidiopsis summa, 8 cases of Heterophyes heterophyes nocens, 1case of Heterophyopsis continua, 2 cases of Stellantchasmus falcatus, 1 case of Stictodora sp., 1 case of Echinostoma hortense, and 4 cases of Echinochasmus japonicus. As the intermediate hosts, snakes and frogs play a role for F.seoulensis and fish for the rest of the species. Intestinal cestodes Taenia saginata and T. solium Egg positive rates in the national prevalence survey were 0.7% in '76 and 1.1% in '81. The prevalence in '81 was 0.6% in urban area and 1.8% in rural area. The proglottid positive rate in Jeju Do was 19.2% on the average. On Udo, Jeju Do in 1983, the egg positive rate among the inhabitants was 2.9%. Hymenolepis nana In the national prevalence survey, egg positive rates were 0.6% in '76 and 0.4% in '81. No difference was seen in the prevalence by area and sex. Hymenolepis diminuta Infected cases were reported : 3 in '64 and I in '66. Egg positive rate in '81 was 0.01% in the national prevalence survey. Diphylobothrium latum So far, about 30 cases have been reported. The cases have been reported more frequently in recent surveys. Mesocestoides sp. A case was reported from a hospitalized patient in Seoul in '67. Spirometra erinacei Two cases were reported in '84 following reidentification of the adult worms collected in '74. Intestinal protozoa Out of a total of 23 species of human protozoan infections in Korea, 13 species were reported as intestinal protozoa : Entamoeba histolytica, E coli, Endolimax nana, Iodamoeba b$\ddot{u}$tschlii, Dientamoeba fragilis, Giardia lamblia, Chilomastix mesnilii, Embadomonas sp., Enteromonas hominis, Trichomonas hominis, Isospora belli, I. Hominis(Sarcocystis hominis), and Balantidium coli. Since the first report on intestinal protozoan infections in 1925, there have been quite a few survey data on the prevalence of the infection. It was found reviewing the data chronologically that up to the early '70s the infection was prevalent around a 30-50%. After that, the protozoan cyst positive rate has shown the tendency of gradual decrease throughout the country. Protozoan cyst survey conducted in Seoul and several provinces in 1981 revealed infection rates of 8.9%(1,310) in Gangweondo, 10.7%(1,703) in Gyenggi Do, 11.7%(1,032) in Jeonra Buk Do, 9.1%(4,116) in Jeonra Nam Do, and 1.4%(5,275) in Seoul. Entamoeba histolytica In the survey conducted by province in '81, the cyst positive rate was 0.8% in Gangweon-do, 0.3% in Gyeonggi Do, 1.4% in both Jeonra Buk Do and Jeonra Nam Do, and 0.2% in Seoul. Giardia lamblia In the survey by province in '81, cyst positive rates were 2.2% in both Gyeonggi Do and Jeonra Buk Do, 1.9% in Jeonra Nam Do, 0.5% in Gangweon Do, and 0.9% in Seoul. Balantidium coli Two cases were reported. One in 1930 and the other in '74. Isospora belli and I. Hominis(Sarcocystis hominis) Isospora belli was reported : 1 case in '56 and 3 cases in '66. I. Hominis, recently identified to be synonymous with Sarcocystis hominis, was reported : 3 cases in '66. Other intestinal protozoa The protozoan parasites other than the above mentioned are generally treated as commensal, although some of them are considered to be pathogenic. The data of '81 show that about 10% of the inhabitants are still infected with protozoa.

  • PDF

Panellus edulis 수집 균주의 균사배양 및 자실체 특성 분석 (Mycelial Culture and Fruiting Analysis of Panellus edulis Strains Collected in Korea)

  • 우성이;유림;장영선;박영애;정연석;가강현
    • 한국균학회지
    • /
    • 제46권3호
    • /
    • pp.281-294
    • /
    • 2018
  • 국내에서 참부채버섯(Panellus serotinus)으로 수집한 8균주의 internal transcribed spacer 영역 염기서열을 이용하여 계통분석을 실시한 결과 참부채버섯이 아닌 Panellus edulis로 나타났다. Universal Fungal PCR Fingerprinting (UFPF) 분석결과 P. edulis 8균주는 크게 두 그룹으로 나눠졌다. 우리나라 산림에 서식하는 8개 균주의 배양특성을 이해하기 위해 균사의 성장과 효소 활성에 대한 기초특성 연구를 수행했다. 모든 균주는 21일 동안 3종류 배지를 사용하여 배양하였고 생장이 적합한 배지는 malt extract agar (MEA), Sabouraud dextrose agar (SDA) 배지보다 potato dextrose agar (PDA)로, 빠른 균사 생장 및 균사 밀도를 보였다. P. edulis 균주의 균사 생육 조건은 PDA에서는 $20^{\circ}C$, potato dextrose broth (PDB)에서는 $25^{\circ}C$가 선발되었으며, 균사생장에 적합한 수소이온 농도가 PDB는 pH 5~8로 이때 균사생장이 영향을 받지 않았다. 두 균주(NIFoS 2792, 2804)는 PDA의 균사 생장 및 밀도를 기준으로 우수한 균주로 선정되었다. 모든 균주는 셀룰라아제와 라카아제 활성을 나타내었고 NIFoS 2407과 2805는 다른 균주보다 높은 효소 활성을 보였다. 그 중에서도 라카아제 활성은 NIFoS 2387이었고 모든 균주는 셀룰라아제를 생산하였다. 톱밥 종류별 시험에서 상수리나무와 신갈나무에서 균사생장속도가 다른 톱밥에서보다 좋았고 균사 밀도도 가장 양호하였다. 버섯의 톱밥재배는 $23^{\circ}C$에서 균 접종 후 80~85일간 배양하였고, 원기형성 온도는 $17{\sim}18^{\circ}C$에서 10~11일간, 자실체 생육은 15~20일간 수행하였다. 버섯 재배 결과, 배양기간과 수량 면에서 NIFoS 2804, NIFoS 3993 균주가 가장 양호한 균주로 선발되었다. 이러한 결과는 추후 P. edulis의 인공재배를 하는데 유용한 정보로 활용 될 수 있을 것이다.