• 제목/요약/키워드: Cellulase treatment

검색결과 215건 처리시간 0.025초

텐셀소재의 fibrillation에 관한 연구(제I보) -셀룰라제로 처리한 텐셀의 물성변화를 중심으로- (A Study on Fibrillation of Tencel Material(Part I) -Based on Change of Properties of Tencel by Cellulase-)

  • 정영희;송경헌;양진숙
    • 한국의류학회지
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    • 제25권3호
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    • pp.507-515
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    • 2001
  • Tencel is a high quality cellulosic fiber, which is controlled and processed by an environmentally sound route. But, the point about tencel and the reason why it achieves its unique touch in its finished state is that it is a fibrillating fiber. That means it can take a great deal of punishment during the finishing process, which raise the fiber to produce the characteristics handle, without destroying the cloth. The aim of cellulase treatment is to improve appearence of tencel, plus the fiber ends protruding from the fabric surface. But enzymatic hydrolysis can weakens the fiber ends and changes the properties of fabrics. This study examined about the changes of properties according to several conditions and effect of cellulase to fibril of tencel. The results are as follows. The weight loss was occurred, tensile strength was decreased, softness was increased. And cellulase treatment reduced amount of fibril.

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마직물의 셀룰라이제 효소처리에 의한 유연가공효과에 관한 연구 (The Effect of Biopolishing with Cellulase Enzyme on Ramie and Hemp Fabrics)

  • 김정희;유혜자
    • 한국의류산업학회지
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    • 제3권4호
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    • pp.367-372
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    • 2001
  • Five kinds of commercial ramie and hemp fabrics were treated with cellulase under different concentrations. Samples were mercerized before enzyme treatment to investigate the effect of mercerization on cellulase enzyme treatment. Physical characteristics(weight loss, tear strength retention, wrinkle recovery, drape stiffness, dyeability) of cellulase enzyme treated and untreated samples were measured and compared. X-ray diffractions were examined to verify if there were any changes in their crystallinity of enzyme treated fabrics. Weight loss, wrinkle recovery and degree of crystallinity increased as the concentration of cellulase enzyme increased. In the meanwhile, tear strength retention and drape stiffness and dyeability decreased. Enzyme activity was more effective on mercerized samples. Particularly, there was distinct tendency to increase weight loss and flexibility.

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산성 셀룰라제 이용한 데님의 효소가공 및 물성 (Enzymatic Processing and Property of Denim by Acid Cellulase)

  • 서혜영;송화순;김혜림
    • 한국의류산업학회지
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    • 제11권3호
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    • pp.465-468
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    • 2009
  • In this study, acid cellulase was used to treat denim fabrics by varying pH, temperature, enzyme concentration, treatment time and non-ionic surfactant (Triton X-100) concentration. Treatment condition was controlled based on the weight loss. The characteristics of enzyme-treated fabrics were measured in terms of tearing strength, stiffness, and color difference. The optimum conditions for cellulase treatment of denim fabric were pH 5.0, $50^{\circ}C$, 3% (o.w.f.), 90minutes. The weight loss did not change significantly with the addition of a non-ionic surfactant, but it improved when more non-ionic surfactant were used. The tearing strength of enzyme-treated denim fabrics did not deteriorate. The stiffness of the treated fabrics improved with the enzymatic treatment with and without the non-ionic surfactant. The difference in color of fabrics treated with enzyme increased.

효소 처리 홍삼을 함유한 오일의 항산화 효과 (Antioxidant Effect of Oil Containing Cellulase-Treated Red Ginseng.)

  • 김현정;양선아;임남경;지광환;이인선
    • 생명과학회지
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    • 제18권3호
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    • pp.323-328
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    • 2008
  • 홍삼의 유효 활성 성분을 함유한 오일을 제조하고자, 세절한 홍삼을 여러 효소로 처리하여 항산화능을 검토한 후 가장 적합한 효소를 선정하여 홍삼을 효소처리하고 10배 비율의 대두유 및 올리브유를 각각 첨가하여 $40^{\circ}C$ 에서 15일간 숙성하면서 홍삼유를 제조하였다. 그 결과, 0.5% cellulase로 처리한 홍삼액에서 가장 높은 항산화 활성이 있음을 확인하였다. 그리고 홍삼을 0.5% cellulase로 처리하여 제조한 홍삼유의 과산화물가는 오일 자체만 보관한 대조군에 비해 유의적으로 감소되었고, 홍삼의 항산화 활성 성분의 용출은 올리브유보다 대두유에서 더 증가하였고, 특히 0.5% cellulase 처리한 홍삼 대두유의 TBA가도 유의적으로 감소되었다. 홍삼유의 DPPH radical 소거능도 홍삼만으로 추출한 대두유에 비해 0.5% cellulase 처리한 홍삼 대두유에서 더 큰 DPPH 소거능을 보였다. 대두유로 제조한 홍삼유의 항산화비타민 함량 분석에서는 비타민 A는 검출되지 않았으나, 비타민 E 함량은 대두유보다 홍삼 첨가 대두유에서 1.7배 정도 증가하였고, 0.5% cellulase를 처리한 홍삼첨가 대두유에서 2.3배 정도 증가되어 홍삼의 0.5% cellulase 처리에 의해 비타민 E의 용출이 더 증가하였다.

목재부후균, Acanthophysium sp. KMF001, 유래 섬유소분해효소를 이용한 섬유 개량 (Biopolishing of Cotton Fabric using Crude Cellulases from Acanthophysium sp. KMF001)

  • 신금;윤새민;김주혜;김영균;김태종;김영숙
    • Journal of the Korean Wood Science and Technology
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    • 제44권3호
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    • pp.381-388
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    • 2016
  • 면직물 표면의 잔털을 제거하여 새 옷 같은 느낌을 주기 위한 환경친화적 방법으로 셀룰라아제를 이용한 바이오폴리싱 방법이 도입되어 산업적으로 널리 이용되고 있다. 본 연구에서는 목재의 당화능력이 우수한 Acanthophysium sp. KMF001 효소를 분리해내어 이 효소의 바이오폴리싱 효과를 평가하였다. 실험을 통해 선정한 최적 바이오폴리싱 처리조건은 $50^{\circ}C$, pH 4.5, 처리효소농도는 면직물 중량 대비 10%, 반응시간은 60분으로 나타났다. 실험결과 최적화된 조건에서 처리한 면직물의 인장강도는 감소율이 상업용 효소보다 적었으며 전자현미경 관찰결과 잔털제거효과가 우수하여 바이오폴리싱 효과가 있는 것으로 평가되었다. 따라서 실험에 사용한 Acanthophysium sp. KMF001이 바이오폴리싱에 효과적으로 적용 가능한 효소로 판단된다.

중성 셀룰라제 처리에 의한 데님의 물성 (Effect of Cellulase on Characteristics of Denim)

  • 김지연;송화순;김혜림
    • 한국의류산업학회지
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    • 제11권3호
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    • pp.469-473
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    • 2009
  • Recently, eco-friendly processing has been focused in the textile industry in order to reduce environmental pollutions. Applications of enzyme technology to the textile industry are an example of more environmentally compatible processes. However, there is not enough quantity of referring to denim fabric subjected to enzymatic treatment. In this study, depending on pH, temperature, cellulase concentration, and treatment time, the weight loss of denim fabrics was examined. Characteristics of enzyme-treated fabrics were measured by tearing strength, stiffness, and K/S values. The effect of a non-ionic surfactant (Triton X-100) on characteristics of the enzyme-treated fabrics was evaluated. The cellulase treatment condition on the cotton fabric were optimized to pH 6.0, $50^{\circ}C$, 1%(o.w.f.), and 60minutes. Characteristics of denim fabrics by cellulase treatment in the presence of Triton X-100 did not improve because Triton-X inhibited the activity of enzyme.

효소와 미생물의 복합 처리에 의한 두유박 단백질소재의 제조 (Production of a Protein Supplement from Soymilk Residues by Combined Use of Enzymes and Microorganisms)

  • 채희정;이만진;이종대
    • Applied Biological Chemistry
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    • 제41권1호
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    • pp.73-77
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    • 1998
  • 두유박의 단백질 소재화를 위하여 셀룰레이즈(cellulase), 단백질 분해효소, 코지(koji), 효모 등의 복합처리에 의한 가용화 방법이 고형분 및 단백질 회수율, 아미노산, 유기산 조성에 미치는 영향을 고찰하였다. 셀룰레이즈와 단백질 분해효소를 복합 사용하여 두유박을 가수분해하는 것이 높은 고형분 회수율(80.2%) 및 단백질회수율(83.5%)을 얻을 수 있었고, 코지와 효모로 처리할 경우 유기산의 함량을 증대시킬 수 있었으며 양조간장 특유의 맛과 향을 내는데 효과적이었다. 특히 셀룰레이즈로 처리한 가수분해물에서 유기산 함량이 높았다. 유리 아미노산 함량은 코지보다 단백질 분해효소로 분해한 경우에서 높았으며 셀룰레이즈 처리는 유리 아미노산 함량에 큰 영향을 미치지 않았다. 두유박 가수분해물에 대한 관능검사 결과 코지로 가수분해한 시료가 관능적으로 우수하였으나 전체적인 기호도는 대조구로 사용된 양조간장에 비해 낮았다.

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효소처리에 의한 블루베리 음료 생산을 위한 최적추출조건 (Optimization of blue berry extraction for beverage production using enzyme treatment)

  • 지유정;임무혁
    • 한국식품저장유통학회지
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    • 제24권1호
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    • pp.60-67
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    • 2017
  • 본 연구에서는 저온추출 뿐만 아니라 효소처리를 이용하여 생과형 블루베리 과일음료생산을 위한 최적의 방법을 확립하고자 하였다. 생리학적 기능성물질 추출 시 열에 의한 영양손실을 막기 위해 저온을 사용하였다. 또한 다양한 효소처리를 이용하여 혼탁 방지 및 추출수율 향상을 위한 최적의 추출조건을 확립하고자 하였다. 블루베리를 cellulase, pectinase 및 cellulase:pectinase(1:1) 혼합 효소와 효소 처리량, 추출온도, 추출시간, 추출 교반속도 등을 고려한 다양한 조건으로 추출하였을 때, 가장 우수한 추출 조건을 조사하였다. cellulase로 처리할 경우, 추출률이 85.72-86.55%, pectinase 처리구는 87.06-87.93%, cellulase:pectinase(1:1) 혼합 처리할 경우, 86.84-88.14%의 추출률을 나타냈다. 추출 온도는 $45^{\circ}C$에서 $87.91{\pm}0.05%$, 3시간 추출 하였을 경우 $87.88{\pm}0.10%$, 교반속도는 90 rpm에서 가장 높은 추출결과를 보였다. 블루베리 추출액의 당도 및 pH는 추출 조건에 관계없이 모든 처리구에서 통계학적으로 유사한 결과를 보였으나, 총 폴리페놀 함량은 pectinase 처리구에서 18.62 mg/g으로 가장 높은 값을 나타냈다. 유리당 함량은 모든 시료에서 과당과 포도당 두 가지 당류만 검출되었으며 cellulase 효소 0.10%를 처리한 블루베리 추출액의 유리당 함량이 포도당 0.51%, 과당 0.49%로 가장 높았으나 다른 처리구와 유의적인 차이는 없었다. 블루베리를 이용하여 생과일형 음료 제조를 위한 최적 조건은 cellulase: pectinase(1:1) 혼합효소 0.1%를 첨가하여 $45^{\circ}C$ 온도에서 90 rpm의 교반속도로 조사되었다.

셀룰라제처리에 의한 섬유소계직물의 감량률과 물리적성능 변화 (Weight Loss Rates and Physical Properties Changes of Cellulose Fabrics by Cellulase Treatment)

  • 이혜자;전혜경;유혜자
    • 대한가정학회지
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    • 제37권12호
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    • pp.169-177
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    • 1999
  • This study has examined weight loss rates of Iyoceu, lyocell/cotton, cotton that were treated with cellulase under different concentration, time, temperature and pH. and compared physical properties changes of tensile strength, drape, moisture absorbency, shrinkage and dyeability. The notable results are summarized as follows: Lyocell was in need of pretreatment by NaOH in the side of weight loss, tensile strength and dyeability. Weight loss rates of cellulose fabrics by cellulase treatment were in the order of cotton > lyocell/cotton > lyocell at the same conditions. In case of lyocell and lyocell/cotton, weight loss rates showed up lower than cotton, while strength retention decreased, drape and strength flexibility were highly improved after cellulase treatment.

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Effects of Combined Treatment of Lactic Acid Bacteria and Cell Wall Degrading Enzymes on Fermentation and Composition of Rhodesgrass (Chloris gayana Kunth.) Silage

  • Ridla, M.;Uchida, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제11권5호
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    • pp.522-529
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    • 1998
  • This experiment was conducted to study the effects of lactic acid bacteria (LAB) inoculation either alone or in combination with cell wall degrading enzymes on the fermentation characteristics and chemical compositions of Rhodesgrass silage. Over to 1 kg of fresh Rhodesgrass sample a treatment of inoculant LAB with or without addition of an enzyme of Acremoniumcellulase (A) or Meicelase (M) or a mixture of both enzymes (AM) was applied. The treatments were control untreated, LAB-treated (application rate $1.0{\times}10^5cfu/g$ fresh sample), LAB+A 0.005%, LAB+A 0.01%, LAB+A 0.02%, LAB+M 0.005%, LAB+M 0.01%, LAB+M 0.02 %, LAB+AM 0.005%, LAB+AM 0.01%, and LAB+AM 0.02%. The sample was ensiled into 2-L vinyl bottle silo, with 9 silages of each treatment were made. Three silages of each treatment were incubated at 20, 30 and $40^{\circ}C$ for 2-months of storage period. All silages were well preserved with their fermentation quality has low pH values (3.91-4.26) and high lactic acid concentrations (4.11-9.89 %DM). No differences were found in fermentation quality and chemical composition of the control untreated silage as compared to the LAB-treated silage. Combined treatment of LAB+cellulases improved the fermentation quality of silages measured in terms of lower (p < 0.01) pH values and higher (p < 0.05) lactic concentrations than those of LAB-treated silages. Increasing amount of cellulase addition resulted in decrease (p < 0.05) of pH value and increase (p < 0.05) of lactic acid concentration. LAB + cellulase treatments (all cellulase types) reduced (p < 0.01) NDF, ADF and in vitro dry matter digestibility of silages compared with the control untreated silages. The fermentation quality and the rate of cell wall reduction were higher (p < 0.01) in the silages treated with LAB + cellulase A than in the silages treated with either LAB+cellulase M or LAB + cellulase AM. Incubation temperature of $40^{\circ}C$ was likely to be more appropriate environment for stimulating the fermentation of Rhodesgrass silages than those of 20 and $30^{\circ}C$.