• Title/Summary/Keyword: Cell-in-cell

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Stem Cell Biotechnology for Cell Therapy

  • LEE Dong-Ree;KIM Ha Won
    • Biomolecules & Therapeutics
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    • v.13 no.4
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    • pp.199-206
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    • 2005
  • Cell therapy (CT) is a group of techniques to treat human disorders by transplantation of cells which have been processed and propagated independent of the living body. Blood transfusion and bone marrow transplant have been the primary examples of cell therapy. With introduction of stem cell (SC) technologies, however, CT is perceived as the next generation of biologies to treat human diseases such as cancer, neurological diseases, and heart disease. Despite potential of cell therapy, insufficient guidelines have been implemented concerning safety test and regulation of cell therapy. This review addresses the safety issues to be resolved for the cell therapy, especially SC therapy, to be successfully utilized for clinical practice. Adequate donor cell screening must preceed to ensure safety in cell therapy. In terms of SC culture, controlled, standardized practices and procedures should be established. Further molecular studies should be done on SC development and differentiation to enhance safety level in cell therapy. Finally, animal model must be further installed to evaluate toxicity, new concepts, and proliferative potential of SC including alternative feeder layer of animal cells.

A 3-cell CCI(Cell-to-Cell Interference) model and error correction algorithm for Multi-level cell NAND Flash Memories (다중셀 낸드 플래시 메모리의 3셀 CCI 모델과 이를 이용한 에러 정정 알고리듬)

  • Jung, Jin-Ho;Kim, Shi-Ho
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.48 no.10
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    • pp.25-32
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    • 2011
  • We have analyzed adjacent cell dependency of threshold voltage shift caused by the cell to cell interference, and we proposed a 3-adjacent-cell model to model the pattern dependency of the threshold voltage shift. The proposed algorithm is verified by using MATLAB simulation and measurement results. In the experimental results, we found that accuracy of the proposed simple 3-adjacient-cell model is comparable to the widely used conventional 8-adjacient-cell model. The Bit Error Rate (BER) of LSB and of MSB is improved by 28.9% and 19.8%, respectively, by applying the proposed algorithm based on 3-adjacent-cell model to 20nm-class 2-bit MLC NAND flash memories.

Reconstruction of Receptive Field of Retinal Ganglion Cell Using Matlab (Matlab을 이용한 망막신경절세포 감수야 구성)

  • Ye, Jang-Hee;Jin, Gye-Hwan;Goo, Yong-Sook
    • Progress in Medical Physics
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    • v.17 no.4
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    • pp.260-267
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    • 2006
  • A retinal ganglion cell's receptive field is defined as that region on the retinal surface In which a light stimulus will produce a response. A retinal ganglion cell peers out at a small patch of the visual scene through its receptive field and encodes local features with action potentials that pass through the optic nerve to higher centers. Therefore, defining the receptive field of a retinal ganglion cell is essential to understand the electrical characteristics of a ganglion cell. Distribution of receptive fields over retinal surface provides us an Insight how the retinal ganglion cell processes the visual scene. In this paper, we provide the details how to reconstruct the receptive field of a retinal ganglion cell. We recorded the ganglion cell's action potential with multielectrode array when the random checkerboard stimulus was applied. After classifying the retinal waveform Into ON-cell, OFF-cell, ON/OFF-cell, we reconstructed the receptive field of retinal ganglion cell with Matlab. Here, we show the receptive fields of ON-cell and OFF-cell.

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Investigating the role of Sirtuins in cell reprogramming

  • Shin, Jaein;Kim, Junyeop;Park, Hanseul;Kim, Jongpil
    • BMB Reports
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    • v.51 no.10
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    • pp.500-507
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    • 2018
  • Cell reprogramming has been considered a powerful technique in the regenerative medicine field. In addition to diverse its strengths, cell reprogramming technology also has several drawbacks generated during the process of reprogramming. Telomere shortening caused by the cell reprogramming process impedes the efficiency of cell reprogramming. Transcription factors used for reprogramming alter genomic contents and result in genetic mutations. Additionally, defective mitochondria functioning such as excessive mitochondrial fission leads to the limitation of pluripotency and ultimately reduces the efficiency of reprogramming. These problems including genomic instability and impaired mitochondrial dynamics should be resolved to apply cell reprograming in clinical research and to address efficiency and safety concerns. Sirtuin (NAD+-dependent histone deacetylase) has been known to control the chromatin state of the telomere and influence mitochondria function in cells. Recently, several studies reported that Sirtuins could control for genomic instability in cell reprogramming. Here, we review recent findings regarding the role of Sirtuins in cell reprogramming. And we propose that the manipulation of Sirtuins may improve defects that result from the steps of cell reprogramming.

Study on In Vitro Development of Mouse Embryos (생쥐 수정란의 핵이식후 체외발달에 관한 연구)

  • 박희성;이효종;최상용;박충생
    • Korean Journal of Animal Reproduction
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    • v.14 no.3
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    • pp.205-211
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    • 1990
  • Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell mouse embryos by micromanipulation and sendai virum mediated fusion. no significant difference in successful injectin rate and fusion rate was found between the cell stages of nuclear donor embryos. There nuclear transplant embryos receiving different cell stage nuclei were cultured in vitro for 96 hours. 75.3% of 255 embryos receiving 2-cell nuclei, 68.2% of 236 embryos reciving 4-cell nuceli and 46.9% of 228 embryos receiving 8-cell nuclei were developed to blastocyst, respectively. The number of blastomeres was significantly(P<0.05) reduced in the embryos receiving 8-cell nuclei, compared with the embryos receiving 2-cell, 4-cell nuclei or the intact embryos. Also the size of blastocysts was significantly(P<0.05) smaller in the embryos receiving 8-cell nuclei, compared with the intact or other nuclear transplant embryos. These results suggest that single nuclei introduced into the enucleated two-cell embryos are able to support the in vitro development of the reconstituted embryos to blastocysts. The prominant retardation of blastocoele formation and cell division was shown in nuclear transplant embryos receiving eight-cell nuclei when they were cultured in vitro.

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Protective Effect of Bcl-2 in NS0 Myeloma Cell Culture is Greater in More Stressful Environments

  • Tey, B.T.;Al-Rubeai, M.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.564-570
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    • 2005
  • In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%. The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis. For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important in the suspension culture. When the NS0 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic.

Natural killer T cell and pathophysiology of asthma

  • Jang, Gwang Cheon
    • Clinical and Experimental Pediatrics
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    • v.53 no.2
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    • pp.136-145
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    • 2010
  • Natural killer T (NKT) cell is a special type of T lymphocytes that has both receptor of natural killer (NK) cell (NK1.1, CD161c) and T cell (TCR) and express a conserved or invariant T cell receptor called $V{\alpha}14J{\alpha}18$ in mice or Va24 in humans. Invariant NKT (iNKT) cell recognizes lipid antigen presented by CD1d molecules. Marine-sponge-derived glycolipid, ${\alpha}-galactosylceremide$ (${\alpha}-GalCer$), binds CD1d at the cell surface of antigen-presenting cells and is presented to iNKT cells. Within hours, iNKT cells become activated and start to secrete Interleukin-4 and $interferon-{\gamma}$. NKT cell prevents autoimmune diseases, such as type 1 diabetes, experimental allergic encephalomyelitis, systemic lupus erythematous, inflammatory colitis, and Graves' thyroiditis, by activation with ${\alpha}-GalCer$. In addition, NKT cell is associated with infectious diseases by mycobacteria, leshmania, and virus. Moreover NKT cell is associated with asthma, especially CD4+ iNKT cells. In this review, I will discuss the characteristics of NKT cell and the association with inflammatory diseases, especially asthma.

Microfluidic Control for Biological Cell Orientation

  • Namkung, Young-Woo;Park, Jung-Yul;Kim, Byung-Kyu;Park, Jong-Oh;Kim, Jin-Oh
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2457-2460
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    • 2003
  • There is a great demand to manipulate biological cell autonomously since biologist should spend much time to obtain skillful manipulation techniques. For this purpose, we propose a cell chip to control, carry, fix and locate the cell. In this paper, we focus on the cell rotator to rotate individual biological cell based on a micro fluidics technology. The cell rotator consists of injection hole and rotation well to rotate a biological cell properly. Under the variation of flow rate in injection hole, the angular velocity of a biological cell is evaluated to find the feasibility of the proposed rotation method. As a practical experiment, Zebrafish egg is employed. Based on this research, we find the possibility of non-contact rotation way that can highly reduce the damage of the biological cell during manipulation. To realize an autonomous biological cell manipulation, a cell chip with manipulation well and micro channel in this research will be utilized effectively in near future.

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Modulatory Effect of BAY11-7082 on CD29-mediated Cell-cell Adhesion in Monocytic U937 Cells (BAY11-7082에 의한 U937 세포의 CD29-매개성 세포간 유착과정 조절 효과)

  • Kim, Byung-Hun;Cho, Jae-Youl
    • YAKHAK HOEJI
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    • v.52 no.5
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    • pp.412-417
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    • 2008
  • BAY11-7082 was initially found to be an anti-inflammatory drug with NF-${\kappa}B$ inhibitory property. In this study, we evaluated modulatory function of BAY11-7082 on U937 cell-cell adhesion induced by CD29 (${\beta}1$-integrins). BAY11-7082 strongly blocked functional activation of CD29 (${\beta}1$-integrins), as assessed by cell-cell adhesion assay. However, this compound did not block a simple activation of CD29, as assessed by cell-fibronectin adhesion assay. In particular, to understand molecular mechanism of BAY11-7082-mediated inhibition, the regulatory roles of CD29-induced actin cytoskeleton rearrangement under cell-cell adhesion and surface level of CD29 were examined using confocal and flow cytometic analysis. Interestingly, this compound strongly suppressed the molecular association of actin cytoskeleton with CD29 at cell-cell adhesion site. Moreover, BAY11-7082 also diminished surface levels of CD29 as well as its-associated adhesion molecule CD147, but not other adhesion molecules such as CD18 and CD43. Therefore, our data suggest that BAY11-7082 may be involved in regulating immune responses managed by CD29-mediated cell-cell adhesion.

Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro

  • Park, Yun-Gwi;Lee, Seung-Eun;Kim, Eun-Young;Hyun, Hyuk;Shin, Min-Young;Son, Yeo-Jin;Kim, Su-Young;Park, Se-Pill
    • Development and Reproduction
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    • v.19 no.3
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    • pp.119-126
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    • 2015
  • The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO) compared to feeder free condition (D3/-) as a control group. Although there were no differences to colony formations and AP activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/- (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF ($72.8{\pm}7.69$ and $81.2{\pm}3.56$) than D3/STO ($32.0{\pm}4.30$ and $56.0{\pm}4.90$) or D3/- ($55.0{\pm}4.64$ and $62.0{\pm}6.20$). These results suggest that MEF feeder cell layer is more suitable to mES cell culture.