• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.7A
• /
• pp.620-627
• /
• 2005

We develop an adaptive hot-spot operating scheme(AHOS) to mitigate the negative effects from the nonuniform distribution of user location and the variation in the mixture of QoS requirements in OFDMA downlink systems. The base station in a macrocell can control the operation of picocells within the cell, and turns on or off according to the changes in the estimated user outage probability and the AHOS gain parameter. With the computer simulation, the AHOS has been proved to maximize the system throughput while maintaining the QoS outage probability very low under various system scenarios.

• Biomedical Science Letters
• /
• v.16 no.4
• /
• pp.279-285
• /
• 2010

Endogenous cannabinoids (endocannabinoids) display various pharmacological effects including pain control, anti-inflammation, and neuroprotection. The synthesis and release of endocannabinoids are regulated under both physiological and pathological conditions. The main degrading enzyme of endocannabinoid is fatty acid amide hydrolase (FAAH). Therefore we have developed the fluorescence-based assay system for FAAH. We established stable CosM6 cell lines expressing human FAAH. We also synthesized 2-oxo-2H-chromen-7-yl decanoate (DAEC) as a fluorogenic substrate for FAAH. When crude membrane extracts stably expressing FAAH was incubated with DAEC at $25^{\circ}C$, FAAH reacted specifically to DAEC and catalyzes the hydrolysis of DAEC into decanoic acid and highly fluorescent coumarin. Furthermore, the serin hydrolase inhibitor, phenylmethanesulfonylfluoride, inhibited the coumarin release to the reaction buffer in concentration dependent manner. This assay system is suitable for high-throughput screening since this system has simple experimental procedure and measurement method.

• The Plant Pathology Journal
• /
• v.24 no.1
• /
• pp.24-30
• /
• 2008

Colletotrichum gloeosporioides forms an appressorium, a specialized infection structure, to infect its hosts. Among 400 and 600 culture filtrates from fungi and class Actinomycetes, six methanol extracts (A5005, A5314, A5387, A5560, A5597, and A5598) from the class Actinomycetes significantly inhibited appressorium formation in C. gloeosporioides infecting pepper fruits in a dose-dependent manner, while conidial germination was slightly enhanced. Two (A5005 and A5560) of them also exhibited distinctive inhibitory effect on the disease progress of pepper anthracnose. Water fractions of both culture filtrates also specifically inhibited appressorium formation in C. gloeosporioides and pepper anthracnose disease. Inhibition of appressorium formation by culture filtrate of A5005 was partially restored by the exogenous calcium. This results suggests that chemicals within A5005 extents its biological activity through disturbance of intracellular $Ca^{2+}$ regulation during prepenetration morphogenesis by C. gloeosporioides. Together, cell-based and target-oriented screening system used in this study should be applicable for other plant pathogenic fungi prerequisite appressorium formation to infect their hosts.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2002.04b
• /
• pp.19-25
• /
• 2002

Biotechnology in the 21st century will be driven by three emerging technologies: genomics, high-throughput biology, and bioinformatics. These technologies are complementary to one another. A large number of economically important crops are currently subjected to whole genome sequencing. Functional genomics for determining the functions of the genes comprising the given plant genome is under progress by using various means including phenotyping data from transgenic mutants, gene expression profiling data from DNA microarrays, and metabolic profiling data from LC/mass analysis. The aim of plant molecular breeding is shifting from introducing agronomic traits such as herbicide and insect resistance to introducing quality traits such as healthful oils and proteins, which will lead to improved and nutritional food and feed products. Plant molecular breeding is also expected to aim to develop crops for producing human therapeutic and industrial proteins.

• BMB Reports
• /
• v.35 no.3
• /
• pp.262-266
• /
• 2002

A non-isotopic neutravidin-based reverse transcriptase (RT) assay adapted for high throughput screening of HIV activity is described. Using a 96-well microtitre plate, HIV particles are lysed and the RT enzyme released into a reaction mixture containing poly(A) RNA, biotinylated oligo d(T) and fluorescein-labelled dUTP (FI-dUTP). With poly(A) as a template and oligo d(T) as primer, the viron RT incorporates FI-dUTP into an elongating DNA strand. The resulting product is captured on a neutravidin-coated 96-well plate and the unincorporated nucleotides removed by a series of washing steps. A simple ELISA is subsequently performed using a monoclonal antifluorescein antibody conjugated to alkaline phosphatase. Quantification of RT activity is facilitated by a colorimetric readout. The assay was validated in the context of a diagnostic HIV-1 phenotyping assay. Using supernatants from HIV-1 infected lymphocyte cultures the assay was shown to be as sensitive as a radioactive assay and the RT activity correlated well with levels of cell-asociated HIV-p24. Importantly, even minor reductions of RT activity by virus variants with reduced fitness could be distinguished.

• Molecules and Cells
• /
• v.23 no.2
• /
• pp.170-174
• /
• 2007

High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.11 no.11
• /
• pp.5264-5281
• /
• 2017

Multihop relay-based cellular networks are attracting much interest because of their throughput enhancement, coverage extension, and low infrastructure cost. In these networks, relay stations (RSs) between a base station (BS) and mobile stations (MSs) drastically increase the overall spectral efficiency, with improved channel quality for MSs located at the cell edge or in shadow areas, and enhanced throughput of MSs in hot spots. These relay-based networks require an advanced radio resource management scheme because the optimal amount of radio resource for a BS-to-RS link should be allocated according to the MS channel quality and distribution, considering the interference among RSs and neighbor BSs. In this paper, we propose optimal resource planning algorithms that maximize the overall utility of relay-based networks under a proportional fair scheduling policy. In the first phase, we determine an optimal scheduling policy for distributing BS-to-RS link resources to RSs. In the second phase, we determine the optimal amount of the BS-to-RS link resources using the results of the first phase. The proposed algorithms efficiently calculate the optimal amount of resource without exhaustive searches, and their accuracy is verified by comparison with simulation results, in which the algorithms show a perfect match with simulations.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.25 no.1A
• /
• pp.56-63
• /
• 2000

In this paper, we design new high performance ATM switch architectures based on a Generalized Shuffle network(GSN). The GSN is a distributed network topology with the number of nodes in O(N). To improve the throughput of the switch, a layering strategy called Weaved GSN(WGSN). WGSN has an additional connection links between switching elements which locate in the same position of adjacent GSNs. The analysis and simulation are performed under uniform and full load conditions, and the results show that the proposed switch has better throughput and cell loss performance when compared with other banyan-based switch architectures known so far.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.3
• /
• pp.329-334
• /
• 2013

Uridinediphospho-N-acetylglucosamine enolpyruvyl transferase (MurA, E.C. 2.5.1.7) is an essential bacterial enzyme that catalyzes the first step of the cell wall biosynthetic pathway, which involves the transfer of an enolpyruvyl group from phosphoenolpyruvate to uridinediphospho-Nacetylglucosamine. In this study, novel inhibitors of Haemophilus influenzae MurA (Hi MurA) were identified using high-throughput screening of a chemical library from the Korea Chemical Bank. The identified compounds contain a quinoline moiety and have much lower effective inhibitory concentrations ($IC_{50}$) than fosfomycin, a wellknown inhibitor of MurA. These inhibitors appear to covalently modify the sulfhydryl group of the active site cysteine (C117), since the C117D mutant Hi MurA was not inhibited by these compounds and excess dithiothreitol abolished their inhibitory activities. The increased mass value of Hi MurA after treatment with the identified inhibitor further confirmed that the active-site cysteine residue of Hi MurA is covalently modified by the inhibitor.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.28 no.12A
• /
• pp.958-963
• /
• 2003

Increasing throughput using high order data modulations in the WCDMA downlink requires the elimination of multipath interferences. For this reason, Kenichi Higuchi proposed a multipath interference canceller in WCDMA downlink. However, this paper considers the downlink signal models that only contains code channels for which code information is known to the receiver. WCDMA systems contain code channels for other active users whose code information is unknown to the receiver and these code channels degrade the performance of the multipath interference canceller. Thus, this paper proposes a multipath interference mitigation scheme which removes multipath interference induced by all the code channels within a cell. Simulation results show that the proposed scheme outperforms both the RAKE receiver and the multipath interference canceller in the WCDMA downlink.