• Journal of the Institute of Convergence Signal Processing
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• v.12 no.2
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• pp.83-90
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• 2011

In cell biology area, microscopy enables detecting objects inside cells that are stained or fluorescently tagged. It is disadvantageous for observing these objects because of the noisy characteristics of their environmental surrounding. In this paper, a framework is proposed to increase the throughput and reliability for analysis of these images. First, we apply adaptive beamlet transform to extract edges meaningfully followed by orientation, location, and length in different scales. Then, a post-process is implemented to extend and map them onto original image. Our proposed scheme is compared with Canny edge detector and conventional beamlet transform from four evaluation aspects. It produces better results when experiments are conducted on real images. Much better results for observing internal parts make this framework competitive for analysis of cell images.

• Journal of Pharmaceutical Investigation
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• v.30 no.3
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• pp.191-199
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• 2000

Genomics is providing targets faster than we can validate them and combinatorial chemistry is providing new chemical entities faster than we can screen them. Historically, the drug discovery cascade has been established as a sequential process initiated with a potency screening against a selected biological target. In this sequential process, pharmacokinetics was often regarded as a low-throughput activity. Typically, limited pharmacokinetics studies would be conducted prior to acceptance of a compound for safety evaluation and, as a result, compounds often failed to reach a clinical testing due to unfavorable pharmacokinetic characteristics. A new paradigm in drug discovery has emerged in which the entire sample collection is rapidly screened using robotized high-throughput assays at the outset of the program. Higher-throughput pharmacokinetics (HTPK) is being achieved through introduction of new techniques, including automation for sample preparation and new experimental approaches. A number of in vitro and in vivo methods are being developed for the HTPK. In vitro studies, in which many cell lines are used to screen absorption and metabolism, are generally faster than in vivo screening, and, in this sense, in vitro screening is often considered as a real HTPK. Despite the elegance of the in vitro models, however, in vivo screenings are always essential for the final confirmation. Among these in vivo methods, cassette dosing technique, is believed the methods that is applicable in the screening of pharmacokinetics of many compounds at a time. The widespread use of liquid chromatography (LC) interfaced to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) allowed the feasibility of the cassette dosing technique. Another approach to increase the throughput of in vivo screening of pharmacokinetics is to reduce the number of sample analysis. Two common approaches are used for this purpose. First, samples from identical study designs but that contain different drug candidate can be pooled to produce single set of samples, thus, reducing sample to be analyzed. Second, for a single test compound, serial plasma samples can be pooled to produce a single composite sample for analysis. In this review, we validated the issue whether the second method can be applied to practical screening of in vivo pharmacokinetics using data from seven of our previous bioequivalence studies. For a given drug, equally spaced serial plasma samples were pooled to achieve a 'Pooled Concentration' for the drug. An area under the plasma drug concentration-time curve (AUC) was then calculated theoretically using the pooled concentration and the predicted AUC value was statistically compared with the traditionally calculated AUC value. The comparison revealed that the sample pooling method generated reasonably accurate AUC values when compared with those obtained by the traditional approach. It is especially noteworthy that the accuracy was obtained by the analysis of only one sample instead of analyses of a number of samples that necessitates a significant man-power and time. Thus, we propose the sample pooling method as an alternative to in vivo pharmacokinetic approach in the selection potential lead(s) from combinatorial libraries.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.4
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• pp.794-799
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• 2015

To manage cell outage problem in indoor wireless communication systems, we should resolve the problem of abrupt network failure quickly. In this paper, we propose a near-optimal cell outage management (NoCOM) scheme to support seamless services to users. In consideration of system throughput, user fairness, and the guarantee of QoS simultaneously, the NoCOM scheme finds the solution of subchannel and power allocations using a non-convex optimization technique and allocates radio resources to users iteratively. Through intensive simulations, we verify the outstanding performances of the proposed NoCOM scheme with respect to the average cell capacity, user fairness, and computational complexity.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.5
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• pp.2124-2143
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• 2016

In Long Term Evolution-Advanced (LTE-A) systems, Inter-cell Interference (ICI) is a prominent limiting factor that affects the performance of the systems, especially at the cell edges. Based on the literature, Fractional Frequency Reuse (FFR) methods are known as efficient interference management techniques. In this report, the proposed Dynamic Fractional Frequency Reuse (DFFR) technique improved the capacity and cell edge coverage performance by 70% compared to the Fractional Frequency Reuse (FFR) technique. In this study, an improved power allocation method was adopted into the DFFR technique to reach the goal of not only reducing the ICI mitigation at the cell edges, but also improving the overall capacity of the LTE-A systems. Hence, an improved water-filling algorithm was proposed, and its performance was compared with that of other methods that were considered. Through the simulation results and comparisons with other frequency reuse techniques, it was shown that the proposed method significantly improved the performance of the cell edge throughput by 42%, the capacity by 75%, and the coverage by 80%. Based on the analysis and numerical expressions, it was concluded that the proposed DFFR method provides significant performance improvements, especially for cell edge users.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.5
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• pp.812-814
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• 2015

In this letter, a distributed self-organized cell association scheme is proposed for heterogeneous cellular networks. In which each small cell uses a common self-organization strategy and takes its environment into account to establish its own cell association scheme. It is confirmed that the proposed cell association improves the user throughput and areal spectral efficiency compared to joint association.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.2A
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• pp.179-187
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• 2007

As wireless networks evolve to orthogonal frequency division multiple access(OFDMA) systems, inter-cell interference control becomes a critical issue in radio resource management. The allocation of the same channels in neighbor cells cause inter-cell interference, so the channel allocation needs to be taken carefully to lower the inter-cell interference. In distributed channel allocation, each cell independently tries to allocate channels that suffer low interference level. In this paper, under the assumption of static users, we introduce the concept of interference range and use it in designing our two algorithms; basic and combined. The basic algorithm performs interference range detection and determines whether to use the considered channel, while the combined algorithm checks the channel quality in addition to detecting the interference range. The two algorithms dynamically perform channel allocation with low complexity and show good throughput and fairness performance.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.2
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• pp.288-307
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• 2013

This paper formulates resource allocation for decode-and-forward (DF) relay assisted multi-cell orthogonal frequency division multiple (OFDM) networks as an optimization problem taking into account of inter-cell interference and users fairness. To maximize the transmit rate of system we propose a joint interference coordination, subcarrier and power allocation algorithm. To reduce the complexity, this semi-distributed algorithm divides the primal optimization into three sub-optimization problems, which transforms the mixed binary nonlinear programming problem (BNLP) into standard convex optimization problems. The first layer optimization problem is used to get the optimal subcarrier distribution index. The second is to solve the problem that how to allocate power optimally in a certain subcarrier distribution order. Based on the concept of equivalent channel gain (ECG) we transform the max-min function into standard closed expression. Subsequently, with the aid of dual decomposition, water-filling theorem and iterative power allocation algorithm the optimal solution of the original problem can be got with acceptable complexity. The third sub-problem considers dynamic co-channel interference caused by adjacent cells and redistributes resources to achieve the goal of maximizing system throughput. Finally, simulation results are provided to corroborate the proposed algorithm.

• BMB Reports
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• v.45 no.4
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• pp.233-238
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• 2012

We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome.

• ETRI Journal
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• v.42 no.2
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• pp.175-185
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• 2020

Inter-cell interference (ICI) is a major problem in heterogeneous networks, such as two-tier femtocell (FC) networks, because it leads to poor cell-edge throughput and system capacity. Dynamic ICI coordination (ICIC) schemes, which do not require prior frequency planning, must be employed for interference avoidance in such networks. In contrast to existing dynamic ICIC schemes that focus on homogeneous network scenarios, we propose a novel semi-distributed dynamic ICIC scheme to mitigate interference in heterogeneous network scenarios. With the goal of maximizing the utility of individual users, two separate algorithms, namely the FC base station (FBS)-level algorithm and FC management system (FMS)-level algorithm, are employed to restrict resource usage by dominant interference-creating cells. The distributed functionality of the FBS-level algorithm and low computational complexity of the FMS-level algorithm are the main advantages of the proposed scheme. Simulation results demonstrate improvement in cell-edge performance with no impact on system capacity or user fairness, which confirms the effectiveness of the proposed scheme compared to static and semi-static ICIC schemes.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.11
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• pp.12-20
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• 2013

Even though extensive research results have been applied to wireless cellular systems to improve their capacity and coverage, severe performance degradation experienced in cell boundary areas still remains as a major limiting factor to prohibit further improvement of user equipment (UE) throughput. In the Long Term Evolution-Advanced (LTE-A) standard of the Third Generation Partnership Project (3GPP), Some advanced techniques have been introduced to overcome this "cell-edge problem", including coordinated multipoint transmission and reception (CoMP) and inter-cell interference coordination (ICIC). In this paper, we propose yet another strategy to improve the performance of low-tier UEs by using the concept of multiple beam direction patterns (BDPs). Such multiple BDPs can be implemented using multi-layer antenna arrays stacked vertically at base station (BS) sites to transmit signals in different main beam directions. In comparison to conventional three-sector antennas with a fixed beam pattern, the proposed methods makes signal transmission in a rotational fashion to significantly enhance the reception quality of UEs located near sector (or cell) edge areas, preventing the situation where certain UEs are marginally covered by the BS for the whole transmission time. Performance evaluation results show that the proposed scheme outperforms the conventional three-sector transmission by 171% in low 5% UEs in terms of the UE throughput.