• Archives of Pharmacal Research
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• 제3권1호
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• pp.7-12
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• 1980

Ampliciline was synthesized from 6-amino-pencillanic acid (6-APA) and D-.alpha. phenylglycine methyl ester by using amplicilin synthesizing enzyme from Peudomonas melanogenum (IAM 1655). The whole cell enzyme was immobilized by entrapping it in the polyacrylamide gel lattices. The polymer used in the enzyme entrapment was made from 150 mg per ml of acrylamide monomer and 8 mg per ml of N, N'-methylenebisacrylamide. About 200 mg/whole cell enzyme was mixed in the polymer for entrapment. The maximal activity retention after immobilization was 56%. The optimal pH values for the whole cell enzyme and the immobilized whole cell enzyme were 6.0 and 5.9, respectively. The optimal temperature for the enzyme activity were the same for both type of preparations. The enzyme stabilities against pH and heat increased for immobilized whole cell enzyme. Immobilized cell was more stable especially in the acidic condition while both type were found to be very suceptible to thermal inactivation at a temperature above 4.deg.C. The kinetic constants obtained from Lineweaver-Burk plot based on two substate reaction mechanism showed somewhat higher value for immobilized whole cell enzyme as compared to the whole cell enzyme : the Km value for 6-APA were 7.0 mM and 12.5 mM while Km values for phenylglycine methyl ester were 4.5 mM and 8.2 mM, respectively. Using the immobilized whole cell enzyme packed in a column reactor, the productivity of ampiciline was studied by varying the flow rate of substrate solution. At the space velocity, SV, 0.14 hr$^{-1}$ the conversion was 45%. Operational stability found in terms of half life was 30 hr at SV = 0.2 hr.

• Journal of Electrochemical Science and Technology
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• 제12권4호
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• pp.406-414
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• 2021

Electrochemical devices are constructed for continuous syngas (CO + H₂) production with controlled selectivity between CO₂ and proton reduction reactions. The ratio of CO to H₂, or the faradaic efficiency toward CO generation, was mechanically manipulated by adjusting the space volume between the cathode and the polymer gas separator in the device. In particular, the area added between the cathode and the ion-conducting polymer using 0.5 M KHCO₃ catholyte regulated the solution acidity and proton reduction kinetics in the flow cell. The faradaic efficiency of CO production was controlled as a function of the distance between the polymer separator and cathode in addition to that manipulated by the electrode potential. Further, the electrochemical CO₂ reduction device using Au NPs presented a stable operation for more than 23 h at different H₂:CO production levels, demonstrating the functional stability of the flow cell utilizing the mechanical variable as an important operational factor.

• BMB Reports
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• 제49권1호
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• pp.37-44
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• 2016

Spectraplakins are crucially important communicators, linking cytoskeletal components to each other and cellular junctions. Microtubule actin crosslinking factor 1 (MACF1), also known as actin crosslinking family 7 (ACF7), is a member of the spectraplakin family. It is expressed in numerous tissues and cells as one extensively studied spectraplakin. MACF1 has several isoforms with unique structures and well-known function to be able to crosslink F-actin and microtubules. MACF1 is one versatile spectraplakin with various functions in cell processes, embryo development, tissue-specific functions, and human diseases. The importance of MACF1 has become more apparent in recent years. Here, we summarize the current knowledge on the presence and function of MACF1 and provide perspectives on future research of MACF1 based on our studies and others. [BMB Reports 2016; 49(1): 37-44]

• International Journal of Aeronautical and Space Sciences
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• 제16권2호
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• pp.311-324
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• 2015

This study presents computational analyses for low-drag aerodynamic design that are applied to modify a long-endurance UAV. EAV-2 is a test-bed for a hybrid electric power system (fuel cell and solar cell) that was developed by the Korean Aerospace Research Institute (KARI) for use in future long-endurance UAVs. The computational investigation focuses on designing a wing with a reduced drag since this is the main contributor of the aerodynamic drag. The airfoil and wing aspect ratio of the least drag are defined, the fuselage configuration is modified, and raked wingtips are implemented to further reduce the profile and induced drag of EAV-2. The results indicate that the total drag was reduced by 54% relative to EAV-1, which was a small-sized version that was previously developed. In addition, static stabilities can be achieved in the longitudinal and lateral-directional by this low-drag configuration. A long-endurance flight test of 22 hours proves that the low-drag design for EAV-2 is effective and that the average power consumption is lower than the objective cruise powerof 200 Watts.

• 한국항공우주학회지
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• 제30권2호
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• pp.21-29
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• 2002

각 수치기법별로 충격파 주위에서 발생하는 진동현상의 원인을 분석하여 단조성을 유지하면서 충격파를 정확하게 포착할 수 있는 M-AUSMPW+를 개발하였다. 제어면과 음속천이 점 위치에 대하여 FVS계열 수치 기법과 ASUM계열 수치기법들이 충격파 포착시 나타내는 특성들을 분석하였고 충격파 주위의 수치진동의 원인을 찾아내었다. 음속천이점의 위치를 계산하고 이를 통해 충격파 영역에서의 물리 현상을 정확하게 반영함으로써 기존 AUSM계열 수치기업이 가지는 충격과 주위의 물성치 진동현상을 근본적으로 제거 할 수 있었다.

• 한국항공우주학회지
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• 제35권8호
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• pp.677-684
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• 2007

본 연구에서는 삼차원 점성 유동을 효율적으로 해석하기 위해 사면체, 프리즘, 피라미드를 포함하는 비정렬 혼합격자계를 기반으로 하는 유동해석코드를 개발하였다. 유동의 지배방정식은 격자점 중심의 유한체적법을 사용하여 공간차분회었으며, 제어테적은 메디안 듀얼(median-dual)방법으로 구성하였다. 난류유동 해석은 Spalart-Allmaras 난류모형과 연계하여 계산되었다. 개발된 해석코드의 정상 유동 검증을 위해 삼차원 날개에 대한 층류, 난류유동을 해석하였으며, 비정상 유동 검증을 위해 조화운동에 의해 진동하는 삼차원 날개에 대한 유동해석을 수행하였다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제10권4호
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• pp.316-324
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• 2010

In this paper, we design a 256 kbits EEPROM for a MCU (Microcontroller unit) with the wide voltage range of 1.8 V to 5.5 V. The memory space of the EEPROM is separated into a program and data region. An option memory region is added for storing user IDs, serial numbers and so forth. By making HPWs (High-voltage P-wells) of EEPROM cell arrays with the same bias voltages in accordance with the operation modes shared in a double word unit, we can reduce the HPW-to-HPW space by a half and hence the area of the EEPROM cell arrays by 9.1 percent. Also, we propose a page buffer circuit reducing a test time, and a write-verify-read mode securing a reliability of the EEPROM. Furthermore, we propose a DC-DC converter that can be applied to a MCU with the wide voltage range. Finally, we come up with a method of obtaining the oscillation period of a charge pump. The layout size of the designed 256 kbits EEPROM IP with MagnaChip's 0.18 ${\mu}m$ EEPROM process is $1581.55{\mu}m{\times}792.00{\mu}m$.

• 한국항공우주학회지
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• 제35권2호
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• pp.94-100
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• 2007

초음속 제트 마하수 1.07부터 1.2 범위에서 축대칭 제트 스크리치 톤을 해석하였다. 축대칭 모드는 낮은 마하수 축대칭 제트의 지배적인 스크리치 톤 모드이다. 난류 해석을 위해 수정된 Spalart-Allmaras 모델을 RANS (Reynolds-averaged Navier-Stokes) 방정식에 사용하였다. 스크리치 톤 해석에서 중요한 음파의 전파, shock-cell 구조 및 거대한 불안정 파를 정확히 계산하기 위해 비반사 특성 경계조건과 연계한 고차정확도의 ENO 기법을 사용하였다. 수치 해석결과는 다른 연구자들의 실험 및 계산 결과와 잘 일치하였으며, 따라서 본 연구에 사용된 수치 기법들이 초음속 제트 유동 및 소음연구에 타당함을 확인하였다.

• Journal of Astronomy and Space Sciences
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• 제29권1호
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• pp.79-84
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• 2012

After a visit by Peter Waddell from the University of Strathclyde, Glasgow, UK in 1991, Robert H. Koch launched a program at the University of Pennsylvania to build lightweight pneumatic membrane mirrors, initially for balloon flight observations where weight is at a premium. Mirror cells were fabricated from sizes 0.18 m to 1.77 m, and experiments conducted to characterize the mirror figure and stability. Most of the work stopped after Prof. Koch's retirement in 1996 until 2006 when the authors expressed an interest in building an array of medium-aperture portable telescopes. The program restarted in earnest at Gravic, Inc. in Malvern, PA in 2008 with Koch using his extensive observational astronomy experience to guide the fabrication of a fully operational 1.07 m membrane mirror telescope with an optical tube assembly weighing under 45 Kg. Residual wavefront aberrations remediation resulted in Koch and the authors investigating membrane tensioning techniques with different cell designs, active secondary wavefront correction, photometric algorithms for aberrated images, and the use of additional lightweight mirror substrates from the Alt-Az Initiative Group, such as foamed glass. The best result for the lightweight mirrors was a point spread function spot size of several arc seconds. A lightweight 1.6 m cast aluminum cell alt-az telescope was subsequently designed by Koch and the authors for prime focus use.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2008년도 추계학술발표대회 논문집
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• pp.232-237
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• 2008

The integration of PV modules into building facades or roof could raise their temperature that results in the reduction of PV system's electrical power generation. Hot air can be extracted from the space between PV modules and building envelope, and used for heating in buildings. The extraction of hot air from the space will enhance the performance of BIPV systems. The solar collector utilizing these two aspects is called PV/T(photovoltaic/thermal) solar collector. This research is about the development of solar roof system with PV/T collector to apply into buildings. A test cell experiment was performed with the PVT roof installed: It found that the hot air supply from the PVT air collector contributed to increase the heating efficiency by 2 times and the electrical efficiency by about 8%.