• Archives of Plastic Surgery
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• 제49권3호
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• pp.423-426
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• 2022

Reconstruction of the upper lateral lip subunit is challenging, and use of several classical local flaps have been previously reported. However, these methods have drawbacks such as visible scarring, anatomic distortion, and functional disability. To obtain satisfactory results, preservation of perioral function is important. We report a case of functional upper lip reconstruction after tumor resection using a reverse facial-submental artery island flap with a reinnervated anterior belly of the digastric muscle (ABDM) without sacrificing the perioral structure. A 73-year-old man presented with basal cell carcinoma on the left upper lip which was widely excised, including the orbicularis oris muscle. The remaining 4 cm × 3.5 cm defect was reconstructed using a reverse facial-submental artery island flap with ipsilateral ABDM. The motor nerve of the ABDM was sutured with the stump of the buccal branch of the ipsilateral facial nerve. The postoperative course was uneventful, and good functional and esthetic recovery were observed at 12-month follow-up. This procedure may be an alternative option for reconstruction of lateral upper lip defects.

• 한국식품영양학회지
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• 제22권2호
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• pp.313-319
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• 2009

To improve the growth of human mesenchymal stem cells(hMSCs) under general cell culture conditions(20% $O_2$ and 5% $CO_2$), we examined the effect of s-allylcysteine(SAC), which is known as an antioxidant and the main component of aged-garlic extract, on hydrogen peroxide-induced cellular stress in hMSCs. We found that SAC blocked hydrogen peroxideinduced cell death and cellular apoptosis, but that SAC did not improve the growth of hMSCs during short-term culture. To evaluate the protective effect of SAC, we examined the endogenous expression of the antioxidant enzymes catalase (CAT), superoxide dismutase(SOD), and glutathione peroxidase(Gpx) in hMSCs. Hydrogen peroxide was found to downregulate the expression of CAT, SOD, and Gpx at the protein level. However, in the pre-treatment group of SAC, SAC inhibited the hydrogen peroxide-induced down-regulation of CAT, SOD, and Gpx. Unfortunately, treatment with SAC alone did not induce the up-regulation of antioxidant enzymes and the cell proliferation of hMSCs. Surprisingly, SAC improved cell growth in a single cell level culture of hMSCs. These results indicate that SAC may be involved in the preservation of the self-renewal capacity of hMSCs. Taken together, SAC improves the proliferation of hMSCs via inhibition of oxidative-stress-induced cell apoptosis through regulation of antioxidant enzymes. In conclusion, SAC may be an indispensable component in an in vitro culture system of human MSCs for maintaining self-renewal and multipotent characterization of human MSCs.

• Journal of Chest Surgery
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• 제12권4호
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• pp.383-394
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• 1979

Treatment of valvular heart disease with valve replacement has been one of the most popular procedures in cardiac surgery recently. Although, first effort was directed toward the prosthetic valve, it soon became popular that bioprosthesis, the valvular xenograft, was prefered in the majority cases. Valvular xenograft has some superiority to the artificial prosthetic valve in some points of thromboembolism and hemolytic anemia, and it also has some inferiority of durability, immunologic reaction and resistance to Infection. Tremendous efforts were made to cover the inferiority with several methods of collection, preservation, and valve mounting of the porcine valve or pericardium of the calf, and also with surgical technique of the valvular xenograft replacement. Auther has collected 320 porcine aortic valves immediately after slaughter, and aortic cusps were coapted with cotton balls in the Valsalva sinuses to protect valve deformity after immersion in the Hanks' solution, and oxidation, cross-linking and reduction procedures were completed after the proposal of Carpentier in 1972. Well preserved aortic valves were suture mounted in the hand-made tissue valve frame of 19, 21, and 23 mm J.d., and also in the prosthetic vascular segment of 19 mm Ld. with 4-0 nylon sutures after careful trimming of the aortic valves. Completed valves were evaluated with bacteriologic culture, pressure tolerance test with tolerane gauge, valve durability test in the saline glycerine mixed solution with tolerance test machine in the speed of 300 rpm, and again with pathologic changes to obtain following results: 1. Bacteriologic culture of the valve tissue in five different preservation method for two weeks revealed excellent and satisfactory result in view of sterilization including 0.65% glutaraldehyde preservation group for one week bacteriologic culture except one tissue with Citobacter freundii in 75% ethanol preserved group. 2. Pressure tolerance test was done with an apparatus composed of V-connected manometer and pressure applicator. Tolerable limit of pressure was recorded when central leaking jet of saline was observed. Average pressure tolerated in each group was 168 mmHg in glutaraldehyde, 128 mmHg in formaldehyde, 92 mmHg in Dakin's solution, 48 mmHg in ethylene oxide gas, and 26 mmHg in ethanol preserved group in relation to the control group of Ringer's 90 mmHg respectively. 3. Prolonged durability test was performed in the group of frame mounted xenograft tissue valve with 300 up-and-down motion tolerance test machine/min. There were no specific valve deformity or wearing in both 19, 21, and 23 mm valves at the end of 3 months (actually 15 months), and another 3 months durability test revealed minimal valve leakage during pressure tolerance test due to contraction deformity of the non-coronary cusp at the end of 6 months (actually 30 months) in the largest 23 mm group. 4. Histopathologic observation was focussed in three view points, endothelial cell lining, collagen and elastic fiber destructions in each preservation methods and long durable valvular tolerance test group. Endothel ial cell lining and collagen fiber were well preserved in the glutaraldehyde and formaldehyde treated group with minimal destruction of elastic fiber. In long durable tolerance test group revealed complete destruction of the endothelial cell lining with minimal destruction of the collagen and elastic fiber in 3 month and 6 month group in relation to the time and severity. In conclusion, porcine xenograft treated after the proposal of Carpentier in 1972 and preserved in the glutaraldehyde solution was the best method of collection, preservation and valve mounting. Pressure tolerance and valve motion tolerance test, also, revealed most satisfactory results in the glutaraldehyde preserved group.

• 한국식품저장유통학회지
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• 제16권6호
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• pp.804-809
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• 2009

최근, 저온 장시간 발효된 묵은지에 대한 소비가 증가하고 있다. 본 논문에서는, 저온($5^{\circ}C$)에서 46주 동안 발효된 묵은지의 pH, 산도, 유기산, 생균수, 아미노산, polygalacturonase (PG) 활성 등을 분석하였다. 10주 발효한 김치에서는 pH 4.1, 산도 0.99%를 나타낸 반면, 46주 발효한 김치에서는 pH 3.9, 산도 1.29%를 보였다. 한편, 10주에서 46주로 발효 기간이 증가하면서, 젖산, 젖산/초산비, Lactobacillus species/Leuconostoc species비 등은 증가한 반면, 호기성 세균, 효모, Lactobacillus species, Leuconostoc species, 유리아미노산, PG 효소 활성은 감소하였다.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.206-212
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• 2001

Water extract of green tea(GTW) and 70% ethanol extract of green tea(GTE) were prepared for the test of antibacterial activity. The sensitivity of Staphylococcus aureus and Salmonella typhimurium to the green tea extracts in various pH of culture broth was tested. Tryptic soy broth(TSB) containing 0∼2%(w/v) of green tea extracts was adjusted to pH 5.0∼7.0 and inoculated with 10$\^$5/∼10$\^$6/ cells/ml of each bacteria. The plate counting method and clear zone test were used to determine inhibitory effect of green tea extracts. Green tea extracts completely inhibited the growth of S. aureus at 0.5% level and bactercidal at 0.5∼1.0% level of GTW and GTE at pH 5.0∼7.0. Green tea extracts were bactercidal to S. typhimurium at 1.5∼2.0% level of GTW and 1.0∼2.0% level of GTE at pH 7.0. Sal. typhimurium was more resistant than S. aureus. in same concentration of green tea extracts at same pH. The resistance of S. aureus and Sal. typhimurium was increased with decreasing pH of culture broth. The morphology of S. aureus cells treated with green tea extracts showed damage of cell wall and cytoplasmic membrane. Severely damaged cells of S. aureus lost electron dense material and cytoplasm. Green tea extracts stimulated autolysis and cell death of S. aureus. This result suggests that green tea extracts can be used as an effective natural antibacterial agent in food.

• 한국식품저장유통학회지
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• 제10권1호
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• pp.70-74
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• 2003

벼의 산지 및 품종별로는 중온 및 고온성 세균의 분포에 큰 차이가 없었으며, 도정도가 낮을수록 저장기간이 길수록 각각의 균들이 많이 존재하였다. 전기밥솥에서 고온성 세균의 증식은 75$^{\circ}C$이하에서 보온온도가 낮을수록 빠르게 나타났으며, 보온 초기에는 나타나지 않았으나 18∼24시간 사이에 증식속도가 빨라지는 경향을 나타내었다. 전기밥솥 밥의 고온성 세균수와 이상취 발생의 상관성은 있었으며, 고온성 세균수는 오븐내 밥의 표면중앙이 중심이나 밑바닥에 비하여 약간 많게 나타났다. 백미밥의 휘발성 성분은 보온초기에는 쌀로부터 유래되는 hexanal이 주성분이었으며, 장시간보온 후는 이상취를 나타내는 furan을 포함한 고비점 물질이 많았다.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.253-258
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• 2009

This study investigated the effects of mycelium and culture supernatant of Cordyceps ochraceostromat(Co) on air way hyper-responsiveness, pulmonary immune cell infiltration, and Th2 cytokine expression in animal models of atopy and asthma. After ConA(+/-) activation of mouse primary spleen cells, decreased IL-4 and IL-13 cytokine production were seen in the presence of Co mycelium extracts and culture supernatant. The asthma model involved mice sensitized to ovalbumin by i.p. injection treatment; Co mycelium extract was also injected. The atopy model was the dinitrofenylbenzene-treated mouse ear. Ear thicken ing induced by DNFB was decreased by Co mycelium extract, and the extract also inhibited lung cell infiltration in ovalbumin-induced asthmatic mice. The results thus indicated that the Co mycelial extract reduced the undesirable immune responses seen in asthma and atopy.

• 한국식품저장유통학회지
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• 제21권3호
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• pp.301-307
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• 2014

본 연구 결과 울금 에탄올 추출물이 DMBA에 의해 유도한 유선 암화과정에서 종양의 발생률과 종양수를 감소시킴을 확인하였다. 이러한 종양세포의 증식 억제 효과는 울금의 암 예방 효과에 대한 기본 메커니즘 중 하나이다. 앞으로 효과적인 투여 경로 및 조직 특이성을 검토하기 위하여 더 많은 연구가 필요하다고 사료된다.

• 한국식품저장유통학회지
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• 제13권4호
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• pp.495-500
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• 2006

항산화물에 의한 천식 억제 효과를 확인하기 위하여 항산화정도를 알아볼 수 있는 DPPH, FRAP, hydroxyl radical 소거활성 실험을 high throughput-compatible chemical assay로 디자인하여 해바라기씨 추출물에 적용하여 보았다. 해바라기씨 추출물의 용매에 따른 항산화 효과 중 $H_2O_2$에 의한 산화스트레스에 의한 세포사멸을 DW추출물에서 강한 억제능력을 보였다. 이를 이용하여 마우스를 이용한 천식 동물모델에 적용하여 탁월한 천식 억제효과를 $CD4^+$세포, IL-4와 IL-13의 발현정도를 통해 알수 있었다. 해바라기씨 추출물은 천식에 탁월한 효과가 있는 것으로 in vitro 및 in vivo 실험으로 증명하였다.

• 한국식품저장유통학회지
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• 제15권1호
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• pp.105-110
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• 2008

대나무수액의 자유라디칼 소거능을 측정하기 위하여 DPPH를 이용한 자유 라디칼 소거능 실험을 수행하여 항산화 효과를 측정한 결과 대나무수액의 농도가 높을수록 DPPH 활성이 뛰어남을 알 수 있었고, ROS를 이용하여 항산화효과를 확인하였다. 배양된 대식세포에 대나무수액을 농도별로 첨가한 결과 대나무수액 농토가 높을수록 과산화수소에 의해 유도된 산화적 자극이 감소하였다. 또한 세포 생존에 미치는 영향을 알고자 대나무수액을 농도별로 첨가하여 24시간 후 세포의 형태변화를 HIT assay로 실시한 결과 산화적 자극에 의해 발생한 세포손상이 대나무수액의 농도가 높아질수록 감소하는 것이 확인되었다. Ascorbic acid는 $H_2O_2$에 의해 야기되는 세포독성을 억제해 주는 효과가 10 %로 우수하게 나타났고 대나무수액 역시 고농도에서 27 %의 세포손상 방지효과가 우수하였다. 따라서 대나무수액은 안전하고 독성이 전혀 없는 천연 항산화제로서의 가능성을 보였다.