• 대한화학회지
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• 제67권2호
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• pp.137-144
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• 2023

In this study were evaluated the whitening and anti-oxidative activities from the extracts of Sorbus alnifolia branches, and identified the chemical structures of the active ingredients. In the whitening tests using α-MSH stimulated B16F10 melanoma cells, the 70% ethanol extract and n-butanol (n-BuOH) fractions concentration-dependently inhibited cellular melanogenesis and intracellular tyrosinase activities without causing cell toxicity. The total polyphenol content of n-BuOH and ethyl acetate (EtOAc) fractions were measured to be respectively 241.1 ± 1.1 and 222.9 ± 2.4 (mg/g GAE), and the total flavonoid content of EtOAc fraction was 75.3 ± 2.0 (mg/g QE). Upon anti-oxidant studies with DPPH and ABTS⁺ radicals, potent radical scavenging activities were observed in the EtOAc and n-BuOH fractions. Moreover, in the study of cell protection efficacy using HaCaT keratinocytes damaged by H₂O₂, the EtOAc and n-BuOH fractions showed a very positive results on prevention of oxidative stress. Phytochemical studies for this extract resulted in the isolation of four compounds; 2-oxopomolic acid (1), euscaphic acid (2), epi-catechin (3), prunasin (4). These results suggested that the extract of S. alnifolia branches containing compounds 1-4 as natural ingredients could be used as whitening and anti-oxidant ingredients in cosmetic formulations.

• 한국임상수의학회지
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• 제40권5호
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• pp.323-329
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• 2023

Peripheral blood-derived mesenchymal stem cells (PB-MSCs) have shown promise in cell-based therapy, as they can be harvested with ease through minimally invasive procedures. This study aimed to isolate PB-MSCs from foals and mares and to compare the proliferation and cellular characteristics of the PB-MSCs between the two groups. Six pairs of mares and their foals were used in this study. MSCs were isolated from PB by direct plating in a tissue culture medium, and cell proliferation (population doubling time [PDT], and colony-forming unit-fibroblast assay [CFU-F]), and characterization (morphology, plastic adhesiveness, colony formation, trilineage differentiation) were examined. There was no significant difference in the PB-MSC yield, CFU-F, and PDT between the mares and foals. PB-MSCs from both mares and foals showed typical MSC characteristics in terms of spindle-shaped morphology, plastic adhesive properties, formation of colonies, trilineage differentiation. These results suggest that PB-MSCs isolated from horses, both adult horses, and foals, can be used for equine cell-based therapy.

• 생명과학회지
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• 제9권5호
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• pp.531-536
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• 1999

As an attempt to preserve resources in marine biological organisms, we first constructed a cDNA library from the red alga Porphyra yezoensis. The library construction method from P. yezoensis consists of three steps; those include protoplast presparation, RNA isolation, and phage library construction. Protoplast was prepared in order to remove much of the carbohydrate compounds which are characteristics of algal cell walls. Carbohydrate contamination in the purified RNA may inhibit further enzyme reactions, those carbohydrates should be removed. RNA samples prepared from protoplast still seemed to contain residual amount of carbohydrate because mRNA isolation with conventional method failed. We therefore developed a method with Poly ATtract mRNA isolation system. The constructed phage library was tested by analyzing cDNA insert in phage vector from randomly picked ten independent white plagues. All of the phages contained cDNA inserts with sizes ranging 0.5kb and 2.0kb.

• 생약학회지
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• 제50권4호
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• pp.245-252
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• 2019

This study was initially explored to procure biomaterials capable of inhibiting cancer cell growth from nine Persicaria species (Polygonaceae). The extract of P. nepalensis that was selected from the initial screenings was further fractionated to identify bioactive compounds. The ethyl acetate (EtOAc) fraction was shown to be the most active in the inhibition of cell growth against six cancer cell lines (IC₅₀ value of 3.77-12.87 ㎍/ml). Phytochemical study led to the isolation of two galactolipids of 1,2-di-O-linolenoyl-3-O-β-D-galactospyranosyl-sn-glycerol (1) and 1-O-linolenoyl-3-O-β-D-galactospyranosyl-sn-glycerol (2) from the hexane fraction and three phenylpropanoyl sucroses of lapathoside A (3), vanicoside B (4) and lapathoside C (5) from the EtOAc fraction. These isolated compounds have not been reported from this plant. Compounds 3 and 4 exhibited the effective growth inhibition against a panel of cancer cell lines (IC₅₀ value of 6.90-18.09 μM). In addition, the anti-inflammatory activity was evaluated to determine lipopolysaccharide (LPS)-induced nitric oxide (NO) formation in RAW264.7 mouse macrophage cells. The EtOAc fraction (IC₅₀; 34.14 ㎍/ml) and its constituents, 3 (8.55 μM) and 4 (7.83 μM) were shown to be effective in the inhibition of LPS-induced NO production. Therefore, compounds 3 and 4 were considered to be active constituents for anti-inflammatory and antitumor activity from P. nepalensis.

• 인터넷정보학회논문지
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• 제18권4호
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• pp.89-97
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• 2017

본 논문은 스마트 교육을 위한 청소년의 우울과 휴대폰 의존도, 부모의 양육행동, 또래관계의 구조적 관계를 확인하고자 하였다. 또 청소년의 우울과 휴대폰 의존 관계에서 부모양육행동과 또래관계의 매개적 효과를 확인하였다. 본 연구는 한국아동, 청소년패널 조사(KCYPS) 6차년도 중1패널의 자료 중 총 1897명의 청소년 응답과 보호자의 설문데이터를 분석대상으로 하였다. 자료분석을 위해 SPSS와 Amos프로그램을 사용하였다. 대상 변인들의 상관 및 기술통계분석을 위해서 SPSS을 사용하였고 부모의 양육행동의 경로분석과 매개효과 검증을 위해 구조방정식을 이용하였다. 검증결과, 청소년의 우울과 부모의 양육행동은 청소년의 휴대폰 의존도에 직접적인 영향을 미치는 것으로 나타났다. 그리고 청소년의 우울은 부모의 양육행동을 통해 간접적인 영향을 미치는 것으로 나타나 부분 매개효과를 확인할 수 있었다. 하지만 또래관계의 매개효과는 나타나지 않았다. 이상의 결과로부터 청소년의 휴대전화 의존을 예방하기 위한 방안과 스마트 교육 개발시 유의점에 등에 대해 논의하였다.

• 한국버섯학회지
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• 제22권3호
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• pp.87-91
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• 2024

버섯의 세포벽 분해를 최적화하기 위한 효소 처리에 따른 원형질체 분리 효율을 분석한 결과는 다음과 같다. 최적 삼투완충액 선발을 위해 Sorbitol, Sucrose, Mannitol, KCl을 각각 0.6M, 1.2M의 두가지 농도로 나누어 비교했을 때 0.6M Sorbitol에서 26.7±3.3×10⁷/mL로 가장 높은 원형질체 분리 효율을 확인하였다. 또한 선발된 삼투완충액과 영지 균사의 반응 시간에 따른 영향을 확인한 결과에서는 4시간 동안 반응했을 때 가장 높은 효율을 보였다. 대체 효소의 경우 endo-, exo-Chitinase의 혼합 효소인 Chimax-N을 사용했을 때 기존의 Lysing enzyme과 Yatalase에 비해 더 높은 분리 효율을 보여 원형질체 분리를 위한 대체 효소로서의 가능성을 확인하였다.

• 대한전자공학회논문지
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• 제26권6호
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• pp.124-130
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• 1989

ITIC를 중심으로 고밀도 DRAM을 위한 획기적인 밀도 향상을 기할 수 있는 공정과정과 회로디자인의 기술 혁신에 대하여 지다이너 입장에서 논의하였다. 여기서 개발한 TETC라 부르는 DRAM은 trench 기술과 SEG기술을 이용하였는데 $n^+-polysilycon$인 storage 전극과 $n^+-source$ 전극이 self-con-tact되고 soft error 를 극복할 만큼 충분히 큰 정전용량을 갖으므로 절연 영역을 따라서 만든 수직의 캐패시터를 이용함으로써 셀 크기를 기존의 BSE cell구조에 비하여 약 30% 감소되었다.

• 한국콘텐츠학회논문지
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• 제5권6호
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• pp.129-137
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• 2005

저궤도 위성통신시스템의 성능분석은 다양한 요소로 다양하게 시도되고 있다. 본 논문은 저궤도 위성통신시스템의 이전까지 연구에 없었던 사용자 밀집도를 고려한 셀 내 단말기 분포에 따른 간섭의 변화를 살펴보고, 단말기 분포 평균 거리에 따른 최대 사용자 수를 살펴보았다. 또한 저궤도 이동 통신시스템에 사용하는 안테나 종류(tapered-apertured antenna gaussian antenna)의 스폿 빔 isolation값에 따른 간섭을 살펴보았다. 이러한 간섭을 저궤도 위성 통신 시스템의 채널용량에 대입하여, 중요한 파라미터(대역폭, 캐리어 수)를 가지고 성능분석을 하였다. tapered-apertured antenna와 gaussian antenna를 비교함으로써, 어느 안테나가 간섭에 더 효과적이고 최적인지 알아보고자 한다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제13권4호
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• pp.402-409
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• 2013

An innovative application of voltage-contrast (VC) inspection allowed inline detection of NMOS leakage in dense SRAM cells is presented. Cell sizes of SRAM are continual to do the shrinkage with bit density promotion as semiconductor technology advanced, but the resulting challenges include not only development of smaller-scale devices, but also intra-devices isolation. The NMOS leakage caused by the underneath n+/P-well shorted to the adjacent PMOS/N-well was inspected by the proposed electron-beam (e-beam) scan in which VC images were compared during the in-line process step of post contact tungsten (W) CMP (Chemical Mechanical Planarization) instead of end-of-line electrical test, which has a long response time. A series of experiments based on the mechanism for improving the intra-well isolation was performed and verified by the inline VC inspection. An optimal process-integration condition involved to the tradeoff between the implant dosage and photo CD was carried out.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.890-894
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• 2001

The isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.