• Title/Summary/Keyword: Cell division

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SAFETY EVALUATION OF ADENOVIRUS-MEDIATED P16 GENE TRANSFER BY USING MICROARRAY AND 2D/MALDI-TOF

  • Park, Misun;Hoil Kang;Jaehee Pyo;Sinae Lim;Seungwan Jee;Miok Eom;Taikyung Ryeom;Kim, Okhee
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.196-196
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    • 2002
  • p16INK4a tumor suppressor gene transfer in the non-small cell lung cancer cells by transduction of recombinant adenovirus (Ad5CMV-p16) resulted in significant inhibition of cancer cell growth (Anticancer Res., 1998, 18:3257-3261). As a safety concern, we have investigated gene and protein expression after transduction of adenoviral vector (Ad5CMV-p16) in human non-small cell lung cancer (A549) cells by using microarray and 2D gel electrophoresis/ MALDI-TOF.(omitted)

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Effects of Carthami Flos on Human Gastric Cancer Cells (홍화가 인체 위암세포에 미치는 효과)

  • Kim, Jung-A;Han, Song-Ee;Song, Ho-Joon;Chae, Han;Kwon, Young-Kyu;Kim, Byung-Joo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.3
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    • pp.466-470
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    • 2011
  • The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human gastric cancer cells. We used two kinds of human gastric cancer cell lines, such as AGS cells and MKN45 cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Also, we showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in AGS cells and doxorubicin in MKN45 cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Therefore, our findings provide insight into unraveling the effects of Carthami Flos in human gastric cancer cells and developing therapeutic agents against gastric cancer.

A Splice Variant of the C2H2-Type Zinc Finger Protein, ZNF268s, Regulates NF-κB Activation by TNF-α

  • Chun, Jung Nyeo;Song, In Sung;Kang, Dong-Hoon;Song, Hye Jin;Kim, Hye In;Suh, Ja Won;Lee, Kong Ju;Kim, Jaesang;Won, Sang
    • Molecules and Cells
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    • v.26 no.2
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    • pp.175-180
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    • 2008
  • $I{\kappa}B$ kinase (IKK), the pivotal kinase in signal-dependent activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$), is composed of multiple protein components, including IKK ${\alpha}/{\beta}/{\gamma}$ core subunits. To investigate the regulation of the IKK complex, we immunoaffinity purified the IKK complex, and by MALDI-TOF mass spectrometry identified a splice variant of zinc finger protein 268 (ZNF268) as a novel IKKinteracting protein. Both the full-length and the spliced form of the ZNF268 protein were detected in a variety of mammalian tissues and cell lines. The genes were cloned and expressed by in vitro transcription/translation. Several deletion derivatives, such as KRAB domain (KRAB) on its own, the KRAB/spacer/4-zinc fingers (zF4), and the spacer/4-zinc fingers (zS4), were ectopically expressed in mammalian cells and exhibited had different subcellular locations. The KRAB-containing mutants were restricted to the nucleus, while zS4 was localized in the cytosol. TNF-${\alpha}$-induced NF-${\kappa}B$ activation was examined using these mutants and only zS4 was found to stimulate activation. Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-${\alpha}$-induced NF-${\kappa}B$ activation by interacting with the IKK complex.

3-D Simulation of T-Shaped Electrode and Comparison of Results with Experiments

  • Shin, Yeong-Kyo;Hwang, Tae-Su;Kang, Seok-Dong;Park, Hun-Gun;Ryu, Jae-Hwa;Kim, Hyun-Chul;Shin, Seong-Won;Lee, Jae-Koo
    • Journal of Information Display
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    • v.3 no.2
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    • pp.13-18
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    • 2002
  • Numerical simulation is one of the most useful tools to study gas discharge phenomena that occur in alternating current plasma display panel (AC-PDP) cell. Most PDP cell simulations have been performed for two-dimensional cell, is cross-section along the address electrode. We developed a three-dimensional PDP simulator and applied it to a T-shaped electrode cell in order to show the effects of sustain electrode shape that cannot be included in two-dimensional simulation. The dependence of power consumption on electrode shape and area in the simulation showed the same trend as experiment.

Cytotoxic Effects of Methanol Extracts from Medicinal Plants on Cancer Cell Lines

  • Lee, Jeong-Ho;Chun, Hyun-Ja;Lee, Ki-Nam;Lim, Jin-A;Ryu, Hyeong-Won;Baek, Seung-Hwa
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.210.3-210.3
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    • 2003
  • This study was performed to determine the cytotoxic effect of methanol extract from medicinal plants. The cell viability was determined by the MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus Chinensis Bail showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Bail possessed a potential antitumorous agent

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Effects of Cell Cycle Regulators on the Cell Cycle Synchronization of Porcine induced Pluripotent Stem Cells

  • Kwon, Dae-Jin;Hwang, In-Sul;Kwak, Tae-Uk;Yang, Hyeon;Park, Mi-Ryung;Ock, Sun-A;Oh, Keon Bong;Woo, Jae-Seok;Im, Gi-Sun;Hwang, Seongsoo
    • Development and Reproduction
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    • v.21 no.1
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    • pp.47-54
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    • 2017
  • Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, $100{\mu}M$), roscovitine (ROSC, $10{\mu}M$), or olomoucine (OLO, $200{\mu}M$) for 12 h. Flow Cytometry (FACs) data showed that piPSCs in control were in G1 ($37.5{\pm}0.2%$), S ($34.0{\pm}0.6%$) and G2/M ($28.5{\pm}0.4%$). The proportion of cells at G1 in DAI group was significantly higher than that in control, while STA, ROSC and OLO treatments could not block the cell cycle of piPSCs. Both of viability and apoptosis were affected by STA and ROSC treatment, but there were no significantly differences between control and DAI groups. Real-Time qPCR and FACs results revealed that DAI treatment did not affect the expression of pluripotent gene, Oct4. In case of OLO, it did not affect both of viability and apoptosis, but Oct4 expression was significantly decreased. Our results suggest that DAI could be used for synchronizing piPSCs at G1 stage and has any deleterious effect on survival and pluripotency sustaining of piPSCs.

Comparative Analysis of the Developmental Competence of Three Human Embryonic Stem Cell Lines in Vitro

  • Kim, Sung-Eun;Kim, Byung-Kak;Gil, Jung-Eun;Kim, Suel-Kee;Kim, Jong-Hoon
    • Molecules and Cells
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    • v.23 no.1
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    • pp.49-56
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    • 2007
  • One of the goals of stem cell technology is to control the differentiation of human embryonic stem cells (hESCs), thereby generating large numbers of specific cell types for many applications including cell replacement therapy. Although individual hESC lines resemble each other in expressing pluripotency markers and telomerase activity, it is not clear whether they are equivalent in their developmental potential in vitro. We compared the developmental competence of three hESC lines (HSF6, Miz-hES4, and Miz-hES6). All three generated the three embryonic germ layers, extraembryonic tissues, and primordial germ cells during embryoid body (EB) formation. However, HSF6 and Miz-hES6 readily formed neuroectoderm, whereas Miz-hES4 differentiated preferentially into mesoderm and endoderm. Upon terminal differentiation, HSF6 and Miz-hES6 produced mainly neuronal cells whereas Miz-hES4 mainly formed mesendodermal derivatives, including endothelial cells, leukocyte progenitors, hepatocytes, and pancreatic cells. Our observations suggest that independently-derived hESCs may differ in their developmental potential.

Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture

  • Jo, You-Young;Kweon, HaeYong;Ji, Sang Deok;Kim, Jong Gil;Kim, Kee Young
    • Microbiology and Biotechnology Letters
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    • v.47 no.4
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    • pp.487-497
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    • 2019
  • Fetal Bovine Serum (FBS) is an essential substance added to animal cell culture medium. However, its composition is unclear causing problems such as development of an immune response when cultured cells are transplanted into the human body. In this study, silk sericin, silk fibroin, and hemolymph obtained from silkworms were added to the cell culture medium in order to determine if it can replace FBS. After establishment of the cell culture, cell proliferation and expression levels of cell growth-related genes were compared with those of control cells (cells cultured in the medium with 10% FBS). Results showed that the test group treated with silk fibroin extracted from a Korean silkworm variety, Kumokjam could replace 10% FBS. In addition, expression levels of cell growth related genes such as Fibronectin and TGF-β1 increased significantly in cells cultured using silk fibroin, depending on the concentration used in cell adhesion and cell proliferation [24]. To date, no studies have been conducted to find a replacement for FBS. Thus, this study was carried out to develop a substitute for FBS by using silkworm-derived alternatives such as silkworm hemolymph, silk sericin, and silk fibroin, which are cheap and have various physiological effects, cell promoting effects, and can be mass produced.

Cancer stem cell theory and update in oral squamous cell carcinoma (구강 편평세포암종에서의 암줄기세포 이론과 최신 지견)

  • Kim, Deok-Hun;Yun, Jun-Yong;Lee, Ju-Hyun;Kim, Soung-Min;Myoung, Hoon
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.37 no.2
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    • pp.97-108
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    • 2011
  • Cancer stem cells have stem cell-like features, such as the ability for self-renewal and differentiation but show unlimited growth because they have the lost normal regulation of cell growth. Cancer stem cells and normal stem cells have similar features. They show high motility, diversity of progeny, robust proliferative potential, association with blood vessels, immature expression profiles, nestin expression, epidermal growth factor (EGF)-receptor expression, phosphatase and tensin homolog (PTEN) expression, hedgehog pathway activity, telomerase activity, and Wnt pathway activity. On the other hand, with cancer cells, some of these signaling pathways are abnormally modified. In 1875, Cohnheim suggested the concept of cancer stem cells. Recently, evidence for the existence of cancer stem cells was identified. In 1994, the cancer stem cells' specific cell surface marker for leukemia was identified. Since then, other specific cell surface markers for cancer stem cells in solid tumors (e.g. breast and colon cancer) have been identified. In oral cancer, studies on cancer stem cells have been performed mainly with squamous cell carcinomas. Oral cancer specific cell surface markers, which are genes strongly expressed in oral cancer and cancer stem cell specific side populations, have been identified. Cancer stem cells are resistant to radiotherapy and chemotherapy. Therefore, to eliminate malignant tumors efficiently and reduce the recurrence rate, therapy targeting cancer stem cells needs to be performed. Currently, studies targeting the cancer stem cells' specific signaling pathways, telomerase and tumor vasculatures are being done.

Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus

  • Kim, Ju-Won;Oh, Bang Geun;Kim, Julan;Kim, Dong-Gyun;Nam, Bo-Hye;Kim, Young-Ok;Park, Jung Youn;Cheong, JaeHun;Kong, Hee Jeong
    • Development and Reproduction
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    • v.22 no.3
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    • pp.225-234
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    • 2018
  • A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.